花后轻干-湿交替灌溉提高水稻籽粒淀粉合成相关基因的表达

doi:10.3864/j.issn.0578-1752.2015.07.04

中国农业科学 ›› 2015, Vol. 48 ›› Issue (7): 1288-1299.doi: 10.3864/j.issn.0578-1752.2015.07.04

花后轻干-湿交替灌溉提高水稻籽粒淀粉合成相关基因的表达

陈婷婷^1,2，许更文¹，钱希旸¹，王志琴¹，张耗¹，杨建昌¹

¹扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心，江苏扬州225009
²中国水稻研究所/水稻生物学国家重点实验室，杭州 310006

收稿日期:2014-04-25 出版日期:2015-04-01 发布日期:2015-04-01
通讯作者: 杨建昌，Tel：0514-87979317；E-mail：jcyang@yzu.edu.cn
作者简介:陈婷婷，Tel：0571-63370370；E-mail：ntchtt@163.com
基金资助:
国家自然科学基金（31461143015，31271641，31071360，31471438）、国家“863”计划项目（2014AA10A605）、国家公益性行业（农业）科研专项（201103003，201203079，201203029）、国家“十二五”科技支撑计划项目（2011BAD16B14，2012BAD04B08，2013BAD07B09）、江苏省农业三新工程项目（SXG2014313）、江苏高校优势学科建设工程专项、中央级公益性科研院所基本科研业务费专项项目（2014RG004-4）

Post-Anthesis Alternate Wetting and Moderate Soil Drying Irrigation Enhance Gene Expressions of Enzymes Involved in Starch Synthesis in Rice Grains

CHEN Ting-ting^1,2, XU Geng-wen¹, QIAN Xi-yang¹, WANG Zhi-qin¹, ZHANG Hao¹, YANG Jian-chang¹

¹Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University /Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou 225009, Jiangsu
²China National Rice Research Institute/State Key Laboratory of Rice Biology, Hangzhou 310006

Received:2014-04-25 Online:2015-04-01 Published:2015-04-01

摘要/Abstract

摘要： 【目的】水稻籽粒灌浆是光合同化产物向籽粒运输并合成淀粉的生理过程，决定水稻结实率、粒重高低及品质优劣。籽粒灌浆过程不仅受遗传因素的影响，而且受到温度和水分等环境因子的调节。灌溉是水稻生产上一项重要的技术，对水稻产量的形成有重要调控作用。但有关花后灌溉方式对水稻籽粒淀粉合成相关基因表达的影响，缺乏研究。本研究旨在探讨花后干湿交替灌溉对水稻籽粒灌浆的影响并阐明其分子机理。【方法】2012—2013年以两优培九（两系杂交籼稻）和扬粳4038（杂交粳稻）为材料种植于土培池，自抽穗（50%穗伸出剑叶叶鞘）至成熟设置3种灌溉方式处理：（1）常规灌溉（conventional irrigation，CI），保持浅水层，收获前一周断水；（2）轻干-湿交替灌溉（alternate wetting and moderate soil drying，WMD），自浅水层自然落干至土壤水势达-20 kPa时，灌水1—2 cm，再自然落干至土壤水势为-20 kPa，再上浅层水，如此循环；（3）重干-湿交替灌溉（alternate wetting and severe soil drying，WSD），自浅水层自然落干至土壤水势达-40 kPa时，灌水1—2 cm，再自然落干至土壤水势为-40 kPa，再上浅层水，如此循环。观察花后干湿交替灌溉对水稻强、弱势粒灌浆速率、粒重和淀粉合成相关酶活性的影响，并应用实时荧光定量PCR技术测定编码这些酶基因的相对表达量。【结果】强势粒的平均灌浆速率、粒重、淀粉合成有关的蔗糖合酶（SuS）、腺苷二磷酸葡萄糖焦磷酸化酶（AGP）、淀粉合酶（StS）和淀粉分支酶（SBE）等相关酶活性以及蔗糖合酶基因SuS2、SuS4，腺苷二磷酸葡萄糖焦磷酸化酶基因AGPL1、AGPL2、AGPL3、AGPS2，淀粉合酶基因SSI、SSIIa、SSIIc、SSIIIa和淀粉分支酶基因SBEI、SBEIIb的相对表达量在3种灌溉方式处理间没有显著差异。与常规灌溉相比，轻干-湿交替灌溉处理显著增加了弱势粒的平均灌浆速率、粒重、4种籽粒淀粉合成相关酶活性和除AGPL1外各基因的相对表达量，重干-湿交替灌溉处理则显著降低弱势粒的平均灌浆速率、粒重、各淀粉合成相关酶活性和基因的相对表达量。两供试品种试验结果趋势一致。相关分析表明，弱势粒的平均灌浆速率、粒重与SuS、AGP、StS、SBE活性以及SuS2、SuS4、AGPL2、AGPL3、AGPS2、SSI、SSIIa、SSIIc、SSIIIa、SBEI、SBEIIb基因的相对表达量呈极显著正相关关系。【结论】在轻干-湿交替灌溉处理下，水稻弱势粒淀粉合成相关酶活性及其基因表达的增强促进了弱势粒中淀粉的合成与积累，提高灌浆速率和增加粒重；而在重干-湿交替灌溉处理下，弱势粒中上述淀粉合成相关酶活性和基因表达的下降使其灌浆速率和粒重显著降低。

