Please wait a minute...
Journal of Integrative Agriculture
Advanced Online Publication | Current Issue | Archive | Adv Search
Genome-wide characterization and expression analysis of the cultivated peanut AhPR10 gene family mediating resistance to Aspergillus flavus L.

Qi Zhao1, 2*, Mengjie Cui1, 3*, Tengda Guo1, Lei Shi1, 3, Feiyan Qi1, 3, Ziqi Sun1, 3, Pei Du1, 3, Hua Liu1, 3, Yu Zhang1, Zheng Zheng1, 3, Bingyan Huang1, 3, Wenzhao Dong1, 3, Suoyi Han1, 3#, Xinyou Zhang1, 3#

1 Henan Academy of Agricultural Sciences/Postgraduate T & R Base of Zhengzhou University/Institute of Crop Molecular Breeding/The Shennong Laboratory/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture and Rural Affairs, China/Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou 450002, China

2 School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450066, China

3 National Centre for Plant Breeding, Xinxiang 453500, China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  病程相关蛋白PR10在植物生长、发育及胁迫响应中发挥重要作用本研究对栽培花生AhPR10基因家族进行了全基因组水平的系统鉴定,并对其系统进化关系、保守基序、基因结构、共线性等进行分析。共鉴定出54AhPR10基因,系统进化树将其分为八组,该结果已在基因结构和保守基序的特征分析中得到了证实。对其在染色体上的分布和共线性分析表明,片段复制事件AhPR10基因家族扩展的关键。此外,AhPR10基因具有组成型和诱导型两种表达模式。值得注意的是,AhPR10-7AhPR10-33AhPR10-41基因可能在花生抵御黄曲霉菌的侵染过程中发挥关键作用。体外抑菌实验表明AhPR10-33融合蛋白能有效抑制黄曲霉菌丝生长。本研究为深入剖析和明确AhPR10在花生黄曲霉抗性中的作用奠定分子信息基础。

Abstract  The pathogenesis-related protein PR10 is essential for plant growth, development, and stress responses.  In this study, PR10 genes in cultivated peanut (Arachis hypogaea L.) were systematically identified, after which their phylogenetic relationships, conserved motifs, gene structures, and syntenic relationships were analyzed.  A total of 54 AhPR10 genes were identified.  They were then divided into eight groups according to their phylogenetic relationships, which were supported by the characterization of gene structures and conserved motifs.  Analyses of chromosomal distribution and synteny revealed that segmental duplications were critical for the expansion of the AhPR10 gene family.  In addition, the identified AhPR10 genes had constitutive and inducible expression patterns.  Notably, AhPR10-7AhPR10-33, and AhPR10-41 may have crucial functions affecting the resistance of peanut to Aflavus.  In vitro fungistatic experiments indicated that recombinant AhPR10-33 can effectively inhibit Aflavus mycelial growth.  The study results provide useful insights for future research on AhPR10 functions that protect peanut from the detrimental effects of Aflavus.


Keywords:  cultivated peanut       PR10              phylogenetic analysis              expression pattern              Aspergillus flavus L.  
Online: 08 July 2024  
Fund: This work was supported by the National Key R&D Program of China (2022YFD1200400) and the National Natural Science Foundation of China (32301851).
About author:  #Correspondence Xinyou Zhang, E-mail: haasxinyou@163.com; Suoyi Han, E-mail: suoyi_han@126.com * These authors contributed equally to this study.

Cite this article: 

Qi Zhao, Mengjie Cui, Tengda Guo, Lei Shi, Feiyan Qi, Ziqi Sun, Pei Du, Hua Liu, Yu Zhang, Zheng Zheng, Bingyan Huang, Wenzhao Dong, Suoyi Han, Xinyou Zhang. 2024. Genome-wide characterization and expression analysis of the cultivated peanut AhPR10 gene family mediating resistance to Aspergillus flavus L.. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2024.07.006

Agarwal P, Agarwal P K. 2014. Pathogenesis related-10 proteins are small, structurally similar but with diverse role in stress signaling. Molecular Biology Reports, 41, 599-611.

Agarwal P, Bhatt V, Singh R, Das M, Sopory S K, Chikara J. 2013. Pathogenesis-related gene, JcPR-10a from Jatropha curcas exhibit RNase and antifungal activity. Molecular Biotechnology, 54, 412-425.

