中国农业科学 ›› 2019, Vol. 52 ›› Issue (22): 4154-4165.doi: 10.3864/j.issn.0578-1752.2019.22.019

• 栽培生理 • 上一篇    下一篇

播期对糜子农艺性状及淀粉理化性质的影响

罗艳1,屈洋2,杨清华1,张伟丽1,宫香伟1,李境1,高小丽1,高金锋1,杨璞1,王鹏科1,冯佰利1()   

  1. 1 西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室/农业部作物基因资源与种质创制陕西科学观测实验站,陕西杨凌 712100
    2 宝鸡市农业科学院,陕西宝鸡 722400
  • 收稿日期:2019-06-14 接受日期:2019-09-12 出版日期:2019-11-16 发布日期:2019-11-16
  • 通讯作者: 冯佰利
  • 作者简介:罗艳,E-mail:18220813910@163.com
  • 基金资助:
    国家谷子高粱产业技术体系(CARS-13.5-06-A26);国家“十二五”科技支撑计划(2014BAD07B03);国家自然科学基金(31371529);陕西省2017年省级现代农作物种业项目(20171010000004);陕西省小杂粮产业技术体系项目(NYKJ-2018-YL19)

Effect of Sowing Date on Agronomic Traits and Starch Physicochemical Properties of Proso Millet

LUO Yan1,QU Yang2,YANG QingHua1,ZHANG WeiLi1,GONG XiangWei1,LI Jing1,GAO XiaoLi1,GAO JinFeng1,YANG Pu1,WANG PengKe1,FENG BaiLi1()   

  1. 1 College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Research Station of Crop Gene Resources & Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, Shaanxi;
    2 Baoji Academy of Agricultural Sciences, Baoji 722400, Shaanxi
  • Received:2019-06-14 Accepted:2019-09-12 Online:2019-11-16 Published:2019-11-16
  • Contact: BaiLi FENG

摘要:

【目的】分析不同播期对糜子籽粒性状、农艺性状和籽粒淀粉理化性质的影响,为后续机理研究提供依据。【方法】 采用盆栽试验,以陕糜1号为试验材料,设置4月20日(B1)、5月10日(B2)、5月30日(B3)、6月20日(B4)4个播期,全生育期保持土壤充足供水。系统研究糜子成熟期农艺性状指标、籽粒性状及籽粒淀粉理化性质,分析不同播期下糜子株高、穗长、穗柄长、穗分枝数、千粒重、籽粒长/宽/圆度等农艺性状指标,研究淀粉晶体结构,粒径分布、直链淀粉含量以及淀粉的热焓特性及糊化特性的变化,总结淀粉的直链淀粉含量、热焓特性及糊化特性与形态指标、粒径分布及粒径参数的相关性。【结果】 随着播期的推迟,糜子的株高、穗长、穗柄长极显著增加,主茎分蘖、分枝显著减少,穗二级分枝数和籽粒粒宽显著降低。籽粒淀粉粒径分布均呈双峰分布,且与其他播期相比,B1播期下粒径>28 μm颗粒的比例最高(25.5%),5—28 μm颗粒的比例最低(67.5%)。播期不影响糜子淀粉的晶体类型,但是影响了X-射线衍射图谱的衍射峰强度。B1播期的直链淀粉含量(1.3%)极显著高于其余播期处理(0.18%—0.53%)。不同播期的籽粒淀粉的热焓特性相关指标中,糊化起始温度(To)、峰值温度(Tp)和终值温度(Tc)整体呈现出先增加后降低的趋势,而热焓值(ΔH)没有显著差异。不同播期下的糜子淀粉糊化特性各项指标均呈现显著性差异,其中,B3的峰值黏度(Pv)、谷值黏度(Tv)、最终黏度(Fv)和破损值(BD)显著高于其余处理,而回生值(SB)及糊化温度(PT)显著低于其他处理。随着播期的推迟,糜子淀粉的峰值黏度和破损值呈现先增加再减少的趋势,B1淀粉的热糊稳定性最强,B3淀粉的冷糊稳定性最强,但热糊稳定性最差,B2淀粉的冷糊稳定性最差,B4的冷糊、热糊稳定性均处于中间水平。【结论】 适宜的播期可以提供充分满足糜子生长需要的光、温条件。早播使糜子籽粒千粒重显著增加,籽粒更加饱满,且籽粒淀粉的平均粒径增加,具有较高的直链淀粉含量,更加适合加工热食,具有更高的营养价值。播期不影响淀粉的晶体类型,但是影响淀粉的X-射线的衍射峰强度,也会影响糊化特性等指标。

