不同基因型花生耐荫性评价及其鉴定指标的筛选

doi:10.3864/j.issn.0578-1752.2020.06.006

摘要/Abstract

摘要：

【目的】分析不同基因型花生耐荫性,筛选耐荫鉴定指标,建立耐荫评价模型,为花生耐荫资源筛选和品种选育提供理论支撑。【方法】采用田间试验,以30个花生品种（系）和1个玉米品种为试验材料,设计玉米间作花生和净作花生,在花生结荚期测定花生叶片净光合速率（X₁₃）、气孔导度（X₁₄）、胞间CO₂浓度（X₁₅）和蒸腾速率（X₁₆）;成熟期测定主茎高（X₁）、侧枝长（X₂）、总分枝数（X₃）、有效分枝数（X₄）,单株结果数（X₅）、单株饱果数（X₆）和单株产量（X₁₁）;收获晾晒干后测定其百果重（X₇）、百果仁重（X₈）、百仁重（X₉）、出仁率（X₁₀）和小区产量（X₁₂）。根据间作遮荫和净作条件下的各单项指标的耐荫系数,采用主成分分析、聚类分析、逐步回归分析和隶属函数法等多元统计分析法,对花生耐荫性进行综合评价。【结果】不同花生品种（系）各单项指标的耐荫系数变异幅度不同,除出仁率外各个单项指标间存在显著或极显著的相关性。通过主成分分析将16个单项指标转换为5个相互独立的综合指标,其贡献率分别为33.860%、26.666%、11.176%、8.471%和6.954%,代表了全部数据87.127%的信息量。通过隶属函数分析,对于综合指标CI₁—CI₅,其隶属函数值最大的分别是6-2、天府29号、201150118A、201240413和闽花6号。对耐荫综合评价值（D）进行聚类分析,将30个花生品种（系）划分为3类,第一类属于耐荫型,包含11个品种（系）,第二类属于中度耐荫型,包含18个品种（系）,第三类属于敏感型,包含1个品种（系）。通过逐步回归分析建立花生耐荫性评价最优数学模型,D=-0.741+0.576X₉+0.507X₁₁+0.298X₁₃+0.272X₁₂+0.406X₁₀（R²=0.990）,估计精度在93.18%以上,筛选出5个鉴定花生耐荫性指标,分别为百仁重、出仁率、单株产量、小区产量和净光合速率。对参试材料耐荫类别特征分析可知,耐荫型花生净光合速率较高,百仁重、单株产量、小区产量和出仁率高,而敏感型百仁重、单株产量、净光合速率、小区产量和出仁率均最低。【结论】采用多元统计分析法对花生耐荫性进行评价分析是较为科学的,30个花生品种（系）被分成3类（耐荫型、中度耐荫型和敏感型）;百仁重、出仁率、单株产量、小区产量和净光合速率可作为鉴定花生耐荫性的指标,可在相同条件下测定这5个指标,计算耐荫综合评价值预测花生耐荫性。

关键词: 花生, 耐荫性, 综合评价, 多元统计分析, 产量

Abstract:

【Objective】 The main aim of this study was to explore the methods of evaluating shade-tolerance, analyze the shade-tolerance of peanut in different genotypes, screen suitable identification indexes of shade tolerance, and establish an evaluation model of shade tolerance, so as to provide some theoretical support for screening of shade-tolerance resources and variety breeding of peanut. 【Method】 Qiannuo 868 was selected as the corn variety, and under the conditions of corn-peanut intercropping system and net cropping peanut, thirty peanut cultivars (lines) were treated in the field experiment. Net photosynthetic rate (X₁₃), stomatal conductance (X₁₄), intercellular CO₂ concentration (X₁₅) and transpiration rate (X₁₆) of peanut leaves were measured at the pod stage of peanut. The main stem height (X₁), side branch length (X₂), total branch number (X₃), effective branch number (X₄), plant pod number (X₅), mature pods per plant number (X₆) and yield per plant (X₁₁) were measured in the peanut mature stage. The 100-pod weight (X₇), kernel weight of 100 fruiting (X₈), 100-kernel weight (X₉), shelling percentage (X₁₀) and plot yield (X₁₂) were measured after harvesting and drying of peanut seed. 【Result】 The variation range of shade tolerance coefficient of each single index was different in different peanut varieties (lines). The 16 single indicators were converted into 5 independent comprehensive indicators through principal component analysis, and their contribution rates respectively were 33.860%, 26.666%, 11.176%, 8.471% and 6.954%, representing the information of 87.127% of all data. Through membership function, the largest membership function values of comprehensive indexes CI₁-CI₅respectively were 6-2, Tianfu 29, 201150118A, 201240413 and Minhua 6. The thirty peanut varieties (lines) were divided into 3 categories: the first category was shade tolerance type, including 11 varieties (lines); the second category was moderate shade tolerance type, including 18 varieties (lines); the third category was sensitive type, including 1 variety (line). The optimal mathematical model of peanut shade tolerance evaluation was established, namely D=0.741+0.576X₉+0.507X₁₁+ 0.298X₁₃+0.272X₁₂+0.406X₁₀ (R²=0.990), and its accuracy was higher than 93.18%. Then, 5 indexes for peanut shade tolerance identification were selected: 100-kernel weight, yield per plant, net photosynthetic rate, plot yield and the ratio of shelled. The shade tolerance type peanut had a higher net photosynthetic rate, higher 100-kernel weight, yield per plant, plot yield and shelling percentage, while the sensitive type peanut were the opposite. 【Conclusion】 It was relatively scientific to evaluate and analyze the shade tolerance of peanut by multivariate statistical analysis. 30 peanut varieties (lines) were divided into three categories: shade tolerance type, moderate shade tolerance type, and sensitive type. 100-kernel weight, shelling percentage, yield per plant, plot yield and net photosynthetic rate could be used as the indexes to identify the shade tolerance of peanut. The comprehensive evaluation value of shade tolerance could be calculated to predict peanut shade tolerance by measuring the five indexes under the same conditions.

