中国农业科学 ›› 2007, Vol. 40 ›› Issue (6): 1271-1275 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

缺氧对肉鸡心肌细胞内游离Ca2+浓度的影响

董世山,王迎春,马利芹,利 凯,张建军,欧德渊,赵立红,刘聚祥,乔 健   

  1. 河北农业大学动物科技学院
  • 收稿日期:2005-03-22 修回日期:1900-01-01 出版日期:2007-06-10 发布日期:2007-06-10
  • 通讯作者: 乔 健

Effect of Hypoxia on the Ca2+ Concentration in Broiler’s Cardiac Muscle Cell

  

  1. 河北农业大学动物科技学院
  • Received:2005-03-22 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10

摘要: 【目的】检测缺氧对肉鸡心肌细胞内Ca2+浓度的影响,阐明缺氧对心肌细胞影响的机制。【方法】利用体外细胞培养技术,以Fluo-3/AM为Ca2+指示剂,用LSCM检测缺氧对体外培养的肉鸡心肌细胞内游离Ca2+浓度的影响。【结果】与常氧组比较缺氧显著引起心肌细胞内游离Ca2+升高(常氧99.3±13.1;缺氧129.4±24.3,P<0.01),钙通道拮抗剂Verapamil(Ver)和Nifedipine(Nif)可显著抑制缺氧引起的游离Ca2+升高(缺氧+Ver 100.9±28.2,缺氧+Nif 107.6±27.7)。【结论】缺氧能够增强心肌细胞的游离Ca2+跨膜转运导致细胞内Ca2+浓度升高,Ca2+通道拮抗剂能够减弱缺氧引起的心肌细胞膜上Ca2+的跨膜转运。

关键词: 肉鸡, 心肌细胞, 游离Ca2+, 缺氧

Abstract: The purpose of this experiment was to study the effect of hypoxia on the Ca2+ concentration in broiler’s Cardiac Muscle Cell (CMC) . The concentration of Ca2+ in the CMC was observed by laser scanning confocal microscope (LSCM). The results showed that hypoxia could significantly increase intracellular Ca2+ of CMC (CMC: normal oxgen:99.3±13.1, hypoxia:129.4±24.3,p<0.01.). The Ca2+ antagonist (nifedipine, Nif; verapamil, Ver) can significantly restrain the Ca2+ influx across the cell membrane of CMC treated by hypoxia (CMC: hypoxia + Ver:100.9±28.2, hypoxia+Nif:107.6±27.7, p<0.01). Hypoxia is the important cause of ascites syndrome(AS), myocardial hypertrophy is the typical pathological change .The results suggest that the transmembrane movement of Ca2+ in the CMC maybe play an important role in the development of AS .

Key words: Broiler Chicke, Cardiac Muscle Cell, Ca2+, Hypoxia