中国农业科学 ›› 2004, Vol. 37 ›› Issue (10): 1544-1549 .

• 园艺 • 上一篇    下一篇

超高压杀菌条件的响应曲面法优化研究

高瑀珑,江汉湖,王允祥   

  1. 南京农业大学食品科技学院
  • 收稿日期:2003-11-24 修回日期:2004-03-18 出版日期:2004-10-20 发布日期:2004-10-20
  • 通讯作者: 江汉湖

Optimization of Inactivation Conditions of High Hydrostatic Pressure Using Response Surface Methodology

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  1. 南京农业大学食品科技学院
  • Received:2003-11-24 Revised:2004-03-18 Online:2004-10-20 Published:2004-10-20

摘要: 采用响应曲面法(response surface methodology,RSM)建立了超高压杀灭大肠杆菌的二次多项数学模型,验证了模型的有效性,并考察温度、压力和保压时间对超高压杀灭大肠杆菌的影响,优化出杀灭6个数量级大肠杆菌ATCC8739的工艺参数温度为32.2℃,压力346.4 MPa,保压时间12.6 min。

关键词: 超高压杀菌, 大肠杆菌, 响应曲面法, 优化, 工艺参数

Abstract: Response surface methodology (RSM) was employed in the present work and a second order quadratic equation for high hydrostatic pressure (HHP) inactivation was built. The adequacy of the model equation for predicting the optimum response values was verified effectively by the validation data. Effects of temperature, pressure, and pressure holding time on HHP inactivation of Escherichia coli were explored. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the optimum process parameters for inactivation of Escherichia coli ATCC 8739 of six log cycles were obtained as: temperature 32.2℃, pressure 346.4 MPa, and pressure holding time 12.6 min.

Key words: High hydrostatic pressure(HHP) inactivation, Escherichia coli, Response surface methodology (RSM), Optimization, Process parameter