中国农业科学 ›› 2019, Vol. 52 ›› Issue (11): 1961-1969.doi: 10.3864/j.issn.0578-1752.2019.11.010
李辛雷,殷恒福,范正琪,李纪元
收稿日期:
2018-12-29
接受日期:
2019-03-05
出版日期:
2019-06-01
发布日期:
2019-06-11
作者简介:
李辛雷,Tel:15968855095;E-mail:lixinlei2020@163.com
基金资助:
LI XinLei,YIN HengFu,FAN ZhengQi,LI JiYuan
Received:
2018-12-29
Accepted:
2019-03-05
Online:
2019-06-01
Published:
2019-06-11
摘要:
目的 研究山茶芽变花色与花青苷的关系,为山茶花色的芽变育种提供科学依据。方法 按照CIE L* a* b*表色系法测量山茶及其芽变品种的花色,利用高效液相色谱-光电二极管阵列检测(HPLC-DAD)和超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)联用技术定性定量分析其花瓣中花青苷成分与含量,运用多元线性回归方法研究花青苷与花色之间的关系。结果 山茶及其芽变品种花瓣中共检测到7种花青苷,分别是矢车菊素-3-O-β-半乳糖苷(Cy3Ga)、矢车菊素-3-O-β-葡萄糖苷(Cy3G)、矢车菊素-3-O-[6-O-(E)-咖啡酰]-β-半乳糖苷(Cy3GaECaf)、矢车菊素-3-O-[6-O-(E)-咖啡酰]-β-葡萄糖苷(Cy3GECaf)、矢车菊素-3-O-[6-O-(Z)-p-香豆酰]-β-葡萄糖苷(Cy3GZpC)、矢车菊素-3-O-[6-O-(E)-p-香豆酰]-β-半乳糖苷(Cy3GaEpC)和矢车菊素-3-O-[6-O-(E)-p-香豆酰]-β-葡萄糖苷(Cy3GEpC)。山茶各系列芽变品种中,白色花瓣中均未检测到花青苷,红色花瓣中花青苷成分与粉色花瓣相同,但红色花瓣中各成分含量及花青苷总量均远高于粉色花瓣;红色和粉色花瓣中主要花青苷成分为Cy3G和Cy3GEpC;红色花瓣中Cy3G和Cy3Ga所占比例大于粉色花瓣,而Cy3GEpC等花青苷比例小于粉色花瓣。结论 山茶各系列芽变品种中各种花青苷含量及花青苷总量越大,花瓣红色越深;Cy3G、Cy3Ga和Cy3GEpC是决定山茶芽变花色的主要花青苷成分,其含量的积累增加花瓣红色程度。
李辛雷,殷恒福,范正琪,李纪元. 山茶芽变花色与花青苷的关系[J]. 中国农业科学, 2019, 52(11): 1961-1969.
LI XinLei,YIN HengFu,FAN ZhengQi,LI JiYuan. The Relationship Between Anthocyanins and Flower Colors of Bud Mutation in Camellia japonica [J]. Scientia Agricultura Sinica, 2019, 52(11): 1961-1969.
表1
山茶品种花色数据"
品种 Cultivars | CIE L*a*b*表色系统 CIE L*a*b*coordinate | |||||
---|---|---|---|---|---|---|
花色 Flower color | L* | a* | b* | C* | h | |
白宝珠Baibaozhu | 白色 White | 93.09±1.23 | -0.70±0.02 | 1.65±0.38 | 1.80 ±0.25 | 92.72±2.01 |
白芙蓉Baifurong | 白色 White | 89.10±2.01 | -0.51±0.01 | 3.21±0.83 | 3.27±0.35 | 101.21±1.09 |
白嫦娥彩Baichangecai | 白色 White | 88.21±1.57 | -0.93±0.12 | 4.77±0.47 | 4.86±1.21 | 101.19±1.37 |
白五宝Baiwubao | 白色 White | 90.13±1.46 | -0.71±0.05 | 3.21±0.53 | 3.31±0.23 | 105.04±3.11 |
白碧玉Baibiyu | 白色 White | 87.57±1.82 | -0.59±0.05 | 6.53±1.18 | 6.57±0.51 | 95.22± 2.25 |
白七仙女Baiqixiannv | 白色 White | 88.65± 3.12 | -0.68±0.06 | 4.46±0.45 | 4.64±0.29 | 98.19±1.85 |
平均 Average | 89.46 | -0.69 | 3.97 | 4.07 | 98.93 | |
粉宝珠Fenbaozhu | 粉色Pink | 65.90±1.23 | 39.90±0.86 | 2.93±0.25 | 40.03±1.38 | 4.11±1.22 |
粉芙蓉Fenfurong | 粉色Pink | 69.75±0.95 | 36.95±1.56 | 9.87±1.08 | 37.00± 1.26 | 6.82±2.05 |
粉嫦娥彩Fenchangecai | 粉色Pink | 67.62±1.26 | 41.71±1.87 | 9.94±0.74 | 33.81±2.18 | 3.72± 0.96 |
