关键词: 水稻, 种子休眠性, 重组自交系, 数量性状基因座, 干热破除休眠

Abstract: Quantitative trait loci (QTL) controlling seed dormancy in rice were identified using recombinant inbred lines (RILs) population derived from the cross between a japonica variety Kinmaze and an indica variety DV85. Seeds of two parents and each RIL were harvested in 35d after heading. The germination percentage of these seeds at 30℃ for 7 days were measured as the degree of seed dormancy. QTL analysis was performed with Windows QTL Cartographer 1.13a program by composite interval mapping. A total of four QTL for seed dormancy were detected on chromosome 2 (two regions), 5 and 11, respectively. Phenotypic variation explained by each QTL ranged from 8.37% to 17.40%. Responses of such loci to a dormancy-broken with a dry heat treatment were further detected. The results showed that two alleles of qDOR-2-1 and qDOR-5 from DV85 as well as the allele of qDOR-11 from Kinmaze increased the seed dormancy, which seemed to be easily broken by dry heat treatment. Such loci of seed dormancy may be applied to rice genetic improvement. The allele of qDOR-2-2 from DV85 increased the seed dormancy, which could not be broken by dry heat treatment.

Key words: Rice, Seed dormancy, RI population, QTL, Dormancy-broken by dry heat treatment