Please wait a minute...
Journal of Integrative Agriculture  2015, Vol. 14 Issue (3): 497-502    DOI: 10.1016/S2095-3119(14)60826-1
Special Focus: Direct-Fed Microbial - Animal Nutrition and Reproduction Responses Advanced Online Publication | Current Issue | Archive | Adv Search |
Effect of a bacterial inoculum and additive on dry matter in situ degradability of sugarcane silage
 José A Reyes-Gutiérrez, Oziel D Montañez-Valdez, Ramón Rodríguez-Macias, Mario Ruíz-López, Eduardo Salcedo-Pérez, Cándido E Guerra-Medina
1、South University Center, University of Guadalajara, Ciudad Guzmán 49000, Mexico
2、University Center for Biological and Agricultural Science, University of Guadalajara, Las Agujas, Zapopan 45110, Mexico
3、South Coast University Center, University of Guadalajara, Autlán de Navarro 48900, Mexico
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  The objective of this study was to evaluate the effect of adding a bacterial inoculum and a handmade additive to sugarcane silage (SCS) on the in situ digestibility of dry matter (DM). The treatments were: T1) sugarcane silage (SCS) and T2) sugarcane silage with 1% inoculum and 1% additive (SCS+). The bacterial inoculum consisted of 10.0% molasses, 1.0% yogurt, 5.0% chicken manure, 0.5% urea, and 83.0% water, and the additive was formulated with 1.0% urea, 0.1% ammonium sulfate, and 0.25% phosphorus. In situ dry matter digestibility (DMD) was determined using the nylon bag technique with four cows equipped with ruminal fistulas. Cows were fed with ensiled sugarcane supplemented with 1 kg of commercial concentrate. 5 g of ground sample for each sugarcane treatment were weighted in nylon bags and incubated for 4, 8, 12, 24, 48, and 72 h in a completely randomized design with six replicates. The DMD (%) was higher (P<0.05) for SCS+ for all incubation times when compared with SCS. There were no differences in ruminal pH between the treatments for all the incubation times. The data suggested that the sugarcane silage with bacterial inoculum and additive could be an alternative for providing forage for ruminants during the season of low growth and quality grass.

Abstract  The objective of this study was to evaluate the effect of adding a bacterial inoculum and a handmade additive to sugarcane silage (SCS) on the in situ digestibility of dry matter (DM). The treatments were: T1) sugarcane silage (SCS) and T2) sugarcane silage with 1% inoculum and 1% additive (SCS+). The bacterial inoculum consisted of 10.0% molasses, 1.0% yogurt, 5.0% chicken manure, 0.5% urea, and 83.0% water, and the additive was formulated with 1.0% urea, 0.1% ammonium sulfate, and 0.25% phosphorus. In situ dry matter digestibility (DMD) was determined using the nylon bag technique with four cows equipped with ruminal fistulas. Cows were fed with ensiled sugarcane supplemented with 1 kg of commercial concentrate. 5 g of ground sample for each sugarcane treatment were weighted in nylon bags and incubated for 4, 8, 12, 24, 48, and 72 h in a completely randomized design with six replicates. The DMD (%) was higher (P<0.05) for SCS+ for all incubation times when compared with SCS. There were no differences in ruminal pH between the treatments for all the incubation times. The data suggested that the sugarcane silage with bacterial inoculum and additive could be an alternative for providing forage for ruminants during the season of low growth and quality grass.
Keywords:  sugarcane silage       degradability       bacterial       cows  
Received: 12 December 2013   Accepted:
Fund: 

the National Council for Science and Technology, Mexico (CONACyT) for scholarship support.

Corresponding Authors:  Oziel D Monta?ez-Valdez, Tel: +52-341-5752222-46115, E-mail: montanez77@hotmail.com     E-mail:  montanez77@hotmail.com

Cite this article: 

José A Reyes-Gutiérrez, Oziel D Monta?ez-Valdez, Ramón Rodríguez-Macias, Mario Ruíz-López, Eduardo Salcedo-Pérez, Cándido E Guerra-Medina. 2015. Effect of a bacterial inoculum and additive on dry matter in situ degradability of sugarcane silage. Journal of Integrative Agriculture, 14(3): 497-502.

