|
|
|
|
|
| Magnaporthe Rab5 homologs show distinct functions in nerve growth factor (NGF)-mediated neurite outgrowth and cell differentiation |
| QI Yao-yao, LIANG Zhi-min, ZHOU Jie, WANG Zong-hua, LU Guo-dong, LI Guang-pu |
1、Key Laboratory of Biopesticides and Chemical Biology, Ministry of Education/Fujian Agriculture and Forestry University, Fuzhou
350002, P.R.China
2、Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma 73104, USA |
|
|
|
摘要 Nerve growth factor (NGF) binds to TrkA and forms a NGF/TrkA complex at the cell surface, which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth. The small GTPase Rab5 is reported to localize on the plasma membrane and early endosomes, regulating endosome fusion. It was reported that endogenous Rab5 function may need to be suppressed during NGF-induced neurite outgrowth and cell differentiation. Two Rab5 homologs (MoRab5A: MGG_06241 and MoRab5B:MGG_01185) were characterized from the rice blast fungus Magnaporthe oryzae, and MoRab5B was identified as the Rab5 ortholog promoting early endosomal fusion, while MoRab5A specialized to perform a non-redundant function in endosomal sorting. In this study, we examined whether MoRab5A and MoRab5B play different roles in NGF-induced neurite outgrowth and cell differentiation in PC12 cells (a rat pheochromocytoma cell line). Our data showed that MoRab5B is a negative regulator of NGF signaling and neurite outgrowth in PC12 cells, similar to human Rab5 (hRab5). MoRab5B:WT inhibits NGF signaling-dependent neurite outgrowth while the dominant-negative MoRab5B mutant (MoRab5B:DN) enhances NGF signaling and neurite outgrowth. In contrast, MoRab5A:WT and MoRab5A:DN both significantly promote NGF-induced neurite outgrowth, indicating that MoRab5B is more similar to hRab5 than MoRab5A in the regulation of NGF signal transduction.
Abstract Nerve growth factor (NGF) binds to TrkA and forms a NGF/TrkA complex at the cell surface, which is then internalized into signaling endosomes and promotes neuronal survival and neurite outgrowth. The small GTPase Rab5 is reported to localize on the plasma membrane and early endosomes, regulating endosome fusion. It was reported that endogenous Rab5 function may need to be suppressed during NGF-induced neurite outgrowth and cell differentiation. Two Rab5 homologs (MoRab5A: MGG_06241 and MoRab5B:MGG_01185) were characterized from the rice blast fungus Magnaporthe oryzae, and MoRab5B was identified as the Rab5 ortholog promoting early endosomal fusion, while MoRab5A specialized to perform a non-redundant function in endosomal sorting. In this study, we examined whether MoRab5A and MoRab5B play different roles in NGF-induced neurite outgrowth and cell differentiation in PC12 cells (a rat pheochromocytoma cell line). Our data showed that MoRab5B is a negative regulator of NGF signaling and neurite outgrowth in PC12 cells, similar to human Rab5 (hRab5). MoRab5B:WT inhibits NGF signaling-dependent neurite outgrowth while the dominant-negative MoRab5B mutant (MoRab5B:DN) enhances NGF signaling and neurite outgrowth. In contrast, MoRab5A:WT and MoRab5A:DN both significantly promote NGF-induced neurite outgrowth, indicating that MoRab5B is more similar to hRab5 than MoRab5A in the regulation of NGF signal transduction.
|
|
Received: 18 December 2014
Accepted:
|
| Fund: This work was supported, in whole or in part, by the National Institutes of Health Grant, USA (R01GM074692), the National Nature Sciences Foundation of China (31328002 and 31070124) and a scholarship from the China Scholarship Council. |
|
Corresponding Authors:
LU Guo-dong, Tel: +86-591-83789478,E-mail: gdlufafu@163.com; LI Guang-pu, Tel: +86-405-2712227,Fax: +86-405-2713910, E-mail: guangpu-li@ouhsc.edu
E-mail: gdlufafu@163.com;guangpu-li@ouhsc.edu
|
| About author: Received 18 December, 2014 Accepted 4 March, 2015 QI Yao-yao, Mobile: 15980545390, E-mail: wenxuerubing@sohu.com; |
Cite this article:
QI Yao-yao, LIANG Zhi-min, ZHOU Jie, WANG Zong-hua, LU Guo-dong, LI Guang-pu.
