降铜对植酸酶在断奶仔猪饲粮中应用的影响

doi:10.3864/j.issn.0578-1752.2015.14.020

摘要/Abstract

摘要： 【目的】为加快仔猪生长和提高饲粮消化率，仔猪饲粮中常常同时添加高剂量铜和植酸酶，但高铜对植酸酶的作用究竟有何影响却仍鲜见报道。研究高铜饲粮中添加植酸酶对仔猪营养物质消化率、生产性能、粪铜和粪磷排放的影响，以及降低铜的添加量对植酸酶作用的影响。【方法】试验选取180头体重9—10 kg长×大二元杂交仔猪，随机分成3组，每组5栏，每栏12头猪，分别饲喂3种试验饲粮中的一种。试验饲粮为玉米-豆粕型饲粮，其营养水平达到或超过NRC(1998)相应阶段猪营养需要量。组1为对照组，饲粮铜添加量为180 mg·kg^-1（硫酸铜），不添加植酸酶；组2和组3为植酸酶试验组，添加500 IU·kg^-1植酸酶，组2和3饲粮铜添加量分别为 180和120 mg·kg^-1（硫酸铜）。试验预试期3 d，正试期3 w，试验期间仔猪自由采食和饮水，记录采食量，观察腹泻情况，计算试验期间平均日采食量、日增重、料重比和腹泻频率。试验结束前连续3 d以栏为重复单位采集部分新鲜粪样，每100 g新鲜粪样加10 mL的10%乙醇，-20℃保存待测。以酸不溶灰分为参照内标，测定计算营养物质消化率和矿物质表观消化率。【结果】（1）高铜仔猪饲粮中添加植酸酶可使磷表观消化率提高21.12%（组2与组1相比，P＜0.01），粪磷含量降低4.41%（P=0.09），腹泻频率降低19.22%（P=0.35），添加植酸酶对干物质和蛋白表观消化率，采食量、日增重和料重比均无显著影响。（2）两个植酸酶试验组相比，当铜添加量由180 mg·kg^-1降低到120 mg·kg^-1时，钙、磷表观消化率分别提高13.74%和9.79%（组3与组2相比，P=0.02和P=0.01），粪磷含量降低18.86%（P＜0.01），腹泻频率降低25.24%（P=0.25），降铜对干物质和粗蛋白表观消化率、采食量、日增重和料重比均无显著影响。（3）与高铜无植酸酶组相比，减少铜添加量并且添加植酸酶，可使饲粮的干物质、粗蛋白、钙和磷表观消化率分别提高1.80%、2.84%、17.20%和32.98%（组3与组1相比，P=0.02，P=0.04，P＜0.01和P＜0.01），腹泻频率降低39.6%（P=0.04）。【结论】现有试验表明，在添加植酸酶的玉米-豆粕型饲粮中，将铜（硫酸铜形式）添加量从180 mg·kg^-1 减少到120 mg·kg^-1可以显著减少铜对植酸酶的干扰，提高饲粮钙、磷表观消化率，降低粪铜和粪磷含量。