粉五宝Fenwubao | 粉色Pink | 83.87±1.85 | 31.73±1.35 | 8.62±0.69 | 37.23± 1.56 | 7.68±1.63 |
粉碧玉Fenbiyu | 粉色Pink | 71.06±1.63 | 35.57±1.65 | 2.43±0.15 | 35.56± 1.29 | 3.90±0.28 |
粉七仙女Fenqixiannv | 粉色Pink | 76.77±2.14 | 32.19±1.34 | 7.17±0.42 | 26.89± 1.18 | 6.37±1.45 |
平均 Average | 72.49 | 36.34 | 6.83 | 35.09 | 5.43 | |
红宝珠Hongbaozhu | 红色 Red | 54.08±2.15 | 55.91±2.24 | 20.22±1.65 | 57.07±2.31 | 21.24±1.92 |
红芙蓉Hongfurong | 红色 Red | 54.99±1.43 | 52.70±1.78 | 18.48±2.46 | 53.39±1.54 | 19.14±2.26 |
红嫦娥彩Hongchangecai | 红色 Red | 50.62±1.28 | 48.71±1.53 | 21.94±1.09 | 53.81±1.66 | 23.72±1.88 |
红五宝Hongwubao | 红色 Red | 56.00±3.26 | 50.54±1.64 | 15.50± 1.16 | 50.85±1.25 | 16.16±1.25 |
红碧玉Hongbiyu | 红色 Red | 62.18±1.27 | 55.44±2.13 | 13.85±0.87 | 45.61±1.29 | 14.80±1.36 |
红七仙女Hongqixiannv | 红色 Red | 62.33±1.88 | 49.35±1.39 | 18.66±1.91 | 49.42±1.16 | 16.76±1.11 |
平均 Average | 56.70 | 52.11 | 18.11 | 51.69 | 18.64 |
表2
山茶花青苷成分的紫外-可见吸收光谱与质谱数据"
色谱峰 No.Peaks | 保留时间 Retention time (min) | 吸收波长 λmax (nm) | A440/Avis-max (%) | 分子离子 Molecular ions (m/z) | 碎片离子 Fragment ions (m/z) | 推定结果 Tentative identification |
---|---|---|---|---|---|---|
P1 | 8.33 | 281, 516 | 31 | 449 | 287 | Cy3Ga |
P2 | 9.43 | 282, 517 | 32 | 449 | 287 | Cy3G |
P3 | 21.22 | 283, 315, 516 | 33 | 611 | 449, 287 | Cy3GaECaf |
P4 | 24.75 | 283, 316, 515 | 33 | 611 | 449, 287 | Cy3GECaf |
P5 | 25.54 | 282, 311, 514 | 32 | 595 | 449, 287 | Gy3GZpC |
P6 | 25.95 | 284, 312, 516 | 32 | 595 | 449, 287 | Cy3GaEpC |
P7 | 29.74 | 285, 312, 513 | 32 | 595 | 449, 287 | Cy3GEpC |
表3
不同山茶品种花青苷成分含量"
品种 Cultivars | 花青苷 Anthocyanin (μg·100 mg-1) | |||||||
---|---|---|---|---|---|---|---|---|
Cy3Ga | Cy3G | Cy3GaECaf | Cy3GECaf | Gy3GZpC | Cy3GaEpC | Cy3GEpC | 合计 Total | |
白宝珠Baibaozhu | - | - | - | - | - | - | - | - |
粉宝珠Fenbaozhu | 1.23±0.13 | 8.91±0.47 | 0.25±0.12 | 0.16±0.00 | 0.59±0.04 | 0.98±0.42 | 5.12±0.23 | 17.24 |
红宝珠Hongbaozhu | 7.42±0.35 | 62.32±0.83 | 1.12±0.03 | 0.65±0.01 | 1.44±0.00 | 5.14±0.16 | 13.58±0.26 | 91.67 |
白芙蓉Baifurong | - | - | - | - | - | - | - | - |
粉芙蓉Fenfurong | 1.30±0.04 | 9.16±0.35 | 0.34±0.00 | 0.28±0.00 | 0.91±0.17 | 0.68±0.01 | 3.37±0.11 | 16.04 |
红芙蓉Hongfurong | 5.89±0.33 | 45.91±0.78 | 0.77±0.02 | 0.45±0.06 | 2.04±0.05 | 2.54±0.15 | 9.86±0.67 | 67.46 |
白嫦娥彩Baichangecai | - | - | - | - | - | - | - | - |