Abdel-Aziz N A, El-Adawy M M, Salem A Z M, Cerrillo-Soto MA, Camacho L M, Borhami B E. 2014. Effects of exogenousenzymes, Lactobacillus acidophilus or their combinationon feed intake, digestibility and performance of rabbits fedsugarcane bagasse. Animal Nutrition and Feed Technology,14, 137-145

Abdel-Aziz N A, El-Adawy M, Mariezcurrena-Berasain M A,Salem A Z M, Olivares-Pérez J, Kholif A E, Borhami BE. 2015a. Effects of exogenous enzymes, Lactobacillusacidophilus or their combination on feed performanceresponse and carcass characteristics of rabbits fedsugarcane bagasse. Journal of Integrative Agriculture, 14,544-549

Abdel-Aziz N A, Salem A Z M, El-Adawy M M, Camacho LM, Kholif A E, Elghandour M M Y, Borhami B E. 2015b.Biological treatments as a mean to improve feed utilizationin agriculture animals-An overview. Journal of IntegrativeAgriculture, 14, 534-543

Abo-Donia F M, Abdel-Azim S N, Elghandour M M Y, SalemA Z M, Buendia G, Soliman N A M. 2014. Feed intake,nutrient digestibility and ruminal fermentation activities insheep fed peanut hulls treated with Trichoderma viride orurea. Tropical Animal Health and Production, 46, 221-228

Alli I, Baker B E. 1982. Studies on the fermentation of choppedsugarcane. Animal Feed Science and Technology, 7,411-417

Alli I, Fairbairn R, Baker B E, Garcia G. 1983. The effectsof ammonia on the fermentation of chopped sugarcane.Animal Feed Science and Technology, 9, 291-299

AOAC (Association of Official Analytical Chemists). 2007.Official Methods of Analysis. 18th ed. Association of OfficialAnalytical Chemists, Washington, D.C.

Aranda E M, Mendoza G D, Ramos J A, da Silva I C, Vitti AC. 2010. Effect of fibrolitic enzymes on rumen microbialdegradation of sugarcane fiber. Ciencia Animal Brasileira,11, 448-495(in Portuguese)

Aranda E M, Ruiz P, Mendoza G D, Marcoff C F, Ramos J A,Elías A. 2004. Changes in the digestion of three varietiesof sugarcane and their fiber fractions. Cuban Journal ofAgricultural Science, 38, 135-141

Avila C L S, Bravo M C E C, Schwan R F. 2010. Identificationand characterization of yeasts in sugarcane silages. Journalof Applied Microbiology, 109, 1677-1686

Avila C L S, Pinto J C, Carvalho B F. 2013. Use of additives inensiling of sugar cane. Informe Agropecuario, 34, 65-72(in Portuguese)

Avila C L S, Pinto J C, Figueiredo H C P, Shwan R F. 2009.Effects of an indigenous and a commercial Lactobacillusbuchneri strain on quality of sugar cane silage. Grass andForage Science, 64, 384-394

Bergamaschine A F, Passipiéri M, Veriano F W V, IseponO J, de Almeida C L. 2006. Quality and nutritive valueof marandugrass (Brachiaria brizanthia, cv. Marandu)silage prepared with additive or wilting. Revista Brasileirade Zootecnia (Brazilian Journal of Animal Science), 35,1454-1462

Bolsen K.1995. Silage: Basic principles. In: Barnes R F, MillerD A, Nelson C J, eds., Forages. Iowa State University,UAS. pp. 163-176

Carvalho B F, Avila C L S, Pinto J C, Schwan R. F. 2013. Effectof propionic acid and Lactobacillus plantarum UFLA SIL 1on the sugarcane silage with and without calcium oxide.African Journal of Microbiology Research, 7, 4159-4168

Daniel J L P, Weiß K, Custódio L, Neto A S, Santos M C,Zopollatto M, Nussio L G. 2013. Occurrence of volatileorganic compounds in sugarcane silages. Animal FeedScience and Technology, 185, 101-105

Elghandour M M Y, Salem A Z M, Martínez Castañeda J S,Camacho L M, Kholif A E, Vázquez Chagoyán J C. 2015.Direct-fed microbes: A tool for improving the utilization oflow quality roughages in ruminants. Journal of IntegrativeAgriculture, 14, 526-533