Key words: peanut, shade-tolerance, comprehensive evaluation, multivariate statistical analysis, yield

胡廷会,成良强,王军,吕建伟,饶庆琳. 不同基因型花生耐荫性评价及其鉴定指标的筛选[J]. 中国农业科学, 2020, 53(6): 1140-1153.

TingHui HU,LiangQiang CHENG,Jun WANG,JianWei LÜ,QingLin RAO. Evaluation of Shade Tolerance of Peanut with Different Genotypes and Screening of Identification Indexes[J]. Scientia Agricultura Sinica, 2020, 53(6): 1140-1153.

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图/表 8

表1

参试材料信息"

材料 Material	品种类型 Cultivar type	选育单位 Breeding institute	生育期 Growth period (d)
201240401A	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	132
201060401C	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	134
201070406	普通型Virginia type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	130
2010605	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	130
201240413	普通型Virginia type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	133
201070407	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	132
徐花9号Xuhua 9	中间型Irregular type	江苏省徐州市农业科学院Institute of Agricultural Sciences of Xuzhou	129
201150119	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	130
花育21号Huayu 21	普通型Virginia type	山东省花生研究所Shandong Peanut Research Institute	133
201241001	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	132
天府29号Tianfu 29	珍珠豆型Spanish type	四川省南充市农业科学院Nanchong Institute of Agricultural Sciences	130
6-2	普通型Virginia type	山东省花生研究所Shandong Peanut Research Institute	128
201060105	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	133
201150104	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	130
冀花9号Jihua 9	普通型Virginia type	河北省农林科学院粮油作物研究所 Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences	129
花育33号Huayu 33	普通型Virginia type	山东省花生研究所Shangdong Peanut Research Institute	128
201060106B1	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	132
201150118A	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	130
徐花13号Xuhua 13	中间型Irregular type	江苏省徐州市农业科学院Institute of Agricultural Sciences of Xuzhou	128
天府22号Tianfu 22	中间型Irregular type	四川省南充市农业科学院Nanchong Institute of Agricultural Sciences	130
201150201	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	135
远杂6号Yuanza 6	珍珠豆型Spanish type	河南省农业科学院Henan Academy of Agricultural Sciences	131
闽花6号Minhua 6	珍珠豆型Spanish type	福建农林大学作物科学学院 College of Crop Science, Fujian Agriculture and Forestry University	129
天府18号Tianfu 18	普通型Virginia type	四川省南充市农业科学院Nanchong Institute of Agricultural Sciences	130
201150114A	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	131
中花6号Zhonghua 6	珍珠豆型Spanish type	中国农业科学院油料作物研究所 Oil Crops Research Institute, Chinese Academy of Agricultural Sciences	123
201240404	珍珠豆型Spanish type	贵州省农业科学院油料研究所 Oil Research Institute, Guizhou Academy of Agricultural Sciences	132
花育17号Huayu 17	普通型Virginia type	山东省花生研究所Shandong Peanut Research Institute	130
花育19号Huayu 19	普通型Virginia type	山东省花生研究所Shandong Peanut Research Institute	130
冀花10号Jihua 10	普通型Virginia type	河北省农林科学院粮油作物研究所 Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences	129

表1

表2

花生各单项指标的耐荫系数"

