Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (12): 2209-2219.doi: 10.3864/j.issn.0578-1752.2017.12.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transcription Abundances of Two Phytochrome C in Response to Different Light Treatments in Zea mays

NIU Xiang 1,2, GUO Lin 2, YANG ZongJu 2,3, SUN Lei 2,3, LI HongDan 2,3, YOU GuangXia 2, XU Hong 4, MENG FanHua 2, SHE YueHui 1, YANGJianPing 2   

  1. 1 College of Agronomy, Sichuan Agricultural University, Chengdu 611130; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081; 3 Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081; 4 Huangchuan Institute of Agricultural Sciences of Henan Province, Huangchuan 465150, Henan
  • Received:2016-12-16 Online:2017-06-16 Published:2017-06-16

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Abstract: 【Objective】To study the functions of phytochrome C genes in seedling de-etiolation and flowering regulation in maize (Zea mays L.), two phytochrome C genes of maize (ZmPHYC1 and ZmPHYC2) were selected from the NCBI database and analyzed by bioinformatic methods. The transcription abundances of two ZmPHYC genes was analyzed in different tissues and in response to light qualities, transitions from the dark to different light conditions, photoperiod treatment (long day and short day) by quantitative RT-PCR (qRT-PCR).【Method】B73 inbred line was used in this study, the full length ORFs of two ZmPHYC genes were cloned by RT-PCR. The proper clones were sequenced and analyzed by bioinformatics software. The transcription abundances of two ZmPHYC genes in different tissues and in response to light treatments was detected using qRT-PCR.【Result】Both the full length ORFs of ZmPHYC1 and ZmPHYC2 contained 3408 nucleotides and encoded two polypeptides with 1135 amino acid motifs, and their molecular weight was 126.14 kD and 126.07 kD, respectively. Two ZmphyC proteins were able to be further divided into six domains: Per (period circadian protein)-Arnt (Ah receptor nuclear translocator protein)-Sim (single-minded protein) (PAS), cGMP-stimulated phosphodiesterase (GAF), phytochrome (PHY), PAS-related domain (PRD) containing two PAS, His Kinase A domain (HisKA), Histidine kinase-like ATPases (HATPase_c), while ZmphyC2 lacked a PAS in PRD domain. Phylogenetic analysis indicated that the two ZmphyC proteins belonged to the same branch with phyC proteins from other graminaceous species, especially with the phyC proteins from sugarcane and sorghum. qRT-PCR assays showed that both ZmPHYC1 and ZmPHYC2 belonged to tissue-specific genes and highly expressed in roots and leaves. The transcription abundances of the both genes were very high under blue and white light conditions. Both ZmPHYC1 and ZmPHYC2 displayed similar expression patterns during transitions from the dark to different light conditions. The transcription abundances of the both genes went dramatically up at 0.5 h after transitions from the dark to far-red, red, blue, or white light. And then they quickly dropped and waved below their own levels in the dark. Both ZmPHYC1 and ZmPHYC2 were also able to respond to long-day or short-day treatments. During long-day treatment, they likely had one peak in either light or dark period. During short-day treatment, they showed different expression patterns, the peak of ZmPHYC1 happened at 6 h after conversion to dark period, while ZmPHYC2 occurred at 2 h after conversion to light period.【Conclusion】 ZmphyC1 protein kept six domains, while ZmphyC2 lacks a PAS in PRD domain. The transcription abundances of the both PHYC genes were tissue-specific in maize. Similar expression patterns of ZmPHYC1 and ZmPHYC2 genes in response to different light treatments suggest that they both might keep redundant functions. Since the transcription abundances of ZmphyC1 were higher than these of ZmphyC2, ZmphyC1 might have more important role in seedling responding to light than ZmphyC2, which may be due to the existence of different functions in maize. Both ZmPHYC1 and ZmPHYC2 respond to light and photoperiod treatments, suggesting that they are involved in seedling de-etiolation and flowering time control in maize. Thus their roles in crop improvement are worthy of more exploration in the future.

Key words: Zea mays, ZmPHYC, light signal transduction, expression pattern, light treatment

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