Espinoza F, Argenti P, Carrillo C, Araque C, Torres A, Valle A.2006. Strategic use of sugar cane (Saccharum officinarum)in dual purpose pregnant heifers. Zootecnia Tropical, 24,95-107 (in Spanish)

Ferreira D A, Gonçalves L, Molina L R, Castro-Neto A, TomichT R. 2007. Fermentation of sugarcane silage treated withurea, zeolita, bacteria inoculant and bacteria/enzymatic inoculants. Arquivo Brasileira de Medicina VeterináriaZootecnia, 59, 423-433

García H, Abreu M, Soto J M. 2008. Digestion of residualsof the crop cane treatment with OHNa. 1. Determinationof digestibility in situ. REDVET (Electronic Journal ofVeterinary Medicine), 11, 1-8 (in Spainish)

Khattab I M, Salem A Z M, Abdel-Wahed A M, Kewan K Z. 2013.Effects of urea supplementation on nutrient digestibility,nitrogen utilization and rumen fermentation in sheep feddiets containing dates. Livestock Science, 155, 223-229

Kung Jr L, Stanley R W. 1982. Effect of stage of maturity onthe nutritive value of whole-plant sugarcane preserved assilage. Journal of Animal Science, 54, 689-696

Liu J J, Liu X P, Ren J W, Zhao H Y, Yuan X F, Wang X F,Salem A Z M, Cui Z J. 2015. The effects of fermentationand adsorption using lactic acid bacteria culture broth on thefeed quality of rice straw. Journal of Integrative Agriculture,14, 503-513

McCullough H. 1967. The determination of ammonia in wholeblood by a direct colorimetric method. Clinica Chimica Acta,17, 297-304

McDonald I. 1981. A revised model for estimation of proteindegradability in the rumen. Journal of Agricultural Science,96, 251-252

Molina A S, Sierra J F, Febles I. 1999. Sugar cane silage withprotein synthesis: Combined effect of additives and density.Cuban Journal of Agricultural Science, 33, 205-208

Muck R E. 2010. Silage microbiology and its control throughadditives. Revista Brasileira de Zootecnia (Brazilian Journalof Animal Science), 39, 183-191

Ørskov E R, McDonald I. 1979. The estimation of proteindegradability in the rumen from incubation measurementsweighted according to rate of passage. Journal ofAgricultural Science, 92, 499-503

Pedroso A F, Andrade R A, Barioni B J, Batista S G.2011. Fermentation parameters, quality and losses insugarcane silages treated with chemical additives anda bacterial inoculant. Revista Brasileira de Zootecnia(Brazilian Journal of Animal Science), 40, 2318-2322

Pedroso A F. 2003. Chemical additive and microbial inoculantseffects on the fermentation and on the control of the alcoholproduction in sugarcane silages. Ph D thesis, University ofSão Paulo, Piracicaba, Brasil. p. 120.

Pedroso A F, Nussio L, Lourdes D R. 2007. Effect of treatmentwith chemical additives and bacterial losses and quality ofsilage from sugar cane. Revista Brasileira de Zootecnia(Brazilian Journal of Animal Science), 36, 558-564

Pedroso A F, Nussio L, Paziani S F. 2005. Fermentation andepiphytic microflora dynamics in sugar cane silage. ScientiaAgricola, 62, 427-432

Rezende S G, Andrade R R, Schoken R P I, Fernandes B T,Vieira P A J, Toledo P R M, Toledo P R M. 2007. Chemicaland bacterial addition association on the sugarcaneensilage. Revista Brasileira de Zootecnia (Brazilian Journalof Animal Science), 36, 789-798

Rezende S G, Andrade R R, Schocken-Iturrino R P, VieiraP A J, Fernandes B T, Toledo P R M. 2010. Burning andchemical and bacterial additives in sugar cane silage.Revista Brasileira de Zootecnia (Brazilian Journal of AnimalScience), 39, 103-112(in Portuguese)

Rocha K D, Pereira O G, Valadares F S C, Oliveira A P, PachecoL B B, Chizzotti F H M. 2006. Nutritive value of corn silage(Zea mays L.) produced with enzymatic-bacterial inoculants.Revista Brasileira de Zootecnia (Brazilian Journal of AnimalScience), 35, 389-395 (in Portuguese)

