玉米秸秆发酵垫料微生物对小鼠免疫功能的影响

doi:10.3864/j.issn.0578-1752.2011.16.020

中国农业科学 ›› 2011, Vol. 44 ›› Issue (16): 3463-3468.doi: 10.3864/j.issn.0578-1752.2011.16.020

玉米秸秆发酵垫料微生物对小鼠免疫功能的影响

高金波, 牛钟相

1. 山东农业大学动物医学院
2. 山东农业大学动物科技学院/动物生物技术与疫病防治重点实验室

收稿日期:2010-10-11 修回日期:2011-03-17 出版日期:2011-08-15 发布日期:2011-04-14
通讯作者: 通信作者牛钟相，E-mail：zxniu@sdau.edu.cn
作者简介:高金波，E-mail：jinbogao1985@163.com
基金资助:
国家“十一五”科技支撑计划项目（2006BAD06A11）

Effects of Microbial Fermentation of Corn Straw Mattress Material on Immunological Function of Mice

GAO Jin-Bo, NIU Zhong-Xiang

1. 山东农业大学动物医学院
2. 山东农业大学动物科技学院/动物生物技术与疫病防治重点实验室

Received:2010-10-11 Revised:2011-03-17 Online:2011-08-15 Published:2011-04-14
Contact: Jin-Bo GAO

摘要/Abstract

摘要： 【目的】以小白鼠为实验动物模型、玉米秸秆为发酵床垫料，探讨发酵床垫料中的微生物对小鼠机体免疫功能的影响。【方法】将小鼠随机分为4组，第1—3组小鼠分别置于用不同微生物配方发酵的玉米秸秆垫料中饲养，第4组作为对照组，放入未发酵的垫料中饲喂。期间检测小鼠血清中的抗体效价、IL-4和IFN-γ的含量，血液中白细胞总数、淋巴细胞数量、红细胞总数及小鼠盲肠内乳酸菌数量，抗疲劳游泳时间等指标。【结果】发酵垫料组微生物能显著提高小鼠抗体效价和血液中红细胞数量、血红蛋白浓度及盲肠乳酸菌数量（P＜0.05）；可增加血清中IFN-γ、IL-4含量和血液中白细胞总数、淋巴细胞数量，差异不显著（P＞0.05）；抗疲劳试验结果表明，发酵垫料微生物能显著延长小鼠的游泳时间（P＜0.05）。【结论】玉米秸秆发酵垫料微生物可显著提高动物体的免疫功能及健康状况。

关键词: 发酵玉米秸秆, 微生物, 小鼠, 免疫功能, 抗体, 细胞因子

Abstract: 【Objective】 The aim of this experiment was to study the effect of microbes in fermented corn stalk on immunologic function of mice.【Method】 Forty mice at 4-week-old of age were divided into four groups randomly and raised to establish different kinds of fermention corn stalk with microbes group as well as control group (without addition of microbes). In the experiments, the antibody titer, contents of IL-4 and IFN-γ in serum, the total number of white blood cell (WBC), lymphocyte (LYM) and red blood cell (RBC), and mean corpuscular haemoglobin concentration (MCHC) in blood were determined. The count of Lactobacillus in cecal content and the anti-fatigue time of swimming of mice were examined.【Result】The results showed that microbes in fermented corn stalk significantly promoted the level of antibody and the count of Lactobacillus in cecal content, RBC and MCHC in blood (P＜0.05). The WBC and LYM in blood, the content of IFN-γ, IL-4 were also increased (P＞0.05). The result of anti-fatigue experiment showed that the microbes in fermented corn stalk significantly increased the time of swimming of mice (P＜0.05). 【Conclusion】 Microbes in fermented corn stalk can significantly improve the immune function of mice and other indicators.

Key words: fermention corn stalk, microbes, mice, immunologic function, antibody, cytokines

高金波, 牛钟相. 玉米秸秆发酵垫料微生物对小鼠免疫功能的影响[J]. 中国农业科学, 2011, 44(16): 3463-3468.

GAO Jin-Bo, NIU Zhong-Xiang. Effects of Microbial Fermentation of Corn Straw Mattress Material on Immunological Function of Mice[J]. Scientia Agricultura Sinica, 2011, 44(16): 3463-3468.

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参考文献

[1]Tiquia S M, Tam N F Y, Hodgkiss I J. Microbial activities during composting of spent pig-manure sawdust litter at different moisture contents. Bioresource Technology, 1996, 55: 201-206.

[2]杨朝飞. 加强禽畜粪便污染防治迫在眉睫. 生态与自然保护, 2001, 2: 33-35.

Yang C F. Pollution control of livestock breeding wastes is extremely urgent. Ecology and Natural Conservation, 2001, 2: 33-35. (in Chinese)

[3]Tiquia S M, Tam N F Y, Hodgkiss I J. Effects of bacterial inoculum and misture adjustment on composting of pig manure. Environmental Pollution, 1997, 96(2): 161-171.

[4]李荣林, 杨胜林, 何勇. 发酵床养猪及常规养猪的对比分析. 贵州农业科学, 2009, 37(11): 122-124.　

Li R L, Yang S L, He Y. Comparative analysis of pig raising between fermenting bed and conventional method. Guizhou Agricultural Sciences, 2009, 37(11): 122-124. (in Chinese)

[5]王远孝, 王　恬. 发酵床养猪技术的应用研究进展. 动物医学进展, 2010, 31: 193-196.

Wang Y X, Wang T. Progress on and application of biology bed in pigs raising. Progress in Veterinary Medicine, 2010, 31: 193-196. (in Chinese)

[6]Tam N F Y, Vrijmoed L L P. Effects of commercial bacterial products on nutrient transformations of pig manure in a pig-on-littersystem. Water Management and Research, 1990, 8(5): 363-373.