关键词: 水稻, 干湿交替灌溉, 强/弱势粒, 籽粒灌浆, 淀粉合成相关酶, 基因表达

Abstract: 【Objective】Grain filling in rice is a physiological process of transportation and conversion of photosynthetic assimilates into starch which determines the percentage of filled grains, grain weight, and rice quality. The process of grain filling could not only be affected by genetic factors, but also be regulated by environmental conditions such as temperature and moisture. Irrigation is an important technique in rice production and plays a vital role in regulating the formation of grain yield. However, little work has been done in the effect of postanthesis irrigation patterns on the expressions of genes encoding starch biosynthesis in rice grains. This study aims to investigate the effect of post-anthesis alternate wetting and drying irrigation on grain filling of rice and to understand its molecular mechanism.【Method】Two cultivars Liangyoupeijiu (two-line indica hybrids) and Yangjing 4038 (japonica hybrids) were used and grown in the cement tanks that were filled with soil. Three irrigation regimes were imposed from heading (50% of the panicles were protrudent the flag leaf sheath) to maturity: (1) conventional irrigation (CI), plots were kept a continuous shallow water layer until one week before harvest; (2) alternate wetting and moderate soil drying irrigation (WMD), the field was not irrigated until the soil water potential reached the threshold at -20 kPa, and then was flooded with 1-2 cm water depth, and was repeated this process again; (3) alternate wetting and severe soil drying irrigation (WSD), water in the plots was withheld until the soil water potential reached the threshold at -40 kPa, and then was flooded with 1-2 cm water depth, and this process was repeated again. The effect of irrigation regimes on grain filling rate, grain weight, activities of the enzymes involved in starch synthesis in superior and inferior spikelets, and expressions of the genes encoding these enzymes were determined using real-time quantitative PCR.【Result】The results showed that there were no significant differences in grain filling rate, grain weight, activities of enzymes involved in starch synthesis including sucrose synthase (SuS), adenosine diphosphate glucose pyrophosphorylase (AGP), starch synthase (StS), starch branching enzyme (SBE) and SuS2, SuS4, AGPL1, AGPL2, AGPL3, AGPS2, SSI, SSIIa, SSIIc, SSIIIa, SBEI and SBEIIb gene expressions in superior spikelets among the three irrigation treatments. Compared with CI, the WMD significantly increased the mean grain filling rate, grain weight, activities of the four enzymes involved in starch synthesis and their gene expressions in inferior spikelets except for AGPL1, whereas the WSD performed an opposite effect. The two trial cultivars behaved the same tendency. The correlation analysis showed that the mean grain filling rate and the grain weight of inferior spikelets were very significantly and positively correlated with activities of SuS, AGP, StS, SBE and gene expressions of SuS2, SuS4, AGPL2, AGPL3, AGPS2, SSI, SSIIa, SSIIc, SSIIIa, SBEI and SBEIIb. 【Conclusion】The results indicate that enhanced activities and gene expressions of the enzymes involved in starch synthesis in inferior spikelets under WMD contribute to their greater grain filling rate and grain weight through enhancing the starch synthesis and accumulation. The reduction in the grain filling rate and grain weight of inferior spikelets under WSD would be attributed to the decreased activities and gene expressions of the enzymes involved in starch synthesis.

