水稻抗褐飞虱基因QBPH1和QBPH4的精细定位与聚合效应分析

doi:10.3864/j.issn.0578-1752.2024.23.004

中国农业科学 ›› 2024, Vol. 57 ›› Issue (23): 4619-4631.doi: 10.3864/j.issn.0578-1752.2024.23.004

• 专题：作物抗病虫相关基因的挖掘与利用 • 上一篇下一篇

水稻抗褐飞虱基因QBPH1和QBPH4的精细定位与聚合效应分析

熊尚烨¹(), 张翔¹, 梁宝慧¹, 叶仰东¹, 李浴阳¹, 朱晓¹, 朱志鸿¹, 官华忠³, 张帅¹^,²(), 吴建国¹^,²(), 胡杰¹^,²()

¹ 福建农林大学植物保护学院/闽台作物有害生物生态防控国家重点实验室/媒介病毒研究中心，福州 350002
² 福建农林大学未来技术学院/遗传改良研究中心，福州 350002
³ 福建农林大学农学院/福建省作物设计育种重点实验室，福州 350002

收稿日期:2024-05-10 接受日期:2024-06-17 出版日期:2024-12-01 发布日期:2024-12-07
通信作者:
张帅，E-mail：zhangshuai@fafu.edu.cn。
吴建国，E-mail：wujianguo81@126.com。
胡杰，E-mail：jackhujie1526@163.com
联系方式: 熊尚烨，E-mail：203076599@qq.com。
基金资助:
国家自然科学基金(32025031); 国家自然科学基金(U1905203); 福建省自然科学基金(2021J01071); 福建农林大学校级创新基金(KFA20006A); 福建农林大学闽台作物有害生物生态防控国家重点实验室开放基金(SKL2023001); 崖州湾种子实验室与中国种业股份有限公司联合开放竞争项目(B23YQ1514); 崖州湾种子实验室与中国种业股份有限公司联合开放竞争项目(B23CQ15EP)

Fine Mapping and Analysis of Pyramiding Effects of Rice Brown Planthopper Resistance Genes QBPH1 and QBPH4

XIONG ShangYe¹(), ZHANG Xiang¹, LIANG BaoHui¹, YE YangDong¹, LI YuYang¹, ZHU Xiao¹, ZHU ZhiHong¹, GUAN HuaZhong³, ZHANG Shuai¹^,²(), WU JianGuo¹^,²(), HU Jie¹^,²()

¹ College of Plant Protection, Fujian Agriculture and Forestry University/State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/Vector-Borne Virus Research Center, Fuzhou 350002
² School of Future Technology, Fujian Agriculture and Forestry University/Center for Genetic Improvement, Fuzhou 350002
³ College of Agriculture, Fujian Agriculture and Forestry University/Fujian Key Laboratory of Crop Breeding by Design and Key Laboratory of Genetics, Fuzhou 350002

Received:2024-05-10 Accepted:2024-06-17 Published:2024-12-01 Online:2024-12-07

摘要/Abstract

摘要：

【目的】利用遗传群体发掘新的水稻抗褐飞虱QTL，并评估其聚合效应，为抗虫育种提供重要的遗传材料和基因资源。【方法】利用日本晴（感虫型）和佳辐占（抗虫型）的重组自交系（RILs）群体，结合苗期抗性评价和极端混池测序法（BSA-seq）定位抗褐飞虱QTL。进一步通过QTL精细定位、候选基因鉴定、聚合效应分析及混池转录组测序（BSR-seq），揭示QTL介导的抗性生理和分子机制。aaaa【结果】 BSA-seq分析，在水稻第1（30—32 Mb区段）和第4（5—7 Mb区段）染色体上定位到2个主效抗性QTL，分别命名为QBPH1和QBPH4。通过区间连锁的分子标记，验证了这些QTL的真实性。QBPH4与已克隆的BPH3和BPH15位于同一位置，而QBPH1为一个新发现的QTL。通过加密标记和重组单株分析，将QBPH1的位置精确到30.61—30.65 Mb。在此区域内，Os01g53294和Os01g53330分别编码呼吸爆发氧化酶蛋白B和花青素5,3-O-葡萄糖基转移酶，被认为是QBPH1的可靠候选基因。此外，通过对比QBPH1和QBPH4单基因及其聚合家系在苗期的抗性、褐飞虱蜜露面积和重量、虫增重、致死率、偏好性等指标，发现双基因聚合家系的抗性未显著增强。QBPH1和QBPH4均介导了抗生性和趋避性的生理机制，但其效应存在差异。BSR-seq分析揭示了QBPH1的不同等位基因型在转录调控、蛋白质氨基酸磷酸化和氧化还原过程中的差异表达基因（DEGs）显著富集。此外，与茉莉酸（jasmonic acid，JA）合成和信号传导路径相关的基因在抗虫材料中得到显著上调，并通过RT-qPCR试验得到了验证。【结论】成功在水稻第1染色体上鉴定了一个新的抗褐飞虱QTL——QBPH1。该QTL介导了水稻对褐飞虱的抗生性和趋避性，但与另一QTL的聚合效应并不显著。QBPH1可能通过参与JA通路来介导对褐飞虱的防御机制。在此基础上，Os01g53294和Os01g53330被确定为QBPH1的可靠候选基因。

