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| Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens |
| GUO Dong-chun, SUN Yan, ZHANG Ai-qin, LIU Jia-sen, LU Yan, LIU Pei-xin, YUAN Dongwei, JIANG Qian, SI Chang-de , QU Lian-dong |
1、State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin
150001, P.R.China
2、College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 159001, P.R.China |
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摘要 Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin genes, fhaB1 and fhaB2, are the potential virulence factors. In this study, an inactivation fhaB1 mutant of P. multocida in avian strain C48-102 was constructed by a kanamycin-resistance cassette. The virulence of the fhaB1 mutant and the wild type strain was assessed in chickens by intranasal and intramuscular challenge. The inactivation of fhaB1 resulted in a high degree of attenuation when the chickens were challenged intranasally and a lesser degree when challenged intramuscularly. The fhaB1 mutant and the wild type strain were investigated their sensitivity to the antibody-dependent classical complement-mediated killing pathway in 90% convalescent chicken serum. The fhaB1 mutant was serum sensitive as the viability has reduced between untreated serum and heat inactivated chicken serum (P<0.007). These results confirmed that FhaB1 played the critical roles in the bacterial pathogenesis and further studies were needed to investigate the mechanism which caused reduced virulence of the fhaB1 mutant.
Abstract Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin genes, fhaB1 and fhaB2, are the potential virulence factors. In this study, an inactivation fhaB1 mutant of P. multocida in avian strain C48-102 was constructed by a kanamycin-resistance cassette. The virulence of the fhaB1 mutant and the wild type strain was assessed in chickens by intranasal and intramuscular challenge. The inactivation of fhaB1 resulted in a high degree of attenuation when the chickens were challenged intranasally and a lesser degree when challenged intramuscularly. The fhaB1 mutant and the wild type strain were investigated their sensitivity to the antibody-dependent classical complement-mediated killing pathway in 90% convalescent chicken serum. The fhaB1 mutant was serum sensitive as the viability has reduced between untreated serum and heat inactivated chicken serum (P<0.007). These results confirmed that FhaB1 played the critical roles in the bacterial pathogenesis and further studies were needed to investigate the mechanism which caused reduced virulence of the fhaB1 mutant.
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Received: 06 August 2013
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31302109). |
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Corresponding Authors:
QU Lian-dong, Tel: +86-451-51997165, E-mail: qld@hvri.ac.cn
E-mail: qld@hvri.ac.cn
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| About author: GUO Dong-chun, E-mail: gdongchun@126.com |
Cite this article:
GUO Dong-chun, SUN Yan, ZHANG Ai-qin, LIU Jia-sen, LU Yan, LIU Pei-xin, YUAN Dongwei, JIANG Qian, SI Chang-de , QU Lian-dong.
2014.
Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens. Journal of Integrative Agriculture, 13(10): 2268-2275.
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| Berggard K, Johnsson E, Mooi F R, Lindahl G. 1997.Bordetella pertussis binds the human complementC4BP: Role of filamentous hemagglutinin. Infectionand Immunity, 65, 638-643Boyce J D, Adler B. 2000. The capsule is a virulencedeterminant in the pathogenesis of Pasteurella multocidaM1404 (B:2). Infection and Immunity, 68, 3463-3468Carter G R. 1955. Studies on Pasteurella multocida I: Ahaemagglutination test for the identification of serologicaltypes. America Journal of Veterinary Research, 16, 481- 484.Chung J Y, Wilkie I, Boyce J D, Adler B. 2005. Vaccinationagainst fowl cholera with acapsular Pasteurella multocidaA:1. Vaccine, 23, 2751-2755Chung J Y, Wilkie I, Boyce J