品种（系） Cultivar (Line)	X₁	X₂	X₃	X₄	X₅	X₆	X₇	X₈	X₉	X₁₀	X₁₁	X₁₂	X₁₃	X₁₄	X₁₅	X₁₆
6-2	1.04	0.89	0.60	0.51	0.48	0.47	0.77	0.64	0.94	0.83	0.41	0.40	1.06	1.85	1.73	0.92
花育17号Huayu 17	1.18	1.10	0.66	0.52	0.48	0.46	0.62	0.52	0.68	0.82	0.26	0.32	0.74	1.29	1.78	0.60
花育19号Huayu 19	0.98	0.93	0.72	0.63	0.50	0.48	0.75	0.59	0.81	0.79	0.37	0.28	0.82	1.30	1.66	0.75
花育21号Huayu 21	1.24	1.12	0.76	0.63	0.52	0.41	0.69	0.56	0.88	0.81	0.42	0.48	0.72	1.45	1.62	1.15
花育33号Huayu 33	1.16	1.01	0.76	0.63	0.47	0.51	0.58	0.55	0.78	0.93	0.43	0.36	0.72	1.26	1.98	0.90
冀花10号Jihua 10	1.03	0.89	0.71	0.56	0.43	0.39	0.65	0.58	0.82	0.89	0.32	0.28	0.80	1.51	2.14	0.83
冀花9号Jihua 9	1.08	0.94	0.72	0.57	0.51	0.55	0.59	0.51	0.72	0.82	0.28	0.32	0.68	1.25	1.61	0.52
闽花6号Minhua 6	1.15	0.99	0.61	0.51	0.31	0.30	0.52	0.49	0.68	0.94	0.28	0.32	0.79	1.48	3.56	0.97
天府18号Tianfu 18	0.97	0.77	0.77	0.53	0.39	0.32	0.60	0.51	0.69	0.85	0.32	0.30	0.64	1.29	3.52	1.74
天府22号Tianfu 22	1.06	0.70	0.71	0.57	0.34	0.39	0.78	0.67	0.94	0.85	0.40	0.36	0.95	1.65	2.73	1.22
天府29号Tianfu 29	0.97	0.79	0.72	0.49	0.39	0.37	0.81	0.69	0.96	0.86	0.44	0.36	0.97	2.02	2.80	1.26
徐花13号Xuhua 13	1	0.83	0.71	0.53	0.35	0.39	0.64	0.56	0.76	0.85	0.37	0.33	0.77	1.44	3.34	1.44
徐花9号Xuhua 9	1.06	0.80	0.73	0.58	0.40	0.38	0.70	0.61	0.87	0.87	0.40	0.46	0.83	1.51	3.16	1.13
远杂6号Yuanza 6	0.92	0.87	0.67	0.59	0.43	0.35	0.65	0.53	0.63	0.78	0.29	0.34	0.76	1.07	2.90	0.95
中花6号Zhonghua 6	0.93	0.78	0.59	0.45	0.30	0.28	0.57	0.48	0.60	0.81	0.28	0.31	0.73	1.33	3.40	1.27
201060105	1.05	0.83	0.74	0.63	0.58	0.58	0.62	0.54	0.74	0.87	0.36	0.35	0.69	1.23	1.57	1.02
201060106B1	0.89	0.75	0.79	0.66	0.52	0.52	0.75	0.68	0.90	0.91	0.37	0.46	0.86	1.40	2.03	0.91
201060401C	0.97	0.83	0.72	0.60	0.39	0.25	0.61	0.51	0.67	0.79	0.31	0.37	0.69	1.10	2.22	1.68
2010605	1.15	0.92	0.78	0.68	0.44	0.37	0.52	0.50	0.62	0.89	0.36	0.31	0.58	0.98	2.48	1.95
201070406	1.05	0.90	0.76	0.55	0.37	0.35	0.50	0.42	0.54	0.78	0.21	0.29	0.48	0.91	2.68	1.01
201070407	0.93	0.77	0.64	0.54	0.29	0.24	0.53	0.46	0.57	0.82	0.27	0.43	0.63	1.01	3.52	1.60
201150104	1.22	1.03	1.05	0.82	0.61	0.57	0.65	0.54	0.76	0.82	0.29	0.33	0.50	0.98	1.85	0.73
201150114A	0.9	0.73	0.73	0.55	0.33	0.30	0.59	0.51	0.66	0.83	0.29	0.27	0.72	1.20	2.74	1.17
201150118A	0.99	0.79	0.69	0.54	0.35	0.37	0.54	0.46	0.60	0.81	0.48	0.59	0.65	1.07	2.58	2.10
201150119	1.14	0.93	0.69	0.69	0.56	0.51	0.57	0.49	0.64	0.83	0.48	0.36	0.64	0.96	1.69	1.30
201150201	1.18	0.95	0.64	0.57	0.44	0.55	0.71	0.64	0.86	0.92	0.36	0.45	1.03	1.61	2.28	0.86
201240404	1.11	0.88	0.84	0.63	0.44	0.58	0.56	0.53	0.72	0.91	0.34	0.33	0.64	1.14	2.21	0.68
201241001	1.04	0.84	0.80	0.59	0.38	0.45	0.77	0.66	0.95	0.85	0.39	0.45	0.83	1.62	2.27	0.86
201240401A	1.07	0.96	0.85	0.76	0.56	0.64	0.70	0.61	0.81	0.87	0.40	0.59	0.70	1.17	2.14	1.18
201240413	1.42	1.03	0.74	0.61	0.44	0.49	0.71	0.56	0.81	0.79	0.47	0.40	0.76	1.50	2.15	1.00
平均值Average	1.06	0.88	0.73	0.59	0.43	0.43	0.64	0.55	0.75	0.85	0.35	0.37	0.75	1.32	2.41	1.12
标准差Stdev	0.18	0.15	0.12	0.12	0.12	0.15	0.10	0.09	0.14	0.06	0.11	0.12	0.19	0.38	0.77	0.50
变异系数CV(%)	17.00	17.72	16.03	20.39	30.47	36.56	22.38	23.10	19.92	7.05	29.76	33.71	29.06	28.99	29.38	40.84

表2

表3

表4

表5

各花生品种（系）耐荫性综合评价"

