铁在内生放线菌OsiPR-1诱导水稻稻瘟病抗性中的作用

doi:10.3864/j.issn.0578-1752.2026.13.006

中国农业科学 ›› 2026, Vol. 59 ›› Issue (13): 2828-2840.doi: 10.3864/j.issn.0578-1752.2026.13.006

铁在内生放线菌OsiPR-1诱导水稻稻瘟病抗性中的作用

赵星宇¹(), 高艳¹^,²(), 唐新科¹^,²(), 奉茵陈¹, 陈思颖¹^,², 顾天宇¹^,², 彭佳师¹^,²

¹ 湖南科技大学生命科学与健康学院，湖南湘潭 411201
² 经济作物遗传改良与综合利用湖南省重点实验室/重金属污染土壤生态修复与安全利用湖南省高校重点实验室，湖南湘潭 411201

收稿日期:2026-03-05 接受日期:2026-04-21 出版日期:2026-07-01 发布日期:2026-07-01
通信作者:
高艳，E-mail：blessedgy@hnust.edu.cn
唐新科，E-mail：1090031@hnust.edu.cn
联系方式: 赵星宇，E-mail：2812184448@qq.com。
基金资助:
湖南省自然科学基金(2026JJ50149); 湖南省教育厅项目(24A0351); 湖南省“三尖”创新人才工程(2023RC3177); 经济作物遗传改良与综合利用湖南省重点实验室开放基金项目(E22437); 经济作物遗传改良与综合利用湖南省重点实验室开放基金项目(E22532)

The Role of Iron in Enhancing Rice Blast Resistance Induced by Endophytic Streptomyces hyaluromycini OsiPR-1

ZHAO XingYu¹(), GAO Yan¹^,²(), TANG XinKe¹^,²(), FENG YinChen¹, CHEN SiYing¹^,², GU TianYu¹^,², PENG JiaShi¹^,²

¹ School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, Hunan
² Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization/Hunan Province University Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, Xiangtan 411201, Hunan

Received:2026-03-05 Accepted:2026-04-21 Published:2026-07-01 Online:2026-07-01

摘要/Abstract

摘要：

【目的】铁广泛参与植物生长发育和免疫应答等重要生命活动。内生菌定殖于植物体内已进化出高效的铁摄取和稳态调节机制，影响宿主抗病性。挖掘水稻内生菌资源并探究铁营养对其诱导宿主抗病性的影响，以期为植物病害绿色防控提供理论支撑。【方法】采用病叶表面消毒结合平板划线法分离水稻内生菌；通过平板对峙法筛选对稻瘟病菌（Magnaporthe oryzae）具有拮抗活性的菌株；结合形态学观察及基于16S rRNA等多基因序列的系统发育分析，对目标菌株进行鉴定；利用CAS检测培养基和铁载体合成相关基因扩增法分析目标菌株产铁载体能力；设置正常、缺铁和高铁3种培养环境，测定喷施目标菌株后水稻叶片活性氧迸发、铁积累水平和铁代谢相关基因的表达水平，并评估不同铁营养条件下内生菌诱导水稻对稻瘟病的抗性。【结果】从水稻体内分离获得一株对稻瘟病菌具有稳定拮抗活性的内生放线菌OsiPR-1，鉴定为透明质酸链霉菌（Streptomyces hyaluromycini）。该菌株具有较强的产铁载体能力，在缺铁培养条件下第6天铁载体产量达峰值（60.2%），且基因组中存在3个铁载体合成基因簇。回接试验表明，该菌株的定殖能力及诱导抗病性与环境铁营养水平密切相关。在正常铁浓度下，OsiPR-1诱导水稻早期气孔中铁与活性氧积累以激发免疫响应，并通过分泌铁载体调控铁稳态恢复以建立稳定共生关系，使稻瘟病病斑长度显著降低74.8%。高铁条件下，OsiPR-1诱导的铁与活性氧仅在处理4 h时短暂出现于叶脉，随后迅速消失，菌株虽大量增殖但共生关系不稳定，病斑长度仅降低64.5%。缺铁条件下，气孔中铁与活性氧积累微弱，但铁转运（OsIRT1）、铁稳态（OsFRO2）及脂质过氧化（OsLOX2）相关基因表达显著上调（7.7—10.3倍），铁稳态剧烈波动及过度免疫反应抑制内生菌定殖，共生难以建立，抗病效果大幅下降。【结论】正常铁供给是透明质酸链霉菌OsiPR-1通过铁载体介导的免疫诱导机制增强水稻稻瘟病抗性的关键前提。研究结果为开发基于铁营养精准调控的稻瘟病高效生防菌剂提供了理论依据，并为深入理解植物-内生菌互作中的铁营养机制提供了新视角。

关键词: 水稻, 稻瘟病, 稻瘟病菌, 内生菌, 铁, 铁载体, 生物防治

Abstract:

【Objective】 Iron (Fe) is widely involved in critical life processes such as plant growth, development, and immune responses. Endophytes colonizing plant tissues have evolved efficient mechanisms for Fe uptake and homeostasis regulation, thereby positively influencing host disease resistance. This study aims to explore rice endophytic resources and investigate the impact of Fe nutrition on their ability to induce host resistance, and to provide a theoretical basis for green management of plant diseases.【Method】Endophytic bacteria were isolated from rice leaves using surface disinfection combined with the streak plate method. Target strains with antagonistic activity against Magnaporthe oryzae were screened using the dual culture method. The selected strain was identified through morphological observation and phylogenetic analysis based on 16S rRNA and multi-gene sequences. Its siderophore-producing ability was analyzed using CAS assay medium and by amplifying genes related to siderophore biosynthesis. Three culture conditions (normal Fe, Fe-deficient, and high Fe) were established to assess the reactive oxygen species (ROS) burst, Fe accumulation, and expression levels of Fe metabolism-related genes in rice leaves after spraying with the target strain. The efficacy of the endophyte in inducing rice resistance to rice blast under different Fe regimes was evaluated.【Result】An endophytic actinomycete strain, OsiPR-1, with stable antagonistic activity against M. oryzae, was isolated from rice and identified as Streptomyces hyaluromycini. This strain exhibited a strong ability to produce siderophores, with peak production (60.2%) observed on the 6th day under Fe-deficient conditions, and three siderophore biosynthesis gene clusters were detected in its genome. Reinoculation tests showed that the strain’s colonization and its induction of blast resistance were closely related to environmental Fe levels. Under normal Fe conditions, OsiPR-1 induced early accumulation of Fe and ROS in rice stomata to trigger immune responses, and subsequently regulated Fe homeostasis restoration via secreted siderophores to establish a stable symbiotic relationship, significantly reducing rice blast lesion length by 74.8%. Under high Fe conditions, Fe and ROS accumulation induced by OsiPR-1 appeared transiently in leaf veins only at 4 h post-treatment and then rapidly disappeared. Although the strain proliferated substantially, the symbiotic relationship was unstable, resulting in a lesion length reduction of only 64.5%. Under Fe-deficient conditions, Fe and ROS accumulation in stomata was weak. However, genes involved in Fe transport (OsIRT1), Fe homeostasis (OsFRO2), and lipoxygenase catalysis (OsLOX2) were significantly up-regulated (7.7-10.3 folds). The drastic fluctuation in Fe homeostasis and the subsequent intense immune response inhibited endophyte colonization, hindering the establishment of symbiosis and leading to a sharp decline in disease control efficacy.【Conclusion】Normal Fe supply is a critical prerequisite for S. hyaluromycini OsiPR-1 to enhance rice resistance against blast through a siderophore-mediated immune induction mechanism. This study provides an important theoretical foundation for developing efficient biocontrol agents against rice blast based on precise regulation of Fe nutrition and offers new insights into the role of Fe nutrition in plant-endophyte interactions.

Key words: rice, rice blast, Magnaporthe oryzae, endophyte, iron, siderophore, biological control

赵星宇, 高艳, 唐新科, 奉茵陈, 陈思颖, 顾天宇, 彭佳师. 铁在内生放线菌OsiPR-1诱导水稻稻瘟病抗性中的作用[J]. 中国农业科学, 2026, 59(13): 2828-2840.

ZHAO XingYu, GAO Yan, TANG XinKe, FENG YinChen, CHEN SiYing, GU TianYu, PENG JiaShi. The Role of Iron in Enhancing Rice Blast Resistance Induced by Endophytic Streptomyces hyaluromycini OsiPR-1[J]. Scientia Agricultura Sinica, 2026, 59(13): 2828-2840.

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引物名称 Primer name	引物序列 Primer sequence (5′-3′)	引物名称 Primer name	引物序列 Primer sequence (5′-3′)
OsUbq-F	TTCTGGTCCTTCCACTTTCAG	rpoB-F	GCTGATCCAGAACCAGGTCC
OsUbq-R	ACGATTGATTTAACCAGTCCATGA	rpoB-R	CGGGTTGTTCTGGTCCATGA
OsIRT1-F	GCAATTCGCTGCATTGTTAG	Gene of siderophore 1-F	GCTACTACTTCCGCGAGTCC
OsIRT1-R	GAAGTACATCATCAGTCACGAA	Gene of siderophore 1-R	CCGAGGTAGTAGGCGAAACG
OsFRO2-F	GATCCATGTCAAGCCTGTCGA	Gene of siderophore 2-F	TCGAACCCCATCTGCAGAAC
OsFRO2-R	AGGGACGAGATCGTCTCGTACA	Gene of siderophore 2-R	GGTTGACCAGGAGGCAGTAC
OsLOX2-F	AGATGAGGCGCGTGATGAC	Gene of siderophore 3-F	CATCCCTTCCACCCCAACTG
OsLOX2-R	CATGGAAGTCGAGCATGAACA	Gene of siderophore 3-R	GGATGCACCGGGATCAACA

铁在内生放线菌OsiPR-1诱导水稻稻瘟病抗性中的作用

The Role of Iron in Enhancing Rice Blast Resistance Induced by Endophytic Streptomyces hyaluromycini OsiPR-1

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