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| Cloning and characterization of a novel UDP-glycosyltransferase gene induced by DON from wheat |
| MA Xin, DU Xu-ye, LIU Guo-juan, YANG Zai-dong, HOU Wen-qian, WANG Hong-wei, FENG De-shun |
| State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China |
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摘要 Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a destructive disease of wheat throughout the world. However, the mechanisms of host resistance to FHB are still largely unclear. Deoxynivalenol (DON) produced by F. graminearum which enhances the pathogen to spread could be converted into inactive form D3G by UDP-glycosyltransferases (UGTs). A DON responsive UGT gene, designated as TaUGT4, was first cloned from wheat in this study. The putative open reading frame (ORF) of TaUGT4 was 1 386 bp, encoding 461 amino acids protein. TaUGT4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring (CS). When fused with eGFP at C terminal, TaUGT4 was shown to localize in cytoplasm of the transformed tobacco cells. The transcriptional analysis revealed that TaUGT4 was strongly induced by F. graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199, especially in Sumai 3 under DON treatment. Similar increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid (SA) treatment. But interestingly, the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate (MeJA). According to the expression patterns, TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F. graminearum inoculation. It had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against F. graminearum compared to SA signaling pathway.
Abstract Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a destructive disease of wheat throughout the world. However, the mechanisms of host resistance to FHB are still largely unclear. Deoxynivalenol (DON) produced by F. graminearum which enhances the pathogen to spread could be converted into inactive form D3G by UDP-glycosyltransferases (UGTs). A DON responsive UGT gene, designated as TaUGT4, was first cloned from wheat in this study. The putative open reading frame (ORF) of TaUGT4 was 1 386 bp, encoding 461 amino acids protein. TaUGT4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring (CS). When fused with eGFP at C terminal, TaUGT4 was shown to localize in cytoplasm of the transformed tobacco cells. The transcriptional analysis revealed that TaUGT4 was strongly induced by F. graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199, especially in Sumai 3 under DON treatment. Similar increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid (SA) treatment. But interestingly, the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate (MeJA). According to the expression patterns, TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F. graminearum inoculation. It had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against F. graminearum compared to SA signaling pathway.
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Received: 03 April 2014
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (31071405 and 31171553), the National High-Tech R&D Program of China (863 Program, 2011AA100102 and 2012AA101105), the Transgenic Special Item, China (2011ZX08002-004 and 2011ZX08009-003), the International Collaboration Program (948 Project, 2013- S19), the Doctoral Fund of Ministry of Education of China (20133702120002), and the Promotive Research Fund for Young and Middle-Aged Scientisits of Shandong Province, China (BS2013NY006). |
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Corresponding Authors:
KONG Ling-rang, Tel: +86-538-8249278, Fax: +86-538-8242226,E-mail: lkong@sdau.edu.cn
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| About author: MA Xin, E-mail: maxin138@163.com;* These authors contributed equally to this study. |
Cite this article:
MA Xin, DU Xu-ye, LIU Guo-juan, YANG Zai-dong, HOU Wen-qian, WANG Hong-wei, FENG De-shun.
2015.
Cloning and characterization of a novel UDP-glycosyltransferase gene induced by DON from wheat. Journal of Integrative Agriculture, 14(5): 830-838.