关键词: 播期, 糜子, 淀粉, 糊化特性, 晶体结构

Abstract:

【Objective】 The purpose of this study was to analyze the effects of different sowing date on proso millet (Panicum miliaceum L.) agronomic traits and grain starch physiochemical properties, provide theoretical basis for the subsequent study of the mechanism of sowing date on proso millet.【Method】The experiment was carried out in a pot, and Shaanmei No. 1 was used as the test material. Four kinds of sowing dates were set on April 20 (B1), May 10 (B2), May 30 (B3), and June 20 (B4). Different sowing dates, the soil moisture was sufficient during the whole growth period. In 2017, the morphological indexes, grain shape and grain physicochemical properties of the seeds of the proso millet were studied. The plant height, ear length, ear stem length, number of ear branches, 1 000-grain weight, the seeds length, width and roundness of proso millet were analyzed under different sowing dates. In the meantime, the crystal structure, particle size distribution, amylose content, and the enthalpy and gelatinization properties of starch were studied. The correlation of amylose content, enthalpy characteristics and gelatinization properties of starch with morphological index, particle size distribution and particle size parameters were analyzed. 【Result】 The results showed that with the delay of sowing date, the plant height, ear length and ear stem length of proso millet were significantly increased, the main stem tillers and branches were significantly decreased, and the number of secondary spike branches and grain width were significantly decreased. The size distribution of grain starch granules showed a bimodal distribution, and compared with other sowing dates, the particles proportion of B1 had the highest > 28 μm (25.5%) and the lowest 5-28 μm (67.5%) starch granule. The X-ray diffraction pattern showed that changing the sowing date couldn’t affect the crystal type of the proso millet starch, but affected the diffraction peak intensity of the X-ray diffraction pattern. The amylose content (1.3%) of B1 was significantly higher than that in the other sowing dates (0.18%-0.53%). Among the indicators related to the enthalpy characteristics of grain starch in different sowing dates, the gelatinization onset temperature (To), peak temperature (Tp) and conclusion temperature (Tc) showed a tendency of increasing first and then decreasing, while the thermal enthalpy (ΔH) had no significant difference. There were significant differences in the gelatinization properties of proso millet starch under different sowing dates. The peak viscosity (Pv), through viscosity (Tv), final viscosity (Fv) and breakdown (BD) of B3 were significantly higher than the others, while the setback (SB) and peak temperature (PT) were significantly lower than other treatments. With the delay of sowing date, the Pv and BD of proso millet starch increased first and then decreased. B1 starch had the strongest hot paste stability, B3 starch had the strongest cold paste stability and the worst hot paste stability. The cold paste stability of B2 starch was the worst, while the cold paste and hot paste stability of B4 were at an intermediate level. 【Conclusion】 Appropriate sowing date could provide sufficient light and temperature conditions to provide a better environment for the growth of proso millet. Early sowing date significantly increased the 1 000-grain weight of proso millet kernels, the grain was fuller, and the average grain size of grain starch increased, with higher amylose content and nutritional value, which was more suitable for processing hot foods. The sowing date didn’t affect the crystal type of starch, but affected the intensity of the X-ray diffraction peak, the gelatinization characteristics and other indicators of the starch.

Key words: sowing date, Panicum miliaceum L., starch, gelatinization characteristics, crystal structure