品种（系） Cultivar (Line)	CI₁	CI₂	CI₃	CI₄	CI₅	U(X₁)	U(X₂)	U(X₃)	U(X₄)	U(X₅)	D值 D value	综合评价 Comprehensive valuation
6-2	1.266	1.358	-0.700	0.767	-1.211	1.000	0.851	0.298	0.640	0.140	0.761	耐荫型 Shade tolerant
花育17号Huayu 17	0.021	-0.676	-2.157	0.988	-0.564	0.615	0.412	0.000	0.693	0.284	0.455	中度耐荫 Medium shade tolerant
花育19号Huayu 19	0.651	-0.123	-0.777	-0.750	-1.838	0.810	0.531	0.282	0.279	0.000	0.541	中度耐荫 Medium shade tolerant
花育21号Huayu 21	0.991	-0.646	0.222	1.771	-0.776	0.915	0.419	0.486	0.880	0.237	0.651	耐荫型Shade tolerant
花育33号Huayu 33	0.571	-0.687	-0.189	0.676	1.619	0.785	0.410	0.402	0.619	0.771	0.604	耐荫型Shade tolerant
冀花10号Jihua 10	0.193	0.405	-1.125	-0.537	0.536	0.668	0.645	0.211	0.330	0.530	0.559	中度耐荫 Medium shade tolerant
冀花9号Jihua 9	0.094	-0.865	-1.423	-0.260	-0.777	0.638	0.371	0.150	0.396	0.237	0.438	中度耐荫 Medium shade tolerant
闽花6号Minhua 6	-0.963	0.588	-1.535	1.162	2.644	0.311	0.685	0.127	0.735	1.000	0.498	中度耐荫 Medium shade tolerant
天府18号Tianfu 18	-1.158	0.423	0.580	-0.753	0.568	0.251	0.649	0.560	0.278	0.537	0.438	中度耐荫 Medium shade tolerant
天府22号Tianfu 22	0.683	1.596	0.416	-0.663	-0.096	0.820	0.902	0.526	0.300	0.389	0.722	耐荫型Shade tolerant
天府29号Tianfu 29	0.950	2.051	0.186	-0.322	-0.251	0.902	1.000	0.479	0.381	0.354	0.783	耐荫型Shade tolerant
徐花13号Xuhua 13	-0.489	0.822	0.253	-0.069	0.442	0.457	0.735	0.493	0.441	0.509	0.549	中度耐荫 Medium shade tolerant
徐花9号Xuhua 9	0.354	0.933	0.764	-0.034	0.616	0.718	0.759	0.597	0.450	0.547	0.675	耐荫型Shade tolerant
远杂6号Yuanza 6	-0.756	0.074	-0.397	-0.648	-1.339	0.375	0.574	0.360	0.303	0.111	0.406	中度耐荫 Medium shade tolerant
中花6号Zhonghua 6	-1.647	0.981	-0.639	0.116	-0.448	0.099	0.769	0.310	0.485	0.310	0.386	中度耐荫 Medium shade tolerant
201060105	0.420	-0.803	-0.031	-0.653	-0.017	0.738	0.385	0.435	0.302	0.406	0.522	中度耐荫 Medium shade tolerant
201060106B1	1.226	0.441	0.940	-1.920	0.521	0.988	0.653	0.633	0.000	0.526	0.707	耐荫型Shade tolerant
201060401C	-0.996	0.003	0.677	-0.105	-1.237	0.301	0.559	0.579	0.433	0.134	0.415	中度耐荫 Medium shade tolerant
2010605	-0.849	-1.023	0.939	0.304	1.367	0.346	0.337	0.633	0.530	0.715	0.428	中度耐荫 Medium shade tolerant
201070406	-1.735	-1.017	-0.922	-0.365	-0.645	0.072	0.339	0.253	0.371	0.266	0.221	敏感型 Shade susceptible
201070407	-1.968	0.405	0.717	0.045	-0.065	0.000	0.645	0.588	0.468	0.396	0.350	中度耐荫 Medium shade tolerant
201150104	0.784	-2.589	0.071	-1.614	-0.101	0.851	0.000	0.456	0.073	0.388	0.427	中度耐荫 Medium shade tolerant
201150114A	-1.171	0.441	-0.256	-1.276	-0.282	0.246	0.653	0.389	0.153	0.347	0.388	中度耐荫 Medium shade tolerant
201150118A	-1.068	0.106	2.734	1.762	-0.591	0.278	0.581	1.000	0.878	0.278	0.522	中度耐荫 Medium shade tolerant
201150119	0.061	-1.305	0.755	1.060	-0.596	0.627	0.277	0.596	0.710	0.277	0.496	中度耐荫 Medium shade tolerant
201150201	1.200	0.749	-0.692	0.867	1.246	0.980	0.720	0.300	0.664	0.688	0.759	耐荫型Shade tolerant
201240404	0.132	-0.944	-0.398	-0.894	1.628	0.649	0.354	0.360	0.245	0.773	0.492	中度耐荫 Medium shade tolerant
201241001	1.036	0.793	0.333	-0.630	-0.262	0.929	0.729	0.509	0.307	0.352	0.707	耐荫型Shade tolerant
201240401A	1.244	-1.063	1.814	-0.301	0.506	0.993	0.329	0.812	0.386	0.523	0.670	耐荫型Shade tolerant
201240413	0.923	-0.429	-0.161	2.275	-0.597	0.894	0.465	0.408	1.000	0.277	0.662	耐荫型Shade tolerant
权重Index weight						0.389	0.306	0.128	0.097	0.080

