抽穗结实期不同时段高温对稻米品质的影响

doi:10.3864/j.issn.0578-1752.2013.14.003

摘要/Abstract

摘要： 【目的】研究抽穗结实期不同时段高温处理对稻米品质的影响，阐明高温对稻米品质的影响机理及其时段效应。【方法】选用耐热性不同的两个水稻品系，利用人工气候室进行抽穗结实期不同时段温度试验，研究抽穗结实期高温对稻米外观品质、加工品质、直链淀粉含量、胶稠度、蛋白质含量、RVA特征谱、Mg/ K及籽粒的淀粉粒结构的影响。【结果】抽穗结实期高温使两品系稻米长宽比变小，垩白粒率和垩白度增加，糙米率、精米率、整精米率降低，直链淀粉含量和蛋白质含量增加，胶稠度降低；淀粉RVA谱特征值崩解值降低，冷胶粘度、消减值和糊化温度升高，峰值时间延长，Mg、K含量增加，Mg/K比值降低；淀粉粒扫描电镜显示，高温条件下，淀粉粒间结合较为疏松，籽粒内大多以单个淀粉粒的形式存在，折光率下降，胚乳透明度降低，导致垩白形成。相同处理条件下，耐热品系996稻米品质受高温影响低于热敏感品系4628。抽穗结实期不同时段高温对稻米品质影响存在显著差异，以始穗后8—14 d对稻米品质影响最大，其次为始穗后15—21 d、1—7 d、始穗后22—28 d影响较小。【结论】始穗后8—21 d是温度对稻米品质影响的关键时期，对稻米品质形成具有决定作用，而结实后期（始穗22 d后）环境温度对稻米品质影响较弱。

关键词: 水稻 , 抽穗结实期 , 高温 , 稻米品质

Abstract: 【Objective】The effects of high temperature treatment at different times during heading and filling periods on rice quality were studied to ascertain the mechanism of the effect of high temperature on rice quality and its time effect. 【Method】The experiment was conducted with two rice lines, the heat-tolerant line 996 and heat-sensitive line 4628, in controlled environment chambers to investigate the effect of high temperature stress at different times during heading and filling stages on rice appearance quality, milling quality, amylose content, gel consistency, protein content, RVA characteristcs, Mg/K and starch granule structure of grain.【Result】The ratio of grain length to width, the brown rice rate, milled rice rate, head rice rate and gel consistency of both lines decreased under high temperature stress during heading and filling stages, while chalkness rate, chalkness, amylose content, protein content and gelatinization temperature increased. Under high temperature treatments, gelatinization temperature, final viscosity, set back and peak time increased, break dowm decreased, Mg content and K content increased, Mg/K decreased. The observation of starch structure with scanning electron microscope showed that the starch granules arranged loosely, most of the starch granules existed in the form of single starch granule, refractive index decreased, endosperm transparency decreased, and chalkiness was formed. Under the same treatment, the extent of rice quality of heat-tolerant line 996 affected by high temperature was lower than that of heat-sensitive line 4628. Rice qualities had different responses to different high temperature treatments, rice qualities were affected by high temperature treatment 8-14 d after heading mostly, moderatedly by high temperature treatment 15-21 d and 1-7 d after heading, while least by high temperature treatment 22-28 d after heading. 【Conclusion】Eight to twenty-one days after heading is the vital stage for whole rice quality in response to higher temperature, which affects rice qualities decisively; while the effect of temperature at the later stage(beyond 22 days from heading) is slight.

Key words: rice , heading and filling stage , high temperature , rice quality

张桂莲, 张顺堂, 王力, 肖应辉, 唐文帮, 陈光辉, 陈立云. 抽穗结实期不同时段高温对稻米品质的影响[J]. 中国农业科学, 2013, 46(14): 2869-2879.

ZHANG Gui-Lian, ZHANG Shun-Tang, WANG Li, XIAO Ying-Hui, TANG Wen-Bang, CHEN Guang-Hui, CHEN Li-Yun. Effects of High Temperature at Different Times During the Heading and Filling Periods on Rice Quality[J]. Scientia Agricultura Sinica, 2013, 46(14): 2869-2879.

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参考文献

[1]IPCC//Houghton J T, Ding Y, Griggs D J, Noguer M, van der Linden P J, Dai X, Maskell K, Johnson C A. Climate Change 2001: Scientific Basis. New York, USA: Cambridge University Press, 2001.

[2]王才林, 仲维功. 高温对水稻结实率的影响及其防御对策. 江苏农业科学, 2004(1): 15-18.

