Please wait a minute...
Journal of Integrative Agriculture  2013, Vol. 12 Issue (10): 1807-1815    DOI: 10.1016/S2095-3119(13)60499-2
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Impact Assessments of Transgenic cry1Ab Rice on the Population Dynamics of Five Non-Target Thrips Species and Their General Predatory Flower Bug in Bt and Non-Bt Rice Fields Using Color Sticky Card Traps
 Akhtar Zunnu Raen, YE Gong-yin, LU Zeng-bin, CHANG Xue, SHEN Xiao-jing, PENG Yu-fa
1 State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology, Ministry of Agriculture/Institute of Insect Sciences, Zhejiang
University, Hangzhou 310058, P.R.China
2 State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100094, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  A 2-yr field study was conducted to assess the effects of transgenic japonica rice (KMD1 and KMD2) with a synthetic cry1Ab gene from Bacillus thuingiensis Berliner on population dynamics and seasonal average densities of five thrips species including Stenchaetothrips biformis (Bagnall), Frankliniella intonsa (Trybom), F. tenuicornis (Uzel), Haplothrips aculeatus (Fabricius), Haplothrips tritici (Kurd) and their general predatory flower bug, Orius similis Zheng as compared to the parental control rice line using the white, blue and yellow sticky card traps. Population dynamics and seasonal average densities of these five thrips species and their general predatory flower bug were not significantly affected by rice type. Additionally, the white sticky card trap was suggested to be the most suitable for monitoring the population of these five thrips species and their general predator. These results show that our tested Bt rice lines do not interrupt the population of non-target thrips species and their general predatory flower bug in the field, and also cannot result in more occurrence of these thrips species in the rice ecosystem.

Abstract  A 2-yr field study was conducted to assess the effects of transgenic japonica rice (KMD1 and KMD2) with a synthetic cry1Ab gene from Bacillus thuingiensis Berliner on population dynamics and seasonal average densities of five thrips species including Stenchaetothrips biformis (Bagnall), Frankliniella intonsa (Trybom), F. tenuicornis (Uzel), Haplothrips aculeatus (Fabricius), Haplothrips tritici (Kurd) and their general predatory flower bug, Orius similis Zheng as compared to the parental control rice line using the white, blue and yellow sticky card traps. Population dynamics and seasonal average densities of these five thrips species and their general predatory flower bug were not significantly affected by rice type. Additionally, the white sticky card trap was suggested to be the most suitable for monitoring the population of these five thrips species and their general predator. These results show that our tested Bt rice lines do not interrupt the population of non-target thrips species and their general predatory flower bug in the field, and also cannot result in more occurrence of these thrips species in the rice ecosystem.
Keywords:  rice thrips       predatory flower bug       non-target effects       Bt rice       risk assessment  
Received: 21 December 2012   Accepted:
Fund: 

Financial supports were provided from the Special Research Projects for Developing Transgenic Plants, China (2013ZX08011- 001) and the China National Science Fund for Innovative Research Groups of Biological Control (31021003) as well the National 973 Program of China (2007CB109202).

Corresponding Authors:  Correspondence YE Gong-yin, Tel: +86-571-88982696, E-mail: chu@zju.edu.cn     E-mail:  chu@zju.edu.cn

Cite this article: 

Akhtar Zunnu Raen, YE Gong-yin, LU Zeng-bin, CHANG Xue, SHEN Xiao-jing, PENG Yu-fa. 2013. Impact Assessments of Transgenic cry1Ab Rice on the Population Dynamics of Five Non-Target Thrips Species and Their General Predatory Flower Bug in Bt and Non-Bt Rice Fields Using Color Sticky Card Traps. Journal of Integrative Agriculture, 12(10): 1807-1815.

