Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (7): 1365-1371.doi: 10.3864/j.issn.0578-1752.2012.07.014

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Analysis on Composition and Content of Glucosinolate in Three Color Types of Maca(Lepidium meyenii)

 GAN Jin, FENG Ying, ZHANG Hong, HE Zhao, XU Long-Feng, CHEN Xiao-Ming   

  1. Research Institute of Resources Insect, Chinese Academy of Forestry / Engineering and Technology on Forest Resources with Characteristics, State Forestry Administration, Kunming 650224  
  • Received:2011-11-16 Online:2012-04-01 Published:2012-02-01

PDF

995

Cited

18

Abstract: 【Objective】 The composition and content of glucosinolate in three color types of maca (Lepidium meyenii) cultivated in Yunnan were analyzed in this study.【Method】HPLC and LC-ESI/MS were applied to analyze glucosinolate compositions and content of the methanol extracted from fresh, vacuum drying and air drying maca hypocotyls samples. 【Result】Two aromatic glucosinolates, benzyl glucosinolate and methoxybenzyl glucosinolate, were identified from three color types of maca. The total glucosinolate content in purple, yellow and white fresh samples was 50.14, 46.35, and 84.57 μmol•g-1, respectively. The total content in air drying samples was 13.05, 14.35, and 14.94 μmol•g-1, respectively, and the total content in vacuum drying samples was 0.24, 0.05, and 0.29 μmol•g-1, respectively. 【Conclusion】 Three color types of maca cultivated in Yunnan contain the same compositions of glucosinolate, the composition was consistent with that of sample cultivated in Peru. The difference in content was significant among three color types of fresh hypcotyle (P＜0.01), but all of them were much higher than that from Peru. The content of glucosinolate in white sample was the highest. The content of dried samples was much lower than that in fresh samples due to mostly hydrolyzed during the processing of drying.

Key words: maca (Lepidium meyenii), glucosinolate, analysis of composition and content, HPLC, LC-ESI/MS

[1]Gonzales G F, Valerio L G. Medicinal plants from Peru: a review of plants as potential agents against cancer. Anti-Cancer Agents in Medicinal Chemistry, 2006, 6:429-444.

[2]Rea J. Neglected crops: 1492 from a different perspective//Hernando B, Leon J. Plant Production and Protection Series No. 26. Rome ( Italy): FAO , 1994: 165-167.

[3]冯 颖, 何 钊, 徐珑峰, 张忠和, 石 雷, 陈晓鸣. 云南栽培玛咖的营养成分分析与评价. 林业科学研究, 2009, 22(5): 696 -700.

Feng Y, He Z, Xu L F, Zhang Z H, Shi L, Chen X M. Nutritive elements analysis and evaluation of maca (Lepidium meyenii) cultivated in Yunnan. Forest Research, 2009, 22(5): 696-700. (in Chinese)

[4]甘 瑾, 冯 颖, 何 钊, 徐珑峰, 张 弘, 陈晓鸣. 云南栽培3种颜色玛咖中总生物碱含量分析. 食品科学, 2010, 31(24): 415-419.

Gan J, Feng Y, He Z, Xu L F, Zhang H, Chen X M. Total alkaloids in maca (Lepidium meyenii) cultivated in Yunnan. Food Science, 2010, 31(24): 415-419. (in Chinese)

[5]何 钊, 冯 颖, 徐珑峰, 孙  龙, 石 雷, 陈晓鸣, 张忠和. 云南种植玛咖乙醇提取物的体外抗氧化活性分析. 食品科学, 2010,31(15): 39-43.

He Z, Feng Y, Xu L F, Sun L, Shi L, Chen X M, Zhang Z H. In vitro antioxidant activity of ethanol extract of maca (Lepidium meyenii Walpers) cultivated in Yunnan. Food Science, 2010, 31(15): 39-43. (in Chinese)

[6]Gonzales G F, Vasquez V, Rodriguez D, Maldonado C, Mormontoy J, Portella J, Pajuelo M, Villegas L, Gasco M. Effect of two different extracts of red maca in male rats with testosterone-induced prostatic hyperplasia. Asian Journal Andrology, 2007, 9(2): 245-251.

