草莓,水杨酸,铁,叶绿素,过氧化氢酶,过氧化物酶,顺乌头酸酶," /> 草莓,水杨酸,铁,叶绿素,过氧化氢酶,过氧化物酶,顺乌头酸酶,"/> strawberry,salicylic acid,iron,chlorophyll,catalase,peroxidase,aconitase
,"/> <font face="Verdana">水杨酸和铁在调节草莓组培苗生长中的关系</font>

中国农业科学 ›› 2010, Vol. 43 ›› Issue (13): 2751-2758 .doi: 10.3864/j.issn.0578-1752.2010.13.015

• 园艺 • 上一篇    下一篇

水杨酸和铁在调节草莓组培苗生长中的关系

李淑平,原永兵,刘更森,刘成连,王永章,李培环#br#   

  1. (青岛农业大学园林园艺学院/青岛市现代农业质量与安全工程重点实验室)
  • 收稿日期:2010-01-29 修回日期:2010-03-29 出版日期:2010-07-01 发布日期:2010-07-01
  • 通讯作者: 原永兵

Relationship Between Salicylic Acid and Iron in Their Regulations of the Growth of Strawberry Tissue Culture Plants#br#

LI Shu-ping,YUAN Yong-bing, LIU Geng-sen, LIU Cheng-lian, WANG Yong-zhang, LI Pei-huan#br#   

  1. (青岛农业大学园林园艺学院/青岛市现代农业质量与安全工程重点实验室)
  • Received:2010-01-29 Revised:2010-03-29 Online:2010-07-01 Published:2010-07-01
  • Contact: YUAN Yong-bing

摘要:

【目的】探讨水杨酸(SA)和铁在调控植物生长中的关系,以期进一步揭示SA的作用机理。【方法】本研究以草莓组培苗为试材,用0.05—0.8 mmol?L-1 系列浓度的SA处理生长于含0.05—0.8 mmol?L-1 FeSO4培养基上的草莓组培苗,通过草莓苗的生长状况和过氧化氢酶(catalase, CAT)、过氧化物酶(peroxidase, POD)及顺乌头酸酶(aconitase, ACO)3种含铁酶的活性,分析SA和铁的相互作用。【结果】低浓度铁培养的草莓苗对SA的反应比高浓度铁敏感,SA处理的最适宜浓度随铁浓度的升高而升高。SA抑制根的生长;低浓度SA增加株高和叶绿素含量,高浓度SA抑制植株生长,降低叶绿素含量。0.4 mmol?L-1SA处理对组培苗造成伤害,但随FeSO4浓度(0.05—0.4 mmol?L-1)的增加,根长、株高和叶绿素含量增加,存活率增加。0.05—0.2 mmol?L-1SA增加CAT和ACO的活性,0.4 mmol?L-1SA降低其活性;0.05—0.4 mmol?L-1SA提高POD的活性。【结论】水杨酸调节草莓组培苗生长的过程和铁有密切关系。

关键词: 草莓')">草莓, 水杨酸, 铁, 叶绿素, 过氧化氢酶, 过氧化物酶, 顺乌头酸酶

Abstract:

【Objective】 In order to explain the mechanism on the roles of salicylic acid in plant physiology and development, tissue culture plants of strawberry were used to explore the possible interactions between SA and iron. 【Method】 SA at a series of concentrations (0.05-0.8 mmol?L-1 ) was applied to the medium with a series of concentrations (0.05-0.8 mmol?L-1 ) of iron. Plant growth and the activities of some iron enzymes were measured. 【Result】 The plants were shown to be more sensitive to SA in the medium with lower concentrations of iron than higher ones. The optimal concentrations of SA for plant growth increased gradually along with the increase of iron concentrations in the medium. SA usually inhibited root elongation. Low concentrations of SA increased the plant height and chlorophyll content while high concentrations of SA reduced these indices. Higher concentration of SA at 0.4 mmol?L-1 injured the plants, but such injury became less with the increase of the concentration of iron. The activities of CAT and ACO were enhanced by SA at the concentrations of 0.05-0.2 mmol?L-1 while reduced at 0.4 mmol?L-1. POD activity was increased by SA at 0.05-0.4 mmol?L-1. 【Conclusion】 SA and iron have close relationship in regulating the growth of strawberry.

Key words: strawberry')">strawberry, salicylic acid, iron, chlorophyll, catalase, peroxidase, aconitase