Key words: rice, alternate wetting and drying irrigation, superior/inferior spikelets, grain filling, enzymes involved in starch synthesis, gene expression

陈婷婷，许更文，钱希旸，王志琴，张耗，杨建昌. 花后轻干-湿交替灌溉提高水稻籽粒淀粉合成相关基因的表达[J]. 中国农业科学, 2015, 48(7): 1288-1299.

CHEN Ting-ting, XU Geng-wen, QIAN Xi-yang, WANG Zhi-qin, ZHANG Hao, YANG Jian-chang. Post-Anthesis Alternate Wetting and Moderate Soil Drying Irrigation Enhance Gene Expressions of Enzymes Involved in Starch Synthesis in Rice Grains[J]. Scientia Agricultura Sinica, 2015, 48(7): 1288-1299.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2015.07.04

https://www.chinaagrisci.com/CN/Y2015/V48/I7/1288

参考文献

[1] Bouman B A M. A conceptual framework for the improvement of crop water productivity at different spatial scales. Agricultural Systems, 2007, 93: 43-60.

[2] Rejesus R M, Palis F G, Rodriguez D G P, Lampayan R M, Bouman B A M. Impact of the alternate wetting and drying (AWD) water-saving irrigation technique: Evidence from rice producers in the Philippines. Food Policy, 2011, 36(2): 280-288.

[3] Price A H, Norton G J, Salt D E, Ebenhoeh O, Meharg A A, Meharg C, Rafiqul Islam M, Sarma R N, Dasgupta Tapash, Ismail A M, McNally K L, Zhang H, Dodd I C, Davies W J. Alternate wetting and drying irrigation for rice in Bangladesh: Is it sustainable and has plant breeding something to offer? Food and Energy Security, 2013, 2(2): 120-129.

[4] Zhang H, Tan G, Yang L, Yang J, Zhang J, Zhao B. Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiology and Biochemistry, 2009, 47: 195-204.

[5] Tang T, Xie H, Wang Y, Lü B, Liang J. The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.). Journal of Experimental Botany, 2009, 60(9): 2641-2652.

[6] Yang J C, Zhang J H. Grain-?lling problem in ‘super’ rice. Journal of Experimental Botany, 2010, 61:1-5.

[7] Yang J, Peng S, Visperas R M, Sanico A L, Zhu Q, Gu S. Grain filling pattern and cytokinin content in the grains and roots of rice plants. Plant Growth Regulation, 2000, 30: 261-270.

[8] 吴世文, 高庆荣, 孙哲, 王茂婷, 孙正娟, 袁凯, 于松. 腺苷二磷酸葡萄糖焦磷酸化酶活性对小麦K、V、T型不育系育性及籽粒形成的影响. 作物学报, 2010, 36(6): 995-1002.

Wu S W, Gao Q R, Sun Z, Wang M T, Sun Z J, Yuan K, Yu S. Effects of ADP-glucose pyrophosphorylase activity on sterility and development of grain in K, V, and T-cytoplasmic male sterile lines in wheat. Acta Agronomica Sinica, 2010, 36(6): 995-1002. (in Chinese)

[9] Kang G Z, Xu W, Liu G Q, Peng X Q, Guo T C. Comprehensive analysis of the transcription of starch synthesis genes and the transcription factor RSR1 in wheat (Triticum aestivum) endosperm. Genome, 2013, 56(2): 115-122.