[7]Chan D K O, Chaw D, Lo C Y Y. Management of the sawdust litter in the ‘pig-on-litter’ system of pig waste treatment. Resources, Conservation and Recycling, 1994(11): 51-72.

[8]Morrison R S, Hemsworth P H, Cronin G M, Campbell R G. The social and feeding behaviour of growing pigs in deep-litter, group housing systems. Applied Animal Behaviour Science, 2003(82): 173-188.

[9]Karlen G A M, Hemsworth P H, Gonyou H W, Fabrega E, Strom A D, Smits R J. The welfare of gestating sows in conventional stalls and large groups on deep litter. Applied Animal Behaviour Science, 2007, 105(3): 87-101.

[10]朱洪, 常志州, 叶小梅, 费辉盈. 基于畜禽废弃物管理的发酵床技术研究:Ⅲ高湿热季节养殖效果评价. 农业环境科学学报, 2008, 27(1): 354-358.

Zhu H, Chang Z Z, Ye X M, Fei H Y. Study on deep litter system for management of livestock manure evaluation on effects of deep litter system on pig growth in high temperature and RH season. Journal of Agro-Environment Science, 2008, 27(1): 354-358. (in Chinese)

[11]武华玉, 乔木, 郭万正, 彭先文, 宋忠旭, 郭锐, 梅书棋. 生物发酵床养猪效果研究. 湖北农业科学, 2009, 48(12): 3090-3094.

Wu H Y, Qiao M, Guo W Z, Peng X W, Song Z X, Guo R, Mei S Q. Study on the effect of raising pig on biological fermentation bed. Hubei Agricultural Sciences, 2009, 48(12): 3090-3094. (in Chinese)

[12]何明清, 程安春. 动物微生态学. 成都: 四川科技出版社, 2004.

He M Q, Cheng A C. Animal Microecology. Chengdu: Sichuan Science and Technology Press, 2004. (in Chinese)

[13]龙碧波. 海南野生绞股蓝抗运动性疲劳的实验研究. 现代预防医学, 2009, 36(4): 712-716.

Long B B. Experimental research on the anti-fatigue effect of extraction of gynostemma pen taphyllum. Modern Preventive Medicine, 2009, 36(4): 712-716. (in Chinese)

[14]Reiner S L, Seder R A. T helper cell differentiation in immune response. Current Opinion in Immunology, 1995, 7(3): 360-366.

[15]Guo L Y, Jane H L, William E. Probabilistic regulation of IL-4 production in Th2 cells: accessibility at the IL-4 locus. Immunity, 2004, 20: 193-203.

[16]Sun P, Wang J Q, Zhang H T. Effects of bacillus subtilis natto on performance and immune function of preweaning calves. Journal of Dairy Science, 2010, 12(93): 5851-5855.

[17]Sakka K, Kimura T, Karita S, Ohmiya K. Molecular breeding of cellulolytic microbes. Journal of Bioscience and Bioengineering, 2000, 90(3): 227-233.

[18]Guerra N P, Bernárdez P F, Castro L P. Production of four potentially probiotic lactic acid bacteria and their evaluation as feed additives for weaned piglets. Animal Feed Science and Technology, 2007(134): 89-107.

[19]Scharek L, Altherr B J, Tölke C. Influence of the probiotic Bacillus cereus var. Toyoi on the intestinal immunity of piglets. Veterinary Immunology and Immunopathology, 2007, 120(3-4): 136-147.

[20]Suzuki D, Furukawa K, Kimura F, Shimizu H, Yoshidome H, Ohtsuka M, Kato A, Yoshitomi H, Miyazaki M. Effects of perioperative immunonutrition on cell-mediated immunity, T helper type 1(Th1)/ Th2 differentiation and Th17 response after pancreaticoduodenectomy. Surgery, 2010, 148(3): 573-581.

[21]Dlugovitzky D, Fiorenza G, Farroni M, Bogue C, Stanford C, Stanford J. Immunological consequences of three doses of heat-killed mycobacterium vaccae in the immunotherapy of tuberculosis. Respiratory Medicine, 2006, 100(6): 1079-1087.

[22]Christos D. Nonsymbiotic hemoglobins and stress tolerance in plants. Plant Science, 2009, 176(4): 433-440.

[23]Siegel I, Liu T L, Gleicher V. The red cell immune system. The Lancet, 1981(2): 556-559.

[24]Watanabe T, Takeda T, Omiya S, Hikoso S, Yamaguchi O, Nakano Y, Higuchi Y, Nakai A, Abe Y, Aki-Jin Y, Taniike M, Mizote I, Matsumura Y, Shimizu T, Nishida K, Imai K, Hori M, Shirasawa T, Otsu K. Reduction in hemoglobin-oxygen affinity results in the improvement of exercise capacity in mice with chronic heart failure. Journal of the American College of Cardiology, 2008, 52(9): 779-786.

[25]Hoy J A, Hargrove M S. The structure and function of plant hemoglobins. Plant Physiology and Biochemistry, 2008, 46(3): 371-379.

[26]何明清, 程安春. 动物微生态学. 成都: 四川科技出版社, 2004.

He M Q, Cheng A C. Animal Microecology. Chengdu: Sichuan Science and Technology Press, 2004. (in Chinese)

[27]Seegers F M L. Lactobacilli as live vaccine delivery vectors:progress and prospects. Trends Biotechnology, 2002, 20(12): 508-515.

玉米秸秆发酵垫料微生物对小鼠免疫功能的影响

Effects of Microbial Fermentation of Corn Straw Mattress Material on Immunological Function of Mice

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