Abstract:

【Objective】 To study the expression profiles of genes related to signal transduction in the mature wheat embryo dedifferentiation to elucidate the molecular mechanism of dedifferentiation. 【Method】 Expression profile of genes in dedifferentiation was studied using Affymetrix microarray technique in mature embryos of wheat cultivar Yumai 18 cultured on MS medium supplemented with 2 mg•L-1 of 2, 4-dichlorophenolxyacetic acid (2, 4-D) at different time points. Their expression information was processed using online tools at NCBI, DATF, and DRTF websites. The expression changes of 13 genes, i.e., AF161719.1, BE492852, BJ218430, BJ252470, BJ272518, BJ274015, CA616149, CD896892, CK153462, CK207725, CK171662. CD901339, and U48692.1, were varified using semi- quantative RT-PCR technique. 【Result】 Forty-six genes related to signal transduction changed at least at one time point were detected including 32 up-regulated and 14 down-regulated genes during the whole period. They were calcium, serine/threonine protein kinase, casein kinase, protein kinase, 14-3-3 protein, mitogen activated protein kinase. Expression of some genes were confirmed to be consistant with the results of affymetrix microarray. 【Conclusion】 CA613597, CA674315, BJ274015, CA654806, CD901339, CK198900, CA697195, CA642143, CA744550, and AB011670.1 might play an important role in triggering and promoting dedifferentiation of mature embryo in wheat.

Key words: genes related to signal transduction, dedifferentiation of mature wheat embryo, microarray