[10] Fu J, Xu Y, Chen L, Yuan L, Wang Z, Yang J. Changes in enzyme activities involved in starch synthesis and hormone concentrations in superior and inferior spikelets and their association with grain filling of super rice. Rice Science, 2013, 2: 120-128.

[11] Yan S, Li W, Yin Y, Wang Z. Sink strength in relation to growth of superior and inferior grains within a wheat spike. Journal of Agricultural Science, 2010, 148: 567-578.

[12] Jiang D, Cao W X, Dai T B, Jing Q. Activities of key enzymes for starch synthesis in relation to growth of superior and inferior grains on winter wheat (Triticum aestivum L.) spike. Plant Growth Regulation, 2003, 41: 247-257.

[13] Kato T, Shinmura D, Taniguchi A. Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types. Plant Production Science, 2007, 10: 442-450.

[14] Jeng T L, Wang C S, Chen C L, Sung J M. Effects of grain position on the panicle on starch biosynthetic enzyme activity in developing grains of rice cultivar Tainung 67 and its NaN₃-induced mutant. Journal of Agricultural Science, 2003, 141: 303-311.

[15] Duan M, Sun S S M. Profiling the expression of genes controlling rice grain quality. Plant Molecular Biology, 2005, 59: 165-178.

[16] Jeng T L, Wang C S, Tseng T H, Sung J M. Expression of granule-bound starch synthase in developing rice grain. Journal of the Science of Food and Agriculture, 2007, 87: 2456-2463.

[17] Ishimaru T, Hirose T, Matsuda T, Goto A, Takahashi K, Sasaki H, Terao T, Ishii R, Ohsugi R, Yamagishi T. Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle. Plant and Cell Physiology, 2005, 46: 620-628.

[18] Liang X Q, Chen Y X, Nie Z Y, Ye Y S, Liu J, Tian G M, Wang G H, Tuong T P. Mitigation of nutrient losses via surface runoff from rice cropping systems with alternate wetting and drying irrigation and site-specific nutrient management practices. Environmental Science and Pollution Research, 2013, 20(10): 6980-6991.

[19] Zhang H, Zhang S, Yang J, Zhang J, Wang Z.Postanthesis moderate wetting drying improves both quality and quantity of rice yield. Agronomy Journal, 2008, 100: 726-734.

[20] Zhang H, Chen T, Wang Z, Wang Z, Yang J, Zhang J. Involvement of cytokinins in the grain filling of rice under alternate wetting and drying irrigation. Journal of Experimental Botany, 2010, 61: 3719-3733.

[21] Richards F J. A flexible growth function for empirical use. Journal of Experimental Botany, 1959, 10: 290-300.

[22] 朱庆森, 曹显祖, 骆亦奇. 水稻籽粒灌浆的生长分析. 作物学报, 1988, 14(3): 182-193.

Zhu Q S, Cao X Z, Luo Y Q. Growth analysis on the progress of grain filling in rice. Acta Agronomica Sinica, 1988, 14(3): 182-193. (in Chinese)

[23] Zhu G, Ye N, Yang J, Peng X, Zhang J. Regulation of expression of starch synthesis genes by ethylene and ABA in relation to the development of rice inferior and superior spikelets. Journal of Experimental Botany, 2011, 62 (11): 3907-3916.

[24] Rajeevan M S, Ranamukhaarachi D G, Vernon S D, Unger E R. Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies. Methods, 2001, 25: 443-451.

[25] Chen J B, Wang S M, Jing R L, Mao X G. Cloning of PvP5CS gene from common bean (Phaseolus vulgaris) and its response to abiotic stresses. Journal of Plant Physiology, 2009, 166(1): 12-16.