[7]Wade K L, Garrard I J, Fahey J W. Improved hydrophilic interaction chromatography method for the identification and quantification of glucosinolates. Journal of Chromatography, 2007, 1154: 469-472.

[8]Cicero A F G, Bandieri E, Arletti R. Lepidium meyenii Walp. improves sexual behaviour in male rats independently from its action on spontaneous locomotor activity. Journal of Ethnopharmacology, 2001, 75: 225-229.

[9]Gasco M, Villegas L, Yucra S, Rubio J, Gonzales G F. Dose-response effect of red maca (Lepidium meyenii) on benign prostatic hyperplasia induced by testosterone enanthate. Phytomedicine, 2007, 14:460-464.

[10]Vecera R, Orolin J, SKottova N, Kazdova L, Oliyarnik O, Ulrichova J, Simanek V. The influence of maca (Lepidium meyenii) on antioxidant status, lipid and glucose metabolism in rat.  Plant Foods for Human Nutrition, 2007, 62: 59-63.

[11]Ikeuchi M, Koyama T, Takei S, Kino T, Yazawa K. Effect benzylglucosinolate on endurance capacity in mice. Journal of Health Science, 2009, 55(2):178-182.

[12]Dini I, Tenore G C, Dini A. Glucosinolates from maca (Lepidium meyenii). Biochemical Systematics and Ecology, 2002, 30: 1087-1090.

[13]Piacente S, Carbone V, Plaza A, Zampelli A, Pizza C. Investigation of the tuber constituents of maca (Lepidium meyenii Walp.). Journal of Agricultural and Food Chemistry, 2002, 50: 5621-5625.

[14]Li G Y, Ammermann U, Quiros C E. Glucosinolate contents in maca (Lepidiumperuvianum Chacon) seeds, sprouts, mature plants and several derived commercial products. Economic Botany, 2001, 55(2): 255-262.

[15]Gasco M, Aguilar J, Gonzales G F. Effect of chronic treatment with three varieties of Lepidium meyenii (maca) on reproductive parameters and DNA quantification in adult male rats. Andrologia, 2007, 39: 151-158.

[16]Rubio J, Caldas M, Dávila S, Gasco M, Gonzales G F. Effect of three different cultivars of Lepidium meyenii (maca) on learning and depression in ovariectomized mice. Complementary and Alternative Medicine, 2006, 6:23.

[17]Rubio J, Dang H, Gong M, Liu X, Chen S L, Gonzales G F. Aqueous and hydroalcoholic extracts of Black maca (Lepidium meyenii) improve scopolamine-induced memory impairment in mice. Food and Chemical Toxicology, 2007, 45: 1882-1890.

[18]李培武，赵永国，张 文, 丁小霞, 杨 湄, 汪雪芳, 谢从华, 傅廷栋. 中国甘蓝型油菜硫苷含量及组份分析. 中国农业科学, 2005, 38(7): 1346-1352.

Li P W, Zhao Y G, Zhang W, Ding X X, Yang M, Wang X F, Xie C H, Fu T D. Analysis of glucosinolate components and profiles in Brassica napus. Scientia Agricultura Sinica, 2005, 38(7): 1346-1352. (in Chinese)

[19]Kiddle G, Bennett R N, Botting N P, Davidson N E, Robertson A A B, Wallsgrove R M. High-performance liquid chromatographic separation of natural and synthetic desulphoglucosinolates and their chemical validation by UV, NMR and chemical ionisation-MS methods. Phytochemical Analysis, 2001, 12: 226-242.

[20]腊贵晓, 方 萍, 李亚娟, 王 月. 液相色谱质谱联用分离、鉴定芥蓝中脱硫芥子油苷, 浙江大学学报: 农业与生命科学版, 2008, 34(5): 557-563.