2015.
Magnaporthe Rab5 homologs show distinct functions in nerve growth factor (NGF)-mediated neurite outgrowth and cell differentiation. Journal of Integrative Agriculture, 14(5): 823-829.
|
| Ao X, Zou L, Wu Y. 2014. Regulation of autophagy by theRab GTPase network. Cell Death and Differentiation, 21,348-358Chavrier P, Parton R G, Hauri H P, Simons K, Zerial M. 1990.Localization of low molecular weight GTP binding proteinsto exocytic and endocytic compartments. Cell, 62, 317-329Ebbole D J. 2007. Magnaporthe as a model for understanding host-pathogen interactions. Annual Review ofPhytopathology, 45, 437-456Greene L A, Shooter E M. 1980. The nerve growth factor:biochemistry, synthesis, and mechanism of action. AnnualReview of Neuroscience, 3, 353-402Gorvel J P, Chavrier P, Zerial M, Gruenberg J. 1991. Rab5controls early endosome fusion in vitro. Cell, 64, 915-925Li G, Stahl P D. 1993. Post-translational processing andmembrane association of the two early endosomeassociatedrab GTP-binding proteins (rab4 and rab5).Archives of Biochemistry and Biophysics, 304, 471-478Li G, Segev N, 2012. Ypt/Rab GTPases and intracellularmembrane trafficking: An overview. Rab GTPases andMembrane Trafficking. Bentham Science Publishers, USA.pp. 3-17Liu J, Lamb D, Chou M M, Liu Y J, Li G. 2007. Nerve growthfactor-mediated neurite outgrowth via regulation of Rab5.Molecular Biology of the Cell, 18, 1375-1384Manna D, Aligo J, Xu C, Park W S, Koc H, Heo W D, Konan KV. 2010. Endocytic Rab proteins are required for hepatitisC virus replication complex formation. Virology, 398, 21-37Qi Y, Marlin M C, Liang Z, Berry W L, Janknecht R, ZhouJ, Wang Z, Lu G, Li G. 2014. Distinct biochemical andfunctional properties of two Rab5 homologs from the riceblast fungus Magnaporthe oryzae. The Journal of BiologicalChemistry, 289, 28299-28309Raiborg C, Stenmark H. 2009. The ESCRT machinery inendosomal sorting of ubiquitylated membrane proteins.Nature, 458, 445-452Saxena S, Bucci C, Weis J, Kruttgen A. 2005. The small GTPaseRab7 controls the endosomal trafficking and neuritogenicsignaling of the nerve growth factor receptor TrkA. TheJournal of Neuroscience: the Official Journal of the Societyfor Neuroscience, 25, 10930-10940Serio G, Margaria V, Jensen S, Oldani A, Bartek J, BussolinoF, Lanzetti L. 2011. Small GTPase Rab5 participates inchromosome congression and regulates localization of thecentromere-associated protein CENP-F to kinetochores.Proceedings of the National Academy of Sciences of theUnited States of America, 108, 17337-17342Talbot N J. 2003. On the trail of a cereal killer: Exploringthe biology of Magnaporthe grisea. Annual Review ofMicrobiology, 57, 177-202Valent B, Chumley F G. 1991. Molecular genetic analysis ofthe rice blast fungus, Magnaporthe grisea. Annual Reviewof Phytopathology, 29, 443-467Wang L, Liang Z, Li G. 2011. Rab22 controls NGF signalingand neurite outgrowth in PC12 cells. Molecular Biology ofthe Cell, 22, 3853–3860. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