关键词: 水稻, 褐飞虱抗性, QTL定位, 基因聚合, 转录组测序

Abstract:

【Objective】 This study aims to discover new quantitative trait loci (QTLs) for resistance to the brown planthopper (BPH) in rice by utilizing a genetic population and to assess the pyramiding effects of these QTLs, thereby providing crucial genetic materials and resources for breeding insect-resistant rice. 【Method】 A recombinant inbred line (RIL) population derived from a cross between susceptible NPB and resistant Jiafuzhan was used, combined with seedling stage resistance evaluation and extreme mixed pool sequencing (BSA-seq) to map BPH resistance QTLs. Further, fine mapping, candidate gene identification, pyramiding effect analysis, and mixed-pool transcriptome sequencing (BSR-seq) were employed to elucidate the physiological and molecular mechanisms mediated by these QTLs. 【Result】 BSA-seq identified two major resistance QTLs on chromosomes 1 (30-32 Mb) and 4 (5-7 Mb), named QBPH1 and QBPH4, respectively. Interval-linked molecular markers confirmed the authenticity of these QTLs. QBPH4 coincides with previously cloned BPH3 and BPH15, while QBPH1 represents a newly discovered QTL. High-density markers and recombinant analysis further narrowed the QBPH1 region to 30.61-30.65 Mb. This analysis identified Os01g53294 and Os01g53330 as reliable candidate genes, which code for a respiratory burst oxidase protein B and an anthocyanin 5,3-O-glucosyltransferase, respectively. In evaluations of seedling resistance, brown planthopper honeydew area and weight, insect weight gain, lethality, and preference, comparisons between QBPH1 and QBPH4 single genes and their polymerized lines revealed no significant enhancement in resistance. Both QBPH1 and QBPH4 mediated antibiosis and antixenosis mechanisms with varying effects. BSR-seq analysis highlighted significant enrichment of differentially expressed genes (DEGs) involved in transcriptional regulation, protein phosphorylation, and redox processes among different QBPH1 alleles. Additionally, genes associated with jasmonic acid (JA) synthesis and signaling pathways were significantly upregulated in resistant materials, confirmed by RT-qPCR experiments. 【Conclusion】 A novel BPH resistance QTL, QBPH1, was successfully identified on chromosome 1 of rice. QBPH1 mediated both antibiosis and avoidance against BPH, though its aggregation effect with another QTL was not significant. QBPH1 may mediate defense mechanisms against BPH through involvement in the JA pathway. Based on this, Os01g53294 and Os01g53330 have been validated as reliable candidate genes for QBPH1.

Key words: rice, brown planthopper resistance, QTL mapping, gene pyramiding, RNA-seq

熊尚烨, 张翔, 梁宝慧, 叶仰东, 李浴阳, 朱晓, 朱志鸿, 官华忠, 张帅, 吴建国, 胡杰. 水稻抗褐飞虱基因QBPH1和QBPH4的精细定位与聚合效应分析[J]. 中国农业科学, 2024, 57(23): 4619-4631.

