Abstract:

【Objective】 The objective of this experiment is to explore the mechanism of the compound traditional medicine weeping forsythia regulating immunity by testing the expression of the differentiation signaling transduction protein, calcineurin. 【Method】 To prepare the compound weeping forsythia prescription serum, the Ova and ConA were used as antigen in induction and T cell as presenting cell, the T cells were inlervening cultured for 24 hours, and then the expression of calcineurin was examined by RT- PCR and Western blot. 【Result】 The expression of CaN mRNA was elevated in the 0.1% Ova groups comparing with normal serum and serum-free group. The 0.1% Ova groups were significally different with the 10% Ova groups (P＜0.01). The protein expression of CaN was significantly down regulated in compound weeping forsythia serum groups with Western blot detecting, but there was no significant difference between the two groups at concentration of 0.1% Ova. The CaN expression of the compound weeping forsythia serum group was elevated obviously compared with the group without serum (P＜0.05). 【Conclusion】The compound weeping forsythia has provided an immunomodulation by transduction of regulatory signal, and by realizing many aspects and double direction regulation. The results showed that the compound weeping forsythia has more referential values of the selection medication on deficiency syndrome and excess syndrome.

Key words: the compound weeping forsythia, Th differentiation, signal transduction, calcineurin