[1]Akhtar Z R, Tian J C, Chen Y, Fang Q, Hu C, Chen M, Peng Y F, Ye G Y. 2010. Impacts of six Bt rice lines on nontarget rice feeding thrips under laboratory and field conditions. Environmental Entomology, 39, 715-726

[2]Akhtar Z R, Chen Y, Ye G Y. 2011. Bio-safety concerns of Bt rice in China. Global Journal of Environmental Science and Technology, 2, 1-8

[3]Bai Y Y, Jiang M X, Cheng J A, Wang D. 2006. Effects of cry1Ab toxin on Propylea japonica (Thunberg) (Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens Stål (Homoptera: Delphacidae), feeding on transgenic Bt rice. Environmental Entomology, 35, 1130- 1136.

[4]Bernal C C, Aguda R M, Cohen M B. 2002. Effect of rice lines transformed with Bacillus thuringiensis toxin genes on the brown planthopper and its predator Cyrtorhinus lividipennis. Entomologia Experimentalis et Applicata, 102, 21-28

[5]Chen T Y, Chu C C, Thomas J H, Kai U. 2004. Monitoring and trapping insects on poinsettia with yellow sticky card traps equipped with light emitting diodes. Hortechnology, 14, 337-341

[6]Chen M, Ye G Y, Lu X M, Hu C, Peng Y F, Shu Q Y, Illimar A. 2005. Biotransfer and bioaccumulation of Cry1Ab insecticidal protein in rice plant-brown planthopper-wolf spider food chain. Acta Entomologica Sinica, 48, 208-213

[7](in Chinese) Chen M, Zhao J Z, Ye G Y, Fu Q, Shelton A M. 2006. Impact of insect-resistant transgenic rice on target insect pests and non-target arthropods in China. Insect Science, 13, 409-420

[8]Chen M, Shelton A, Ye G Y. 2011. Insect-resistant genetically modified rice in China: from research to commercialization. Annual Review of Entomology, 56, 81-101

[9]Conner A J, Glare T R, Nap J P. 2003. The release of genetically modified crops into the environment-part II. Overview of ecological risk assessment. The Plant Journal, 33, 19-46

[10]Chen Y, Tian J C, Wang W, Fang Q, Akhtar Z R, Peng Y F, Hu C, Guo Y Y, Song Q S, Ye G Y. 2012. Bt rice expressing Cry1Ab does not stimulate an outbreak of its non-target herbivore, Nilaparvata lugens. Transgenic Research, 21, 279-291

[11]Chu C C, Pinter Jr P J, Henneberry T J, Umeda K, Natwick E T, Wei Y A, Reddy V R, Shrepatis M. 2000. Use of CC traps with different trap base colors for silver leaf whiteflies (Homoptera: Aleyrodidae), thrips (Thysanoptera: Thripidae), and leafhoppers (Homoptera: Cicadellidae). Journal of Economic Entomology, 93, 1329-1337

[12]Craig W, Tepfer M, Degrassi G, Ripandelli D. 2008. An overview of general features of risk assessments of genetically modified crops. Euphytica, 164, 853-880

[13]Dale D. 1994. Insect pests of the rice plant - their biology and ecology. In: Heinrichs E A, ed., Biology and Management of Rice Insects. Wiley Eastern/New Age International Limited, New Delhi, India. pp. 363-485

[14]Dale P J, Clarke B, Fontes, E M G. 2002. Potential for the environmental impact of transgenic crops. Nature Biotechnology, 20, 567-574

[15]Edge J M, Benedict J H, Carroll J P, Reding H K. 2001. Bollgard cotton: an assessment of global economic, environmental and social benefits. Journal of Cotton Science, 5, 121-136

[16]FAO (Food and Agriculture Organization). 2008. FAO and sustainable intensification of rice production for food security. [2012-06-15] ftp://ext-ftp.fao.org/Radio/ Scripts/2008/Rice-Prod.pdf Maclean J L, Dawe D C, Hardy B, Hettel G P. 2002. Rice Almanac. 3rd ed. CABI Publishing, Wallingford, Oxon. pp. 59-235

[17]Gao M Q, Hou S P, Pu D Q, Shi M, Ye G Y, Chen X X. 2010. Multi-generation effects of Bt rice on Anagrus nilaparvatae, a parasitoid of the nontarget pest Nilapavarta lugens. Environmental Entomology, 39, 2039- 2044.