XIONG ShangYe, ZHANG Xiang, LIANG BaoHui, YE YangDong, LI YuYang, ZHU Xiao, ZHU ZhiHong, GUAN HuaZhong, ZHANG Shuai, WU JianGuo, HU Jie. Fine Mapping and Analysis of Pyramiding Effects of Rice Brown Planthopper Resistance Genes QBPH1 and QBPH4 [J]. Scientia Agricultura Sinica, 2024, 57(23): 4619-4631.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2024.23.004

https://www.chinaagrisci.com/CN/Y2024/V57/I23/4619

图/表 8

表1

图1

图2

图3

图4

图5

图6

表2

参考文献 30

[1]	ATHWAL D S, PATHAK M D, BACALANGCO E H, PURA C D. Genetics of resistance to brown planthoppers and green leafhoppers in Oryza sativa L.. Crop Science, 1971, 11(5): 747-750.
[2]	YANG K, LIU H M, JIANG W H, HU Y X, ZHOU Z Y, AN X, MIAO S, QIN Y S, DU B, ZHU L L, HE G C, CHEN R Z. Large scale rice germplasm screening for identification of novel brown planthopper resistance sources. Molecular Breeding, 2023, 43(9): 70.
[3]	CHENG X Y, ZHU L L, HE G C. Towards understanding of molecular interactions between rice and the brown planthopper. Molecular Plant, 2013, 6(3): 621-634. doi: 10.1093/mp/sst030 pmid: 23396040
[4]	ZHAO Y, HUANG J, WANG Z Z, JING S L, WANG Y, OUYANG Y D, CAI B D, XIN X F, LIU X, ZHANG C X, PAN Y F, MA R, LI Q F, JIANG W H, ZENG Y, SHANGGUAN X X, WANG H Y, DU B, ZHU L L, XU X, FENG Y Q, HE S Y, CHEN R Z, ZHANG Q F, HE G C. Allelic diversity in an NLR gene BPH9 enables rice to combat planthopper variation. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(45): 12850-12855.
[5]	LIU Y Q, WU H, CHEN H, LIU Y L, HE J, KANG H Y, SUN Z G, PAN G, WANG Q, HU J L, ZHOU F, ZHOU K N, ZHENG X M, REN Y L, CHEN L M, WANG Y H, ZHAO Z G, LIN Q B, WU F Q, ZHANG X, GUO X P, CHENG X N, JIANG L, WU C Y, WANG H Y, WAN J M. A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice. Nature Biotechnology, 2015, 33(3): 301-305. doi: 10.1038/nbt.3069 pmid: 25485617
[6]	GUO J P, XU C X, WU D, ZHAO Y, QIU Y F, WANG X X, OUYANG Y D, CAI B D, LIU X, JING S L, SHANGGUAN X X, WANG H Y, MA Y H, HU L, WU Y, SHI S J, WANG W L, ZHU L L, XU X, CHEN R Z, FENG Y Q, DU B, HE G C. Bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice. Nature Genetics, 2018, 50(2): 297-306.
[7]	DU B, ZHANG W L, LIU B F, HU J, WEI Z, SHI Z Y, HE R F, ZHU L L, CHEN R Z, HAN B, HE G C. Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(52): 22163-22168.
[8]	CHENG X Y, WU Y, GUO J P, DU B, CHEN R Z, ZHU L L, HE G C. A rice lectin receptor-like kinase that is involved in innate immune responses also contributes to seed germination. The Plant Journal, 2013, 76(4): 687-698. doi: 10.1111/tpj.12328 pmid: 24033867
[9]	JI H, KIM S R, KIM Y H, SUH J P, PARK H M, SREENIVASULU N, MISRA G, KIM S M, HECHANOVA S L, KIM H, LEE G S, YOON U H, KIM T H, LIM H, SUH S C, YANG J, AN G, JENA K K. Map-based cloning and characterization of the BPH18 gene from wild rice conferring resistance to brown planthopper (BPH) insect pest. Scientific Reports, 2016, 6: 34376.
[10]	TAMURA Y, HATTORI M, YOSHIOKA H, YOSHIOKA M, TAKAHASHI A, WU J Z, SENTOKU N, YASUI H. Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52. Scientific Reports, 2014, 4: 5872.
[11]	WANG Y, CAO L M, ZHANG Y X, CAO C X, LIU F, HUANG F K, QIU Y F, LI R B, LOU X J. Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice. Journal of Experimental Botany, 2015, 66(19): 6035-6045.
[12]	SHI S J, WANG H Y, NIE L Y, TAN D, ZHOU C, ZHANG Q, LI Y, DU B, GUO J P, HUANG J, WU D, ZHENG X H, GUAN W, SHAN J H, ZHU L L, CHEN R Z, XUE L J, WALLING L L, HE G C. Bph30 confers resistance to brown planthopper by fortifying sclerenchyma in rice leaf sheaths. Molecular Plant, 2021, 14(10): 1714-1732.
[13]	REN J S, GAO F Y, WU X T, LU X J, ZENG L H, LV J Q, SU X W, LUO H, REN G J. Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice. Scientific Reports, 2016, 6: 37645. doi: 10.1038/srep37645 pmid: 27876888