Wang C L, Zhong W G. Effects of high temperature on seed setting rate of rice and its prevention. Jiangsu Agricultural Science, 2004(1): 15-18. (in Chinese)

[3]赵海燕, 姚凤梅, 张勇, 徐宾, 袁静, 胡亚南, 许吟隆. 长江中下游水稻开花灌浆期气象要素与结实率和粒重的相关性分析. 中国农业科学, 2006, 39(9): 1765-1771.

Zhao H Y, Yao F M, Zhang Y, Xu B, Yuan J, Hu Y N, Xu Y L. Correlation analysis of rice seed setting rate and weight of 1000-grain and agro-meteorology over the middle and lower reaches of the Yangtze River. Scientia Agricultura Sinica, 2006, 39(9): 1765-1771. (in Chinese)

[4]金正勋, 杨静, 钱春荣, 刘海英, 金学泳, 秋太权. 灌浆成熟期温度对水稻籽粒淀粉合成关键酶活性及品质的影响. 中国水稻科学, 2005, 19(4): 377-380.

Jin Z X, Yang J, Qian C R, Liu H Y, Jin X Y, Qiu T Q. Effects of temperature during grain filling period on activities of key enzymes for starch synthesis and rice grain quality. Chinese Journal of Rice Science, 2005, 19(4): 377-380. (in Chinese )

[5]盛婧, 陶红娟, 陈留根. 灌浆结实期不同时段温度对水稻结实与稻米品质的影响. 中国水稻科学, 2007, 21(4): 396-402.

Sheng J, Tao H J, Chen L G. Response of seed-setting and grain quality of rice to temperature at different time during grain filling period. Chinese Journal of Rice Science, 2007, 21(4): 396-402. (in Chinese)

[6]Liang J S, Zhang J H, Cao X Z. Grain sink strength may be related to the poor grain filling of indica-japonica rice (Oryza sativa L.) hybrids. Physiological Plant, 2001, 112: 470-477.

[7]张国发, 王绍华, 尤娟, 王强盛, 丁艳锋, 吉志军. 结实期不同时段高温对稻米品质的影响. 作物学报, 2006, 32(2): 283-287.

Zhang G F, Wang S H, You J, Wang Q S, Ding Y F, Ji Z J. Effect of higher temperature in different filling stages on rice qualities. Acta Agronomica Sinica, 2006, 32(2): 283-287. (in Chinese)

[8]陶龙兴, 王熹, 廖西元, 沈波, 谈惠娟, 黄世文. 灌浆期气温与源库强度对稻米品质的影响及其生理分析. 应用生态学报, 2006, 17(4): 647-652.

Tao L X, Wang X, Liao X Y, Shen B, Tan H J, Huang S W. Physiological effects of air temperature and sink-source volume at milk-filling stage of rice on its grain quality. Chinese Journal of Appled Ecology, 2006, 17(4): 647-652. (in Chinese)

[9]董明辉, 陈培峰, 乔中英, 吴翔宙, 赵步洪, 蒋媛媛, 杨建昌. 水稻不同粒位籽粒米质对花后不同时段温度胁迫的响应. 作物学报, 2011, 37(3): 506-513.

Dong M H, Chen P F, Qiao Z Y, Wu X Z, Zhao B H, Jiang Y Y, Yang J C. Effect of temperature at different durations after anthesis on rice quality and variations between positions on a panicle. Acta Agronomica Sinica, 2011, 37(3): 506-513. (in Chinese )

[10]程方民, 胡东维, 丁元树. 人工控温条件下稻米垩白形成变化及胚乳扫描结构观察. 中国水稻科学, 2000, 14(2): 83-87.

Chen F M, Hu D W, Ding Y S. Dynamic change of chalkiness and observation of gain endosperm structure with scanning electron microscope under controlled temperature condition. Chinese Journal of Rice Science, 2000, 14(2): 83-87. (in Chinese)

[11]王丰, 程方民, 刘奕, 钟连进, 张国平. 不同温度下灌浆期水稻籽粒内源激素含量的动态变化. 作物学报, 2006, 32(1): 25-29.

Wang F, Cheng F M, Liu Y, Zhong L J, Zhang G P. Dynamic changes of plant hormones in developing grains at rice filling stage under different temperatures. Acta Agronomica Sinica, 2006, 32(1): 25-29. (in Chinese)

[12]Wang E T, Wang J J, Zhu X D, Hao W, Wang L Y, Li Q, Zhang X, He W, Lu B R, Lin H X, Ma H, Zhang G Q, He Z H. Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nature Genetics, 2008, 40: 1370-1374.

[13]符冠富, 王丹英, 李华, 陶龙兴, 章秀福. 水稻不同生育期温光条件对籽粒充实和米质的影响. 中国农业气象, 2009, 30(3): 375-382.

