SCD1基因不同基因型的差异表达及与牛奶中脂肪酸组成的相关性

doi:10.3864/j.issn.0578-1752.2012.11.017

摘要/Abstract

摘要： 【目的】探索中国南方荷斯坦奶牛SCD1基因不同基因型的差异表达及与牛奶中脂肪酸组成的相关性。【方法】以同一奶牛养殖场的303头中国荷斯坦奶牛为样本，利用PCR-SSCP技术检测SCD1基因第5外显子878 bp位置的多态性，采用定量FQ-PCR技术初步检测3种基因型的相对表达量，并运用气相色谱技术分析牛奶中脂肪酸的组成。【结果】共检测到3种基因型，即CC、CT和TT，基因型频率分别为0.617、0.339和0.044，等位基因C和T的频率分别为0.787和0.213，仅发现CT表达量显著高于TT（P＜0.05）。CC、CT和TT在脂肪酸C4﹕0、C12﹕0、C17﹕0上存在显著差异（P＜0.05）；在脂肪酸C6﹕0、C8﹕0、C14﹕0、C15﹕0和c9t11CLA上，3种基因型间差异极显著（P＜0.01）。在总饱和脂肪酸含量上TT显著高于CC和CT（P＜0.05）。【结论】SCD1基因C878T位点的突变对中国荷斯坦牛SCD1基因不同基因型的表达和乳脂肪酸成分有较大的遗传效应。

关键词: 中国荷斯坦奶牛, SCD1基因, 基因型, 牛奶, 脂肪酸

Abstract: 【Objective】 The effect of SCD1 different genotypes on gene expression and milk fatty acid composition was studied. 【Method】 Three hundred and three Chinese Holstein cows of a dairy farm were used to detect the polymorphism of the exon 5 of SCD1 gene using PCR-SSCP, and the relative gene expression was assayed by quantitative FQ-PCR, the milk fatty acid composition was assayed using the method of gas chromatography.【Result】One SNP, C878T, was detected, defining three genotypes, CC, CT and TT, with frequencies of 0.617, 0.339 and 0.044, respectively. The allele frequencies of C and T were, respectively, 0.787 and 0.213. Only the expression of CT genotype was significantly higher than TT genotype (P＜0.05). Total saturated fatty acids of TT was significantly higher than CC or CT (P＜0.05). There were highly significant differences in the fatty acid C6:0, C8:0, C14:0, C15:0 and c9t11CLA (P＜0.01) , and significant differences in the fatty acid C4:0, C12:0, C17:0 among these three genotypes (P＜0.05).【Conclusion】The SNP C878T of SCD1 has a great genetic effect on the gene expression of SCD1 and the content of fatty acid in milk, it could be a useful genetic marker for milking trait in Chinese Holstein breeding.

Key words: Chinese Hosltein cow, SCD1 gene, genotype, milk, fatty acid

李云龙, 杨章平, 王小龙, 常玲玲, 冀德君, 刘姗, 甘宗辉. SCD1基因不同基因型的差异表达及与牛奶中脂肪酸组成的相关性[J]. 中国农业科学, 2012, 45(11): 2273-2279.

LI Yun-Long, YANG Zhang-Ping, WANG Xiao-Long, CHANG Ling-Ling, JI De-Jun, LIU Shan, GAN Zong-Hui. Genotype and Gene Expression of SCD1 Gene Associated with the Content and Composition of Fatty Acid in Milk[J]. Scientia Agricultura Sinica, 2012, 45(11): 2273-2279.

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参考文献

[1]Ha Y L, Grimm N K, Pariza M W. Newly recognized anticarcinogenic fatty acids: identification and quantification in natural and processed cheesese. Journal of Agricultural and Food Chemistry, 1989, 37(1): 75-81.

[2]Mele M, Conte G, Castiglioni B, Chessa S, Macciotta N P P, Serra A, Buccioni A, Pagnacco G, Secchiari P. Stearoyl-coenzyme a desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. Journal of Dairy Science, 2007, 90(9): 4458-4465.

[3]Campbell E M G, Gallagher D S, Davis S K, Taylor J F, Smith S B. Rapid communication: mapping of the bovine stearoyl-CoA desaturase (SCD) gene to BTA26. American Society of Animal Science, 2001, 79: 1945-1955.

[4]Jiang Z H, Jennifer J M, David J T, Tyler F D, Daniel C R, Michael D M. Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle. International Journal of Biological Sciences, 2008, 4(6): 345-351.

[5]Lengi A J, Corl B A. Identification and characterization of a novel bovine stearoyl-CoA desaturase isoform with homology to human SCD5. Lipids, 2007, 42(6): 499-508.

[6]Moioli B, Contarini G, Avalli A, Catillo G, Orrù L, de Matteis G, Masoero G, Napolitano F. Short communication: effect of stearoyl-coenzyme a desaturase polymorphism on fatty acid composition of milk. Journal of Dairy Science, 2007, 90(7): 3553-3558.

[7]李平. 利用牛乳汁体细胞研究乳腺葡萄糖转运和免疫相关因子的表达[D]. 郑州: 河南农业大学, 2009.