[14]	ZHOU C, ZHANG Q, CHEN Y, HUANG J, GUO Q, LI Y, WANG W S, QIU Y F, GUAN W, ZHANG J, GUO J P, SHI S J, WU D, ZHENG X H, NIE L Y, TAN J Y, HUANG C M, MA Y H, YANG F, FU X Q, DU B, ZHU L L, CHEN R Z, LI Z K, YUAN L P, HE G C. Balancing selection and wild gene pool contribute to resistance in global rice germplasm against planthopper. Journal of Integrative Plant Biology, 2021, 63(10): 1695-1711. doi: 10.1111/jipb.13157
[15]	GUO J P, WANG H Y, GUAN W, GUO Q, WANG J, YANG J, PENG Y X, SHAN J H, GAO M Y, SHI S J, SHANGGUAN X X, LIU B F, JING S L, ZHANG J, XU C X, HUANG J, RAO W W, ZHENG X H, WU D, ZHOU C, DU B, CHEN R Z, ZHU L L, ZHU Y X, WALLING L L, ZHANG Q F, HE G C. A tripartite rheostat controls self-regulated host plant resistance to insects. Nature, 2023, 618(7966): 799-807.
[16]	WU D, GUO J P, ZHANG Q, SHI S J, GUAN W, ZHOU C, CHEN R Z, DU B, ZHU L L, HE G C. Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls. New Phytologist, 2022, 234(3): 1031-1046. doi: 10.1111/nph.18012 pmid: 35119102
[17]	KOBAYASHI T. Evolving ideas about genetics underlying insect virulence to plant resistance in rice-brown planthopper interactions. Journal of Insect Physiology, 2016, 84: 32-39. doi: S0022-1910(15)30010-X pmid: 26668110
[18]	HU W, XIAO H X, HU K, JIANG Y J, ZHANG Y. Application of marker-assisted backcross to introgress Bph3, Bph14 and Bph15 into an elite indica rice variety for improving its resistance to brown planthopper. Plant Breeding, 2016, 135(3): 291-300.
[19]	HU J, XIAO C, CHENG M X, GAO G J, ZHANG Q L, HE Y Q. Fine mapping and pyramiding of brown planthopper resistance genes QBph3 and QBph4 in an introgression line from wild rice O. officinalis. Molecular Breeding, 2015, 35(1): 3.
[20]	SHARMA P N, TORII A, TAKUMI S, MORI N, NAKAMURA C. Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stål) resistance genes Bph1 and Bph2 on rice chromosome 12. Hereditas, 2004, 140(1): 61-69.
[21]	HU J, CHANG X Y, ZOU L, TANG W Q, WU W R. Identification and fine mapping of Bph33, a new brown planthopper resistance gene in rice (Oryza sativa L.). Rice, 2018, 11(1): 55.
[22]	HUANG Z, HE G, SHU L, LI X, ZHANG Q. Identification and mapping of two brown planthopper resistance genes in rice. Theoretical and Applied Genetics, 2001, 102(6): 929-934.
[23]	NAIK S B, DIVYA D, SAHU N, SUNDARAM R M, SARAO P S, SINGH K, LAKSHMI V J, BENTUR J S. A new gene Bph33(t) conferring resistance to brown planthopper (BPH), Nilaparvata lugens (Stål) in rice line RP2068-18-3-5. Euphytica, 2018, 214(3): 53.
[24]	YANG M, CHENG L, YAN L H, SHU W, WANG X Y, QIU Y F. Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64. Hereditas, 2019, 156: 22. doi: 10.1186/s41065-019-0098-4 pmid: 31297040
[25]	BALACHIRANJEEVI C H, PRAHALADA G D, MAHENDER A, JAMALODDIN M, SEVILLA M A L, MARFORI-NAZAREA C M, VINARAO R, SUSHANTO U, BAEHAKI S E, LI Z K, ALI J. Identification of a novel locus, BPH38(t), conferring resistance to brown planthopper (Nilaparvata lugens Stal.) using early backcross population in rice (Oryza sativa L.). Euphytica, 2019, 215(11): 185.
[26]	QI J F, LI J C, HAN X, LI R, WU J Q, YU H X, HU L F, XIAO Y T, LU J, LOU Y G. Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice. Journal of Integrative Plant Biology, 2016, 58(6): 564-576. doi: 10.1111/jipb.12436
[27]	ZENG J M, ZHANG T F, HUANGFU J Y, LI R, LOU Y G. Both allene oxide synthases genes are involved in the biosynthesis of herbivore-induced jasmonic acid and herbivore resistance in rice. Plants, 2021, 10(3): 442.
[28]	WANG R, SHEN W B, LIU L L, JIANG L, LIU Y Q, SU N, WAN J M. A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack. Plant Molecular Biology, 2008, 66(4): 401-414. doi: 10.1007/s11103-007-9278-0 pmid: 18185911
[29]	MA F L, YANG X F, SHI Z Y, MIAO X X. Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing-sucking insect in rice. New Phytologist, 2020, 225(1): 474-487. doi: 10.1111/nph.16111 pmid: 31407341
[30]	XU J, WANG X J, ZU H Y, ZENG X, BALDWIN I T, LOU Y G, LI R. Molecular dissection of rice phytohormone signaling involved in resistance to a piercing-sucking herbivore. New Phytologist, 2021, 230(4):1639-1652.

