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| The mRNA Expression Profiles of Five Heat Shock Protein Genes from Frankliniella occidentalis at Different Stages and Their Responses to Temperatures and Insecticides |
| WANG Hai-hong, Stuart R Reitz, WANG Li-xia, WANG Shuai-yu, LI Xue , LEI Zhong-ren |
1、State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100193, P.R.China
2、Malheur County Extension, Oregon State University, Ontario 97914, USA
3、The General Station of Plant Protection in Shandong, Jinan 250100, P.R.China |
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摘要 The western flower thrips, Frankliniella occidentalis (Pergande) is a highly invasive pest that is able to exploit many crops across a wide range of environmental conditions. Five full-length cDNAs of heat shock protein (HSP) genes (Fo-HSP90, Fo-HSP70, Fo-HSP60, Fo-HSP40 and Fo-HSP28.9) were cloned from F. occidentalis, and their expression profiles were investigated under conditions of thermal stress and insecticide exposure, and at different stages during development, using real-time quantitative PCR. All five gene sequences showed high similarity to homologs in other species, indicating the conserved function of this gene family. HSP60 represents an informative phylogenetic marker at the ordinal taxonomic level within Insecta, but HSP90, which has two homologous copies in Hymenoptera, was not informative. The expression of Fo-HSPs under thermal stress suggests that Fo-HSP90, Fo-HSP70, and Fo-HSP28.9 are inducible by both cold and heat stress, Fo-HSP40 is only heat-inducible, and Fo-HSP60 is thermally insensitive. There were two patterns of cold induction of Fo-HSPs: one is from 0 to 4°C and the other is around -8°C. All five Fo-HSPs genes were induced by exposure to sublethal concentrations of the insecticide avermectin. The expression of the five Fo-HSPs during different developmental stages suggests that they all play a role in development of F. occidentalis.
Abstract The western flower thrips, Frankliniella occidentalis (Pergande) is a highly invasive pest that is able to exploit many crops across a wide range of environmental conditions. Five full-length cDNAs of heat shock protein (HSP) genes (Fo-HSP90, Fo-HSP70, Fo-HSP60, Fo-HSP40 and Fo-HSP28.9) were cloned from F. occidentalis, and their expression profiles were investigated under conditions of thermal stress and insecticide exposure, and at different stages during development, using real-time quantitative PCR. All five gene sequences showed high similarity to homologs in other species, indicating the conserved function of this gene family. HSP60 represents an informative phylogenetic marker at the ordinal taxonomic level within Insecta, but HSP90, which has two homologous copies in Hymenoptera, was not informative. The expression of Fo-HSPs under thermal stress suggests that Fo-HSP90, Fo-HSP70, and Fo-HSP28.9 are inducible by both cold and heat stress, Fo-HSP40 is only heat-inducible, and Fo-HSP60 is thermally insensitive. There were two patterns of cold induction of Fo-HSPs: one is from 0 to 4°C and the other is around -8°C. All five Fo-HSPs genes were induced by exposure to sublethal concentrations of the insecticide avermectin. The expression of the five Fo-HSPs during different developmental stages suggests that they all play a role in development of F. occidentalis.
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Received: 02 July 2013
Accepted:
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| Fund: This research is partially funded by the National Natural Science Foundation of China (31201526), the National 973 Program of China (2009CB119000), the Earmarked Fund for Modern Agro- Industry Technology Research System (CARS-25-B-07) and the Special Fund for Agro-Scientific Research in the Public Interest of China (20090332). |
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Corresponding Authors:
LEI Zhong-ren, Tel: +86-10-62815930-801, Fax: +86-10-62815930, E-mail:
zrlei@ippcaas.cn
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| About author: WANG Hai-hong, Mobile: 18600942206, E-mail: hhwang@ippcaas.cn |
Cite this article:
WANG Hai-hong, Stuart R Reitz, WANG Li-xia, WANG Shuai-yu, LI Xue , LEI Zhong-ren.
2014.
The mRNA Expression Profiles of Five Heat Shock Protein Genes from Frankliniella occidentalis at Different Stages and Their Responses to Temperatures and Insecticides. Journal of Integrative Agriculture, 13(10): 2196-2210.
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