长期施肥旱地土壤易分解与耐分解氮的矿化特性

doi:10.3864/j.issn.0578-1752.2015.23.011

中国农业科学 ›› 2015, Vol. 48 ›› Issue (23): 4698-4706.doi: 10.3864/j.issn.0578-1752.2015.23.011

• 土壤肥料·节水灌溉·农业生态环境 • 上一篇下一篇

长期施肥旱地土壤易分解与耐分解氮的矿化特性

武红亮¹，于维水¹，朱平²，张水清³，赵雅雯¹，刘婧¹，王士超¹，孟繁华⁴，卢昌艾¹

¹中国农业科学院农业资源与农业区划研究所/耕地培育技术国家工程实验室，北京100081
²吉林省农业科学院农业资源与环境研究所，长春 130124
³河南省农业科学院植物营养与资源环境研究所，郑州 450002
⁴河南省土壤肥料工作站，郑州450002

收稿日期:2015-09-16 出版日期:2015-12-01 发布日期:2015-12-01
通讯作者: 卢昌艾，E-mail：luchangai@caas.cn
作者简介:武红亮，E-mail：971158093@qq.com
基金资助:
国家科技支撑计划（2012BAD05B05）、国家“973”计划（2013CB127404）、国家公益性行业（农业）科研专项（201203030）

Mineralization Characteristics of Upland Soil Labile and Recalcitrant Nitrogen Under Long-Term Different Fertilization Systems

WU Hong-liang¹, YU Wei-shui¹, ZHU Ping², ZHANG Shui-qing³, ZHAO Ya-wen¹, LIU Jing¹, WANG Shi-chao¹, MENG Fan-hua⁴, LU Chang-ai¹

¹Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable Land, Beijing 100081
²Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun 130124
³Institute of Plant Nutrition and Environmental Resources, Henan Academy of Agricultural Sciences, Zhengzhou 450002
⁴Henan Soil and Fertilizer Station, Zhengzhou 450002

Received:2015-09-16 Online:2015-12-01 Published:2015-12-01

摘要/Abstract

摘要： 【目的】土壤易分解氮库和耐分解氮库是土壤有机质的重要组分，其矿化能力的大小可反映土壤有机氮的周转性能。论文旨在研究长期不同施肥制度下土壤易分解氮库与耐分解氮库的矿化特性，为了解不同培肥措施及其氮素供应提供依据。【方法】以中国长期不同施肥处理的2种旱地土壤（黑土和潮土）为例，选取不施肥(CK)、单施化肥(NPK)、化肥配施秸秆(NPKS)和化肥配施有机肥(NPKM)4个处理，采用颗粒密度法，将土壤有机氮分为易分解氮和耐分解氮2个组分，室内培养分析不同组分氮的矿化特性。【结果】筛分及培养结果显示，黑土和潮土的平均质量回收率和氮回收率均超过97%，易分解和耐分解氮组分矿化量之和占原土矿化量的平均比例为99.91%（99.89%—99.93%），是一种研究土壤易分解和耐分解氮组分矿化特性的可行方法。2种旱地土壤NPK、NPKS和NPKM处理易分解氮组分净氮矿化潜势（除黑土NPK处理差异不显著）较CK处理显著提高26.82%—137.10%；不同施肥处理对旱地黑土、潮土易分解氮组分净氮矿化潜势影响显著，其中，黑土NPKM处理易分解氮组分净氮矿化潜势为1.48 mg?kg^-1?d^-1，显著优于NPKS（1.02 mg?kg^-1?d^-1）与NPK（0.75 mg?kg^-1?d^-1）处理；潮土NPKM处理易分解氮组分净氮矿化潜势为1.17 mg?kg^-1?d^-1，显著优于NPKS（0.89 mg?kg^-1?d^-1）与NPK（0.76 mg?kg^-1?d^-1）处理；旱地土壤各处理耐分解氮组分净氮矿化潜势之间差异不显著，其中，黑土各处理耐分解氮组分平均净氮矿化潜势为0.58 mg?kg^-1?d^-1（0.52—0.63 mg?kg^-1?d^-1），潮土为0.51 mg?kg^-1?d^-1（0.40—0.62 mg?kg^-1?d^-1）。不同施肥处理旱地黑土、潮土易分解氮组分净氮矿化潜势均显著大于同处理耐分解氮组分净氮矿化潜势，NPKM处理两者显现出最大差异，其中，黑土易分解氮组分净氮矿化潜势是同处理（按CK、NPK、NPKS、NPKM顺序）耐分解氮组分的1.41、1.39、1.75和2.35倍，潮土易分解氮组分净氮矿化潜势是同处理（按CK、NPK、NPKS、NPKM顺序）耐分解氮组分的1.22、1.33、1.56和1.87倍。土壤矿化过程中易分解组分对原土矿化贡献率受施肥措施显著影响，其大小按CK、NPK顺序递增，黑土依次为10.82%、12.51%、14.94%、18.91%，潮土依次为8.49%、9.71%、11.34%、16.2%；旱地土壤各处理耐分解氮组分的矿化贡献率之间无显著差异，黑土为86.24%（83.96%—88.51%），潮土为88.46%（86.71%—90.20%）。【结论】旱地易分解氮组分净氮矿化潜势和矿化贡献率对不同施肥措施的反应较耐分解氮组分敏感，各施肥处理易分解氮组分净氮矿化潜势显著高于耐分解组分，NPKM处理两组分矿化潜势差异最大，且对氮素矿化潜势和矿化贡献率的提高优于NPKS处理，更优于NPK处理。

关键词: 易分解氮, 耐分解氮, 黑土, 潮土, 矿化特性, 长期施肥

Abstract: 【Objective】Labile nitrogen (Lab-N) and recalcitrant nitrogen (Rec-N) are important components of soil organic matter, and their mineralization ability has an effect on organic nitrogen turnover properties. The objective of this study is to research the mineralization characteristics of soil labile and recalcitrant nitrogen under different long-term fertilization, and to provide a basis for different fertilization measures and their nitrogen supplying capacities. 【Method】Using laboratory incubation method, soil labile and recalcitrant nitrogen fractions separated by particle size-density separation method were studied under four treatments, namely no fertilizer (CK), chemical fertilizer (NPK), chemical fertilizer combined with straw (NPKS), and chemical fertilizer combined with manure (NPKM) from two typical long-term experiment sites (black soil and fluvo-aquic soil) in China. 【Result】Results of laboratory sieving and incubation showed that the method was simple and suitable to study the mineralization characteristics of soil labile and recalcitrant nitrogen fractions in which average soil mass recovery and average soil nitrogen recovery were both above 97%, and average mineralization contribution rate (soil labile and recalcitrant nitrogen fractions mineralization amount to that of original soil) was 99.91% (99.89%-99.93%). Net-nitrogen (Net-N) mineralization potential of soil labile nitrogen under treatments of NPK, NPKS and NPKM were higher than that under CK treatments by 26.82%-137.10% (except NPK of black soil). Different fertilizer treatments had a significant influence on Net-N mineralization potential of soil labile nitrogen in black soil and fluvo-aquic soil. The Net-N mineralization potential of soil labile nitrogen in black soil under NPKM treatment was 1.48 mg?kg^-1?d^-1, which was higher compared with NPKS (1.02 mg?kg^-1?d^-1) and NPK (0.75 mg?kg^-1?d^-1). The Net-N mineralization potential of soil labile nitrogen in fluvo-aquic soil under NPKM treatment was 1.17 mg?kg^-1?d^-1, which was higher compared with NPKS (0.89 mg?kg^-1?d^-1) and NPK (0.76 mg?kg^-1?d^-1). The Net-N mineralization potential of soil recalcitrant nitrogen had no significant difference among different fertilizer treatments. The average mineralization potential was 0.58 mg?kg^-1?d^-1(0.52-0.63 mg?kg^-1?d^-1) in black soil and 0.51 mg?kg^-1?d^-1(0.40-0.62 mg?kg^-1?d^-1) in fluvo-aquic soil. The Net-N mineralization potential of soil labile nitrogen was higher than that of soil recalcitrant nitrogen in both black soil and fluvo-aquic soil, and it showed the biggest difference under the NPKM treatment. Among them, the Net-N mineralization potential of soil labile nitrogen was 1.41, 1.39, 1.75, and 2.35 times that of soil recalcitrant nitrogen as the order of CK, NPK, NPKS, and NPKM in black soil, and was 1.22, 1.33, 1.56, and 1.87 times as the same order in fluvo-aquic soil. The mineralization contribution rate of soil labile nitrogen was influenced by different fertilizer measures in this order CK, NPKsignificantly higher than the other treatments. There were no significant differences among the mineralization contribution rate of upland recalcitrant nitrogen under different fertilizer treatments. The average mineralization contribution rates were 86.24% (83.96%-88.51%) in black soil and 88.46% (86.71%-90.20%) in fluvo-aquic soil. 【Conclusion】 The Net-N mineralization potential and mineralization contribution rates of upland soil labile nitrogen were more sensitive to different fertilizer measures than that in upland soil recalcitrant nitrogen. Under different fertilization measures, the Net-N mineralization potential of soil labile nitrogen was significantly higher than that of soil recalcitrant nitrogen, especially for the treatment of NPKM. Long-term application of NPKS or NPKM will improve Net-N mineralization potential and mineralization contribution rate of upland soil. The improvement effect is in the order of NPKM＞NPKS＞NPK.

Key words: liable nitrogen, recalcitrant nitrogen, black soil, fluvo-aquic soil, characteristics of mineralization; long-term fertilization system

武红亮，于维水，朱平，张水清，赵雅雯，刘婧，王士超，孟繁华，卢昌艾. 长期施肥旱地土壤易分解与耐分解氮的矿化特性[J]. 中国农业科学, 2015, 48(23): 4698-4706.

WU Hong-liang, YU Wei-shui, ZHU Ping, ZHANG Shui-qing, ZHAO Ya-wen, LIU Jing, WANG Shi-chao, MENG Fan-hua, LU Chang-ai. Mineralization Characteristics of Upland Soil Labile and Recalcitrant Nitrogen Under Long-Term Different Fertilization Systems[J]. Scientia Agricultura Sinica, 2015, 48(23): 4698-4706.

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参考文献

[1] 胡田田, 李生秀, 郝乾坤. 旱地土壤矿质氮和可矿化氮与土壤供氮能力的关系. 水土保持学报, 2000, 14(4): 83-86, 103.

Hu T T, Li S X, Hao Q K. Relationship between soil mineralized N, mineralizable N and soil nitrogen-supplying capacity. Journal of Soil and Water Conservation, 2000, 14(4): 83-86,103. (in Chinese)

[2] 李生秀. 中国旱地土壤植物氮素. 北京: 科学出版社, 2008.

Li S X. Plants Nitrogen of Upland Soil in China. Beijing: Science Press, 2008. (in Chinese)

[3] 王媛, 周建斌, 杨学云. 长期不同培肥处理对土壤有机氮组分及氮素矿化特性的影响. 中国农业科学, 2010, 43(6): 1173-1180.

Wang Y, Zhou J B, Yang X Y. Effects of different long-term fertilization on the fractions of organic nitrogen and nitrogen mineralization in soils. Scientia Agricultura Sinica, 2010, 43(6): 1173-1180. (in Chinese)

[4] 李世清, 沈玉芳. 黄土高原土壤有机氮及其矿化. 北京: 科学出版社, 2010.

Li S Q, Shen Y F. Soil Organic Nitrogen and Mineralization of the Loess Plateau in China. Beijing: Science Press, 2010. (in Chinese)

[5] 田茂洁. 土壤氮素矿化影响因子研究进展. 西华师范大学学报: 自然科学版, 2004, 25(3): 298-303.

Tian M J. Review on the contributing factors to mineralization of soil nitrogen. Journal of China West Normal University: Natural Sciences, 2004, 25(3): 298-303. (in Chinese)

[6] Stanford G. Effect of partial removal of soil organic N with sodium pyrophosphate in sulfuric acid solution on subsequent mineralization of nitrogen. Soil Science Society of America Journal, 1968, 32(5): 679-682.

[7] 金发会, 李世清, 卢红玲, 李生秀. 石灰性土壤供氮能力几种生物测定方法的评价研究. 中国农业科学, 2007, 40(7): 1422-1431.

Jin F H, Li S Q, Lu H L, Li S X. Estimation of the biological methods on assessing soil nitrogen-supplying capacity in calcareous soil. Scientia Agricultura Sinica, 2007, 40(7): 1422-1431. (in Chinese)

[8] 李菊梅, 王朝辉, 李生秀. 有机质、全氮和可矿化氮在反映土壤供氮能力方面的意义. 土壤学报, 2003, 40(2): 232-238.

