拟南芥仅含START结构域亚家族基因特性与功能分析

doi:10.3864/j.issn.0578-1752.2017.17.003

Abstract

Abstract: 【Objective】 The objective of this study is to analyze the promoter elements and expression feature of the genes coding the subfamily containing the START domain (the lipid/sterol-binding StAR-related lipid transfer protein domains) only in Arabidopsis thaliana, expound the relationship between the cis-acting element and the gene function, and the results of study will provide an important theoretical basis for clarifying the biological function of the genes, analyzing the plant growth and development mechanism and promoting or suppressing plant growth better. 【Method】 Using bioinformatics method to analyze the genetic relationship of 6 members and all the elements contained in their respective promoter regions. The function of the promoter elements of subfamily were analyzed and classified, and the expression features and the regulation factors of these subfamily members were predicted. Real-time PCR method was employed to determine the expression characteristics of subfamily members, clarify the relationship between the test results and the conclusions of promoter analysis, thus providing scientific research ideas and direction for analysis of the function and mechanism of genes. The relationship between the gene promoter element and function was confirmed after analysis of the gene mutation type surface. 【Result】 There are 6 members belong to the subfamily containing START domain only in A. thaliana, divided into three branches, every branch has a pair of genes, each pair of genes has a promoter containing high transcription element 5′ UTR Py-rich stretch. The promoter regions of the 6 subfamily members contain multiple common response elements, such as light, hormone and stress, and also have specific response elements for their own. The real-time PCR results of the genes expression level showed that the expression level of At3g13062 was significantly higher than the same branch gene At1g55960, At3g23080 was significantly higher than the same branch gene At4g14500, At1g64720 was significantly higher than the same branch gene At5g54170. All the subfamily members have higher expression levels at seedling stage, but less expression levels at ageing stage, and have its own specific expression of space and time characteristics. In the gene promoter analysis of the family containing START domain structure only, it was found that each gene promoter has a cis-acting element Skn-1 motif which was specially expressed in endosperm, implied that the family genes may be involved in regulation of endosperm development. Seeds analysis of At3g23080 and At4g14500 T-DNA insertion mutant showed that At3g23080 and At4g14500 expression decreased and caused a decline in the seed vigor. 【Conclusion】 There are 6 members in the subfamily containing START domain only. Six members of the family may be play an important role in the response process to light, hormones and stress. the 6 family members have a close kinship, but their expression characteristics of time and space are different and carry out their respective functions in different development periods of A. thaliana. There is a direct relationship between gene promoter elements and the gene function, and the promoter region with endosperm expressed specially element Skn1 motif in At3g23080 and At4g14500, so the decreased expression of At3g23080 or At4g14500 caused a decline in seed vigor.

Key words: Arabidopsis thaliana, START domain, promoter elements; expression feature, gene function

YAN QingDi, ZHAO YaLin, ZHANG Hao, GAO Jing, GAO MengZhu, WANG Qian, WANG Rui, WANG FengRu, DONG JinGao. Analysis on Gene Feature and Function of the Subfamily Members Containing START Domain Only in Arabidopsis thaliana[J].Scientia Agricultura Sinica, 2017, 50(17): 3274-3285.

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Analysis on Gene Feature and Function of the Subfamily Members Containing START Domain Only in Arabidopsis thaliana

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