中国农业科学 ›› 2012, Vol. 45 ›› Issue (1): 44-51.doi: 10.3864/j.issn.0578-1752.2012.01.006

• 耕作栽培·生理生化·农业信息技术 • 上一篇    下一篇

细胞分裂素对水稻分蘖芽生长及分蘖相关基因表达的调控

 刘杨, 顾丹丹, 许俊旭, 丁艳锋, 王强盛, 李刚华, 刘正辉, 王绍华   

  1. 1.南京农业大学农学院/农业部南方作物生理生态重点开放实验室,南京210095
    2.西北农林科技大学农学院,陕西杨凌 712100
  • 收稿日期:2011-06-13 出版日期:2012-01-01 发布日期:2011-09-21
  • 通讯作者: 通信作者王绍华,Tel:025-84396475;E-mail:wangsh@njau.edu.cn
  • 作者简介:刘 杨,E-mail:liuyang0328@126.com
  • 基金资助:

    国家自然科学基金项目(31071364)、江苏省自然科学基金项目(BK2010449)

Effect of Cytokinins on the Growth of Rice Tiller Buds and the Expression of the Genes Regulating Rice Tillering

 LIU  Yang, GU  Dan-Dan, XU  Jun-Xu, DING  Yan-Feng, WANG  Qiang-Sheng, LI  Gang-Hua, LIU  Zheng-Hui, WANG  Shao-Hua   

  1. 1.南京农业大学农学院/农业部南方作物生理生态重点开放实验室,南京210095
    2.西北农林科技大学农学院,陕西杨凌 712100
  • Received:2011-06-13 Online:2012-01-01 Published:2011-09-21

摘要: 【目的】研究细胞分裂素(CTK)和分蘖相关基因表达在调控水稻分蘖芽生长过程中的相互关系,探讨水稻分蘖芽生长的分子机理。【方法】以南粳44为材料,利用氮(N)和生长素(IAA)调控水稻分蘖芽萌发,分析OsTB1、OsD3、OsD10和OsD27的表达和CTK含量的变化。【结果】外源40 mg•L-1N增加了分蘖节和分蘖芽中CTK含量,抑制了分蘖芽中OsTB1、OsD3、OsD10和OsD27的表达,促进了分蘖芽的萌发生长,外源喷施IAA则逆转了氮的作用。分蘖芽中OsTB1的表达受CTK调控,而OsD3、OsD10和OsD27的表达可能不受CTK调控。【结论】外界因素对分蘖芽生长的调控至少存在2条途径,一条是通过调控节和芽中CTK含量,进而调控芽中OsTB1等基因表达;另一条通过调控OsD3、OsD10和OsD27的表达。

关键词: 水稻, 细胞分裂素, 分蘖芽, 分蘖相关基因

Abstract: 【Objective】 The objective of the present study was to investigate the relationship of cytokinins (CTK) and the expression of the genes regulating rice tillering during the regulating the growth of rice tiller buds growth and the underlying molecular mechanisms. 【Method】 In the present study, a cultivar Nanjing 44 was used. At tillering stage, 40 mg?L-1 nitrogen (N) was used to stimulate the germination of tiller buds. At the same time, external IAA was used to inhibit the germination of tiller buds. The changes of CTK contents in tiller buds and tiller nodes and the expression of OsTB1, OsD3, OsD10 and OsD27 in tiller buds were measured. 【Result】 The external 40 mg?L-1 N increased the CTK contents in tiller nodes and tiller buds, inhibited the expression of OsTB1, OsD3, OsD10 and OsD27 in tiller buds, and then promoted the growth of rice tiller buds. However, the external application of IAA reversed the effect of N. Besides this, the expression of OsTB1 in tiller buds was regulated by CTK, however, the expression of OsD3, OsD10 and OsD27 in tiller buds might not be regulated by CTK. 【Conclusion】 The results showed that external N and IAA might have regulated tiller bud growth by at least two means: one is regulating the CTK content and the expression level of OsTB1 in tiller buds, another is regulating the expression level of OsD3, OsD10 and OsD27 in tiller buds and by this to regulate the tiller bud growth.

Key words: rice, cytokinin, tillerbud, genesregulatingtillering