[26] 蔡珊兰, 廖炳创, 潘丽朴, 朱国辉. 基因芯片分析水稻灌浆期籽粒淀粉合成相关基因的表达. 华南农业大学学报, 2013, 34(2): 187-191.

Cai S L, Liao B C, Pan L P, Zhu G H. Comprehensive expression analysis of starch biosynthesis related genes in the developing rice grains using DNA microarrays. Journal of South China Agricultural University, 2013, 34(2): 187-191. (in Chinese)

[27] Regina A, Kosar-Hashemi B, Ling S, Li Z, Rahman S, Morell M. Control of starch branching in barley defined through differential RNAi suppression of starch branching enzyme IIa and IIb. Journal of Experimental Botany, 2010, 61(5): 1469-1482.

[28] Cao Y N, Hu W G, Wang C S. Expression profiles of genes involved in starch synthesis in non-waxy and waxy wheat. Russian Journal of Plant Physiology-639., 2012, 59(5): 632

[29] Yang J C, Zhang J H, Wang Z Q, Xu G, Zhu Q. Activities of key enzymes in sucrose-to-starch conversion in wheat grains subjected to water deficit during grain filling. Plant Physiology, 2004, 135: 1621-1629.

[30] Hurkman W J, McCue K F, Altenbach S B, Korn A, Tanaka C K, Kothari K M, Johnson E L, Bechtel D B, Wilson J D, Anderson O D, DuPont F M. Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm. Plant Science, 2003, 164(5): 873-881.

[31] 汪结明, 张建, 江海洋, 朱苏文, 范军, 程备久. RNA干扰水稻SBE3基因的表达对籽粒淀粉合成及其关键酶活性的影响. 作物学报, 2010, 36(2): 313-320.

Wang J M, Zhang J, Jiang H Y, Zhu S W, Fan J, Cheng B J. Effects of RNA interference of SBE3 gene expression on starch accumulation and key enzymes activities involved in starch synthesis in transgenic rice grain. Acta Agromomica Sinica, 2010, 36(2): 313-320. (in Chinese)

[32] Peng Y B, Lu Y F, Zhang D P. Abscisic acid activates ATPase in developing apple fruit especially in fruit phloem cells. Plant, Cell and Environment, 2003, 26: 1329-1342.

[33] Yang J C, Zhang J H, Wang Z Q, Zhu Q S, Liu L J. Involvement of abscisic acid and cytokinins in the senescence and remobilization of carbon reserves in wheat subjected to water stress during grain filling. Plant, Cell and Environment, 2003, 26: 1621-1631.

[34] Chen X, Pierik R, Peeters A J, Poorter H, Visser E J, Huber H, de Kroon H, Voesenek L A. Endogenous abscisic acid as a key switch for natural variation in flooding-induced shoot elongation. Plant Physiology, 2010, 154, 969-977.

[35] Akihiro T, Mizuno K, Fujimura T. Gene expression of ADP-glucose pyrophosphorylase and starch contents in rice cultured cells are cooperatively regulated by sucrose and ABA. Plant and Cell Physiology, 2005, 46: 937-946.

[36] Zhang H, Li H, Yuan L, Wang Z, Yang J, Zhang J. Post-anthesis alternate wetting and moderate soil drying enhances activities of key enzymes in sucrose-to-starch conversion in inferior spikelets of rice. Journal of Experimental Botany, 2012, 63(1): 215-227.