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| Anderson J, Stack R, Liu S, Waldron B. 2001. DNA markersfor Fusarium head blight resistance QTLs in two wheatpopulations. Theoretical and Applied Genetics, 102,1164-1168Anhalt S, Weissenböck G. 1992. Subcellular localization ofluteolin glucuronides and related enzymes in rye mesophyll.Planta, 187, 83-88Bai G H, Desjardins A E, Plattner R D. 2001. DeoxynivalenolnonproducingFusarium graminearum causes initialinfection, but does not cause disease spread in wheatspikes. Mycopathologia, 153, 91-98Bai G H, Shaner G. 1994. Scab of wheat: prospects for control.Plant Disease, 78, 760-776Basnet B R, Glover K D, Ibrahim A M H, Yen Y, Chao S M.2012. A QTL on chromosome 2DS of ‘Sumai 3’ increasessusceptibility to Fusarium head blight in wheat. Euphytica,186, 91-101Buerstmayr H, Ban T, Anderson J A. 2009. QTL mappingand marker-assisted selection for Fusarium head blightresistance in wheat: A review. Plant Breeding, 128, 1-26Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B,Stierschneider M, Ruckenbauer P. 2002. Molecularmapping of QTLs for Fusarium head blight resistancein spring wheat. I. Resistance to fungal spread (type IIresistance). Theoretical and Applied Genetics, 104, 84-91Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N,Lengauer D, Miedaner T, Schneider B, Lemmens M.2003. Molecular mapping of QTLs for Fusarium headblight resistance in spring wheat. II. Resistance to fungalpenetration and spread. Theoretical and Applied Genetics,107, 503-508Coutinho P M, Deleury E, Davies G J, Henrissat B. 2003.An evolving hierarchical family classification forglucosyltransferases. Journal of Molecular Biology, 328,307-317Fraissinet-Tachet L, Baltz R, Chong J, Kauffmann S,Fritig B, Saindrenan P. 1998. Two tobacco genesinduced by infection, elicitor and salicylic acid encodeglucosyltransferases acting on phenylpropanoids andbenzoic acid derivatives, including salicylic acid. FEBSLetters, 437, 319-323Goswami R S, Kistler H C. 2005. Pathogenicity and in plantamycotoxin accumulation among members of the Fusariumgraminearum species complex on wheat and rice.Phytopathology, 95, 1397-1404Handa H, Namiki N, Xu D H, Ban T. 2008. Dissecting of theFHB resistance QTL on the short arm of wheat chromosome2D using a comparative genomic approach: From QTL tocandidate gene. Molecular Breeding, 22, 71-84Horvath D, Huang D, Chua N H. 1998. Four classes ofsalicylate-induced tobacco genes. Molecular Plant-MicrobeInteractions, 11, 895-905Jansen C, von Wettstein D, Schafer W, Kogel K H, Felk A,Maier F J. 2005. Infection patterns in barley and wheatspikes inoculated with wild-type and trichodiene synthasegene disrupted Fusarium graminearum. Proceedings ofthe National Academy of Sciences of the United States ofAmerica, 102, 16892-16897Kang Z, Buchenauer H. 1999. Immunocytochemical localizationof fusarium toxins in infected wheat spikes by Fusariumculmorum. Physiological and Molecular Plant Pathology,55, 275-288Kong L R, Anderson J M, Ohm H W. 2005. Induction of wheatdefense and stress-related genes in response to Fusariumgraminearum. Genome, 48, 29-40Koornneef A, Pieterse C M J. 2008. Cross talk in defensesignaling. Plant Physiology, 46, 839-844Kunkel B N, Brooks D M. 2002. Cross talk between signalingpathways in pathogen defense. Current Opinion in PlantBiology, 5, 325-331Langevin F, Eudes F, Comeau A. 2004. Effect of trichothecenesproduced by Fusarium graminearum during Fusarium headblight development in six cereal species. European Journalof Plant Pathology, 110, 735-746Lemmens M, Scholz U, Berthiller F, Dall’Asta C, Koutnik A,Schuhmacher R, Adam G, Buerstmayr H, Mesterházy A, Krska R, Ruckenbauer P. 2005. The ability to detoxifythe mycotoxin deoxynivalenol colocalizes with a majorquantitative trait locus for Fusarium head blight resistancein wheat. Molecular Plant-Microbe Interactions, 18,1318-1324Li T, Bai G H, Wu S Y, Gu S L. 2011. Quantitative trait loci forresistance to Fusarium head blight in a Chinese wheatlandrace Haiyanzhong. Theoretical and Applied Genetics,122, 1497-1502Liu S, Zhang X, Pumphrey M O, Stack R W, Gill B S, AndersonJ A. 2006. Complex microcolinearity among wheat, rice,and barley revealed by fine mapping of the genomic regionharboring a major QTL for resistance to Fusarium headblight in wheat. Functional and Integrative Genomics, 6,83-89Ma L L, Shang Y, Cao A Z, Qi Z J, Xing L P, Chen P D, Liu D J,Wang X E. 2010. Molecular cloning and characterization ofan up-regulated