Arcos-Ortega G F, Chan-Kuuk R A, González-Kantún W A, Souza-Perera R A, Nakazawa-Ueji Y E, Avilés-Berzunza E, Godoy-Hernández G, Lawton M A, Aguilar J J. 2010. Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants. Electronic Journal of Biotechnology, 13, 7-8.

Bahramnejad B, Goodwin P H, Zhang J, Atnaseo C, Erickson L R. 2010. A comparison of two class 10 pathogenesis-related genes from alfalfa and their activation by multiple stresses and stress-related signaling molecules. Plant Cell Reports, 29, 1235-1250.

Bailey T L, Johnson J, Grant C E, Noble W S. 2015. The MEME Suite. Nucleic Acids Research, 43, W39-W49.

Brand Y, Hovav R. 2010. Identification of suitable internal control genes for quantitative Real-Time PCR expression analyses in peanut (Arachis hypogaea). Peanut Science, 37, 12-19.

Bertioli D J, Cannon S B, Froenicke L, Huang G, Farmer A D, Cannon E K, Liu X, Gao D, Clevenger J, Dash S, Ren L, Moretzsohn M C, Shirasawa K, Huang W, Vidigal B, Abernathy B, Chu Y, Niederhuth C E, Umale P, Araújo A C, et al. 2016. The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut. Nature Genetics, 48, 438-446.

Cannon S B, Mitra A, Baumgarten A, Young N D, May G. 2004. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana. BMC Plant Biology, 4, 10-30.

Chadha P, Das R H. 2006. A pathogenesis related protein, AhPR10 from peanut: An insight of its mode of antifungal activity. Planta, 225, 213-222.

Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13, 1194-1202.

Chen R, He H, Yang Y, Qu Y, Ge F, Liu D Q. 2017. Functional characterization of a pathogenesis-related protein family 10 gene, LrPR10-5, from Lilium regale Wilson. Australasian Plant Pathology, 46, 251-259.

Chen Z Y, Brown R L, Rajasekaran K, Damann K E, Cleveland T E. 2006. Identification of a maize kernel pathogenesis-related protein and evidence for its involvement in resistance to Aspergillus flavus infection and aflatoxin production. Phytopathology, 96, 87-95.

Cheng P, Jiang Y, Jin L, Wu J, Wang J, Xu P, Zhang S. 2013. Advances on Class 10 pathogenesis-related proteins. Soybean Science, 32, 415-419. (in Chinese)

Chou K C, Shen H B. 2008. Cell-PLoc: A package of web servers for predicting subcellular localization of proteins in various organisms. Nature Protocols, 3, 153-162.

Christensen A B, Cho B H, Næsby M, Gregersen P L, Brandt J, Madriz-Ordeñana K, Collinge D B, Thordal-Christensen H. 2002. The molecular characterization of two barley proteins establishes the novel PR-17 family of pathogenesis-related proteins. Molecular Plant Pathology, 3, 135-144.

Crowell D N, John M E, Russell D, Amasino R M. 1992. Characterization of a stress-induced, developmentally regulated gene family from soybean. Plant Molecular Biology, 18, 459-466.

Cui M, Han S, Wang D, Haider M S, Guo J, Zhao Q, Du P, Sun Z, Qi F, Zheng Z, Huang B, Dong W, Li P, Zhang X. 2022. Gene co-expression network analysis of the comparative transcriptome identifies hub genes associated with resistance to Aspergillus flavus L. in cultivated peanut (Arachis hypogaea L.). Frontiers in Plant Science, 13, 899177-899194.

Cui M, Haider M S, Chai P, Guo J, Du P, Li H, Dong W, Huang B, Zheng Z, Shi L, Zhang X, Han S. 2021. Genome-wide identification and expression analysis of AP2/ERF transcription factor related to drought stress in cultivated peanut (Arachis hypogaea L.). Frontiers in Genetics, 12, 750761-750777.

El-Banna A, Hajirezaei M R, Wissing J, Ali Z, Vaas L, Heine-Dobbernack E, Jacobsen H J, Schumacher H M, Kiesecker H. 2010. Over-expression of PR-10a leads to increased salt and osmotic tolerance in potato cell cultures. Journal of Biotechnology, 150, 277-287.

Finn R D, Clements J, Eddy S R. 2011. HMMER web server: Interactive sequence similarity searching. Nucleic Acids Research, 39, W29-W37.

Gao G, Zhang X, Zhao K, Zhao K, Cao D, Ma Q, Zhu S, Qu C, Ma Y, Gong F, Li Z, Ren R, Ma X L, Yin D. 2021. Genome wide identification and expression analysis of patatin-like protein family members in peanut (Arachis hypogaea L.). Reproduction and Breeding, 1, 48-54.

Gao Z, van de Weg W E, Schaart J G, Schouten H J, Tran D H, Kodde L P, van der Meer I M, van der Geest A H, Kodde J, Breiteneder H, Hoffmann-Sommergruber K, Bosch D, Gilissen L J. 2005. Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica). Theoretical and Applied Genetics, 111, 171-183.

Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel R D, Bairoch A. 2003. ExPASy: The proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Research, 31, 3784-3788.

Guo B, Chen X, Dang P, Scully BT, Liang X, Holbrook C C, Yu J, Culbreath A K. 2008. Peanut gene expression profiling in developing seeds at different reproduction stages during Aspergillus parasiticus infection. BMC Developmental Biology, 8, 12-27.

Hashimoto M, Kisseleva L, Sawa S, Furukawa T, Komatsu S, Koshiba T. 2004. A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant and Cell Physiology, 45, 550-559.

He H, Chen C, Han Q, Zhang N, Ge F, Liu D. 2014. Cloning and expression analysis of a pathogenesis-related protein 10 gene from Juglans sigillata. Plant Science Journal, 32, 612-619.

He M, Xu Y, Cao J, Zhu Z, Jiao Y, Wang Y, Guan X, Yang Y, Xu W, Fu Z. 2012. Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection. Protoplasma, 250, 129-140.

He Z, Zhang H, Gao S, Lercher M J, Chen W, Hu S. 2016. Evolview v2: An online visualization and management tool for customized and annotated phylogenetic trees. Nucleic Acids Research, 44, W236-W241.

Hoffmann-Sommergruber K, Vanek-Krebitz M, Radauer C, Wen J, Ferreira F, Scheiner O, Breiteneder H. 1997. Genomic characterization of members of the Bet v 1 family: Genes coding for allergens and pathogenesis-related proteins share intron positions. Gene, 197, 91-100.

Hu B, Jin J, Guo A, Zhang H, Luo J, Gao G. 2015. GSDS 2.0: An upgraded gene feature visualization server. Bioinformatics, 31, 1296-1297.

Huang J, Chang F, Wang C. 1997. Characterization of a lily tapetal transcript that shares sequence similarity with a class of intracellular pathogenesis-related (IPR) proteins. Plant Molecular Biology, 34, 681-686.

Huang W Y, Cai Y Z, Zhang Y. 2010. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutrition and Cancer, 62, 1-20.

Hwang S H, Lee I A, Yie S W, Hwang D J. 2008. Identification of an OsPR10a promoter region responsive to salicylic acid. Planta, 227, 1141-1150.

Jain S, Kumar D, Jain M, Chaudhary P, Deswal R, Sarin N B. 2012. Ectopic overexpression of a salt stress-induced pathogenesis-related class 10 protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco. Plant Cell Tissue and Organ Culture, 109, 19-31.

Kim S T, Yu S, Kang Y H, Kim S G, Kim J Y, Kim S H, Kang K Y. 2008. The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity. Plant Cell Reports, 27, 593-603.

Krishnaswamy S, Baral P K, James M N G, Kav N N V. 2011. Site-directed mutagenesis of histidine 69 and glutamic acid 148 alters the ribonuclease activity of pea ABR17 (PR10.4). Plant Physiology and Biochemistry, 49, 958-962.

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870-1874.

Lebel S, Schellenbaum P, Walter B, Maillot P. 2010. Characterization of the Vitis vinifera PR10 multigene family. BMC Plant Biology, 10, 184-196.

