中国农业科学 ›› 2009, Vol. 42 ›› Issue (3): 974-981 .doi: 10.3864/j.issn.0578-1752.2009.03.028

• 园艺 • 上一篇    下一篇

菊花耐寒特性分析及其评价指标的确定

  

  1. 南京农业大学园艺学院
  • 收稿日期:2008-03-23 修回日期:2008-06-12 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 陈发棣

Analysis of Cold-Tolerance and Determination of Cold-Tolerance Evaluation Indicators in Chrysanthemum

  1. 南京农业大学园艺学院
  • Received:2008-03-23 Revised:2008-06-12 Online:2009-03-10 Published:2009-03-10
  • Contact: CHEN Fa-di

摘要:

【目的】低温是影响菊花生长的主要环境因子之一,从生理、形态解剖等水平对菊花耐寒特性进行研究,并探讨各耐寒指标之间的关系,建立可靠的菊花耐寒性数学评价模型,为菊花耐寒种质的挖掘、耐寒性新品种选育及大规模菊花品种的耐寒评价奠定基础。【方法】对6个菊花品种在低温处理下的脚芽叶片的SOD酶、CAT酶、MDA、可溶性糖和可溶性蛋白等生理指标以及解剖结构特征的分析,结合相关性检验,筛选与耐寒性密切相关的指标。运用主成分分析、回归分析、聚类分析和通径分析方法,对筛选指标进行综合分析。【结果】对14个与耐寒性有关的指标进行了分析,结果发现菊花半致死温度(LT50)与-14~-17℃的SOD变化率、-11~-14℃的可溶性糖变化率(SS1)、栅栏组织厚度(L2)和上表皮厚度(L4)、栅/海比(L6)、栅栏组织紧密度(L7)呈极显著负相关(P<0.01),与栅栏组织疏松度(L8)呈显著正相关。【结论】提炼出4个指标并建立了菊花耐寒性评价的数学模型:Y=16.370-0.029SS1-0.075L7-0.314L8-12.250L6(R2=1.000),预测精度大于94%。

关键词: 菊花, 耐寒性, 生理, 解剖结构, 评价

Abstract:

【Objective】 Low temperature is one of the most important factors affecting the growth of chrysanthemum. To evaluate the cold tolerance of chrysanthemum, physiological and anatomical characteristics in chrysanthemum were studied under low temperature, the relationship between different indexes of cold tolerance were revealed, and mathematical evaluation model on cold tolerance was established too, which will provide a basis for cold tolerant germplasm discovery, breeding of new cold-tolerant varieties and evaluation on cold tolerant of a large number of chrysanthemum varieties. 【Method】 Physiological parameters such as SOD activity, CAT activity, content of MDA, soluble sugar and soluble protein and anatomical structure of leaves of rhizomes were studied in six chrysanthemum cultivars. Several indexes closely related to cold tolerance were screened out via correlation test. The validity of those screened indexes were further verified by main component analysis, regression analysis, hierarchical cluster analysis and path analysis. 【Result】 Analysis of related indexes with cold tolerance showed that significant negative correlations (P<0.01) were presented and changing rate of SOD activity at -14℃ to -17℃ , soluble sugar content (SS1) at -11℃ to -14℃, thickness of palisade tissue (L2), thickness of upper epidermis cells (L4), and ratio of palisade tissue against spongy tissue (L6), compactness of palisade tissue (L7) were observed, while a significant positive correlation (P<0.01) between LT50 and looseness of palisade tissue (L8) was found. 【Conclusion】 Four indexes closely related to the cold tolerance were screened. Mathematical evaluation model for chrysanthemum cold tolerance, i.e., Y=16.370-0.029SS1-0.075L7-0.314L8-12.250L6 (R2=1.000), was established too, and the predicting accuracy of the model is higher than 94%.

Key words: chrysanthemum, cold tolerance, physiological characteristics, anatomical structure, evaluation