La G X, Fang P, Li Y J, Wang Y. Determination of desulpho- glucosinolates in bolting stems of Chinese kale by liquid chromatography-mass. Journal of Zhejiang University: Agricultura & Life Science, 2008, 34(5): 557-563. (in Chinese)

[21]Matthaus B, Luftmann H. Glucosinolates in members of the family Brassicaceae: separation and identification by LC/ESI-MS-MS. Journal of Agricultural and Food Chemistry, 2000, 48(6): 2234-2239.

[22]季宇彬, 武晓丹, 邹 翔.硫代葡萄糖苷的研究. 哈尔滨商业大学学报: 自然科学版, 2005, 21(5): 550-562.

Ji Y B, Wu X D, Zou X. Study on progress of glucosinolates. Journal of Harbin University of Commerce: Natural Sciences Edition, 2005, 21(5): 550-562. (in Chinese)

[23]Gonzales G F, Miranda S, Nieto J, Fernández G, Yucra S, Rubio J, Yi P, Gasco M. Red maca (Lepidium meyenii) reduced prostate size in rats . Reproductive Biology and Endocrinology, 2005, 3:5

[24]Gonzales C, Rubio J, Gasco M, Nieto J, Yucra S, Gonzales G F. Effect of short-term and long-term treatments with three ecotypes of Lepidium meyenii (MACA) on spermatogenesis in rats. Journal of Ethnopharmacology, 2006, 103:448-454.

[25]Gonzales G F, Nieto J, Rubio J, Gascoet M. Effect of black maca (Lepidium meyenii) on one spermatogenic cycle in rats. Andrologia, 2006, 38: 166-172.

[26]Clement C, Grados D A D, Avula B, Khan I A, Mayer A C, Aguirre D D P, Manrique I, Kreuzer M. Influence of colour type and previous cultivation on secondary metabolites in hypocotyls and leaves of maca (Lepidiummeyenii Walpers). Journal of the Science of Food and Agriculture, 2010, 90: 861-869.

[27]Brinckmann J, Smith E. Maca culture of the Junín plateau. The Journal of Alternative and Complementary Medicine, 2004, 10(3): 426-430.

[28]刘月萍. 黑芥子酶提取分离、性质及固定化研究[D]. 杭州: 浙江工商大学, 2007.

Liu Y P. The research on the extraction, characterization and immobilization of myrosinase[D]. Hangzhou: Zhejiang Gongshang University, 2007. (in Chinese)

[29]涂行浩, 张 弘, 郑 华, 甘 瑾, 徐珑峰, 李 坤. 响应面法优化微波钝化玛咖黑芥子酶活.食品科学, 2011, 32(18): 148-153.

