乙烯与赤霉素协同调控水稻胚芽鞘伸长生长

doi:10.3864/j.issn.0578-1752.2026.16.004

摘要/Abstract

摘要：

【目的】乙烯和赤霉素是调控水稻胚芽鞘伸长的关键激素，但二者在该过程中的交互作用机制尚不明确，本文旨在探究二者在调控胚芽鞘伸长中的分子机制。【方法】利用乙烯和赤霉素信号通路核心组分的相关水稻材料，统计不同激素处理后胚芽鞘的长度，采用qPCR技术检测OsEIN2/OsEIL2下游基因表达，利用酵母双杂和Pull-down技术验证OsSLR1与OsEIL2互作。【结果】乙烯和赤霉素均能促进胚芽鞘的伸长，且二者存在协同效应。赤霉素合成抑制剂多效唑（Paclobutrazol，PAC）处理或过表达赤霉素失活基因OsGA2ox3削弱了乙烯对胚芽鞘伸长的促进作用，外源补充赤霉素也能够部分解除1-MCP（1-Methylcyclopropene）对胚芽鞘的抑制，表明乙烯与赤霉素调控胚芽鞘的伸长存在共同的调控途径。进一步研究发现，赤霉素信号负调控因子OsSLR1与OsEIL2存在相互作用，且OsEIN2/OsEIL2调控的下游基因OsERF63、OsERF73、OsHKT2;1和OsGY1也受到了OsSLR1的调控，OsSLR1功能缺失突变体slr1表现出长胚芽鞘的表型，且乙烯对胚芽鞘伸长的促进作用在slr1中进一步增强，表明OsSLR1参与调控乙烯促进的胚芽鞘生长和信号转导。【结论】 OsSLR1是乙烯和赤霉素协同调控胚芽鞘伸长生长的节点，通过与OsEIL2互作来共同调控下游信号通路和胚芽鞘的伸长生长，这不仅丰富了胚芽鞘生长中的植物激素互作网络，也为直播稻新品种的培育提供了理论依据和有用的基因。

关键词: 水稻, 胚芽鞘, 乙烯, 赤霉素, OsEIL2, OsSLR1

Abstract:

【Objective】 Ethylene and gibberellin are key phytohormones regulating rice coleoptile elongation, however, the interaction of ethylene and gibberellin in coleoptile elongation remains unclear. This study aimed to investigate the molecular mechanism of ethylene and gibberellin in regulating coleoptile elongation.【Method】 This study utilized transgenic rice lines of the core components of ethylene and gibberellin signaling pathways as experimental materials. The coleoptile lengths were examined with or without different phytohormone treatments. The expression levels of downstream genes of OsEIN2/OsEIL2 were detected by RT-qPCR. Yeast two-hybrid and pull-down assays were further employed to verify the interaction between OsSLR1 and OsEIL2. 【Result】 Exogenous phytohormones treatment revealed that both ethylene and gibberellin promoted coleoptile elongation, and they exhibited a synergistic effect. Paclobutrazol (PAC, a GA biosynthesis inhibitor) treatment or overexpression of the gibberellin inactivation gene OsGA2ox3 weakened the promoting effect of ethylene on coleoptile elongation, whereas exogenous application of gibberellin partially relieved the 1-MCP (1-Methylcyclopropene) inhibited coleoptile elongation, indicating that ethylene and gibberellin shared a common regulatory pathway in controlling coleoptile elongation. Further studies revealed that OsSLR1, a negative regulator of gibberellin signaling, interacted with OsEIL2. Moreover, OsEIN2/OsEIL2-regulated genes, such as OsERF63, OsERF73, OsHKT2; 1 and OsGY1 were also regulated by OsSLR1. The OsSLR1 loss-of-function mutant slr1 exhibited a long coleoptile phenotype, and the promoting effect of ethylene on coleoptile elongation was further enhanced in slr1, suggesting that OsSLR1 was involved in ethylene-mediated coleoptile elongation and signal transduction. 【Conclusion】 In summary, this study revealed that OsSLR1 served as a crosstalk node for the synergistic regulation of coleoptile elongation by ethylene and gibberellin, and it interacted with OsEIL2 to co-regulate downstream signaling pathways and coleoptile elongation. This study not only enriched the understanding of the phytohormone interaction network in coleoptile growth, but also provided a theoretical basis and useful genes for the breeding of rice varieties suitable for direct seeding cultivation.

Key words: rice, coleoptile, ethylene, gibberellin, OsEIL2, OsSLR1

吴斌, 葛秉坤, 秦田雨, 肖桂青, 秦华. 乙烯与赤霉素协同调控水稻胚芽鞘伸长生长[J]. 中国农业科学, 2026, 59(16): 3509-3518.

WU Bin, GE BingKun, QIN TianYu, XIAO GuiQing, QIN Hua. Ethylene and Gibberellin Synergistically Regulate Coleoptile Elongation in Rice[J]. Scientia Agricultura Sinica, 2026, 59(16): 3509-3518.

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