关键词: 白果, 蛋白, 过敏, 小鼠, 模型

Abstract:

【Objective】 Mouse model of allergic reaction induced by ginkgo kernel protein was studied for the allergen assessment. 【Method】 Taking Tris-HCl buffer solution as negative control and ovalbumin as positive control, different group of mice were sensitized by two doses of ginkgo kernel protein and controls orally on days 0, 7 and 14 d, and challenged intraperitioneally at 7 d after the last sensitization. 【Result】 The result showed there were high level of IgE and IgG in ginkgo kernel protein group and positive control group, histamine release rate after challenged in vitro was higher significantly than the negative control. During the immune time, histamine in plasma was increased. There were inflammatory focuses in intestines, lungs and livers in mice treated with thinner dose of ginkgo kernel protein, and also in kidneys of mice treated with thicker dose of gingko kernel protein and the positive control. 【Conclusion】 This model may be used to research IgE (Immunoglobin E)-mediatedⅠtype allergy in mice caused by ginkgo kernel protein.

Key words: gingko kernel, protein, allergy, mouse, model