Tu X H, Zhang H, Zheng H, Gan J, Xu L F, Li K. Optimization of microwave inactivation of myrosinase activity in maca by response surface methodology. Food Science, 2011, 32(18): 148-153. (in Chinese)
[1] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[2] HuiMin HU,XueFeng PAN,Heng YANG,Chen CHEN,YinJi CHEN. Wheat Gluten, Gliadins and Glutenin Content Changes During Germination Based on the Methods of R5 ELISA and RP-HPLC [J]. Scientia Agricultura Sinica, 2020, 53(6): 1247-1255.
[3] LIU QianNan,HUANG Wei,DING YunHua,WANG YaQin,HU LiPing,ZHAO XueZhi,HE HongJu,LIU GuangMin. Rapid Determination of RAA and GBC in Broccoli by Near Infrared Spectroscopy [J]. Scientia Agricultura Sinica, 2020, 53(21): 4497-4506.
[4] XU Ying,YAN ChangYan,YANG WeiCong,ZHANG YunXiao,YU Yang,HUANG XianHui. Pharmacokinetics of Chlortetracycline Microspheres in Pigs [J]. Scientia Agricultura Sinica, 2020, 53(19): 4083-4091.
[5] TIAN ZhiTao, ZHAO YongGuo, LENKA Havlickova, HE Zhesi, ANDREA L Harper, IAN Bancroft, ZOU XiLing, ZHANG XueKun, LU GuangYuan. Dynamic and Associative Transcriptomic Analysis of Glucosinolate Content in Seeds and Silique Walls of Brassica napus [J]. Scientia Agricultura Sinica, 2018, 51(4): 635-651.
[6] LI ZhanSheng, LIU YuMei, FANG ZhiYuan, YANG LiMei, ZHUANG Mu, ZHANG YangYong, Lü HongHao. Full Length Cloning, Expression and Correlation Analysis of P450 CYP79F1 Gene with Sulforaphane Content in Different Broccoli Organs [J]. Scientia Agricultura Sinica, 2018, 51(12): 2357-2367.
[7] ZHANG Yi, WANG Hong-yun, NIU Fu-xiang, SUN Jian, XU Fei, ZHU Hong, YUE Rui-xue. Identification of Purple Sweet Potato Color of Cultivar Ningzi No. 1 by HPLC-QTOF/MS and Its Effect on Preventing Obesity in High-Fat-Diet-Treated Rats [J]. Scientia Agricultura Sinica, 2016, 49(9): 1787-1802.
[8] ZHENG Hui-wen, ZHANG Qiu-yun, LI Wen-hui, ZHANG Shi-kui, XI Wan-peng. Changes in Soluble Sugars and Organic Acids of Xinjiang Apricot During Fruit Development and Ripening [J]. Scientia Agricultura Sinica, 2016, 49(20): 3981-3992.
[9] LIU Shuo, LIU You-chun, LIU Ning, ZHANG Yu-ping, ZHANG Qiu-ping, XU Ming, ZHANG Yu-jun, LIU Wei-sheng. Sugar and Organic Acid Components in Fruits of Plum Cultivar Resources of Genus Prunus [J]. Scientia Agricultura Sinica, 2016, 49(16): 3188-3198.
[10] DU Hai, RAN Feng, LIU Jing, WEN Jing, MA Shan-shan, KE Yun-zhuo, SUN Li-ping, LI Jia-na. Genome-Wide Expression Analysis of Glucosinolate Biosynthetic Genes in Arabidopsis Across Diverse Tissues and Stresses Induction [J]. Scientia Agricultura Sinica, 2016, 49(15): 2879-2897.
[11] LIU Mao-lin, LIU Yi-ming, XU Fei, ZHANG Dao-kang, HUANG Hui-li, LI Han, LI Xiu-bo. The Detection Methods of the Related Substances in Cefquinome Sulfate Intramammary Infusion [J]. Scientia Agricultura Sinica, 2016, 49(15): 3054-3062.
[12] PEI Le, ZHANG Wen-bin, Hasisurong. Determination of the Activities of Bactrian Camel CYP3A Enzyme by Specific Probe Drug [J]. Scientia Agricultura Sinica, 2015, 48(16): 3266-3274.
[13] LIU Jin, ZHANG Ji-fang, LIANG Jian-li, CHENG Feng, WU Jian, WANG Xiao-wu. Evolutionary Study of MAM Genes Which Function in the Process  of Glucosinolates Synthesis in Brassica rapa [J]. Scientia Agricultura Sinica, 2014, 47(21): 4309-4317.
[14] KUANG Min-jie, QI Min-yu, HE Jing-ren, LI Shu-yi, LIU Gang, ZHU Zhen-zhou, CAI Hong-yan, FENG Jun. Identification of Anthocyanins in Brassica campestris L. and Their Stability and Antioxidant Activity [J]. Scientia Agricultura Sinica, 2014, 47(20): 4067-4077.
[15] DING Yan-1, LI Li-Qian-1, CAO Rong-2, TANG Gen-Sheng-2, GU Zhen-Xin-1, HAN Yong-Bin-1. Effect of Enzymolysis Conditions on Glucosinolates in Rapeseed Meal and Identification of Their Degradation Products [J]. Scientia Agricultura Sinica, 2014, 47(2): 383-393.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd