关键词: 普通小麦, 蛋白质含量, 和面时间, 8分钟带宽, 峰值粘度, 稀懈值, QTL

Abstract: Identification of QTL for wheat processing quality traits and their linked molecular markers is very important for quality improvement with marker-assisted selection. In the present study, a total of 240 F5:6 lines from the cross PH82-2/Neixiang 188 were planted in a Latinized alpha-lattice design in Jiaozuo and Anyang of He’nan Province and Taian of Shandong Province, respectively. Grain and flour protein contents, Zeleny sedimentation value, Mixograph and RVA parameters were evaluated. A genetic map was constructed based on 188 SSRs and 4 protein markers. QTL analysis was conducted with the software QTL Cartographer 2.5. Four QTLs were detected for kernel protein content on chromosomes 3A, 3B and 4B, explaining 5.5％, 8.1％, 4.4％ and 5.1％ of the phenotypic variance, respectively. One QTL was detected for flour protein content on chromosome 4B, accounting for 7.0% of phenotypic variance. Three QTL were found for Zeleny sedimentation value on chromosomes 1B, 1D and 3B, in which the QTLs on chromosome 1B and 1D were detected both in Taian and Jiaozuo, explaining 10.4％~14.6％ of phenotypic variance. Three QTLs for mixing time were mapped on chromosomes 1B and 1D across three environments, accounting for 6.0％~55.3％ of phenotypic variance. Five QTLs were detected for eight minute width on chromosomes 1B, 1D and 4B. One common QTL on chromosome 1D was found in all three environments explaining 20.2%, 6.2% and 33.9% of phenotypic variance, respectively. Two QTLs on chromosome 1B were found both in Taian and Anyang, accounting for 5.2％~14.1％ of phenotypic variance,,Three QTLs For peak viscosity were detected on chromosomes 1A, 3A and 7B. Five QTLs for breakdown were found on chromosomes 1B, 4A, 5B, 6B and 7A, explaining 5.1％~7.3％ of phenotypic variance. The QTLs controlling Zenely sedimentation value, mixing time, eight minute width and breakdown were mapping on the same region of chromosome 1B, with genetic distances of 0.1~0.8 cM from the nearest marker Glu-B3j, indicating the big influences of 1BL/1RS translocation on these traits. In addition, on chromosome 1D, one QTL was found to control the Zeleny sedimentation value, mixing time and eight minute width, with genetic distances of 2.5~3.3 cM from the nearest marker Dx5+Dy10, exhibiting the great effect of HMW glutenin subunits Dx5+Dy10 on these traits. The QTLs for Zeleny sedimentation value and kernel protein content on chromosome 3B were also mapped on the same location. On chromosome 4B, the QTL for kernel and flour protein contents were found on the same region. The QTL for mixing time on chromosome 1B and the one for eight minute width on chromosome 1D were detected across three environments, with stable effects on the traits. The identified molecular markers related to the quality traits in this study will benefit for marker-assisted selection in breeding programs.