关键词: 植酸酶, 仔猪, 生产性能, 消化率, 粪铜

Abstract: 【Objective】To promote the growth of piglets and nutrient digestibility, high copper and/or phytase additives are usually added to weaned piglet diets. However, the effects of high dietary copper on the phytase application are not very clear. This study was conducted to determine the effects of supplementation of phytase on nutrient apparent digestibility (AD), growth performance, fecal copper (FCu) and fecal phosphorus (FP) in piglets with high copper diets, and the effects of reducing copper dosage on the phytase application. 【Method】Total of 180 Landrace ´ Yorkshire piglets with initial body weight of 9-10 kg were randomly assigned to three dietary treatments group, 5 replicate pens of 12 piglets in each group. The diets based on corn-soybean meal meet or exceed NRC (1998) nutrient requirements. For the first group (control group, Group 1), 180 mg·kg^-1 copper from copper sulfate addition was added to the diet without phytase. For the second and third group (phytase treatment groups, Group 2 and Group 3), 180 and 120 mg·kg^-1 copper from copper sulfate addition was added to the diets with 500 IU·kg^-1 phytase, respectively. Feed and water were provided ad libitum throughout the three weeks of experimental period, following by three days of adaptation. Feed consumption and diarrhea were checked daily, and body weight was recorded at the beginning and end of the experiment to calculate average daily bodyweight gain (ADG), average daily feed intake (ADFI), feed conversion ratio (F/G) and diarrhea frequency. At the last week, fececs were spot sampled for three consecutive days from each pen. Every 100 gram of feces was immediately added with 10 mL of 10% ethanol and stored at -20°C until required for further analysis. Nutrients apparent digestibilities were calculated using acid-insoluble ash as an index in feed and fecal samples. 【Result】 The results showed that adding phytase to high copper level diet increased phosphorus (P) apparent digestibility by 21.12% (Group 2 to Group 1, P＜0.05), decreased FCu by 4.41% (P=0.09) and diarrhea frequency by 19.22 % (P=0.35). No significant difference was observed in apparent digestibility of dry matter (DM) and crude protein (CP), ADFI, ADG or F/G. Compared with two phytase treatment groups, reducing the copper supplement from 180 mg·kg^-1to 120 mg·kg^-1 increased the apparent digestibility of calcium (Ca) and P by 13.74% and 9.79% (Group 3 to Group 2, P=0.02 and P=0.01), decreased FP by 18.86% (P＜0.01) and diarrhea frequency by 25.24% (P=0.25). No significant difference was observed in digestibility of DM, CP, ADFI, ADG or F/G. In contrast to high copper diet without phytase addition, lower copper diet with phytase addition showed higher apparent digestibility of DM, CP, Ca and P by 1.80%, 2.84%, 17.20% and 32.98% (Group 3 compare with Group 1, P=0.02, P=0.04，P＜0.01 and P＜0.01) and lower diarrhea frequency by 39.6% (P=0.04). 【Conclusion】Results of the current experiment indicate that reducing copper supplementation in the corn-soybean meal basal diet from 180 mg·kg^-1 to 120 mg?kg^-1 could prevent severely diminishing phytase’s positive effects on Ca and P apparent digestibility and FCu and FP content.

Key words: phytase, piglets, performance, digestibility, fecal copper

陆扬，胡二永，字正浩，孙国荣，夏东. 降铜对植酸酶在断奶仔猪饲粮中应用的影响[J]. 中国农业科学, 2015, 48(14): 2884-2890.

LU Yang, HU Er-yong, ZI Zheng-hao, SUN Guo-rong, XIA Dong . Improvement of the Effects of Phytase Application by Lowering the High Level of Copper in Piglets Diets[J]. Scientia Agricultura Sinica, 2015, 48(14): 2884-2890.

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参考文献

[1] Li S F, Niu Y B, Liu J S, Lu L, Zhang L Y, Ran C Y, Feng M S, Du B, Deng J L, Luo X G. Energy, amino acid, and phosphorus digestibility of phytase transgenic corn for growing pigs. Journal of Animal Science, 2013, 91(1):298-308.

[2] Roberson K D. Estimation of the phosphorus requirement of weanling pigs fed supplemental phytase. Animal Feed Science and Technology, 1999, 80(2):91-100.

[3] 张克英, 陈代文, 余冰, 罗献梅, 李永义. 饲粮中添加植酸酶对断奶仔猪生长性能及蛋白质、氨基酸和磷利用率的影响. 动物营养学报, 2001, 13(3):19-24.

Zhang K Y, Chen D W, Yu B, Luo X M, Li Y Y. The performance and protein, amino acid and phosphorus utilization of piglets fed diets added with phytase. Acta Zoonutrimenta Sinica, 2001, 13(3):19-24. (in Chinese)

[4] Selle P H, Ravindran V. Phytate-degrading enzymes in pig nutrition. Livestock Science, 2008, 113(2/3):99-122.