Rocha V A, Cardoso P J, Da Silva A C, Ricardo E A, Botego TV, Freitas S R. 2009. Effect of the addition of Lactobacillussp. in sugarcane silages. Revista Brasileira de Zootecnia(Brazilian Journal of Animal Science), 38,1009-1017 (inPortuguese)

Rötz C A, Muck R E. 1994. Changes in forage quality duringharvest and storage. In: Fahey G C J, Collins M, MertensD R, Moser L E, eds., Forage Quality, Evaluation andUtilization. Madison, WI. pp. 828-868

Ruiz B O, Castillo Y, Anchondo A, Rodríguez C, Beltrán R, LaO O, Payán J. 2009. Enzyme and inoculant effects on cornsilage composition. Archivos de Zootecnia, 58, 163-172(in Spanish)

Russell B J, Wilson B D. 1996. Why are ruminal cellulolyticbacteria unable to digest cellulose at low pH? Journal ofDairy Science, 79, 1503-1509

Salem A Z M, Zhou C S, Tan Z L, Mellado M, Odongo N E,Cipriano-Salazar M, Elghandour M M Y. 2013. In vitrodigestibility, chemical composition, and kinetics of gasproduction of foliage from four fodder trees ensiled withor without molasses and urea. Journal of IntegrativeAgriculture, 12, 1234-1242

SáNeto A N, Nussio L G, Zopollatto M, Junges D, Wosniak AB. 2013. Corn and sugarcane silages with Lactobacillusbuchneri alone or associated with L. plantarum. PesquisaAgropecuaria Brasileira, 48, 528-535 (in Portuguese)

Sampaio I B M. 1988. Experimental designs and modellingtechniques in the study of roughage degradation in rumenand growth of ruminants. Ph D thesis, University of Reading,United Kingdom. p. 214.

Santos M C, Nussio L G, Mourão G B. 2009. Nutritive value ofsugarcane silage treated with chemical additives. ScientiaAgricola, 66, 159-163

SAS. 1999. User’s Guide: Statistics. 8th ed. SAS Institute,Cary NC.Van Soest P J, Robertson J B, Lewis B A. 1991. Methodsfor dietary fiber, neutral detergent fiber and non-starchpolysaccharides in relation to animal nutrition. Journal ofDairy Science, 74, 3583-3597

Tejada de Hernández I. 1985. Laboratory Manual for Analysisof Ingredients Used in Animal Feeding. Board SupportResearch and Experimentation in Mexico Cattle, Mexico.p. 387. (in Spanish)Vanzant E S, Cochran R C, Titgemeyer E C. 1998.Standardization of in situ techniques for ruminant feedstuffevaluation. Journal of Animal Science, 76, 2717-2729
[1] Chaoyue Pang, Ling Jin, Haoyu Zang, Damalk Saint-Claire S. Koklannou, Jiazhi Sun, Jiawei Yang, Yongxing Wang, Liang Xu, Chunyan Gu, Yang Sun, Xing Chen, Yu Chen. Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR[J]. >Journal of Integrative Agriculture, 2024, 23(5): 1580-1592.
[2] Qianqian Chen, Qian Zhao, Baoxing Xie, Xing Lu, Qi Guo, Guoxuan Liu, Ming Zhou, Jihui Tian, Weiguo Lu, Kang Chen, Jiang Tian, Cuiyue Liang.

Soybean (Glycine max) rhizosphere organic phosphorus recycling relies on acid phosphatase activity and specific phosphorus-mineralizing-related bacteria in phosphate deficient acidic soils [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1685-1702.

[3] Shanshan Su, Hongwu Liu, Junrong Zhang, Puying Qi, Yue Ding, Ling Zhang, Linli Yang, Liwei Liu, Xiang Zhou, Song Yang.

Discovery and structure-activity relationship studies of novel tetrahydro-β-carboline derivatives as apoptosis initiators for treating bacterial infections [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1259-1273.

[4] Minghui Cao, Yan Duan, Minghao Li, Caiguo Tang, Wenjie Kan, Jiangye Li, Huilan Zhang, Wenling Zhong, Lifang Wu.

Manure substitution improves maize yield by promoting soil fertility and mediating the microbial community in lime concretion black soil [J]. >Journal of Integrative Agriculture, 2024, 23(2): 698-710.