粉嫦娥彩Fenchangecai | 1.12±0.02 | 3.12±0.05 | - | - | 0.76±0.00 | 0.56±0.01 | 2.82±0.51 | 8.38 |
红嫦娥彩Hongchangecai | 5.71±0.05 | 18.72±0.45 | - | - | 1.91±0.12 | 2.42±0.08 | 8.19±0.71 | 36.95 |
白五宝Baiwubao | - | - | - | - | - | - | - | - |
粉五宝Fenwubao | 0.11±0.00 | 5.25±0.19 | - | 0.18±0.00 | 2.27±0.15 | 0.64±0.07 | 15.75±0.43 | 24.2 |
红五宝Hongwubao | 0.42±0.05 | 14.52±0.35 | - | 0.56±0.07 | 4.16±0.32 | 1.08±0.01 | 27.42±0.37 | 48.16 |
白碧玉Baibiyu | - | - | - | - | - | - | - | - |
粉碧玉Fenbiyu | 0.12±0.00 | 2.07±0.05 | - | - | 0.83±0.01 | 0.23±0.00 | 2.21±0.28 | 5.46 |
红碧玉Hongbiyu | 0.71±0.06 | 7.86±0.33 | - | - | 2.08±0.05 | 0.82±0.17 | 8.56±0.45 | 20.03 |
白七仙女Baiqixiannv | - | - | - | - | - | - | - | - |
粉七仙女Fenqixiannv | 0.16±0.00 | 2.23±0.18 | - | - | 0.51±0.13 | 0.25±0.01 | 2.71±0.23 | 5.86 |
红七仙女Hongqixiannv | 0.44±0.01 | 4.74±0.18 | - | - | 0.63±0.00 | 0.39±0.01 | 5.16±0.16 | 11.36 |
表4
不同山茶品种花青苷成分比例"
品种 Cultivars | 花青苷 Anthocyanin (%) | |||||||
---|---|---|---|---|---|---|---|---|
Cy3Ga | Cy3G | Cy3GaECaf | Cy3GECaf | Gy3GZpC | Cy3GaEpC | Cy3GEpC | 合计 Total | |
白宝珠Baibaozhu | - | - | - | - | - | - | - | - |
粉宝珠Fenbaozhu | 7.13 | 51.68 | 1.45 | 0.93 | 3.42 | 5.68 | 29.70 | 100.00 |
红宝珠Hongbaozhu | 8.09 | 67.98 | 1.22 | 0.71 | 1.57 | 5.61 | 14.81 | 100.00 |
白芙蓉Baifurong | - | - | - | - | - | - | - | - |
粉芙蓉Fenfurong | 8.10 | 57.11 | 2.12 | 1.75 | 5.67 | 4.24 | 21.01 | 100.00 |
红芙蓉Hongfurong | 8.73 | 68.06 | 1.14 | 0.67 | 3.02 | 3.77 | 14.62 | 100.00 |
白嫦娥彩Baichangecai | - | - | - | - | - | - | - | - |
粉嫦娥彩Fenchangecai | 13.37 | 37.23 | 0.00 | 0.00 | 9.07 | 6.68 | 33.65 | 100.00 |
红嫦娥彩Hongchangecai | 15.45 | 50.66 | 0.00 | 0.00 | 5.17 | 6.55 | 22.17 | 100.00 |
白五宝Baiwubao | - | - | - | - | - | - | - | - |
粉五宝Fenwubao | 0.45 | 21.69 | 0.00 | 0.74 | 9.38 | 2.64 | 65.08 | 100.00 |
红五宝Hongwubao | 0.87 | 30.15 | 0.00 | 1.16 | 8.64 | 2.24 | 56.94 | 100.00 |
白碧玉Baibiyu | - | - | - | - | - | - | - | - |
粉碧玉Fenbiyu | 2.20 | 37.91 | 0.00 | 0.00 | 15.20 | 4.21 | 40.48 | 100.00 |
红碧玉Hongbiyu | 3.54 | 39.24 | 0.00 | 0.00 | 10.38 | 4.09 | 42.74 | 100.00 |
白七仙女Baiqixiannv | - | - | - | - | - | - | - | - |
粉七仙女Fenqixiannv | 2.73 | 38.05 | 0.00 | 0.00 | 8.70 | 4.27 | 46.25 | 100.00 |
红七仙女Hongqixiannv | 3.87 | 41.73 | 0.00 | 0.00 | 5.55 | 3.43 | 45.42 | 100.00 |
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