Lee O R, Pulla R K, Kim Y J, Balusamy S R, Yang D C. 2012. Expression and stress tolerance of PR10 genes from Panax ginseng C. A. Meyer. Molecular Biology Reports, 39, 2365-2374.

Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouzé P, Rombauts S. 2002. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 30, 325-327.

Liu D, Han Q, Shah T, Chen C, Wang Q, Tang B, Ge F. 2018. A hybrid proline-rich cell-wall protein gene JsPRP1 from Juglans sigillata Dode confers both biotic and abiotic stresses in transgenic tobacco plants. Trees, 32, 1199-1209.

Liu J, Dou H, Zhao G, Geng Z, Han S, An Z, Zhang H, Wang Y. 2022. Cloning and drought stress function analysis of pathogenesis-related proteins GbPR10 gene in sea-island cotton (Gossypium barbadense). Journal of Agricultural Biotechnology, 30, 272-283.

Liu J, Ekramoddoullah A K. 2006. The family 10 of plant pathogenesis-related proteins: Their structure, regulation, and function in response to biotic and abiotic stresses. Physiological and Molecular Plant Pathology, 68, 3-13.

Liu J, Ekramoddoullah A K, Piggott N, Zamani A. 2005. Molecular cloning of a pathogen/wound-inducible PR10 promoter from Pinus monticola and characterization in transgenic Arabidopsis plants. Planta, 221, 159-169.

Liu X, Huang B, Lin J, Fei J, Chen Z, Pang Y, Sun X, Tang K. 2006. A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress-and pathogen-inducible. Journal of Plant Physiology, 163, 546-556.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25, 402-408.

Lo S C, Hipskind J D, Nicholson R L. 1999. cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum. Molecular Plant (Microbe Interaction), 12, 479-489.

Loon L C V, Pierpoint W S, Boller T, Conejero V. 1994. Recommendations for naming plant pathogenesis-related proteins. Plant Molecular Biology Reporter, 12, 245-264.

Loon L C V, Rep M, Pieterse C M J. 2006. Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology, 44, 135-162.

Luo M, Dang P, Guo B, He G. 2005. Generation of expressed sequence tags (ESTs) for gene discovery and marker development in cultivated peanut. Crop Science, 45, 346-353.

Matton D P, Brisson N. 1989. Cloning, expression, and sequence conservation of pathogenesis-related gene transcripts of potato. Molecular Plant (Microbe Interaction), 2, 325-331.

Mcgee J D, Hamer J E, Hodges T K. 2001. Characterization of a PR-10 pathogenesis-related gene family induced in rice during infection with Magnaporthe grisea. Molecular Plant (Microbe Interactions), 14, 877-886.

Mistry J, Chuguransky S, Williams L, Qureshi M, Salazar G A, Sonnhammer E L L, Tosatto S C E, Paladin L, Raj S, Richardson L J, Finn R D, Bateman A. 2021. Pfam: The protein families database in 2021. Nucleic Acids Research, 49, D412-D419.

Park C J, Kim K J, Shin R, Park J M, Shin Y C, Paek K H. 2004. Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway. The Plant Journal (for Cell and Molecular Biology), 37, 186-198.

Potter S C, Luciani A, Eddy S R, Park Y, Lopez R, Finn R D. 2018. HMMER web server: 2018 update. Nucleic Acids Research, 46, W200-W204.

Samac D A, Penuela S, Schnurr J A, Hunt E N, Foster-Hartnett D, Vandenbosch K A, Gantt J S. 2011. Expression of coordinately regulated defence response genes and analysis of their role in disease resistance in Medicago truncatula. Molecular Plant Pathology, 12, 786-798.

Somssich I E, Schmelzer E, Kawalleck P, Hahlbrock K. 1988. Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley. Molecular Genetics and Genomics, 213, 93-98.

Su H, Jiao Y T, Wang F F, Liu Y E, Niu W L, Liu G T, Xu Y. 2018. Overexpression of VpPR10.1 by an efficient transformation method enhances downy mildew resistance in V. vinifera. Plant Cell Reports, 37, 819-832.

Thompson J D, Higgins D G, Gibson T J. 1994. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673-4680.

Van Loon L C, Rep M, Pieterse C M. 2006. Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology, 44, 135-162.

Vision T J, Brown D G, Tanksley S D. 2000. The origins of genomic duplications in Arabidopsis. Science, 290, 2114-2117.

Voorrips R E. 2002. MapChart: Software for the graphical presentation of linkage maps and QTLs. The Journal of Heredity, 93, 77-78.

Wang L, Sun Y, Li Y, Sun X, Wu Q, Cao D, Huang G, Xue J. 2021. Research progress of plant major latex protein. Journal of Anhui Agricultural Sciences, 49, 12-14. (in Chinese)

Wang Z, Yan L, Wan L, Huai D, Kang Y, Shi L, Jiang H, Lei Y, Liao B. 2019. Genome-wide systematic characterization of bZIP transcription factors and their expression profiles during seed development and in response to salt stress in peanut. BMC Genomics, 20, 51-64.

Wu F, Yan M, Li Y, Chang S, Song X, Zhou Z, Gong W. 2003. cDNA cloning, expression, and mutagenesis of a PR-10 protein SPE-16 from the seeds of Pachyrrhizus erosus. Biochemical and Biophysical Research Communications, 312, 761-766.

Xie C, Liang X, Li L, Liu H. 2009. Cloning and prokaryotic expression of ARAhPR10 gene with resistance to Aspergillus flavus in peanut. Genomics and Applied Biology, 28, 237-244. (in Chinese)

Xie C, Liu H, Li L, Liang. 2008. Advances on class 10 pathogenesis-related proteins. Molecular Plant Breeding, 6, 949-953. (in Chinese)

Xie Y, Chen Z, Browm R L, Bhatnagar D. 2010. Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays. Journal of Plant Physiology, 167, 121-130.

Xu P, Jiang L, Wu J, Li W, Fan S, Zhang S. 2014. Isolation and characterization of a pathogenesis-related protein 10 gene (gmpr10) with induced expression in soybean (Glycine max) during infection with Phytophthora sojae. Molecular Biology Reports, 41, 4899-4909.

Xu X, Guo S, Chen K, Song H, Liu J, Guo L, Qian Q, Wang H. 2010. A 796 bp PsPR10 gene promoter fragment increased root-specific expression of the GUS reporter gene under the abiotic stresses and signal molecules in tobacco. Biotechnology Letters, 32, 1533-1539.

Zandvakili N, Zamani M R, Motallebi M, Moghaddassi Jahromi Z. 2017. Cloning, overexpression and in vitro antifungal activity of Zea Mays PR10 Protein. Iranian Journal of Biotechnology, 15, 42-49.

Zhang J, Xiong A, Erickson L R. 2011. Isolation and characterization of a harvest-inducible gene hi11 and its promoter from alfalfa. Molecular Biology Reports, 38, 23-29.

Zhao L, Ma L, Wang Z, Chen M, Shen Y, Huang L. 2015. Molecular cloning and characterization of a pathogenesis-related protein SmPR10-1 from Salvia miltiorrhiza. Acta Physiologiae Plantarum, 37, 1-8.

Zhou X, Lu S, Xu Y, Wang J, Chen X. 2002. A cotton cDNA (GaPR-10) encoding a pathogenesis-related 10 protein with in vitro ribonuclease activity. Plant Science, 162, 629-636.

Zhu X, Leng X, Sun X, Mu Q, Wang B, Li X, Wang C, Fang J. 2015. Discovery of conservation and diversification of miR171 genes by phylogenetic analysis based on global genomes. Plant Genome, 8, 1-11.

Zhuang W, Chen H, Yang M, Wang J, Pandey M K, Zhang C, Chang W C, Zhang L, Zhang X, Tang R, Garg V, Wang X, Tang H, Chow C N, Wang J, Deng Y, Wang D, Khan A W, Yang Q, Cai T, et al. 2019. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication. Nature Genetics, 51, 865-876.

[1] Yuan Gao, Fuxia Bai, Qi Zhang, Xiaoya An, Zhaofei Wang, Chuzhao Lei, Ruihua Dang. Dynamic transcriptome profiles and novel markers in bovine spermatogenesis revealed by single-cell sequencing[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2362-2378.
[2] Shuang Cheng, Zhipeng Xing, Chao Tian, Mengzhu Liu, Yuan Feng, Hongcheng Zhang.

Optimized tillage methods increase mechanically transplanted rice yield and reduce the greenhouse gas emissions [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1150-1163.

[3] Liyao Su, Min Wu, Tian Zhang, Yan Zhong, Zongming (Max) Cheng.

Identification of key genes regulating the synthesis of quercetin derivatives in Rosa roxburghii through integrated transcriptomics and metabolomics [J]. >Journal of Integrative Agriculture, 2024, 23(3): 876-887.

[4] Mosses Lufuke, Xu Tian.

Women’s empowerment and food consumption: Evidence from female-headed households in Tanzania [J]. >Journal of Integrative Agriculture, 2024, 23(2): 457-467.

[5] Binbin Li, Xianmin Chen, Tao Deng, Xue Zhao, Fang Li, Bingchao Zhang, Xin Wang, Si Shen, Shunli Zhou.

Timing effect of high temperature exposure on the plasticity of internode and plant architecture in maize [J]. >Journal of Integrative Agriculture, 2024, 23(2): 551-565.

[6] ZHUANG Hui, LAN Jin-song, YANG Qiu-ni, ZHAO Xiao-yu, LI Yu-huan, ZHI Jing-ya, SHEN Ya-lin, HE Guang-hua, LI Yun-feng. SUPER WOMAN 2 (SPW2) maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3, OsMADS58, OsMADS13, and DROOPING LEAF[J]. >Journal of Integrative Agriculture, 2024, 23(1): 59-76.
[7] WANG Xue-feng, SHAO Dong-nan, LIANG Qian, FENG Xiao-kang, ZHU Qian-hao, YANG Yong-lin, LIU Feng, ZHANG Xin-yu, LI Yan-jun, SUN Jie, XUE Fei. A 2-bp frameshift deletion at GhDR, which encodes a B-BOX protein that co-segregates with the dwarf-red phenotype in Gossypium hirsutum L.[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2000-2014.
[8] GAO Yue, LUO Jian, SUN Yue, ZHANG Hua-wei, ZHANG Da-xia, LIU Feng, MU Wei, LI Bei-xing. Photosensitivity and a precise combination of size-dependent lambda-cyhalothrin microcapsules synergistically generate better insecticidal efficacy [J]. >Journal of Integrative Agriculture, 2023, 22(5): 1477-1488.
[9] JIN Ji-su, LIU Yi-ran, ZHOU Zhong-shi, WAN Fang-hao, GUO Jian-ying. Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera: Chrysomelidae)[J]. >Journal of Integrative Agriculture, 2023, 22(3): 812-824.
[10] WANG Fei-bing, WAN Chen-zhong, NIU Hao-fei, QI Ming-yang, LI Gang, ZHANG Fan, HU Lai-bao, YE Yu-xiu, WANG Zun-xin, PEI Bao-lei, CHEN Xin-hong, YUAN Cai-yuan.

OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice [J]. >Journal of Integrative Agriculture, 2023, 22(2): 341-359.

[11] PAN Fan, GAO Li-jie, ZHU Kai-hui, DU Gui-lin, ZHU Meng-meng, ZHAO Li, GAO Yu-lin, TU Xiong-bing, ZHANG Ze-hua. Regional selection of insecticides and fungal biopesticides to control aphids and thrips and improve the forage quality of alfalfa crops[J]. >Journal of Integrative Agriculture, 2023, 22(1): 185-194.
[12] ZHANG Yu-zheng, XU Chen, LU Wen-li, WANG Xiao-zhe, WANG Ning, MENG Xiang-guang, FANG Yu-hui, TAN Qiu-ping, CHEN Xiu-de, FU Xi-ling, LI Ling.

PpMAPK6 regulates peach bud endodormancy release through interactions with PpDAM6 [J]. >Journal of Integrative Agriculture, 2023, 22(1): 139-148.

[13] GUO Jie, ZHOU Xu-tao, DAI Ke-li, YUAN Xiang-yang, GUO Ping-yi, SHI Wei-ping, ZHOU Mei-xue. Comprehensive analysis of YABBY gene family in foxtail millet (Setaria italica) and functional characterization of SiDL[J]. >Journal of Integrative Agriculture, 2022, 21(10): 2876-2887.
No Suggested Reading articles found!