中国农业科学 ›› 2009, Vol. 42 ›› Issue (11): 3812-3818 .doi: 10.3864/j.issn.0578-1752.2009.11.007

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

谷子品种资源微量元素硒和蛋白质含量的测定与评价

刘三才,朱志华,李为喜,刘方,李燕,黄蓉

  

  1. (中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物品种资源监督检验测试中心)
  • 收稿日期:2009-03-20 修回日期:2009-04-13 出版日期:2009-11-10 发布日期:2009-11-10

Evaluation of Selenium and Protein Content of Foxtail Millet Landraces Originated from Different Ecogical Regions of China

LIU San-cai, ZHU Zhi-hua, LI Wei-xi, LIU Fang, LI Yan, HUANG Rong
  

  1. (中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物品种资源监督检验测试中心)
  • Received:2009-03-20 Revised:2009-04-13 Online:2009-11-10 Published:2009-11-10

摘要:

【目的】对中国谷子地方品种资源进行硒和蛋白质含量的测定与评价,以了解谷子品种资源中硒和蛋白质含量的变异及其与地理分布的关系,为谷子种质创新和育种提供基础。【方法】硒和蛋白质含量测定分别采用氢化物原子荧光法和微量凯氏定氮法。【结果】①中国谷子地方品种资源硒含量平均53.3 μg?kg-1,变异范围20.8~89.2 μg?kg-1;蛋白质含量平均15.8%,变异范围11.01%~20.77%。②不同生态区的材料硒和蛋白质含量存在明显差异,西北内陆和黄土高原来源的品种硒含量分别排列第一和第二,以下依次是东北平原、华北平原,最低是内蒙古高原;不同生态区来源的品种蛋白质含量排列次序与硒的排列较为一致,依次为西北内陆、黄土高原、华北平原、东北平原和内蒙古高原。③2个品质性状的相关分析表明,硒和蛋白质含量存在一定的正相关性,相关系数为0.534,且达到极显著水平。④硒含量与谷子粒色相关联,相联系数为0.385,达到0.05的显著水平,红粒谷子硒含量明显高于黄、青、褐等粒色的品种。【结论】谷子品种资源的硒和蛋白质含量存在较为丰富的遗传变异,在鉴定评价基础上,筛选出了一批品质优异的谷子种质资源,为富硒和高蛋白优质品种的培育和种质创新奠定了基础。

关键词: 谷子, 微量元素, 硒含量, 蛋白质含量, 相关分析, 粒色

Abstract:

【Objective】 Selenium and protein contents were analyzed and evaluated in foxtail millet landraces originated from different regions of China. 【Method】 The method for selenium determination was Hydride Generation Atomic Fluorescence Spectrometry, and the method for protein determination was semi-micro Kjeldahl method. 【Result】 ① The average content of selenium in Chinese foxtail millet landraces was 53.3 μg?kg-1, deviation range was 20.8-89.2 μg?kg-1. The average content of protein was 15.82%, deviation range was 11.01%-20.77%. ② Selenium and protein content in different ecoregions in China appeared to be greatly diversified, landraces from Northwest China had the highest selenium content, and the followings were landraces from Loess Plateau, North China Plain, Northeast China Plain, and landraces from the Inner Mongolia Plateau had the lowest content. The protein content ranking was alike the selenium content and that from high content to low were landraces from Northwest China, Loess Plateau, Northeast China Plain, Northeast China Plain, and Inner Mongolia Plateau. ③ The correlation coefficient analysis between the two traits showed that they existed a positive correlation, and the coefficient was 0.534 which was at the significant level. ④ The correlation coefficient of selenium content to hull colour was 0.385 and selenium content of red hull seed was greater that of the yellow, grey, brown hull. 【Conclusion】 It was found from the study that selenium and protein contents in foxtail millet landraces germplasms exhibited wide genetic diversity, some foxtail millet landraces with higher selenium and protein contents were identified and they will be useful for quality breeding and germplasm innovation.

Key words: foxtail millet, trace element, selenium content, protein content, correlation analysis, hull colour