引物 primer	正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)	用途 Purpose
C1-30.40	GAGACTAGTGCTTCCTAGTAG	CCAGATTTCTTTATAAGAAGCATG	定位 Mapping
C1-30.50	TCGGCCAGCTGATACGGTATCG	TTAGAACAAGCCAAAATAGTCGTT
C1-30.61	TGCAACATAGGTGCTTTGCC	CCTGCCAGCAGCCTAACTTA
C1-30.65	CCCTACACACATTGGCATGC	AGGCTCTTTTGTTCACTTTACACA
RM128	TTCTTGGAAGCGAAGAGTGAGG	CCTCCTTGTGCTCAGCCATGC
C1-30.77	AAACCCCTGAAGTGAATCGC	CATCGCCGAAGTCGTTCAC
C1-30.86	TGTTAGGGTGGCTGTTGACT	GGAACAACCACAGGAACACA
C1-30.90	TCTCGTGGTTTCCAGACGAA	ACTGGGATGGTTCAGACCTC
C1-31.00	TTCATTGCGCCTTTCAGTCCCAT	GAGTGGACTGTAGAGATCTCCCAC
C1-31.64	ATGCAGCCGGTCTCGTC	TCGCCAAGGTGCTCAGCTC
C1-31.72	AGGAGAACTTCAGAGTGGCG	CGTCCGCGGTGAAG
C1-31.76	ACCGGGGCCTAACTAGTCTA	GCGGAAAAACGGGGAGAAAG
OsPR1a	GTCTTCATCACCTGCAACTACTC	CATGCATAAACACGTAGCATAGCA	RT-qPCR
JIOsPR10	GCAGCGTCAGGCAGTTCAA	GAACTCCAGCCTCTCCTTCATG
OsJAZ8	GTCGCCCACCATCAACAGCA	CCGTTGGTGTCATCACTCTT
OsNAC10	TCCTTTGATGATGGCAGCAGCA	TGCGGTTTCTTGACAACGGCAT
ONAC131	AATATATCCAAACCAAACACTA	AGCAGCGGTTTCTTGGCAACAT
OsJAMyb	GAAGAGGACTGGGAAGAGCTG	GCTATCTTGGACCATCGGTTGC
OsLOX1	GCATCCCCAACAGCACATC	AAGATTTGGGAGTGACATATTGG

基因名称 Locus name	MR	MS	BR	BS	基因注释 Gene annotation
LOC_Os01g53294	6.58	7.92	3.09	2.08	呼吸爆发氧化酶蛋白B Respiratory burst oxidase protein B
LOC_Os01g53330	1.28	1.32	3.75	1.83	花青素5,3-O-葡萄糖基转移酶Anthocyanidin 5,3-O-glucosyltransferase