Fu G F, Wang D Y, Li H, Tao L X, Zhang X F. Influence of temperature and sunlight conditions on rice grain filling and quality in different growth stages. Chinese Journal of Agrometeorology, 2009, 30(3): 375-382. (in Chinese)

[14]董明辉, 赵步洪, 吴翔宙, 陈涛, 杨建昌. 水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系. 中国农业科学, 2008, 41(2): 370-380.

Dong M H, Zhao B H, Wu X Z, Chen T, Yang J C. Difference in hormonal content and activities of key enzymes in the grains at different positions on arice panicle during grain filling and their correlations with rice qualities. Scientia Agricultura Sinica, 2008, 41(2): 370-380. (in Chinese)

[15]韦克苏, 程方民, 董海涛, 张其芳, 刘奎刚, 曹珍珍. 水稻胚乳贮藏物代谢相关基因响应花后高温胁迫的微阵列分析. 中国农业科学, 2010, 43(1): 1-11.

Wei K S, Cheng F M, Dong H T, Zhang Q F, Liu K Q, Cao Z Z. microarray analysis of gene expression profile related to grain storage metabolism in rice endosperms as affected by high temperature at filling stage. Scientia Agricultura Sinica, 2010, 43(1): 1-11. (in Chinese)

[16]国家质量技术监督局. 中华人民共和国国家标准GB/T17891-1999: 优质稻谷. 北京: 中国标准出版社, 2000.

Supervising Department of Quality and Technology of China.The National Standard of the People’s Republic of China-GB/T17891— 1999: High Quality Paddy. Beijing: China Standard Press, 2000. (in Chinese)

[17]马启林, 李阳生, 田小海, 鄢圣之, 雷慰慈, 中田升. 高温胁迫对水稻贮藏蛋白质的组成和积累形态的影响. 中国农业科学, 2009, 42(2): 714-718.

Ma Q L, Li Y S, Tian X H, Yan S Z, Lei W C, Nakata N. Influence of high temperature stress on composition and accumulation configuration of storage protein in rice. Scientia Agricultura Sinica, 2009, 42(2): 714-718. (in Chinese)

[18]Krishnan P, Swain D K, Bhaskar B C, Nayak S K, Dash R N. Impact of elevated CO2 and temperature on rice yield and methods of adaptation as evaluated by crop simulation studies. Agriculture Ecosystems & Environment, 2007, 122: 233-242.

[19]Juliano B O, Bechtel D B. The rice grain and its gross composition// Juliano B O. Rice Chemistry and Technology. AM, ASSOC, Cereal Chemistry, USA, 1985, 62: 15-57.

[20]Lin C J, Li C Y, Lin S K, Yang F H, Huang J J, Liu Y H, Lur H S. Influence of high temperature during grain filling on the accumulation of storage proteins and grain quality in rice (Oryza sativa L.). Journal of Agricultural and Food Chemistry, 2010, 58(19): 10545-10552.

[21]钟旭华, 李太贵. 不同结实温度下稻米直链淀粉含量与千粒重的相关性研究. 中国水稻科学, 1994, 8(2): 126-128.

Zhong X H, Li T G. The correlation between amylose content and grain weight under different ripening temperature. Chinese Journal of Rice Science, 1994, 8(2): 126-128. (in Chinese) [22]贾志宽, 朱碧岩. 灌浆期气温的分布对稻米直链淀粉累积效应的初步研究. 云南农业大学学报, 1991, 6(2): 65-69.

Jia Z K, Zhu B Y. Preliminary study on the accumulation effect of amylose affected by the air temperature at the filling stage of rice. Journal of Yunnan Agricultural University, 1991, 6(2): 65-69. (in Chinese)

[23]莫惠栋. 我国稻米品质的改良. 中国农业科学, 1993, 26(4): 8-14.

Mo H D. Quality improvement of rice grain in China. Scientia Agricultura Sinica, 1993, 26(4): 8-14. (in Chinese)

[24]Yuji M, Koji O, Michikazu H. Differences in amylase content, mylographic characteristics and storage proteins of grain on primary and secondary rachis branches in rice. Japanese Journal of Crop Science, 1995, 64(3): 601-606.

[25]Lim S J. Varietal variation of amylogram properties and its relationship with other eating quality characteristics in rice. Japanese Journal of Crop Science, 1995, 27(3): 268-275.

[26]Toshiro H. Relationship between mineral element content and rice eating quality. Japanese Journal of Crop Science, 1990, 59: 605-611.

[27]周广洽, 徐孟亮, 李训贞. 生态因子对稻米蛋白质和氨基酸的影响. 生态学报, 1997, 17(5): 537-542.

Zhou G Q, Xu M L, Li X Z. Effects of ecological factors of protein and amino acids of rice. Acta Ecologica Sinica,1997, 17(5): 537-542. (in Chinese)