Li J M, Wang Z H, Li S X. Significance of soil organic matter, total N and mineralizable nitrogen in reflecting soil N supplying capacity. Acta Pedologica Sinica, 2003, 40(2): 232-238. (in Chinese)

[9] 刘晓宏, 郝明德, 田梅霞. 土壤矿质氮和可矿化氮对当季作物的贡献. 土壤与环境, 2001, 10(3): 207-209.

Liu X H, Hao M D, Tian M X. Contribution of soil mineral nitrogen and soil mineralization nitrogen to seasonal crop. Soil and Environmental Sciences, 2001, 10(3): 207-209. (in Chinese)

[10] Nuske A, Richter J. N-mineralization in loss-parabrownearthes: incubation experiments. Plant and Soil, 1981, 59: 237-247.

[11] Standford G, Smith S J. Nitrogen mineralization potentials of soils. Soil Science Society of America Journal, 1972, 36(3): 465-472.

[12] Meijboom F W, Hassink J, Van Noordwijk M. Density fractionation of soil macroorganic matter using silica suspensions. Soil Biology and Biochemistry, 1995, 27(8): 1109-1111.

[13] Huygens D, Rütting T, Boeckx P, Van Cleemput O, Godoy R, Müller C. Soil nitrogen conservation mechanisms in a pristine south Chilean Nothofagus forest ecosystem. Soil Biology and Biochemistry, 2007, 39(10): 2448-2458.

[14] Müller C, Stevens R J, Laughlin R J. A ¹⁵N tracing model to analyse N transformations in old grassland soil. Soil Biology and Biochemistry, 2004, 36(4): 619-632.

[15] Deans J R, Molina A E, Clapp C E. Models for predicting potentially mineralizable nitrogen and decomposition rate constants. Soil Science Society of America Journal, 1986, 50(2): 323-326.

[16] El- Gharous M, Westerman R L, Soitanpous P N. Nitrogen mineralizatin potential of arid and semiarid soils of Morocco. Soil Science Society of America Journal, 1990, 54(2): 438-443.

[17] Curtin D, Wen G. Organic matter fractions contributing to soil nitrogen mineralization potential. Soil Science Society of America Journal, 1999, 63(2): 410-415.

[18] 徐明岗, 梁国庆, 张夫道. 中国土壤肥力演变. 北京: 中国农业科学技术出版社, 2006.

Xu M G, Liang G Q, Zhang F D. Soil Fertility Evolution in China. Beijing: China Agricultural Science and Technology Press, 2006. (in Chinese)

[19] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科学技术出版社, 2000.

Lu R K. Analysis Method of Soil and Agro-Chemistry. Beijing: China Agricultural Science and Technology Press, 2000. (in Chinese)

[20] Steinweg J M, Fisk M C, McAlexander B, Groffman P M, Hardy J P. Experimental snowpack reduction alters organic matter and net N mineralization potential of soil macroaggregates in a northern hardwood forest. Biology and Fertility of Soils, 2008, 45(1): 1-10.

[21] 巨晓棠, 边秀举, 刘学军, 张福锁, 毛达如. 干旱土壤氮素矿化参数与氮素形态的关系. 植物营养与肥料学报, 2000, 6(3): 251-259.

Ju X T, Bian X J, Liu X J, Zhang F S, Mao D R. Relationship between soil nitrogen mineralization parameter with several nitrogen forms. Plant Nutrition and Fertilizer Science, 2000, 6(3): 251-259. (in Chinese)

[22] Fox T R. Nitrogen mineralization following fertilization of douglas-fir forests with urea in western Washington. Soil Science Society of America Journal, 2004, 68(5): 1720-1728.

[23] 鲁彩艳, 牛明芬, 陈欣, 史奕, 石险峰. 不同施肥制度培育土壤氮矿化势与供氮潜力. 辽宁工程技术大学学报, 2007, 26(5): 773-775.

Lu C Y, Niu M F, Chen X, Shi Y, Shi X F. Nitrogen mineralization potentials of meadow brown soil in different fertilization practice. Journal of Liaoning Technical University, 2007, 26(5): 773-775. (in Chinese)

[24] 李世清, 李生秀. 有机物料在维持土壤微生物体氮库中的作用. 生态学报, 2001, 21(1): 136-142.

Li S Q, Li S X. Effects of organic materials on maintaining soil microbial biomass nitrogen. Acta Ecologica Sinica, 2001, 21(1): 136-142. (in Chinese)

[25] 杨巧丽, 姚拓, 王得武, 滚双宝. 木质纤维分解菌群筛选及其对秸秆分解与畜禽粪便除臭能力评价. 草业学报, 2015, 24(1): 196-203.