[5] Bikker P, Jongbloed A W, Thissen J T. Meta-analysis of effects of microbial phytase on digestibility and bioavailability of copper and zinc in growing pigs. Journal of Animal Science, 2012, 90(Suppl 4):134-136.

[6] Jolliff J S, Mahan D C. Effect of dietary inulin and phytase on mineral digestibility and tissue retention in weanling and growing swine. Journal of Animal Science, 2012, 90(9):3012-3022.

[7] NRC. Nutrient Requirements of Swine. Washington D C: The National Academies Press, 2012.

[8] Barber R S, Braude R, Mitchell K G. Antibiotic and copper supplements for fattening pigs. British Journal of Nutrition, 1955, 9(4):378-381.

[9] Maenz D D, Engele-Schann C M, Newkird R W. The effect of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible forms of phytic acid in solation and in a slurry of canola meal. Animal Feed Science Technology, 1999(3/4):177-192.

[10] Lu L, Hao S, Zhang L, Luo X. Effect of copper source on phytase stability in the premixes of weanling piglets. Animal Production Science, 2013, 53:142-145.

[11] Lu L, Wang R L, Zhang Z J, Steward F A, Luo X, Liu B. Effect of dietary supplementation with copper sulfate or tribasic copper chloride on the growth performance, liver copper concentrations of broilers fed in floor pens, and stabilities of vitamin E and phytase in feeds. Biological Trace Element Research, 2010, 138(1/3):181-189..

[12] NRC. Nutrient Requirements of Swine. Washington D C: The National Academies Press, 1998.

[13] 王九峰, 李同洲, 译. 动物营养. 6 ed. 北京: 中国农业大学出版社, 2007.

Wang J F, Li T Z translated. Animal Nutrition. 6 ed. Beijing: China Agricultural University Press, 2007. (in Chinese)

[14] 张丽英. 饲料分析及饲料质量检测技术. 北京: 中国农业大学出版社, 2002.

Zhang L Y. Feed Analysis and Feed Quality Inspection Technology. Beijing: China Agricultural University Press, 2002. (in Chinese)

[15] Emiola A, Akinremi O, Slominski B, Nyachoti C M. Nutrient utilization and manure P excretion in growing pigs fed corn-barley- soybean based diets supplemented with microbial phytase. Journal of Animal Science, 2009, 80(1):19-26.

[16] Varley P F, Callan J J, O’Doherty J V. Effect of dietary phosphorus and calcium level and phytase addition on performance, bone parameters, apparent nutrient digestibility, mineral and nitrogen utilization of weaner pigs and the subsequent effect on finisher pig bone parameters. Animal Feed Science and Technology, 2011, 165(3/4): 201-209.

[17] Yanez J L, Landero J L, Owusu-Asiedu A, Cervantes M, Zijlstra R T. Growth performance, diet nutrient digestibility, and bone mineralization in weaned pigs fed pelleted diets containing thermostable phytase. Journal of Animal Science, 2013, 91(2): 745-754.

[18] Selle P H, Cowieson A J, Cowieson N P, Ravindran V. Protein-phytate interactions in pig and poultry nutrition: a reappraisal. Nutrition Research Reviews, 2012, 25(1):1-17.

[19] Shockravi S, Mohammad-Shirazi M, Abadi A, Seyedain M, Kimiagar M. Phytase supplementation improves blood zinc in rats fed with high phytate Iranian bread. Journal of Research in Medical Sciences, 2012, 17(4):361-367.

[20] McClung J P, Stahl C H, Marchitelli L J, Morales-Martinez N, Mackin K M, Young A J, Scrimgeour A G. Effects of dietary phytase on body weight gain, body composition and bone strength in growing rats fed a low-zinc diet. The Journal of Nutritional Biochemistry, 2006, 17(3): 190-196.

