关键词: 芝麻, 空间诱变, AFLP, DNA指纹

Abstract: Abstract：【OBJECTIVE】To reveal differences of sesame space mutantlines in DNA level and discuss their molecular mechanisms which provide theory basis for crop space-flight mutation breeding.【METHOD】DNA fingerprint analysis was performed between three mutantlines and their original CK line “Yuzgi No.4” by amplified fragments length polymorphism (AFLP) technology for the first time.【RESULTS】Three mutantlines and their CK line were analysed by random selecting 30 pairs AFLP primers, 625 polymorphic bands were produced in 1176 DNA bands which were obtained in four samples, and its degree of gene polymorphism was 53.15%. DNA polymorphic bands were amplified between three mutantlines and their CK line during every primer pairs. UPGMA clustering showed that three mutantlines which were similar had biggish differences by comparing with “Yuzhi No.4”. 【CONCLUSSION】Space-flight mutagenesis could result in variation of sesame germ plasm, so space-flight mutantion breeding was an effective approach of plant breeding. AFLP was also a high efficient molecular biological method of researching plant space-flight mutagenesis.

Key words: Sesame (Sesamum indicum), Space mutation, AFLP, DNA fingerprint