中国农业科学 ›› 2009, Vol. 42 ›› Issue (12): 4383-4389 .doi: 10.3864/j.issn.0578-1752.2009.12.033

• 研究简报 • 上一篇    下一篇

不同外源激素对花铃期棉花主茎叶生理特性的影响

郑莎莎,孙传范,孙红春,刘连涛,赵金峰,李存东

  

  1. (河北农业大学农学院/河北省作物生长调控重点实验室)
  • 收稿日期:2009-03-12 修回日期:2009-05-25 出版日期:2009-12-10 发布日期:2009-12-10
  • 通讯作者: 李存东

Effects of Different Exogenous Hormones on Physiological Characteristics of Main Stem Leaves at Flower and Boll Stage in Cotton

ZHENG Sha-sha, SUN Chuan-fan, SUN Hong-chun, LIU Lian-tao, ZHAO Jin-feng, LI Cun-dong
  

  1. (河北农业大学农学院/河北省作物生长调控重点实验室)
  • Received:2009-03-12 Revised:2009-05-25 Online:2009-12-10 Published:2009-12-10
  • Contact: LI Cun-dong

摘要:

【目的】研究初花期(7月6日)、盛花期(7月17日)喷施不同浓度GA3(赤霉素)、BR(油菜素内酯)和6-BA(6苄基腺嘌呤)对棉花盛花期(7月17日)和盛铃期(7月31日)主茎功能叶生理特性的影响。【方法】试验设GA3、BR和6-BA 3种激素处理,每种激素设2种浓度,即GA3(10和30 mg•L-1)、BR(0.01和0.1 mg•L-1)和6-BA(10和100 mg•L-1),以喷清水为对照。测定主茎功能叶内的可溶性糖、丙二醛、叶绿素、可溶性蛋白质、内源激素含量,SOD(超氧化物歧化酶)和POD(过氧化物酶)活性。【结果】100 mg•L-1的6-BA能够显著提高盛花期主茎功能叶叶绿素、可溶性蛋白质含量和SOD、POD活性,使MDA(丙二醛)含量显著降低。100 mg•L-1的6-BA和30 mg•L-1的GA3处理,使盛花期内源ZR(玉米素核苷)、GA3含量和ZR/ABA(脱落酸)、GA3/ABA、(ZR+GA3)/ABA值均显著高于对照。0.01 mg•L-1的 BR和10 mg•L-1的GA3处理,盛花期主茎功能叶生理活性也有所提高。100 mg•L-1的6-BA和30 mg•L-1的GA3处理也显著提高了棉花盛铃期主茎功能叶可溶性蛋白质含量、GA3/ABA和GA3+ZR/ABA值,并使丙二醛含量显著降低。【结论】在本试验条件下,叶面喷施100 mg•L-1的6-BA、30 mg•L-1的GA3能够显著提高棉花花铃期主茎功能叶的生理活性,为生产中采用生长促进类外源激素防止棉花过早衰老提供了依据。

关键词: 棉花, 花铃期, 主茎叶, 外源激素, 生理特性

Abstract:

【Objective】 The effects of spraying exogenous hormones(gibberellin-3, GA3; brassinolide, BR and 6-benzylaminopurine, 6-BA) at early (July 6) and full (July 17) flowering time on physiological characteristics of main stem functional leaves at flower and boll stage. 【Method】 Three exogenous hormone treatments were emploid, with two concentration levels each, i.e. GA3 10 and 30 mg•L-1, BR 0.01 and 0.1 mg•L-1, and 6-BA 10 and 100 mg•L-1,with spraying water as the control(CK). The contents of chlorophyll, soluble protein, malondialdehyde, soluble sugar, endogenous hormone contents and the activities of superoxide dismutase (SOD) and peroxidase (POD) were measured. 【Result】 The results showed that the treatment of 100 mg•L-1 6-BA significantly increased the contents of chlorophyll, soluble protein, soluble sugar and the activities of SOD and POD at full flowering stage. The treatments of 6-BA 100 mg•L-1 and GA3 30 mg•L-1 significantly increased the contents of GA3 and ZR, and the ratio of ZR (zeatin riboside) / ABA (abscisic acid ),GA3 /ABA, and (ZR+GA3)/ABA at the same stage. The content of MDA greatly decreased. The treatments of BR (0.01 mg•L-1) and GA3 (10 mg•L-1) also had a positive effect, but the effect was weaker. The treatments of GA3 30 mg•L-1 and 6-BA 100 mg•L-1 significantly increased the content of soluble protein, the ratio of GA3/ABA, GA3+ZR/ABA, while the content of MDA significantly decreased at full bolling stage.【Conclusion】 Under this experimental condition, spraying 6-BA 100 mg•L-1 and GA3 30 mg•L-1 had significantly effects on the physiological functions of main stem functional leaves, it could be used for delaying the senescence of cotton.

Key words: cotton, flower and boll stage, stem leaves, exogenous hormones, physiology characteristics