[21] Scrimgeour A G, Marchitelli L J, Whicker J S, Song Y, Ho E, Young A J. Phytase supplementation increases bone mineral density, lean body mass and voluntary physical activity in rats fed a low-zinc diet. Journal of Nutritional Biochemistry, 2010, 21(7):653-658.

[22] Akhter S, Saeed A, Irfan M, Malik K A. In vitro dephytinization and bioavailability of essential minerals in several wheat varieties. Journal of Cereal Science, 2012, 56(3):741-746.

[23] Pallauf J, Höhler D, Rimbach G. Effect of microbial phytase supplementation to a maize–soya-diet on the apparent absorption of Mg, Fe, Cu, Mn and Zn and parameters of Zn-status in piglets. Journal of Animal Physiology and Animal Nutrition (Berl), 1992, 68:1-9.

[24] Madrid J, Martínez S, López C, Hernández F. Effect of phytase on nutrient digestibility, mineral utilization and performance in growing pigs. Livestock Science, 2013, 154(1/3):144-151.

[25] Woyengo T A, Adeola O, Udenigwe C C, Nyachoti C M. Gastro-intestinal digesta pH, pepsin activity and soluble mineral concentration responses to supplemental phytic acid and phytase in piglets. Livestock Science, 2010, 134(1/3):91-93.

[26] 计成, 蔡青和, 岳洪源. 添加植酸酶对仔猪生长和营养物质回肠表观消化率的影响. 中国农业大学学报, 2003, 8(1):87-90.

Ji C, Cai Q H, Yue H Y. Efect of phytase supplementation on growth performance and apparent ileal digestibility in weaned pigs. Journal of Clum Agricultural University, 2003,8(1):87-90. (in Chinese)

[27] Kuhn I, Partanen K. Phytase improves apparent total tract digestibility of phosphorus and calcium in piglets fed diets with adequate or reduced phosphorus content. Journal of Animal Science, 2012, 90 (Suppl 4):194-196.

[28] Poulsen H D, Carlson D, Nørgaard J V, Blaabjerg K. Phosphorus digestibility is highly in?uenced by phytase but slightly by calcium in growing pigs. Livestock Science, 2010, 134(1):100-102.

[29] Pang Y, Applegate T J. Effects of copper source and concentration on in vitro phytate phosphorus hydrolysis by phytase. Journal of Agricultural and Food Chemistry, 2006, 54(5):1792-1796.

[30] Adeola O, Lawrence B V, Sutton A L, Cline T R. Phytase-induced changes in mineral utilization in zinc-supplemented diets for pigs. Journal of Animal Science,1995, 73(11):3384-3391.

[31] Walk C L, Srinongkote S, Wilcock P. Influence of a microbial phytase and zinc oxide on young pig growth performance and serum minerals. Journal of Animal Science, 2013, 91(1):286-291.

[32] Champagne E T, Fisher M S, Hinojosa O. NMR and ESR studies of interactions among divalent cations, phytic acid, and N-acetyl-amino acids. Journal of Inorganic Biochemistry, 1990, 38(3):199-215.

[33] 刘晓波, 罗绪刚. 高剂量铜对猪促生长作用机理的研究进展. 动物营养学报, 1997, 9(3):1-6.

Liu X B, Luo X G. Recent advances in the mode of action of copper for the growth stimulation of pigs: a review. Chinese Journal of Animal

Nutrition, 1997, 9(3):1-6. (in Chinese)

[34] 郭彤, 许梓荣. 铜离子对引起仔猪腹泻的大肠杆菌K88杀菌机理的研究. 中国预防兽医学报, 2004, 26(3):127-130.

Guo T, Xu Z Y. Studies on antibacterial mechanism of cupric ions in Escherichia coli K88. Chinese Journal of Preventive Veterinary Medicine, 2004, 26(3):127-130. (in Chinese)

[35] Ball R O, Aherne F X. Influence of dietary nutrient density, level of feed intake and weaning age on young pigs. II. Apparent nutrient digestibility and incidence and severity of diarrhea. Canadian Journal of Animal Science, 1987, 67(4):1105-1115.