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| Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis |
| WANG Xiang, WANG Xin-guo, REN Jiang-ping, MA Ying, YIN Jun |
1.National Center for Wheat Research, Henan Agricultural University, Zhengzhou 450002, P.R.China
2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 The homeobox transcription factor WUSCHEL (WUS) plays a critical role in keeping the balance between the maintenance and differentiation of stem cell population in shoot and floral meristems of Arabidopsis thaliana. The corresponding gene SlWUS is yet to be characterized in tomato. In order to characterize SlWUS gene and its biological function, we cloned it from tomato and analyzed its structure. Tissue expression showed that the SlWUS highly expressed in tomato flower abscission zone. The overexpression of SlWUS in Arabidopsis could trigger undifferentiation of plant flower organ and indeterminacy of flower identity, suggesting that SlWUS maybe involved in flower structure development as well as flower organ identity. Taken together, our results indicated that the SlWUS plays an important role in flower abscission zone and plant organ shedding.
Abstract The homeobox transcription factor WUSCHEL (WUS) plays a critical role in keeping the balance between the maintenance and differentiation of stem cell population in shoot and floral meristems of Arabidopsis thaliana. The corresponding gene SlWUS is yet to be characterized in tomato. In order to characterize SlWUS gene and its biological function, we cloned it from tomato and analyzed its structure. Tissue expression showed that the SlWUS highly expressed in tomato flower abscission zone. The overexpression of SlWUS in Arabidopsis could trigger undifferentiation of plant flower organ and indeterminacy of flower identity, suggesting that SlWUS maybe involved in flower structure development as well as flower organ identity. Taken together, our results indicated that the SlWUS plays an important role in flower abscission zone and plant organ shedding.
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Received: 31 May 2011
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (30670188), the National Key Project for Researches on Transgenic Plant of China (2009ZX08002-011B) and the Education Department Project Of Henan Province, China (2010B210015). |
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Corresponding Authors:
Correspondence YIN Jun, Tel: +86-371-63558203, E-mail: xmzxyj@126.com, wxgyz@sohu.com
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| About author: WANG Xiang, Tel: +86-371-63558215, E-mail: wxgyz@sohu.com |
Cite this article:
WANG Xiang, WANG Xin-guo, REN Jiang-ping, MA Ying, YIN Jun.
2012.
Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis. Journal of Integrative Agriculture, 12(8): 1257-1265.
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