Yang Q L, Yao T, Wang D W, Gun S B. Screening of lignocelluloses degrading microbial communities for their ability to deodorize livestock and poultry wastes. Acta Prataculturae Sinica, 2015, 24(1): 196-203. (in Chinese)

[26] 李贵桐, 赵紫娟, 黄元仿, 李保国. 秸秆还田对土壤氮素转化的影响. 植物营养与肥料学报, 2002, 8(2): 162-167.

Li G T, Zhao Z J, Huang Y F, Li B G. Effect of straw returning on soil nitrogen transformation. Plant Nutrition and Fertilizer Science, 2002, 8(2): 162-167. (in Chinese)

[27] 张玉玲, 张玉龙, 虞娜, 姬景红. 长期不同施肥对水稻土有机氮素矿化特性影响的研究. 植物营养与肥料学报, 2008, 14(2): 272-276.

Zhang Y L, Zhang Y L, Yu N, Ji J H. Effect of different long-term fertilizing practices on nitrogen mineralization characteristic of paddy soil. Plant Nutrition and Fertilizer Science, 2008, 14(2): 272-276. (in Chinese)

[28] Klemmedson J O, Rehfuess K E, Makeschin F, Rodenkirchen H. Nitrogen mineralization in lime- and gypsum-amended substrates from ameliorated acid forest soils. Soil Science, 1989, 147(1): 55-63.

[29] Whalen J K, Bottomley P J, Myrold D D. Carbon and nitrogen mineralization from light- and heavey- fraction additions to soil. Soil Biology and Biochemistry, 2000, 32(10): 1345-1352.

[30] Puget P, Chenu C, Balesdent J. Dynamics of soil organic matter associated with particle-size fractions of water-stable aggregates. European Journal of Soil Science, 2000, 51: 595-605.

[31] Yamashita T, Flessa H, John B, Helfrich M, Ludwig B. Organic matter in density fractions of water-stable aggregates in silty soils: Effect of land use. Soil Biology and Biochemistry, 2006, 38(11): 3222-3234.

[32] Tan Z, Lal R, Owens L, Izaurralde R C. Distribution of light and heavy soil organic carbon as related to land use and tillage practice. Soil and Tillage Research, 2007, 92: 53-59.

[33] Six J, Paustain K, Eillott E T, Combrink C. Soil structure and organic matter: I. Distribution of aggregate-size classes and aggregate- associated carbon. Soil Science Society of America Journal, 2000, 64(2): 681-689.

长期施肥旱地土壤易分解与耐分解氮的矿化特性

Mineralization Characteristics of Upland Soil Labile and Recalcitrant Nitrogen Under Long-Term Different Fertilization Systems

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[10]	马原,迟美静,张玉玲,范庆峰,虞娜,邹洪涛. 黑土旱地改稻田土壤水稳性团聚体有机碳和全氮的变化特征[J]. 中国农业科学, 2020, 53(8): 1594-1605.
[11]	刘凯,刘佳,陈晓芬,李委涛,江春玉,吴萌,樊剑波,李忠佩,刘明. 长期施用磷肥水稻土微生物量磷的季节变化特征与差异[J]. 中国农业科学, 2020, 53(7): 1411-1418.
[12]	魏丹,蔡姗姗,李艳,金梁,王伟,李玉梅,白杨,胡钰. 黑土水溶性有机碳对有机物料还田的响应[J]. 中国农业科学, 2020, 53(6): 1180-1188.
[13]	李小磊,张玉军,申凤敏,姜桂英,刘芳,柳开楼,刘世亮. 长期施肥对红壤性水稻土不同土层活性有机质及碳库管理指数的影响[J]. 中国农业科学, 2020, 53(6): 1189-1201.
[14]	张秀芝,李强,高洪军,彭畅,朱平,高强. 长期施肥对黑土水稳性团聚体稳定性及有机碳分布的影响[J]. 中国农业科学, 2020, 53(6): 1214-1223.
[15]	李亚林,张旭博,任凤玲,孙楠,徐梦,徐明岗. 长期施肥对中国农田土壤溶解性有机碳氮含量影响的整合分析[J]. 中国农业科学, 2020, 53(6): 1224-1233.