[5] Jia Fu, Jie Fan, Chenxi Zhang, Yongyao Fu, Baohang Xian, Qiyuan Yu, Xin Huang, Wen Yang, Shanchun Chen, Yongrui He, Qiang Li.

High-throughput screening system of citrus bacterial canker-associated transcription factors and its application to the regulation of citrus canker resistance [J]. >Journal of Integrative Agriculture, 2024, 23(1): 155-165.

[6] CHEN Xiao-qiang, LIU Jia-yan, HUANG Xue-jun, WEI Yan-an, SHAO Rui-xiang, CHEN Ting-ting, XIE Jian-chun . Physicochemical properties and antibacterial mechanism of theabrownins prepared from different catechins catalyzed by polyphenol oxidase and peroxidase[J]. >Journal of Integrative Agriculture, 2023, 22(9): 2905-2916.
[7] SONG Ying-lian, LIU Hong-wu, YANG Yi-hong, HE Jing-jing, YANG Bin-xin, YANG Lin-li, ZHOU Xiang, LIU Li-wei, WANG Pei-yi, YANG Song. Novel 18β-glycyrrhetinic acid amide derivatives show dual-acting capabilities for controlling plant bacterial diseases through ROS-mediated antibacterial efficiency and activating plant defense responses[J]. >Journal of Integrative Agriculture, 2023, 22(9): 2759-2771.
[8] WANG Yuan, ZHOU Hong-zhang, GAO Yu, WANG Ning-wei, LIU Han, YANG Fu-yu, NI Kui-kui. Ensiling vine tea (Ampelopsis grossedentata) residue with Lactobacillus plantarum inoculant as an animal unconventional fodder[J]. >Journal of Integrative Agriculture, 2023, 22(4): 1172-1183.
[9] CAO Peng, CHEN Jia-lan, LI Ning-ning, ZHANG Shuang-xi, WANG Rong-bo, LI Ben-jin, LIU Pei-qing, AN Yu-yan, ZHANG Mei-xiang. Seedling Petri-dish inoculation method: A robust, easy-to-use and reliable assay for studying plant–Ralstonia solanacearum interactions[J]. >Journal of Integrative Agriculture, 2023, 22(12): 3709-3719.
[10] ZOU Xiao-xia, HUANG Ming-ming, LIU Yan, SI Tong, ZHANG Xiao-jun, YU Xiao-na, GUO Feng, WAN Shu-bo. Inclusion of peanut in wheat–maize rotation increases wheat yield and net return and improves soil organic carbon pool by optimizing bacterial community[J]. >Journal of Integrative Agriculture, 2023, 22(11): 3430-3443.
[11] Wannaporn THEPBANDIT, Narendra Kumar PAPATHOTI, Jayasimha Rayulu DADDAM, Nguyen Huy HOANG, Toan LE THANH, Chanon SAENGCHAN, Kumrai BUENSANTEAI. In vitro and in silico studies of salicylic acid on systemic induced resistance against bacterial leaf blight disease and enhancement of crop yield[J]. >Journal of Integrative Agriculture, 2023, 22(1): 170-184.
[12] SONG Ya-na, CHEN Zai-jie, WU Ming-ji, LI Gang, WANG Feng. Changes in bacterial community and abundance of functional genes in paddy soil with cry1Ab transgenic rice[J]. >Journal of Integrative Agriculture, 2021, 20(6): 1674-1686.
[13] CUI Hu, OU Yang, WANG Li-xia, YAN Bai-xing, LI Ying-xin, DING Da-wei. Phosphate rock reduces the bioavailability of heavy metals by influencing the bacterial communities during aerobic composting[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1137-1146.
[14] XIN Yuan-yuan, Anisur RAHMAN, LI Hui-xiu, XU Ting, DING Guo-chun, LI Ji. Modification of total and phosphorus mineralizing bacterial communities associated with Zea mays L. through plant development and fertilization regimes[J]. >Journal of Integrative Agriculture, 2021, 20(11): 3026-3038.
[15] Cyrine REZGUI, Isabelle TRINSOUTROT-GATTIN, Marie BENOIT, Karine LAVAL, Wassila RIAH-ANGLET. Linking changes in the soil microbial community to C and N dynamics during crop residue decomposition[J]. >Journal of Integrative Agriculture, 2021, 20(11): 3039-3059.
No Suggested Reading articles found!