Baseline establishment, susceptibility monitoring and risk assessment of cyproflanilide, a novel meta-diamide insecticide, against Chilo suppressalis (Lepidoptera: Crambidae) in China

doi:10.1016/j.jia.2025.03.028

Journal of Integrative Agriculture

2025, Vol. 24

Issue (11): 4342-4354 DOI: 10.1016/j.jia.2025.03.028

Plant Protection

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Baseline establishment, susceptibility monitoring and risk assessment of cyproflanilide, a novel meta-diamide insecticide, against Chilo suppressalis (Lepidoptera: Crambidae) in China

Wenchao Ge^*, Songtao Qiao^*, Chong Liu, Fangrui Guo, Shuai Wang, Hao Sun, Yan Liu, Fengxia Yang, Shunfan Wu, Congfen Gao^#

College of Plant Protection/State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, China

Highlights

• The baseline of susceptibility of Chilo suppressalis to cyproflanilide was established as LD₅₀=0.122 ng/larva by topical application and LC₅₀=0.026 mg L^–1 by the rice-seedling dipping method.
• The realized heritability (h²=0.067) indicates a low risk of resistance to cyproflanilide after 19 generations of selection.
• The field populations of chlorantraniliprole-resistant C. suppressalis show no cross-resistance to cyproflanilide.
• Cyproflanilide can serve as a novel alternative insecticide for controlling chlorantraniliprole-resistant populations of C. suppressalis in China.

Abstract
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摘要

二化螟是水稻种植区最严重的害虫之一，并且已对目前田间使用的大多数杀虫剂产生了不同程度的抗性。环丙氟虫胺是一种新型间二酰胺类杀虫剂，对多种害虫表现出高效活性。评估二化螟对环丙氟虫胺的抗性风险对其制定预防性抗性治理策略是必要的。在此，我们通过稻苗浸渍法和点滴法建立了二化螟对环丙氟虫胺的毒力敏感基线，其LC₅₀和LD₅₀值分别为0.026 mg/L和0.122 ng/头。环丙氟虫胺对37个二化螟田间种群的LC₅₀值范围为0.012至0.061 mg/L，其中25个田间种群对氯虫苯甲酰胺表现出不同程度的抗性，最高LC₅₀值为3770.059 mg/L。此外，种群敏感性分布模型分析表明，0.048 mg/L的环丙氟虫胺即可杀死90%的田间氯虫苯甲酰胺抗性二化螟种群，而氯虫苯甲酰胺需要2087.764 mg/L才能达到类似的防治效果。经过19代的抗性筛选，未发现二化螟对环丙氟虫胺的抗性显著上升（抗性倍数RR=3.1）。通过阈性状分析估计的抗性现实遗传力（h²）为0.067，表明在二化螟敏感品系中对环丙氟虫胺具有较低的抗性风险。环丙氟虫胺筛选种群未表现出明显的适合度代价（相对适合度=0.96），并且对七种杀虫剂的敏感性没有显著变化。这些结果表明，环丙氟虫胺是治理氯虫苯甲酰胺抗性二化螟的一种有效的替代杀虫剂。此外，抗性风险评估为环丙氟虫胺对二化螟的可持续抗性管理提供了科学应用指南。

Abstract

The rice stem borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is one of the most serious pests in rice-growing areas, and it has developed resistance to most insecticides currently used in the field. Cyproflanilide is a novel meta-diamide insecticide that has shown high activities to multiple pests. Evaluating the risk of resistance to cyproflanilide in C. suppressalis is necessary for its preventive resistance management. Here we established the baseline susceptibility of C. suppressalis to cyproflanilide by the rice-seedling dipping method and topical application, and the LC₅₀ and LD₅₀valueswere 0.026 mg L^–1 and 0.122 ng/larva, respectively. The LC₅₀ values of cyproflanilide in 37 field populations ranged from 0.012 to 0.061 mg L^–1, and 25 field populations exhibited resistance to chlorantraniliprole with the highest LC₅₀ value of 3,770.059 mg L^–1. In addition, a logistic distribution model analysis indicated that only 0.048 mg L^–1 of cyproflanilide was required to kill 90% field chlorantraniliprole-resistant populations of C. suppressalis, compared to 2,087.764 mg L^–1 of chlorantraniliprole for a similar level of control. Resistance screening over 19 generations did not result in resistance to cyproflanilide (RR=3.1-fold). The realized heritability (h²) of resistance was estimated as 0.067 by using threshold trait analysis, suggesting a low risk of cyproflanilide resistance development in susceptible strains. The Cypro-SEL population (F₁₀) had no obvious fitness cost (relative fitness=0.96), and no significant changes in sensitivity to seven tested insecticides. These findings suggested that cyproflanilide is a promising insecticide for the management of chlorantraniliprole-resistant C. suppressalis. Moreover, this integrated risk assessment provides scientific application guidelines for the sustainable resistance management of cyproflanilide for controlling C. suppressalis.

Keywords: cyproflanilide Chilo suppressalis susceptibility baseline risk assessment realized heritability

Received: 02 December 2024 Accepted: 18 February 2025 Online: 27 March 2025

Fund:

This study was funded by the National Key Research and Developmental Program of China (2022YFD1700200).

About author: Wenchao Ge, E-mail: 1130467864@qq.com; #Correspondence Congfen Gao, Tel: +86-25-84395244, E-mail: gaocongfen@njau.edu.cn * These authors contributed equally to this study.

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Wenchao Ge, Songtao Qiao, Chong Liu, Fangrui Guo, Shuai Wang, Hao Sun, Yan Liu, Fengxia Yang, Shunfan Wu, Congfen Gao. 2025. Baseline establishment, susceptibility monitoring and risk assessment of cyproflanilide, a novel meta-diamide insecticide, against Chilo suppressalis (Lepidoptera: Crambidae) in China. Journal of Integrative Agriculture, 24(11): 4342-4354.

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