[18]Hernandez L M. 1999. A review of the economically important species of the genus Orius (Heteroptera: Anthocoridae) in East Africa. Journal of Natural History, 33, 543-568

[19]High S M, Cohen M B, Shu Q Y, Illimar A. 2004. Achieving successful deployment of Bt rice. Trends in Plant Science, 9, 286-292

[20]James C. 2011. Global status of commercialized biotech/GM crops: 2011. In: ISAAA Brief, NO. 43. ISAAA, Ithaca. Khush G S. 2005. What it will take to feed 5.0 billion rice consumers in 2030. Plant Molecular Biology, 59, 1-6

[21]Lu Y H, Wu K M, Jiang Y Y, Guo Y Y, Desneux N. 2012. Widespread adoption of Bt cotton and insecticide decrease promotes biocontrol services. Nature, 487, 362- 365.

[22]MAPRC (Ministry of Agriculture of the People’s Republic of China). 2009. The second list of approval agricultural genetically modified organisms’ safety certificates in 2009. [2013-02-21] http://www.stee.agri.gov.cn/ biosafety/spxx/P020091127591594596689.pdf (in Chinese)

[23]Nap J P, Metz P L J, Escaler M, Conner A J. 2003. The release of genetically modified crops into the environment-part I. Overview of current status and regulations. The Plant Journal, 33, 1-18

[24]Naranjo S E. 2005. Long-term assessment of the effects of transgenic Bt cotton on the abundance of non-target arthropod natural enemies. Environmental Entomology, 34, 1193-1210

[25]Naranjo S E, Head G, Dively G. 2005. Field studies assessing arthropod nontarget effects in Bt transgenic crops: Introduction. Environmental Entomology, 34, 1178-1180

[26]Nugaliyadde L, Heinrichs E A. 1984. Biology of rice thrips, Stenchaetothrips biformis (Bagnall) (Thysanoptera: Thripidae), and a greenhouse rearing technique. Journal of Economic Entomology, 77, 1171-1175

[27]Pathak M D, Khan Z R. 1994. Insect Pests of Rice. International Rice Research Institute, Manila, Philippines. Ranamukhaarachchi S L, Wickramarachchi K S. 2007. Color preference and sticky traps for field management of thrips Ceratothripoides claratris (Shumsher) (Thysanoptera: Thripdae) in tomato in central Thailand. International Journal of Agriculture and Biology, 9, 839-844

[28]Ripa R, Funderburk J, Rodriguez F, Espinoza F, Mound L. 2009. Population abundance of Frankliniella occidentalis (Thysanoptera: Thripidae) and natural enemies on plant hosts in central Chile. Environmental Entomology, 38, 333-344

[29]Romeis J, Michael M, Franz B. 2006. Transgenic crops expressing Bacillus thuringiensis toxins and biological control. Nature Biotechnology, 24, 63-71

[30]Romeis J, Bartsch D, Bigler F, Candolfi M P, Gielkens M M C, Hartley S E, Hellmich R L, Huesing J E, Jepson P C, Layton R, et al. 2008. Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nature Biotechnology, 26, 203-208

[31]SAS Institute. 2001. PROC User’s Manual. 3rd ed. SAS Institute, Cary, NC. Sengonca C, Ahmadi K, Blaeser P. 2008. Biological characteristics of Orius similis Zheng (Heteroptera, Anthocoridae) by feeding on different aphid species as prey. Journal of Plant Diseases and Protection, 115, 32- 38.