UDP-glucosyltransferase gene induced byDON from Triticum aestivum L. cv. Wangshuibai. MolecularBiology Reports, 37, 785-795Makandar R, Nalam V J, Lee H, Trick H N, Dong Y H, Shah J.2012. Salicylic acid regulates basal resistance to Fusariumhead blight in wheat. Molecular Plant-Microbe Interactions,25, 431-439Malla S. 2012. Association of Fhb1 and Qfhs.ifa-5A in springversus winter growth habits in bread wheat (Triticumaestivum L.). Journal of Agricultural Science, 4, 39-48Mochida K, Yoshida T, Sakurai T, Ogihara Y, Shinozaki K.2009. TriFLDB: a database of clustered full-length codingsequences from Triticeae with applications to comparativegrass genomics. Plant Physiology, 150, 1135-1146Patricia A C, Daryl J S, Julian T, Sylvie C, Anita B B. 2006.Fine mapping Fhb1, a major gene controlling Fusariumhead blight resistance in bread wheat (Triticum aestivumL.). Theoretical and Applied Genetics, 112, 1465-1472Pestka J J, Zhou H R, Moon Y, Chung Y J. 2004. Cellularand molecular mechanisms for immune modulation bydeoxynivalenol and other trichothecenes: Unravelling aparadox. Toxicology Letters, 153, 61-73Poppenberger B, Berthiller F, Lucyshyn D, Sieberer T,Schuhmacher R, Krska R, Kuchler K, Glössl J, Luschnig C,Adam G. 2003. Detoxification of the Fusarium graminearummycotoxin deoxynivalenol by a UDP-glucosyltransferasefrom Arabidopsis thaliana. The Journal of BiologicalChemistry, 278, 47905-47914Ren L J, Shen X R, Zhou M P, Zhang X, Ma H X, Lu W Z,Nichoson P. 2003. SSR markers for Fusarium head blightresistance QTLs in three wheat populations. AgriculturalSciences in China, 2, 987-993Rocha O, Ansari K, Doohan F M. 2005. Effects of trichothecenemycotoxins on eukaryotic cells: A review. Food Additivesand Contaminants, 22, 369-378Ross J, Li Y, Lim E K, Bowles D J. 2001. Higher plantglycosyltransferases. Genome Biology, 2, 3004.1-30046.Saghai-Maroof M A, Soliman K M, Jorgensen R A, Allard RW. 1984. Ribosomal DNA space length polymorphisms inbarley: Mendelian inheritance, chromosomal locations andpopulation dynamics. Proceedings of the National Academyof Sciences of the United States of America, 81, 8014-8018Schweiger W, Boddu J, Shin S, Poppenberger B, BerthillerF, Lemmens M, Muehlbauer G J, Adam G. 2010.Validation of a candidate deoxynivalenol-inactivating UDPglucosyltransferasefrom barley by heterologous expressionin yeast. Molecular Plant-Microbe Interactions, 23, 977-986Schweiger W, Pasquet J C, Nussbaumer T, Paris M P K,Wiesenberger G, Macadré C, Ametz C, Berthiller F,Lemmens M, Saindrenan P, Mewes H W, Mayer K F X,Dufresne M, Adam G. 2013a. Functional characterizationof two clusters of Brachypodium distachyon UDPglycosyltransferasesencoding putative deoxynivalenoldetoxification genes. Molecular Plant-Microbe Interactions,26, 781-792Schweiger W, Steiner B, Ametz C, Siegwart G, WiesenbergerG, Berthiller F, Lemmens M, Jia H Y, Adam G, MuehlbauerG J, Kreil D P, Buerstmayr H. 2013b. Transcriptomiccharacterization of two major Fusarium resistancequantitative trait loci (QTLs), Fhb1 and Qfhs.ifa-5A,identifies novel candidate genes. Molecular PlantPathology, 14, 772-785Schweiger W, Steiner B, Limmongkon A, Brunner K. 2011.Cloning and heterologous expression of candidate DONinactivatingUDP-glucosyltransferases from rice and wheatin yeast. Plant Breeding and Seed Science, 64, 105-111Shin S, Torres-Acosta J A, Heinen S J, McCormick S, LemmensM, Paris M P, Berthiller F, Adam G, Muehlbauer G J. 2012.Transgenic Arabidopsis thaliana expressing a barley UDPglucosyltransferaseexhibit resistance to the mycotoxindeoxynivalenol. Journal of Experimental Botany, 63,4731-4740Teardo E, Formentin E, Segalla A, Giacometti G M, Marin O,Zanetti M, Schiavo F L, Zoratti M, Szabò I. 2011. Duallocalization of plant glutamate receptor AtGLR3.4 to plastidsand plasmamembrane. Biochimica et Biophysica Acta,1807, 359-367Wilde F, Miedaner T. 2006. Selection for Fusarium head blightresistance in early generations reduces the deoxynivalenol(DON) content in grain of winter and spring wheat. PlantBreeding, 125, 96-98Xu D H, Juan H F, Nohda M, Ban T. 2001. QTL mapping oftype I and type II resistance to FHB in wheat. In: NationalFusarium head blight Forum Proceedings, Erlanger, KY,USA. pp. 40-42Yazaki K, Inushima K, Kataoka M, Tabata M. 1995.Intracellular localization of UDPG: p-hydroxybenzoateglucosyltransferase and its reaction product in lithospermumcell cultures. Phytochemistry, 38, 1127-1130 |
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