表5

图1

表6

表7

参考文献 33

[1]	林洪鑫, 潘晓华, 袁展汽, 肖运萍, 刘仁根, 汪瑞清, 吕丰娟 . 施氮和木薯-花生间作对木薯养分积累和系统养分利用的影响. 中国农业科学, 2018,51(17):3275-3290.
	LIN H X, PAN X H, YUAN Z Q, XIAO Y P, LIU R G, WANG R Q, LÜ F J . Effects of nitrogen application and cassava-peanut intercropping on cassava nutrient accumulation and system nutrient utilization. Scientia Agricultura Sinica, 2018,51(17):3275-3290. (in Chinese)
[2]	焦念元, 宁堂原, 赵春, 侯连涛, 李增嘉, 李友军, 付国占, 韩宾 . 施氮量和玉米-花生间作模式对氮磷吸收与利用的影响. 作物学报, 2008,34(4):706-712.
	JIAO N Y, NING T Y, ZHAO C, HOU L T, LI Z J, LI Y J, FU G Z, HAN B . Effects of nitrogen application and planting pattern on N and P absorption and use in maize-peanut intercropping system. Acta Agronomica Sinica, 2008,34(4):706-712. (in Chinese)
[3]	高砚亮, 孙占祥, 白伟, 冯良山, 杨宁, 蔡倩, 冯晨, 张哲 . 辽西半干旱区玉米与花生间作对土地生产力和水分利用效率的影响. 中国农业科学, 2017,50(19):3702-3713.
	GAO Y L, SUN Z X, BAI W, FENG L S, YANG N, CAI Q, FENG C, ZHANG Z . Productivity and water use efficiency of maize-peanut intercropping systems in the semi-arid region of western Liaoning province. Scientia Agricultura Sinica, 2017,50(19):3702-3713. (in Chinese)
[4]	ZUO Y M, ZHANG F S, LI X L, CAO Y P . Studies on the improvement in iron nutrition of peanut by intercropping with maize on a calcareous soil. Plant and Soil, 2000,220(12):13-25.
[5]	焦念元, 宁堂原, 杨萌珂, 付国占, 尹飞, 徐国伟, 李增嘉 . 玉米花生间作对玉米光合特性及产量形成的影响. 生态学报, 2013,33(14):4324-4330.
	JIAO N Y, NING T Y, YANG M K, FU G Z, YIN F, XU G W, LI Z J . Effects of maize-peanut intercropping on photosynthetic characters and yield forming of intercropped maize. Acta Ecologica Sinica, 2013,33(14):4324-4330. (in Chinese)
[6]	吴正锋, 刘俊华, 万书波, 孙奎香, 孙学武, 冯昊, 王才斌 . 遮光持续时间对花生荚果产量和品质的影响. 山东农业科学, 2011,2:30-33.
	WU Z F, LIU J H, WAN S B, SUN K X, SUN X W, FENG H, WANG C B . Effect of shading duration on pod yield and quality of peanut. Shandong Agricultural Sciences, 2011,2:30-33. (in Chinese)
[7]	焦念元, 汪江涛, 尹飞, 马超, 齐付国, 刘领, 付国占, 李友军 . 施用乙烯利和磷肥对玉米//花生间作氮吸收分配及间作优势的影响. 植物营养与肥料学报, 2016,22(6):1477-1484.
	JIAO N Y, WANG J T, YIN F, MA C, QI F G, LIU L, FU G Z, LI Y J . Effects of ethephon and phosphate fertilizer on N absorption and intercropped advantages of maize and peanut intercropping system. Journal of Plant Nutrition and Fertilizer, 2016,22(6):1477-1484. (in Chinese)
[8]	万书波, 郑亚萍, 刘道忠, 成波, 吴正峰, 陈殿绪, 王才斌 . 精播麦套花生套期、肥料与密度优化配置. 中国油料作物学报, 2006,28(3):319-323.
	WAN S B, ZHENG Y P, LIU D Z, CHENG B, WU Z F, CHEN D X, WANG C B . Optimization of peanut-wheat intercropping system on date, fertilizer and plant density. Chinese Journal of Oil Crop Sciences, 2006,28(3):319-323. (in Chinese)
[9]	张昆, 万勇善, 刘风珍 . 遮光对花生产量、成熟饱满度及品质的影响. 中国粮油学报, 2009,24(11):92-96.
	ZHANG K, WAN Y S, LIU F Z . Effects of shading on yield, plumpness and quality of peanut. Journal of the Chinese Cereals and Oils Association, 2009,24(11):92-96. (in Chinese)
[10]	焦念元, 陈明灿, 付国占, 宁堂原, 王黎明, 李增嘉 . 玉米花生间作复合群体的光合物质积累与叶面积指数变化. 作物杂志, 2007(1):34-35.
	JIAO N Y, CHEN M C, FU G Z, NING T Y, WANG L M, LI Z J . Studies on photosynthetic product accumulation and leaf area index in a complex population of companion cropping of corn and peanut. Crops, 2007(1):34-35. (in Chinese)
[11]	ZUO Y, ZHANG F . Effect of peanut mixed cropping with gramineous species on micronutrient concentrations and iron chlorosis of peanut plants grown in a calcareous soil. Plant and Soil, 2008,306(1):23-36.
[12]	吴正锋, 孙学武, 王才斌, 郑亚萍, 万书波, 刘俊华, 郑永美, 吴菊香, 冯昊, 于天一 . 弱光胁迫对花生功能叶片RuBP羧化酶活性及叶绿体超微结构的影响. 植物生态学报, 2014,38(7):740-748.
	WU Z F, SUN X W, WANG C B, ZHENG Y P, WAN S B, LIU J H, ZHENG Y M, WU J X, FENG H, YU T Y . Effects of low light stress on rubisco activity and the ultrastructure of chloroplast in functional leaves of peanut. Chinese Journal of Plant Ecology, 2014,38(7):740-748. (in Chinese)
[13]	孙艳, 高海顺, 管志勇, 陈素梅, 房伟民, 陈发棣 . 菊花近缘种属植物幼苗耐荫特性分析及其评价指标的确定. 生态学报, 2012,32(6):1908-1916.
	SUN Y, GAO H S, GUAN Z Y, CHEN S M, FANG W M, CHEN F D . Analysis of shade-tolerance and determination of evaluation indicators of shade-tolerance in seedlings of Chrysanthemum grandiflorum and its closely related genera. Acta Ecologica Sinica, 2012,32(6):1908-1916. (in Chinese)
[14]	梁颖, 李加纳 . 甘蓝型油菜耐荫性的品种差异. 作物学报, 2004,30(4):360-364.
	LIANG Y, LI J N . The varietal difference of tolerance to low light intensity in rape (Brassica napus L. ) plants. Acta Agronomica Sinica, 2004,30(4):360-364. (in Chinese)
[15]	袁刘正, 李潮海, 王秀萍, 杨世坤 . 不同玉米自交系耐荫性比较. 玉米科学, 2008,16(6):19-23.
	YUAN L Z, LI C H, WANG X P, YANG S K . Comparison of shade-tolerance among different maize (Zea mays L. ) inbred lines. Journal of Maize Sciences, 2008,16(6):19-23. (in Chinese)
[16]	武晓玲, 梁海媛, 杨峰, 刘卫国, 佘跃辉, 杨文钰 . 大豆苗期耐荫性综合评价及其鉴定指标的筛选. 中国农业科学, 2015,48(13):2497-2507.
	WU X L, LIANG H Y, YANG F, LIU W G, SHE Y H, YANG W Y . Comprehensive evaluation and screening identification indexes of shade tolerance at seedling in soybean. Scientia Agricultura Sinica, 2015,48(13):2497-2507. (in Chinese)
[17]	李春红, 姚兴东, 鞠宝韬, 朱明月, 王海英, 张惠君, 敖雪, 于翠梅, 谢甫绨, 宋书宏 . 不同基因型大豆耐荫性分析及其鉴定指标的筛选. 中国农业科学, 2014,47(15):2927-2939.
