Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (5): 848-853.doi: 10.3864/j.issn.0578-1752.2012.05.004

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Role of Heterotrimeric G Protein in UV-B-Induced Arabidopsis Stomatal Closure

 MA  Xian-Ge, HE  Jun-Min   

  1. 1.陕西师范大学生命科学学院，西安 710062
  • Received:2011-09-26 Online:2012-03-01 Published:2011-12-02

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Abstract: 【Objective】 The role of heterotrimeric G protein in UV-B-induced stomatal closure was analyzed to provide valuable evidence for further elucidating the mechanism of UV-B signaling transduction in plants.【Method】By using Arabidopsis (Arabidopsis thaliana) wild type, Gα-subunit-knockout mutants gpa1-1 and gpa1-2, wGα (a gpa1 overexpression line) and cGα (overexpression constitutively active form mutant of GPA1) combined with pharmacological analysis, the role of heterotrimeric G protein in 0.5 W•m-2 UV-B-induced stomatal closure was explored via stomatal bioassay.【Result】Pharmacological results showed that pertussis toxin (PTX), an inhibitor of G protein α subunit, inhibited UV-B-induced stomatal closure significantly. Meanwhile, cholera toxin (CTX), an activator of G protein α subunit, induced stomatal closure of leaves under light as well as UV-B radiation. Genetic data showed that UV-B-induced stomatal closure was completely impaired in the mutants gpa1-1 and gpa1-2, but not in the overexpression lines of wGα and cGα. In contrast, cGα showed not only smaller stomatal aperture than wild type under light, but also faster stomatal closure induced by UV-B than they did in the wild type.【Conclusion】 Results of this study strongly suggest the involvement of heterotrimeric G protein α subunit in the signaling transduction pathway of UV-B-induced stomatal closure in Arabidopsis.

Key words: heterotrimeric G protein, UV-B radiation, stomatal movement, Arabidopsis

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