D, Townsend K M, Frost AJ, Ghoddusi M, Adler B. 2001. Role of capsule in thepathogenesis of fowl cholera caused by Pasteurellamultocida serogroup A. Infection and Immunity, 69,2487-2492Cole S P, Guiney D G, Corbeil L B. 1993. Molecular analysisof a gene encoding a serum-resistance-associated 76 kDasurface antigen of Haemophilus somnus. Journal GeneralMicrobiology, 9, 2135-2143Dabo S M, Confer A W, Hartson S D. 2005. Adherenceof Pasteurella multocida to fibronectin. VeterinaryMicrobiology, 110, 265-275Dabo S M, Confer A W, Quijano-Blas R A. 2003. Molecularand immunological characterization of Pasteurellamultocida serotype A:3 OmpA: Evidence of its role in P.multocida interaction with extracellular matrix molecules.Microbial Pathogenesis, 35, 147-157Fernandez de Henswtrosa A R, Badiola I, Caco M, Perez deRozas A M, Campoy S, Barbe J. 1997. Importance of thegalE gene on the virulence of Pasteurella multocida. FEMSMicrobiology Letter, 154, 311-316Fuller T E, Kennedy M J, Lowery D E. 2000. Identificationof Pasteurella multocida virulence genes in a septicemiamouse model using signature-tagged mutagenesis.Microbial Pathogenesis, 29, 25-38Guo D C, Lu Y, Zhang A Q, Liu J S, Yuan D W, Jiang Q, Lin H,Si C D, Qu L D. 2012. Identification of genes transcribed byPasteurella multocida in rabbit livers through the selectivecapture of transcribed sequences. FEMS MicrobiologyLetters, 331, 105-112Harper M, Cox A D, St Michael F, Wilkie I W, Boyce J D,Adler B. 2004. A heptosyltransferase mutant of Pasteurellamultocida produces a truncated lipopolysaccharidestructure and is attenuated in virulence. Infectionand Immunity, 72, 3436-3443Harper M, Cox A D, Adler B, Boyce J D. 2011. Pasteurellamultocida lipopolysaccharide structures: The long and theshort of it. Veterinary Microbiology, 153, 109-115Harper M, Cox A D, St Michael F, Ford M, Wilkie I W,Boyce J D, Adler B. 2010. Natural selection in the chickenhost inditifies 3-deoxy-D-manno-octuosonic acid kinaseresidues essential for phosphorylation of Pasteurellamultocida lipopolysaccharide. Infection and Immunity,78, 3669-3677Heddleston K L, Gallagher J E, Rebers P A. 1972. Fowlcholera: Gel diffusion precipitin test for serotypingPasteurella multocida from avian species. Avian Diseases,16, 925-936Johnson T J, Abrahante J E, Hunter S S, Hauglund M, TatumF M, Maheswaran S K, Briggs R E. 2013. Comparativegenome analysis of an avirulent and two virulent strainsof avian Pasteurella multocida reveals candidate genesinvolved in fitness and pathogenicity. BMC Microbiology,13, 106.Kim T J, Lee J, Lee B. 2006. Develpment of a toxA geneknock-out mutant of Pasteurella multocida and evaluationof its protective effects. Journal of Microbiology, 44,320-326Lainson F A, Dagleish M P, Fontaine M C, Bayne C, HodgsonJ C. 2013. Draft genome sequence of Pasteurella multocidaA:3 strain 671/90. Genome Announcements, 1, e00803-13May B J, Zhang Q, Li L L, Paustian M L, Whittam T S, KapurV. 2001. Complete genomic sequence of Pasteurellamultocida, Pm70. Proceedings of the National Academyof Sciences of the United States of America, 98, 3460-3465Steen J A, Steen J A, Harrison P, Seemann, Wilkie I, HarperM, Adler B, Boyce J D. 2010. Fis is essential for capsuleproduction in Pasteurella multocida and regulatesexpression of other important virulence factors. PLoSPathogens, 6, e1000750.Tatum F M, Tabatabai L B, Briggs R E. 2009a. Protection againstfowl cholera conferred by vaccination with recombinantPasteurella multocida filamentous hemagglutinin peptides.Avian Diseases, 53, 169-174Tatum F M, Tabatabai L B, Briggs R E. 2009b. Sialicacid uptake is necessary for virulence of Pasteurellamultocidain turkeys. Microbial Pathogenesis, 46, 337-344Tatum F M, Yersin A G, Briggs R E. 2005. Construction andvirulence of a Pasteurella multocida fhaB2 mutant inturkeys. Microbial Pathogenesis, 39, 9-17Wilson B A, Ho M. 2013. Pasteurella multocisa: from zoonosisto cellular microbiology. Clinical Microbiology Reviews,26, 631-654. |
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