	LI C H, YAO X D, JU B T, ZHU M Y, WANG H Y, ZHANG H J, AO X, YU C M, XIE P T, SONG S H . Analysis of shade-toleranceand determination of shade-tolerance evaluation indicators in different soybean genotypes. Scientia Agricultura Sinica, 2014,47(15):2927-2939. (in Chinese)
[18]	赵银月, 詹和明, 代希茜, 单丹丹, 王铁军 . 云南间作大豆耐荫性综合评价及鉴定指标筛选. 中国油料作物学报, 2019,41(1):81-91.
	ZHAO Y Y, ZHAN H M, DAI X X, SHAN D D, WANG T J . Comprehensive evaluation and screening identification index of shade tolerance of intercropping soybean. Chinese Journal of Oil Crop Sciences, 2019,41(1):81-91. (in Chinese)
[19]	孙祖东, 张志鹏, 蔡昭艳, 曾维英, 赖振光, 陈怀珠, 杨守臻, 唐向民, 苏燕竹, 盖钧镒 . 大豆耐荫性评价体系的建立与中国南方大豆资源耐荫性变异. 中国农业科学, 2017,50(5):792-801.
	SUN Z D, ZHANG Z P, CAI Z Y, ZENG W Y, LAI Z G, CHEN H Z, YANG S Z, TANG X M, SU Y Z, GAI J Y . Establishment of an evaluation system of shade tolerance in soybean and its variation in southern China germplasm population. Scientia Agricultura Sinica, 2017,50(5):792-801. (in Chinese)
[20]	殷东生, 沈海龙 . 森林植物耐荫性及其形态和生理适应性研究进展. 应用生态学报, 2016,27(8):2687-2698.
	YIN D S, SHEN H L . Shade tolerance and the adaptability of forest plants in morphology and physiology: A review. Chinese Journal of Applied Ecology, 2016,27(8):2687-2698. (in Chinese)
[21]	孟庆立, 关周博, 冯佰利, 柴岩, 胡银岗 . 谷子抗旱相关性状的主成分与模糊聚类分析. 中国农业科学, 2009,42(8):2667-2675.
	MENG Q L, GUAN Z B, FENG B L, CHAI Y, HU Y G . Principal component analysis and fuzzy clustering on drought-tolerance related traits of foxtail millet (Setaria italica). Scientia Agricultura Sinica, 2009,42(8):2667-2675. (in Chinese)
[22]	戴海芳, 武辉, 阿曼古丽·买买提阿力, 王立红, 麦麦提·阿皮孜, 张巨松 . 不同基因型棉花苗期耐盐性分析及其鉴定指标筛选. 中国农业科学, 2014,47(7):1290-1300.
	DAI H F, WU H, AMANGULI M, WANG L H, MAIMAITI A, ZHANG J S . Analysis of salt-tolerance and determination of salt-tolerant evaluation indicators in cotton seedlings of different genotypes. Scientia Agricultura Sinica, 2014,47(7):1290-1300. (in Chinese)
[23]	武辉, 侯丽丽, 周艳飞, 范志超, 石俊毅, 阿丽艳·肉孜, 张巨松 . 不同棉花基因型幼苗耐寒性分析及其鉴定指标筛选. 中国农业科学, 2012,45(9):1703-1713.
	WU H, HOU L L, ZHOU Y F, FAN Z C, SHI J Y, ALlYAN R, ZHANG J S . Analysis of chilling-tolerance and determination of chilling-tolerance evaluation indicators in cotton of different genotypes. Scientia Agricultura Sinica, 2012,45(9):1703-1713. (in Chinese)
[24]	杨才琼, 胡宝予, 吴海军, 秦雯婷, 张潇文, 刘卫国, 杨文钰, 刘江 . 黑豆种质苗期耐荫性评价及其根系对弱光胁迫的响应. 中国生态农业学报, 2017,25(6):893-902.
	YANG C Q, HU B Y, WU H J, QIN W T, ZHANG X W, LIU W G, YANG W Y, LIU J . Evaluation for shade tolerance of black soybean germplasms and their root structure response to shade stress at seedling stage. Chinese Journal of Eco-Agriculture, 2017,25(6):893-902. (in Chinese)
[25]	LIU W G, REN M L, LIU T, DU Y L, ZHOU T, LIU X M, LIU J, HUSSAIN S, YANG W Y . Effect of shade stress on lignin biosynthesis in soybean stems. Journal of Integrative Agriculture, 2018,17(7):1594-1604.
[26]	LIU W G, SAJAD H, LIU T, ZOU J L, REN M L, ZHOU T, LIU J, YANG F, YANG W Y . Shade stress decreases stem strength of soybean through restraining lignin biosynthesis. Journal of Integrative Agriculture, 2017,18(1):43-53.
[27]	LIU W G, JIANG T, ZHOU X R, YANG W Y . Characterisitics of expansions in soybean ( Glycine max) internodes and responses to shade stress. Asian Journal of Crop Science, 2011,3(1):26-34.
[28]	刘婷, 刘卫国, 任梦露, 杜勇利, 邓榆川, 邹俊林, 方萍, 杨文钰 . 遮荫程度对不同耐荫性大豆品种光合及抗倒程度的影响. 中国农业科学, 2016,49(8):1466-1475.
	LIU T, LIU W G, REN M L, DU Y L, DENG Y C, ZOU J L, FANG P, YANG W Y . Effects of shade degrees on photosynthesis and lodging resistance degree of different shade tolerance soybean. Scientia Agricultura Sinica, 2016,49(8):1466-1475. (in Chinese)
[29]	罗玲, 于晓波, 万燕, 蒋涛, 杜俊波, 邹俊林, 杨文钰, 刘卫国 . 套作大豆苗期倒伏与茎秆内源赤霉素代谢的关系. 中国农业科学, 2015,48(13):2528-2537.
	LUO L, YU X B, WAN Y, JIANG T, DU J B, ZOU J L, YANG W Y, LIU W G . The relationship between lodging and stem endogenous gibberellins metabolism pathway of relay intercropping soybean at seedling stage. Scientia Agricultura Sinica, 2015,48(13):2528-2537. (in Chinese)
[30]	王在序, 盖树人 . 山东花生. 上海:上海科学技术出版社, 1999: 252-271.
	WANG Z X, GAI S R . Shandong Peanut. Shanghai:Shanghai Science and Technology Press, 1999: 252-271. (in Chinese)
[31]	贾士芳, 李从锋, 董树亭, 张吉旺 . 花后不同时期遮光对玉米粒重及品质影响的细胞学研究. 中国农业科学, 2010,43(5):911-921.
	JIA S F, LI C F, DONG S T, ZHANG J W . Effects of shading at different stages after anthesis on maize grain weight and quality at cytology level. Scientia Agricultura Sinica, 2010,43(5):911-921. (in Chinese)
[32]	陈勤操, 戴伟东, 蔺志远, 解东超, 吕美玲, 林智 . 代谢组学解析遮阴对茶叶主要品质成分的影响. 中国农业科学, 2019,52(6):1066-1077.
	CHEN Q C, DAI W D, LIN Z Y, XIE D C, LÜ M L, LIN Z . Effects of shading on main quality components in tea (Camellia Sinensis( L.) O. Kuntze) leaves based on metabolomics analysis. Scientia Agricultura Sinica, 2019,52(6):1066-1077. (in Chinese)
[33]	张昆, 万勇善, 刘风珍 . 苗期弱光对花生光合特性的影响. 中国农业科学, 2010,43(1):65-71.
	ZHANG K, WAN Y S, LIU F Z . Effects of weak light on photosynthetic characteristics of peanut seedlings. Scientia Agricultura Sinica, 2010,43(1):65-71. (in Chinese)

