彩色稻叶片光合特征及其与抗氧化酶活性、花青素含量间关系

doi:10.3864/j.issn.0578-1752.210289

摘要/Abstract

摘要： 【目的】近年来，彩色稻因其独特的外观和特殊的营养价值在观光农业和专用营养大米产业中快速发展。然而，彩色稻叶片光合作用对光的响应机理还有待阐明。【方法】以4个叶色不同的水稻品种，包括深紫叶（deep purple, DP），淡绿叶（fale green, FG），深绿叶/常规叶色（dark green, DG）及淡紫叶（fale purple, FP）为材料，研究不同叶色叶片光合作用的变化特征，并揭示其作用机制。【结果】深紫叶水稻叶片叶绿素、类胡萝卜素含量最高，其次分别是深绿叶及淡紫叶水稻品种，而淡绿叶水稻叶片叶绿素、类胡萝卜素含量最低。淡绿叶水稻叶片实际光量子效率（PSII）、净光合速率、核酮糖-1,5-二磷酸羧化酶活性和干物质积累量最高，显著高于其他品种，但其过氧化氢和丙二醛含量显著低于其他叶色水稻品种。深紫叶与深绿叶水稻叶片叶绿素、类胡萝卜素含量没有显著性差异，但深紫叶水稻叶片PSI和PSII实际荧光量子效率、净光合速率、核酮糖-1,5-二磷酸羧化酶活性均显著高于深绿叶水稻。相应地，深紫色水稻叶片抗氧化酶活性显著高于深绿叶水稻，但其过氧化氢和丙二醛含量显著低于深绿叶水稻，推测与深紫叶水稻叶片花青素含量显著高于深绿叶水稻有关。外源喷施花青素试验进一步证实了这个假设，即外源花青素可一定程度上降低过氧化氢含量，提高叶片光合速率。【结论】鉴此，花青素可减轻自然条件下光量子过剩对水稻叶片光合作用的抑制，研究结果可为水稻高光效育种及彩色稻产量品质协同栽培技术研发提供理论基础。

关键词: 彩色水稻, 叶绿素, 光合作用, 抗氧化力, 花青素

Abstract: 【Objective】In recent years, due to its unique appearance and special nutritional value, colored rice has developed rapidly in the sightseeing agriculture, selection and production of special nutritional rice. However, the mechanism underlying the photosynthetic response of leaves in colored rice to light remains to be elucidated.【Method】In this experiment, four rice varieties with different chlorophyll content in leaves were selected in this experiment, that is deep purple (DP), fale green (FG), dark green (DG) and fale purple (FP). The change characteristics of leaf photosynthesis were studied and response mechanisms were revealed.【Result】The results indicated that the highest chlorophyll content was showed in DP, followed by the DG and FP, while the lowest value were found in FG. Under this case, the FG variety attained the highest value of actual fluorescence quantum efficiency of PSII, photosynthetic rate, ribulose-1, 5-bisphosphate carboxylase activity and total dry matter weight, which was significantly higher than the other varieties. The content of hydrogen peroxide and malondialdehyde in FG leaves was significantly lower than other rice varieties. Besides, there is no significant difference in leaf chlorophyll content and carotenoid content between DP and DG, while the actual fluorescence quantum efficiency of PSII, net photosynthetic rate, ribulose-1,5-diphosphate carboxylase activity in leaves of DP were significantly higher than those of DG. Correspondingly, activities of antioxidant enzymes in leaves of DP were significantly higher than those of DG, while the content of hydrogen peroxide and malondialdehyde were significantly lower than DG. This may be related to the anthocyanin content in the leaves, because the anthocyanin content in the leaves of DP was significantly higher than that of DG. The leaf photosynthetic rate was significantly increased and hydrogen peroxide content was significantly decreased after foliar application of anthocyanin. 【Conclusion】Therefore, it was inferred that anthocyanin might exert important role in alleviating the inhibition effect caused by excess opical quantum on the photosynthesis of leaves under natural conditions. The results could provide a theoretical basis for rice high light efficiency breeding and exploitation for high quality and high efficiency cultivation technology of colored rice.

Key words: colored rice, chlorophyll, photosynthesis, antioxidant capacity, anthocyanin

陈婷婷, 符卫蒙, 余景, 奉保华, 李光彦, 符冠富, 陶龙兴. 彩色稻叶片光合特征及其与抗氧化酶活性、花青素含量间关系[J]. 中国农业科学, 0, (): 1-.

CHEN TingTing, FU WeiMeng, YV Jing, FENG BaoHua, LI GuangYan, FU GuanFu, TAO LongXing. The Photosynthesis Characteristics of Rice Leaves with Different Colors and its Relation with Antioxidant Capacity, Anthocyanin Content[J]. Scientia Agricultura Sinica, 0, (): 1-.

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彩色稻叶片光合特征及其与抗氧化酶活性、花青素含量间关系

The Photosynthesis Characteristics of Rice Leaves with Different Colors and its Relation with Antioxidant Capacity, Anthocyanin Content

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