薇甘菊光能利用及叶绿素合成在不同光照强度下的响应

doi:10.3864/j.issn.0578-1752.2022.12.007

中国农业科学 ›› 2022, Vol. 55 ›› Issue (12): 2347-2359.doi: 10.3864/j.issn.0578-1752.2022.12.007

薇甘菊光能利用及叶绿素合成在不同光照强度下的响应

金梦娇^1,²(),刘博²(),王抗抗²,张广忠²,钱万强²(),万方浩^1,²()

¹青岛农业大学植物医学学院,山东青岛 266109
²中国农业科学院深圳农业基因组研究所/岭南现代农业科学与技术广东省实验室深圳分中心/农业农村部农业基因数据分析重点实验室,广东深圳 518120

收稿日期:2021-12-03 接受日期:2021-12-27 出版日期:2022-06-16 发布日期:2022-06-23
通讯作者: 钱万强,万方浩
作者简介:金梦娇,E-mail： jinmj_97@163.com。|刘博,E-mail： liubo03@caas.cn。
基金资助:
国家自然科学基金面上项目(32072490)

Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha

JIN MengJiao^1,²(),LIU Bo²(),WANG KangKang²,ZHANG GuangZhong²,QIAN WanQiang²(),WAN FangHao^1,²()

¹College of Plant Medicine, Qingdao Agricultural University, Qingdao 266109, Shandong
²Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences/Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture/Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Shenzhen 518120, Guangdong

Received:2021-12-03 Accepted:2021-12-27 Online:2022-06-16 Published:2022-06-23
Contact: WanQiang QIAN,FangHao WAN

摘要/Abstract

摘要：

【目的】光是植物进行光合作用的重要生态因素之一,光合色素对光的捕获和利用影响植物的生长发育进程,进而影响其在自然生态系统中的生存和适合度。明确薇甘菊（Mikania micrantha）光合生理特征与叶绿素生物合成途径的基因表达对不同光照强度的响应,以及薇甘菊光合能量与叶绿素转化的相互关系,为解析薇甘菊“快速生长”提供生理生态学证据。【方法】以薇甘菊为研究对象,采用乙醇浸提法测定不同光照强度（0、20%、40%、100%）下各光合色素含量,分析其叶绿素a和b比值（Chl a/b）的变化规律,比较不同光合途径代表植物（C3、C4和CAM）光合特性;利用微量法和蒽酮比色法分别测定上述光照强度下薇甘菊叶片组织中ATP和淀粉含量;构建不同光照强度下薇甘菊cDNA文库并开展转录组测序;利用OrthoFinder、Blastp、HISAT2、StringTie和R包等生物信息学软件分析不同光照强度变化下薇甘菊叶绿素合成和捕光复合体（LHC）基因表达模式,阐述叶绿素合成途径相关基因表达变化规律。【结果】在100%的光照强度下,薇甘菊叶绿素b和类胡萝卜素含量以及Chl a/b与C4植物玉米相近,并且薇甘菊和玉米叶片中Chl a/b显著高于C3（水稻和番茄）和CAM（芦荟）植物。不同光照强度下的薇甘菊叶片中叶绿素a、叶绿素b和类胡萝卜素含量变化不显著,但Chl a/b呈现出随光照强度增加而显著增加的趋势;40%和100%光照强度下,薇甘菊叶片中ATP含量变化幅度较小,而淀粉含量随光照强度上升显著升高;当光照强度为0时淀粉含量急剧下降,此时ATP含量呈现升高趋势;薇甘菊叶绿素生物合成中HEMA、CHLH、CRD1和CAO基因家族的基因表达量受光诱导调控,高光照强度下捕光复合体（LHC）基因的表达量较高。【结论】不同光照强度下薇甘菊叶片可能通过调节叶绿素a和b的合成,调控淀粉和ATP的相互转化,奠定了薇甘菊较高光合速率和较强光适应能力的基础。

关键词: 薇甘菊, 光合色素特征, ATP含量, 淀粉储存, 不同光环境的响应, 基因表达调控

Abstract:

【Objective】Light is one of the important ecological factors for photosynthesis of plants. The capture and utilization of light by photosynthetic pigments affects the growth and development of plants, and then affects their survival and fitness in natural ecosystems. The objective of this study is to clarify the photosynthetic physiological characteristics and response of gene expression of chlorophyll biosynthesis pathway to different light intensities in Mikania micrantha, as well as the relationship between photosynthetic energy and chlorophyll transformation, and to provide physiological and ecological evidences for explaining the “rapid growth” of M. micrantha.【Method】The photosynthetic pigment content of M. micrantha was determined by ethanol extraction under different light intensities (0, 20%, 40% and 100%). The variation pattern of chlorophyll a and b ratio (Chl a/b) was analyzed. The photosynthetic characteristics of M. micrantha were compared with the representative plants of different photosynthetic pathways (C3, C4 and CAM). The contents of ATP and starch in M. micrantha leaves under the above light intensities were determined by micro-method and anthrone colorimetric method. The cDNA library of M. micrantha under different light intensities was established and sequenced. Bioinformatics software including OrthoFinder, Blastp, HISAT2, StringTie, R package were used to analyze the expression pattern of genes involved in chlorophyll synthesis and light-harvesting complex (LHC) in M. micrantha under different light intensities. The expression patterns of genes related to chlorophyll synthesis pathway were identified.【Result】At 100% light intensity, the Chl b and carotenoid contents and Chl a/b in M. micrantha leaves were similar to those in C4 plant (maize). The Chl a/b in M. micrantha and maize leaves was significantly higher than that in C3 (rice and tomato) and CAM (aloe) plants. The contents of Chl a, Chl b and carotenoid in M. micrantha leaves under different light intensities did not change significantly, but Chl a/b significantly increased with increasing light intensity. Between the 40% and 100% light intensity, ATP content in M. micrantha leaves changed slightly, while starch content in M. micrantha leaves increased significantly in 100% light intensity. When the light intensity was 0, starch content decreased sharply and ATP content increased. The gene expression levels of HEMA, CHLH, CRD1 and CAO gene families involved in chlorophyll biosynthesis in M. micrantha were regulated by light induction, and the expression levels of light-harvesting complex (LHC) genes were higher under high light intensity.【Conclusion】Under different light conditions, M. micrantha may regulate the synthesis of Chl a and Chl b, and the mutual transformation of starch and ATP, which lay the foundation for the higher photosynthetic rate and light adaptability of M. micrantha.

Key words: Mikania micrantha, photosynthetic pigment characteristics, ATP content, starch storage, response to different light environments, regulation of gene expression

金梦娇,刘博,王抗抗,张广忠,钱万强,万方浩. 薇甘菊光能利用及叶绿素合成在不同光照强度下的响应[J]. 中国农业科学, 2022, 55(12): 2347-2359.

JIN MengJiao,LIU Bo,WANG KangKang,ZHANG GuangZhong,QIAN WanQiang,WAN FangHao. Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha[J]. Scientia Agricultura Sinica, 2022, 55(12): 2347-2359.

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基因家族 Gene family	基因名称 Gene ID	不同时间下的光照强度 Light intensity under different times
基因家族 Gene family	基因名称 Gene ID	8：00 (40%)	12：00 (100%)	17：00 (20%)	18：00 (0)
HEMA	Mm03G008458	265.7	3069.0	516.0	505.3
	Mm04G009926	1.0	8.3	19.0	12.3
	Mm04G009947	0.3	9.0	29.3	6.3
	Mm09G022271	1165.3	796.3	950.0	1128.7
HEMC	Mm03G007750	1651.0	1452.3	2872.0	2340.3
	Mm15G034118	326.0	279.7	741.3	636.0
	Mm15G034127	309.7	283.0	774.3	608.0
HEMD	Mm19G040701	549.0	542.0	628.0	722.0
	Mm19G040704	292.67	181.0	282.7	359.3
	Mm19G040708	207.3	107.0	118.3	147.3
	Mm19G040711	142.3	86.3	79.3	104.7
HEME	Mm01G003424	1007.7	810.3	2560.0	2159.0
	Mm07G017442	324.3	427.7	1019.3	875.7
	Mm14G033554	1.3	3.7	5.3	7.0
HEMF	Mm04G008958	4639.3	3728.0	6053.0	5493.3
GUN4	Mm06G015460	11.0	20.0	25.0	13.3
GUN4	Mm06G015530	6.0	12.7	14.7	13.0
CHLH	Mm13G030916	9896.7	15342.3	2337.0	2011.3
CHLH	MmUnG043782	6985.3	10106.3	1522.3	1202.3
CHLI	Mm18G039053	2991.7	5393.0	5682.7	5434.0
CHLI	Mm01G002914	3245.3	2445.0	3985.0	3772.0
CRD1	Mm13G030861	2430.3	2990.7	423.3	386.7
CRD1	Mm13G030884	3649.3	4209.0	594.7	483.3
PCB	Mm17G037161	216.0	472.3	710.7	513.0
POR	Mm01G002138	814.0	3970.3	5360.0	4283.0
	Mm03G008274	32.0	1323.7	3327.3	4707.0
	Mm04G009104	28.7	73.0	117.3	94.0
	Mm07G016864	12.7	40.7	104.7	113.0
	Mm16G036000	16.3	12.3	45.0	17.0
	MmUnG042187	17.7	14.0	60.3	26.7
	MmUnG044337	0.3	0.7	1.3	0.3
G4	Mm07G017733	17.3	4.0	34.7	14.7
G4	Mm17G037705	3177.7	2369.0	3596.0	3518.0
CLH	Mm06G015923	107.0	116.3	276.7	51.3
	Mm07G018083	430.0	857.3	966.7	785.0
	MmUnG042738	1.7	2.7	5.3	2.0
CAO	Mm01G001296	67.3	276.3	165.7	63.0
	Mm01G001298	2.0	3.3	4.0	1.3
	Mm02G004540	5003.7	2837.0	421.0	518.3
	Mm05G012908	4965.3	4202.7	2773.3	2253.7
	Mm08G021299	1425.3	2045.7	549.3	573.0
	Mm16G036155	706.7	757.7	1119.3	911.7

基因家族 Gene family	基因名称 Gene ID	不同时间下的光照强度 Light intensity under different times
基因家族 Gene family	基因名称 Gene ID	8：00 (40%)	12：00 (100%)	17：00 (20%)	18：00 (0)
LHCB2	Mm01G002159	182.0	4514.0	138.0	174.0
	Mm01G002160	15.0	689.0	20.7	109.7
	Mm07G019027	6013.7	27098.0	1747.7	1522.7
	Mm18G039443	8876.3	79479.0	15727.0	5623.7
	MmUnG042961	24.0	873.7	50.7	130.7
	MmUnG042962	1025.0	9108.3	427.0	466.7
	Mm11G026494	660.7	8919.0	3085.7	3943.7
LHCB3	Mm16G035290	425.0	23868.7	6085.0	5605.7
	MmUnG044047	7463.0	36412.7	3480.3	6033.0
	Mm12G028787	713.7	5819.0	784.3	1731.0
	Mm12G028786	33.0	109.3	57.0	64.0
	Mm07G019026	4625.3	15617.3	2046.7	2507.7
LHCB5	Mm19G040589	6216.3	24846.7	9970.7	9191.3
	Mm11G026495	2.0	183.7	54.7	270.3
	Mm11G026493	246.3	3081.3	1335.7	2221.0
	Mm10G024129	593.3	12951.7	1605.0	784.7
	Mm11G026496	264.0	6615.3	1325.7	2067.3
LIL3	Mm04G009004	3726.0	2896.0	5061.7	4622.0
LIL3	Mm12G028071	1768.3	1584.3	2006.3	1737.0

薇甘菊光能利用及叶绿素合成在不同光照强度下的响应

Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha

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