[32]Shipp J L, Zariffa N. 1991. Spatial patterns of and sampling methods for western flower thrips (Thysanoptera: Thripidae) on greenhouse sweet pepper. The Canadian Entomologist, 123, 989-1000

[33]Shu Q Y, Ye G Y, Cui H R, Cheng X Y, Xiang Y B, Wu D X, Gao M W, Xia Y W, Hu C, Sardana R, et al. 2000. Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight Lepidopteran rice pest species. Molecular Breeding, 6, 433-439

[34]Tang Q Y, Feng M G. 2007. DPS Data Processing for Practical Statistics. Science Press, Beijing, China. (in Chinese)

[35]Tian J C, Liu Z C, Chen M, Chen Y, Chen X X, Peng Y F, Hu C, Ye G Y. 2010. Laboratory and field assessments of prey-mediated effects of transgenic Bt rice on Ummeliata insecticeps (Araneida: Linyphiidae). Environmental Entomology, 39, 1369-1377

[36]Tian J C, Chen Y, Li Z L, Li K, Chen M, Peng Y F, Hu C, Shelton A M, Ye G Y. 2012. Transgenic cry1Ab rice does not impact ecological fitness and predation of a generalist spider. PLoS ONE, e35164, 1-7

[37]Tu J M, Zhang G A, Datta K, Xu C G, He Y Q, Zhang Q F, Khush G S, Datta S K. 2000. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin. Nature Biotechnology, 18, 1101-1104

[38]UC IPM Online. 2013. Thrips Management Guidelines. [2013-1-10] http://www.ipm.ucdavis.edu/PMG/ PESTNOTES/pn7429.html Wei Y A, Doumbouya A D, Zeng D Q, Dong Y H, Reddy V R, Chu C C, Henneberr T J. 1999. Selectivity of CC traps in catching green leafhoppers and thrips. Guangxi Sciences, 6, 65-68. (in Chinese)

[39]Yang H, Li J X, Guo S D, Chen X J, Fan Y L, 1989. Transgenic rice plants produced by direct uptake of δ-endotoxin protein gene from Bacillus thuringiensis into rice protoplasts. Scientia Agricultura Sinica, 22, 1-5 (in Chinese)

[40]Ye G Y, Shu Q Y, Yao H W, Cui H R, Cheng X Y, Hu C, Xia Y W, Gao M W, Altossar I. 2001. Field evaluation of resistance of transgenic rice containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to two stem borers. Journal of Economic Entomology, 94, 270-276

[41]Ye G Y, Yao H W, Shu Q Y, Cheng X Y, Hu C, Xia Y W, Gao M W, Altosaar I. 2003. High levels of stable resistance in transgenic rice with a synthetic cry1Ab gene from Bacillus thuringiensis Berliner to rice leaffolder, Cnaphalocrocis medinalis (Guenée) under field conditions. Crop Protection, 22, 171-178

[42]Yu H L, Li Y H, Wu K M. 2011. Risk assessment and ecological effects of transgenic Bacillus thuringiensis crops on non-target organism. Journal of Integrative Plant Biology, 53, 520-538

