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| Overexpression of SOS Genes Enhanced Salt Tolerance in Sweetpotato |
| GAO Shang, ZHAI Hong, HE Shao-zhen, LIU Qing-chang |
| 1.Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China |
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摘要 The production of transgenic sweetpotato (cv. Xushu 18) plants exhibiting enhanced salt tolerance using salt overlysensitive (SOS) genes was achieved through Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strainEHA105 harbors a binary vector pCAMBIA3301 with SOS genes (SOS1, SOS2 and SOS3) and bar gene. Selection culturewas conducted using 0.3 mg L-1 phosphinothricin (PPT). A total of 40 plants were produced from the inoculated 170 cellaggregates via somatic embryogenesis. PCR analysis showed that 37 of the 40 regenerated plants were transgenic plants.The in vitro assay demonstrated that superoxide dismutase (SOD) and proline were significantly more accumulated andmalonaldehyde (MDA) was significantly less accumulated in 21 transgenic plants than in control plants when they wereexposed to 86 mmol L-1 NaCl. Salt tolerance of these 21 plants was further evaluated with Hoagland solution containing 0,51, 86, and 120 mmol L-1 NaCl in the greenhouse. The results indicated that 6 of them had significantly better growth androoting ability than the remaining 15 transgenic plants and control plants. Expression of SOS genes in the 6 salt-toleranttransgenic plants was demonstrated by RT-PCR analysis. This study provides an alternative approach for improving salttolerance of sweetpotato.
Abstract The production of transgenic sweetpotato (cv. Xushu 18) plants exhibiting enhanced salt tolerance using salt overlysensitive (SOS) genes was achieved through Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strainEHA105 harbors a binary vector pCAMBIA3301 with SOS genes (SOS1, SOS2 and SOS3) and bar gene. Selection culturewas conducted using 0.3 mg L-1 phosphinothricin (PPT). A total of 40 plants were produced from the inoculated 170 cellaggregates via somatic embryogenesis. PCR analysis showed that 37 of the 40 regenerated plants were transgenic plants.The in vitro assay demonstrated that superoxide dismutase (SOD) and proline were significantly more accumulated andmalonaldehyde (MDA) was significantly less accumulated in 21 transgenic plants than in control plants when they wereexposed to 86 mmol L-1 NaCl. Salt tolerance of these 21 plants was further evaluated with Hoagland solution containing 0,51, 86, and 120 mmol L-1 NaCl in the greenhouse. The results indicated that 6 of them had significantly better growth androoting ability than the remaining 15 transgenic plants and control plants. Expression of SOS genes in the 6 salt-toleranttransgenic plants was demonstrated by RT-PCR analysis. This study provides an alternative approach for improving salttolerance of sweetpotato.
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Received: 21 January 2011
Accepted:
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| Fund: This work was supported by China Agriculture ResearchSystem (Sweetpotato), the National High-Tech R&DProgram of China (2009AA10Z102), and the NationalTransgenic Plants Project of China (2009ZX08009-064B). |
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Corresponding Authors:
Correspondence LIU Qing-chang, Tel: +86-10-62733710, E-mail: liuqc@cau.edu.cn
E-mail: liuqc@cau.edu.cn
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| About author: GAO Shang, Tel: +86-10-62732559, E-mail: gaoshangd@126.com |
Cite this article:
GAO Shang, ZHAI Hong, HE Shao-zhen, LIU Qing-chang.
2012.
Overexpression of SOS Genes Enhanced Salt Tolerance in Sweetpotato. Journal of Integrative Agriculture, 12(3): 378-386.
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