指标Index	X₁	X₂	X₃	X₄	X₅	X₆	X₇	X₈	X₉	X₁₀	X₁₁	X₁₂	X₁₃	X₁₄	X₁₅	X₁₆
X₁	1
X₂	0.799**	1
X₃	0.245	0.178	1
X₄	0.357	0.356	0.789**	1
X₅	0.398*	0.525**	0.520**	0.753**	1
X₆	0.455*	0.429*	0.487**	0.621**	0.827**	1
X₇	0.008	-0.089	0.079	0.053	0.236	0.303	1
X₈	0.000	-0.129	0.118	0.095	0.235	0.380*	0.935**	1
X₉	0.168	0.024	0.151	0.088	0.275	0.403*	0.914**	0.951**	1
X₁₀	0.089	-0.009	0.062	0.083	0.035	0.262	-0.002	0.330	0.289	1
X₁₁	0.294	0.035	0.030	0.210	0.253	0.340	0.449*	0.455*	0.511**	0.111	1
X₁₂	0.085	0.010	0.067	0.224	0.129	0.252	0.261	0.290	0.288	0.062	0.547**	1
X₁₃	-0.106	-0.178	-0.478**	-0.397*	-0.107	0.062	0.762**	0.791**	0.745**	0.263	0.355	0.175	1
X₁₄	0.037	-0.102	-0.313	-0.457*	-0.154	0.016	0.753**	0.770**	0.815**	0.263	0.356	0.099	0.878**	1
X₁₅	-0.406*	-0.522**	-0.312	-0.522**	-0.827**	-0.719**	-0.269	-0.230	-0.316	0.098	-0.294	-0.099	-0.030	0.041	1
X₁₆	-0.282	-0.440*	-0.147	-0.124	-0.429*	-0.554**	-0.309	-0.319	-0.371*	-0.121	0.230	0.259	-0.243	-0.239	0.476**	1

