中国农业科学 ›› 2013, Vol. 46 ›› Issue (15): 3079-3086.doi: 10.3864/j.issn.0578-1752.2013.15.001

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

水通道蛋白基因OsPIP2;6的功能分析

 李嵘1, 牛向丽2, 苗雁文1, 熊方杰1, 刘永胜12   

  1. 1.重庆大学生命科学学院,重庆 400030 
    2.合肥工业大学生物与食品工程学院,合肥 230009
  • 收稿日期:2013-03-11 出版日期:2013-08-01 发布日期:2013-05-22
  • 通讯作者: 通信作者刘永胜,Tel:18709832886;E-mail:liuyongsheng1122@hfut.edu.cn
  • 作者简介:李嵘,Tel:15908149525;E-mail:lr429@sina.com
  • 基金资助:

    重庆市科技攻关项目(2010AA1019)

Functional Characterization of the Plasma Intrinsic Protein Gene OsPIP2;6 in Rice

 LI  Rong-1, NIU  Xiang-Li-2, MIAO  Yan-Wen-1, XIONG  Fang-Jie-1, LIU  Yong-Sheng-12   

  1. 1.School of Life Sciences, Chongqing University, Chongqing 400030
    2.School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009
  • Received:2013-03-11 Online:2013-08-01 Published:2013-05-22

摘要: 【目的】分析水稻过表达OsPIP2;6在逆境胁迫条件下的表型差异,探讨水通道蛋白基因在水稻逆境应答中的作用。【方法】构建水稻水通道蛋白OsPIP2;6的过表达载体,通过农杆菌介导将其导入水稻日本晴的愈伤中,经遗传转化,阳性鉴定,得到转基因植株。通过逆境胁迫下转基因植株的表型分析来探讨OsPIP2;6的功能。【结果】Real-time PCR结果显示,OsPIP2;6受GA、ABA调控。转基因植株的OsPIP2;6表达量显著提高。正常条件下,转基因植株与野生型植株生长发育情况并无显著差异。在逆境胁迫条件下,转基因植株的耐受能力均显著强于野生型植株。【结论】过表达水稻水通道蛋白基因OsPIP2;6的转基因植株表型显示,OsPIP2;6在水稻的抗旱、抗水淹和抗盐中发挥作用。

关键词: 水稻 , 水通道蛋白 , 干旱 , 盐胁迫

Abstract: 【Objective】 The current study aims to investigate the functional significance of a rice aquaporin (OsPIP2;6) by analyzing the phenotypic alteration resulting from overexpressing OsPIP2;6 in responses to stressed conditions.【Method】The overexpression vector of 35S::pHB-OsPIP2;6 was constructed and introduced into rice cv. Nipponbare by Agrobacterium-mediated transformation .The phenotypic analysis and molecular detection of transgenic plants were performed to verify the function of OsPIP2;6. 【Result】 Real-time PCR indicated that OsPIP2;6 was regulated by gibberellin (GA), abscisic acid (ABA) and the expression levels of OsPIP2;6 in transgenic lines were significantly increased. There was no obvious difference in growth between transgenic plants and wild type under normal condition. The performance of T2 transgenic seedlings revealed that OsPIP2;6 overexpression lines showed increased growth rates under stresses condition. 【Conclusion】 The phenotype of overexpressing OsPIP2;6 lines suggested a role of OsPIP2;6 in resistance to draught, waterlogging, and salt stress.

Key words: Oryza sativa L. , aquaporin , draught , salt stress