水通道蛋白基因OsPIP2;6的功能分析

doi:10.3864/j.issn.0578-1752.2013.15.001

摘要/Abstract

摘要： 【目的】分析水稻过表达OsPIP2;6在逆境胁迫条件下的表型差异，探讨水通道蛋白基因在水稻逆境应答中的作用。【方法】构建水稻水通道蛋白OsPIP2;6的过表达载体，通过农杆菌介导将其导入水稻日本晴的愈伤中，经遗传转化，阳性鉴定，得到转基因植株。通过逆境胁迫下转基因植株的表型分析来探讨OsPIP2;6的功能。【结果】Real-time PCR结果显示，OsPIP2;6受GA、ABA调控。转基因植株的OsPIP2;6表达量显著提高。正常条件下，转基因植株与野生型植株生长发育情况并无显著差异。在逆境胁迫条件下，转基因植株的耐受能力均显著强于野生型植株。【结论】过表达水稻水通道蛋白基因OsPIP2;6的转基因植株表型显示，OsPIP2;6在水稻的抗旱、抗水淹和抗盐中发挥作用。

关键词: 水稻 , 水通道蛋白 , 干旱 , 盐胁迫

Abstract: 【Objective】 The current study aims to investigate the functional significance of a rice aquaporin (OsPIP2;6) by analyzing the phenotypic alteration resulting from overexpressing OsPIP2;6 in responses to stressed conditions.【Method】The overexpression vector of 35S::pHB-OsPIP2;6 was constructed and introduced into rice cv. Nipponbare by Agrobacterium-mediated transformation .The phenotypic analysis and molecular detection of transgenic plants were performed to verify the function of OsPIP2;6. 【Result】 Real-time PCR indicated that OsPIP2;6 was regulated by gibberellin (GA), abscisic acid (ABA) and the expression levels of OsPIP2;6 in transgenic lines were significantly increased. There was no obvious difference in growth between transgenic plants and wild type under normal condition. The performance of T2 transgenic seedlings revealed that OsPIP2;6 overexpression lines showed increased growth rates under stresses condition. 【Conclusion】 The phenotype of overexpressing OsPIP2;6 lines suggested a role of OsPIP2;6 in resistance to draught, waterlogging, and salt stress.

Key words: Oryza sativa L. , aquaporin , draught , salt stress

李嵘1, 牛向丽2, 苗雁文1, 熊方杰1, 刘永胜12. 水通道蛋白基因OsPIP2;6的功能分析[J]. 中国农业科学, 2013, 46(15): 3079-3086.

LI Rong-1, NIU Xiang-Li-2, MIAO Yan-Wen-1, XIONG Fang-Jie-1, LIU Yong-Sheng-12. Functional Characterization of the Plasma Intrinsic Protein Gene OsPIP2;6 in Rice[J]. Scientia Agricultura Sinica, 2013, 46(15): 3079-3086.

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