主成分Principle factor		CI₁	CI₂	CI₃	CI₄	CI₅
特征向量Eigenvector	X₁	0.413	-0.477	-0.201	0.587*	0.203
	X₂	0.331	-0.598*	-0.400	0.505	0.054
	X₃	0.310	-0.614*	0.283	-0.473	0.113
	X₄	0.408	-0.746*	0.353	-0.238	0.063
	X₅	0.645	-0.646*	0.000	-0.105	-0.140
	X₆	0.751*	-0.489	-0.015	-0.081	0.100
	X₇	0.792*	0.462	0.048	-0.164	-0.281
	X₈	0.831*	0.474	0.077	-0.232	0.031
	X₉	0.879*	0.406	0.013	-0.092	0.016
	X₁₀	0.274	0.132	-0.015	-0.110	0.911*
	X₁₁	0.569*	0.136	0.550	0.426	-0.030
	X₁₂	0.347	0.062	0.680*	0.323	0.005
	X₁₃	0.554	0.768*	-0.164	0.088	-0.021
	X₁₄	0.556	0.751*	-0.208	0.120	0.056
	X₁₅	-.638*	0.512	0.132	-0.059	0.327
	X₁₆	-0.514	0.181	0.722*	0.216	0.017
特征值Eigenvalue		5.418	4.267	1.788	1.355	1.113
贡献率Contribution rate		33.860	26.666	11.176	8.471	6.954
累计贡献率Cumulative contribution rate		33.860	60.526	71.702	80.173	87.127

品种（系） Cultivar (Line)	D值 D value	回归值 Regression	拟合误差 Fitting error	估计精度 Evaluation accuracy (%)
6-2	0.7606	0.7688	-0.0081	98.93
花育17号Huayu 17	0.4553	0.4243	0.0310	93.18
花育19号Huayu 19	0.5407	0.5541	-0.0133	97.53
花育21号Huayu 21	0.6506	0.6570	-0.0064	99.02
花育33号Huayu 33	0.6039	0.6181	-0.0142	97.65
冀花10号Jihua 10	0.5586	0.5693	-0.0107	98.08
冀花9号Jihua 9	0.4381	0.4374	0.0007	99.85
闽花6号Minhua 6	0.4979	0.4912	0.0067	98.66
天府18号Tianfu 18	0.4377	0.4346	0.0031	99.30
天府22号Tianfu 22	0.7222	0.7290	-0.0067	99.07
天府29号Tianfu 29	0.7835	0.7663	0.0172	97.81
徐花13号Xuhua 13	0.5494	0.5482	0.0012	99.79
徐花9号Xuhua 9	0.6754	0.6868	-0.0114	98.32
远杂6号Yuanza 6	0.4059	0.4081	-0.0022	99.46
中花6号Zhonghua 6	0.3858	0.3790	0.0068	98.23
201060105	0.5223	0.5177	0.0046	99.11
201060106B1	0.7070	0.7194	-0.0124	98.24
201060401C	0.4149	0.4279	-0.0130	96.87
2010605	0.4276	0.4122	0.0153	96.41
201070406	0.2214	0.2179	0.0035	98.43
201070407	0.3500	0.3595	-0.0095	97.29
201150104	0.4272	0.4189	0.0082	98.07
201150114A	0.3881	0.4101	-0.0220	94.33
201150118A	0.5217	0.5323	-0.0106	97.98
201150119	0.4961	0.4999	-0.0038	99.23
201150201	0.7589	0.7374	0.0216	97.16
201240404	0.4925	0.4974	-0.0049	99.00
201241001	0.7074	0.7133	-0.0058	99.17
201240401A	0.6700	0.6539	0.0161	97.59
201240413	0.6615	0.6225	0.0391	94.10

类别 Cluster	正常光照CK					遮荫Shade
类别 Cluster	X₉	X₁₁	X₁₃	X₁₂	X₁₀	X₉	X₁₁	X₁₃	X₁₂	X₁₀
第一类 First cluster	48.59	15.05	18.15	308.18	0.62	43.43	5.89	10.45	126.89	0.54
第二类 Second cluster	55.40	14.92	18.22	296.86	0.63	35.17	4.87	10.91	100.05	0.53
第三类 Third cluster	63.38	17.29	17.12	305.41	0.63	26.80	3.80	9.34	88.55	0.49