Li P. Use of somatic cell in bovine to study the expression of mammary glucose transport and immune-associated factors[D]. Zhengzhou: Henan Agricultural University, 2009. (in Chinese)

[8]Piotr C, Nicoletta S. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty- something years on. Nature Protocols, 2006, 1(2): 581-585.

[9]中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 乳与乳制品脂肪酸的测定—气相色谱法(GB/T21676- 2008). 北京: 中国标准出版社,2008.

General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China, Standardization Administration of the People’s Republic of China. Milk and Dairy Products-Determination of Fatty Acid Content-Gas-Liquid Chromatography (GB/T21676-2008). Beijing: Standards Press of China, 2008. (in Chinese)

[10]张驰宇, 徐顺高, 黄新祥. 一种新颖简便的荧光实时RT-PCR相对定量方法的建立. 生物化学与生物物理进展, 2005, 32(9): 883-887.

Zhang C Y, Xu S G, Huang X X. The construction of a new and easy real-time relative quantitative RT-PCR method. Progress in Biochemistry and Biophysics, 2005, 32(9): 883-887. (in Chinese)

[11]Taniguchi M, Utsugi T, Oyama K, Mannen H, Kobayashi M, Tanabe Y, Ogino A, Tsuji S. Genotype of stearoyl-CoA desaturase is associated with fatty acids composition in Japanese black cattle. Mammalian Genome, 2004, 15(2): 142-148.

[12]Komisarek J, Dorynek Z. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls. Journal of Applied Genetics, 2009, 50(2): 125-132.

[13]Macciotta N P P, Mele M, Conte G, Serra A, Cassandro M, Zotto R D, Borlino A C, Pagnacco G, Secchiari P. Association between a polymorphism at the stearoyl-CoA desaturase locus and milk production traits in Italian Holsteins. Journal of Dairy Science, 2008, 91: 3184-3189.

[14]Chung K Y, Lunt D K, Kawachi H, Yano H, Smith S B. Lipogenesis and stearoyl-CoA desaturase gene expression and enzyme activity in adipose tissue of short- and long-fed Angus and Wagyu steers fed corn- or hay-based diets. Journal of Animal Science, 2007, 85(2): 380-387.

[15]Soyeurt H, Dehareng F, Mayeres P, Bertozzi C, Gengler N. Variation of delta 9-desaturase activity in dairy cattle. Journal of Dairy Science, 2008, 91(8): 3211-3124.

[16]Siebert B D, Pitchford W S, Kruk Z A, Kuchel H, Deland M P B, Bottema C D. Differences in △9 desaturase activity between Jersey- and Limousin-sired cattle. Lipids, 2003, 38(5): 539-543.

[17]Beswick N S, Kennelly J J. Influence of bovine growth hormone and growth hormone-releasing factor on messenger RNA abundance of lipoprotein lipase and stearoyl-CoA desaturase in the bovine mam-mary gland and adipose tissue. Journal of Animal Science, 2007, 78(2): 412-419.

[18]Baumgard L H, Matitashvili E, Corl B A, Dwyer D A, Bauman D E. Trans-10, cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. Journal of Dairy Science, 2002, 85(9): 2155-2163.

[19]卜登攀, 王加启, 刘仕军, 刘晓云, 谷俊, 张瑞超. 奶牛日粮添加豆油抑制乳腺硬脂酰辅酶A去饱和酶(SCD)基因mRNA表达水平. 中国畜牧兽医, 2006, 33(7): 3-5.

Bu D P, Wang J Q, Liu S J, Liu X Y, Gu J, Zhang R C. Addition of soybean oil to diets for dairy cows decrease SCD gene expression in mammary gland. China Animal Husbandry and Veterinary Medicine, 2006, 33(7): 3-5. (in Chinese)

[20]Schennink A, Heck J M L, Bovenhuis H, Visker M H P W, Valenberg H J F V, Arendonk J A M V. Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and Acyl CoA: diacylglycerol acyltransferase 1 (DGAT1). Journal of Dairy Science, 2007, 91(5): 2135-2143.

[21]Kgwatalala P M, Ibeagha-Awemu E M, Mustafa A F, Zhao X. Influence of stearoyl-coenzyme A desaturase 1 genotype and stage of lactation on fatty acid composition of Canadian Jersey cows. Journal of Dairy Science, 2009, 92(3): 1220-1228.

[22]Orrù L, Cifuni G F, Piasentier E, Corazzin M, Bovolenta S, Moioli B. Association analyses of single nucleotide polymorphisms in the LEP and SCD1 geneson the fatty acid profile of muscle fat in Simmental bulls. Meat Science, 2011, 87(4): 344-348.

[23]Signorelli F, Orrù L, Napolitano F, Matteis G D, Scatà M C, Catillo G, Marchitelli C, Moioli B. Exploring polymorphisms and effects on milk traits of the DGAT1, SCD1 and GHR genes in four cattle breeds. Livestock Science, 2009, 125(1): 74-79.