花后轻干-湿交替灌溉提高水稻籽粒淀粉合成相关基因的表达

Post-Anthesis Alternate Wetting and Moderate Soil Drying Irrigation Enhance Gene Expressions of Enzymes Involved in Starch Synthesis in Rice Grains

RichHTML

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	肖德顺, 徐春梅, 王丹英, 章秀福, 陈松, 褚光, 刘元辉. 水培条件下根际氧环境对水稻幼苗磷吸收的影响及其生理机制[J]. 中国农业科学, 2023, 56(2): 236-248.
[2]	张晓丽, 陶伟, 高国庆, 陈雷, 郭辉, 张华, 唐茂艳, 梁天锋. 直播栽培对双季早稻生育期、抗倒伏能力及产量效益的影响[J]. 中国农业科学, 2023, 56(2): 249-263.
[3]	古丽旦,刘洋,李方向,成卫宁. 小麦吸浆虫小热激蛋白基因Hsp21.9的克隆及在滞育过程与温度胁迫下的表达特性[J]. 中国农业科学, 2023, 56(1): 79-89.
[4]	张克坤,陈可钦,李婉平,乔浩蓉,张俊霞,刘凤之,房玉林,王海波. 灌水量对限根栽培‘阳光玫瑰’葡萄果实发育与香气物质积累的影响[J]. 中国农业科学, 2023, 56(1): 129-143.
[5]	桑世飞,曹梦雨,王亚男,王君怡,孙晓涵,张文玲,姬生栋. 水稻氮高效相关基因的研究进展[J]. 中国农业科学, 2022, 55(8): 1479-1491.
[6]	韩晓彤,杨保军,李苏炫,廖福兵,刘淑华,唐健,姚青. 基于图像的水稻纹枯病智能测报方法[J]. 中国农业科学, 2022, 55(8): 1557-1567.
[7]	赵凌, 张勇, 魏晓东, 梁文化, 赵春芳, 周丽慧, 姚姝, 王才林, 张亚东. 利用高密度Bin图谱定位水稻抽穗期剑叶叶绿素含量QTL[J]. 中国农业科学, 2022, 55(5): 825-836.
[8]	蒋晶晶,周天阳,韦陈华,邬佳宁,张耗,刘立军,王志琴,顾骏飞,杨建昌. 不同栽培措施对超级稻强、弱势粒品质的影响[J]. 中国农业科学, 2022, 55(5): 874-889.
[9]	张亚玲, 高清, 赵羽涵, 刘瑞, 付忠举, 李雪, 孙宇佳, 靳学慧. 黑龙江省水稻种质稻瘟病抗性评价及抗瘟基因结构分析[J]. 中国农业科学, 2022, 55(4): 625-640.
[10]	束婧婷,单艳菊,姬改革,章明,屠云洁,刘一帆,巨晓军,盛中伟,唐燕飞,李华,邹剑敏. 广西麻鸡m6A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J]. 中国农业科学, 2022, 55(3): 589-601.
[11]	陈婷婷, 符卫蒙, 余景, 奉保华, 李光彦, 符冠富, 陶龙兴. 彩色稻叶片光合特征及其与抗氧化酶活性、花青素含量的关系[J]. 中国农业科学, 2022, 55(3): 467-478.
[12]	赫磊,路凯,赵春芳,姚姝,周丽慧,赵凌,陈涛,朱镇,赵庆勇,梁文化,王才林,朱丽,张亚东. 水稻穗顶端退化突变体paa21的表型分析及基因克隆[J]. 中国农业科学, 2022, 55(24): 4781-4792.
[13]	杜文婷,雷肖肖,卢慧宇,王云凤,徐佳星,罗彩霞,张树兰. 氮肥减量施用对我国三大粮食作物产量的影响[J]. 中国农业科学, 2022, 55(24): 4863-4878.
[14]	郭绍雷,许建兰,王晓俊,宿子文,张斌斌,马瑞娟,俞明亮. 桃XTH家族基因鉴定及其在桃果实贮藏过程中的表达特性[J]. 中国农业科学, 2022, 55(23): 4702-4716.
[15]	赵春芳,赵庆勇,吕远大,陈涛,姚姝,赵凌,周丽慧,梁文化,朱镇,王才林,张亚东. 半糯粳稻品种核心标记的筛选及DNA指纹图谱的构建[J]. 中国农业科学, 2022, 55(23): 4567-4582.