关键词: 拟南芥, 大豆疫霉菌, 水杨酸, 系统获得抗性, 蛋白质组学

Abstract: System acquired resistance（SAR）is an important component of the complex disease resistance mechanism in plants. We carried out a proteomic analysis of the SAR of Arabidopsis induced by wound, salicylic acid (SA) and Phytophthora sojae. Total proteins prepared from Arabidopsis leaves were isolated by 2D PAGE. A total of 28 spots differentially expressed between the CK and treatment were identified by MALDI-TOF mass spectrometric analysis. Among them, 13 spots were up regulated and 11 spots were down regulated in the treated leaves; interestingly, the expression patterns of 4 spots in leaves induced by SA were opposite to that of wound and P.sojae. Functions of these proteins can be classified as metabolic enzymes, chaperone, oxidoreductase, defense-related proteins, protein kinase, chloroplast proteins and unknown function proteins. Studies of expression patterns of these proteins provide new information on the overlap of defense element and cross-communicating signal transduction pathways in SAR between wounding, SA and P.sojae attack. Proteome analysis is a useful approach to study the complex resistance mechanism in plants.

Key words: Arabidopsis thalinana, Phytophthora sojae, Salicylic acid（SA）, Systemic acquired resistance（SAR）, Proteomics