水稻抗褐飞虱基因QBPH1和QBPH4的精细定位与聚合效应分析

Fine Mapping and Analysis of Pyramiding Effects of Rice Brown Planthopper Resistance Genes QBPH1 and QBPH4

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 30

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	许娜, 唐颖, 徐正进, 孙健, 徐铨. 籼粳杂种不育的遗传分析和候选基因鉴定[J]. 中国农业科学, 2024, 57(8): 1417-1429.
[2]	陈兵先, 张琪, 戴彰言, 周旭, 刘军. 水杨酸引发提高低温下水稻种子萌发活力的生理与分子效应[J]. 中国农业科学, 2024, 57(7): 1220-1236.
[3]	宋雅荣, 常单娜, 周国朋, 高嵩涓, 段廷玉, 曹卫东. 解磷细菌活化水稻土中低品位磷矿粉的效果与机制[J]. 中国农业科学, 2024, 57(6): 1102-1116.
[4]	张必东, 林泓, 朱思颖, 李忠成, 庄慧, 李云峰. 水稻颖壳异常突变体ah1的鉴定与候选基因分析[J]. 中国农业科学, 2024, 57(3): 429-441.
[5]	刁志娟, 陈立喆, 王勋, 鲁玲, 刘燕, 张静, 夏娜, 唐定中, 李生平. 胱硫醚-β-合成酶OsCBSX4调控水稻稻瘟病抗性的机理[J]. 中国农业科学, 2024, 57(23): 4593-4606.
[6]	赵婕, 赵龙缘, 潘凝辉, 管丽蓉, 杜云龙, 李成云, 王云月, 谢勇. 水解酶基因BGIOSGA023826在稻瘟菌侵染过程中的抗病表型效应[J]. 中国农业科学, 2024, 57(23): 4607-4618.
[7]	吴传磊, 胡晓渝, 王伟, 苗龙, 白鹏宇, 王郭伋, 李娜, 舒阔, 邱丽娟, 王晓波. 大豆油分相关功能基因分子标记开发与鉴定及优异等位变异聚合分析[J]. 中国农业科学, 2024, 57(22): 4402-4415.
[8]	刘苡含, 牟青山, 贺响, 陈敏, 胡晋, 关亚静. OsFWL3调控水稻金属离子转运和积累的研究[J]. 中国农业科学, 2024, 57(21): 4161-4174.
[9]	蔡玉彪, 张坤杰, 王雅宣, 赖凤香, 何佳春, 万品俊, 傅强. 水稻品种对褐飞虱趋向二化螟危害株习性的影响[J]. 中国农业科学, 2024, 57(20): 3998-4006.
[10]	马荆鄂, 熊信威, 周敏, 吴斯琪, 韩甜, 饶友生, 王樟凤, 许继国. 基于垂体全长转录组测序分析鸡睾丸性状相关基因[J]. 中国农业科学, 2024, 57(20): 4130-4144.
[11]	张亚玲, 付忠举, 李雪, 孙宇佳, 赵羽涵, 顾欣怡, 王艳霞, 靳学慧, 吴伟怀, 华丽霞. 黑龙江、四川和海南省稻作区水稻穗腐病病原比较分析[J]. 中国农业科学, 2024, 57(2): 278-294.
[12]	郭乃辉, 张文忠, 圣忠华, 胡培松. 利用CRISPR/Cas9技术编辑MODD增强水稻休眠性[J]. 中国农业科学, 2024, 57(2): 227-235.
[13]	翁文安, 邢志鹏, 胡群, 魏海燕, 史扬杰, 奚小波, 李秀丽, 刘桂云, 陈娟, 袁风萍, 孟轶, 廖萍, 高辉, 张洪程. 无人化旱直播模式对水稻产量、品质和经济效益的影响[J]. 中国农业科学, 2024, 57(17): 3350-3365.
[14]	苏素苗, 康天恺, 邹家龙, 汪本福, 张洋洋, 廖世鹏, 李小坤. 不同水稻品种和施锌方式对水稻产量及籽粒锌有效性的影响[J]. 中国农业科学, 2024, 57(15): 3023-3034.
[15]	鄢柳慧, 钟琦, 马增凤, 韦敏益, 刘驰, 秦媛媛, 周小龙, 黄大辉, 卢颖萍, 秦钢, 张月雄. 普通野生稻早抽穗基因OsEHD8的鉴定与进化分析[J]. 中国农业科学, 2024, 57(14): 2703-2716.