[43]Zhou X M, Lei C L. 2002. Utilization efficiency and functional response of Orius similis Zheng (Hemiptera: Anthocoridae) to different preys. Acta Ecologica Sinica, 22, 2085-2090.
[1] ZHAO Man, LI Yun-he, NIU Lin-lin, CHEN Lin, LIANG Ge-mei . Assessment of the potential toxicity of insecticidal compounds to Peristenus spretus, a parasitoid of mirid bugs[J]. >Journal of Integrative Agriculture, 2022, 21(5): 1424-1435.
[2] LIU Yan-min, LI Yun-he, CHEN Xiu-ping, SONG Xin-yuan, SHEN Ping, PENG Yu-fa. No detrimental effect of Bt maize pollen containing Cry1Ab/2Aj or Cry1Ac on adult green lacewings Chrysoperla sinica Tjeder[J]. >Journal of Integrative Agriculture, 2019, 18(4): 893-899.
[3] WANG Yu-jiao, NIE Ji-yun, YAN Zhen, LI Zhi-xia, CHENG Yang, Saqib Farooq. Multi-mycotoxin exposure and risk assessments for Chinese consumption of nuts and dried fruits[J]. >Journal of Integrative Agriculture, 2018, 17(07): 1676-1690.
[4] YANG Gui-ling, WANG Wen, LIANG Sen-miao, YU Yi-jun, ZHAO Hui-yu, WANG Qiang, QIAN Yongzhong. Pesticide residues in bayberry (Myrica rubra) and probabilistic risk assessment for consumers in Zhejiang, China[J]. >Journal of Integrative Agriculture, 2017, 16(09): 2101-2109.
[5] Akhtar Zunnu Raen, DANG Cong, WANG Fang, PENG Yu-fa, YE Gong-yin. Thrips-mediated impacts from transgenic rice expressing Cry1Ab on ecological fitness of non-target predator Orius tantilus (Hemiptera: Anthocoridae)[J]. >Journal of Integrative Agriculture, 2016, 15(9): 2059-2069.
[6] NIE Ji-yun, KUANG Li-xue, LI Zhi-xia, XU Wei-hua, WANG Cheng, CHEN Qiu-sheng, LI An, ZHAO Xu-bo, XIE Han-zhong, ZHAO Duo-yong, WU Yong-long, CHENG Yang. Assessing the concentration and potential health risk of heavy metals in China’s main deciduous fruits[J]. >Journal of Integrative Agriculture, 2016, 15(7): 1645-1655.
[7] LI Bo, LIU Ji-fang, YANG Jun-xing, MA Yi-bing, cHEN Shi-bao. comparison of phytotoxicity of copper and nickel in soils with different chinese plant species[J]. >Journal of Integrative Agriculture, 2015, 14(6): 1192-1201.
[8] LI Zhi-xia, NIE Ji-yun, YAN Zhen, XU Guo-feng, LI Hai-fei, KUANG Li-xue, PAN Li-gang, XIE Han-zhong, WANG Cheng, LIU Chuan-de, ZHAO Xu-bo, GUO Yong-ze. Risk assessment and ranking of pesticide residues in Chinese pears[J]. >Journal of Integrative Agriculture, 2015, 14(11): 2328-2339.
[9] CHEN Yang, LAI Feng-xiang, SUN Yan-qun, HONG Li-ying, TIAN Jun-ce, ZHANG Zhi-tao, FU Qiang. Cry1Ab rice does not impact biological characters and functional response of Cyrtorhinus lividipennis preying on Nilaparvata lugens eggs[J]. >Journal of Integrative Agriculture, 2015, 14(10): 2011-2018.
[10] LAI Yun-song, HUANG Hai-qing, XU Meng-yun, WANG Liang-chao, ZHANG Xiao-bo, ZHANG Ji-wen , TU Ju-min. Development of Insect-Resistant Hybrid Rice by Introgressing the Bt Gene from Bt Rice Huahui 1 into II-32A/B, a Widely Used Cytogenic Male Sterile System[J]. >Journal of Integrative Agriculture, 2014, 13(10): 2081-2090.
[11] SHI Zhao-peng, DU Shang-gen, YANG Guo-qing, LU Zhen-zhen , WU Jin-cai. Effects of Pesticide Applications on the Biochemical Properties of Transgenic cry 2A Rice and the Life History Parameters of Nilaparvata lugens Stål (Homptera: Delphacidae)[J]. >Journal of Integrative Agriculture, 2013, 12(9): 1606-1613.
[12] CHENG Yong-xiang, HUANG Jing-feng, HAN Zhong-ling, GUO Jian-ping, ZHAO Yan-xia, WANG Xiu-zhen , GUO Rui-fang. Cold Damage Risk Assessment of Double Cropping Rice in Hunan, China[J]. >Journal of Integrative Agriculture, 2013, 12(2): 352-363.
[13] XU Lei, ZHANG Qiao, ZHOU Ai-lian, HUO Ran. Assessment of Flood Catastrophe Risk for Grain Production at the Provincial Scale in China Based on the BMM Method[J]. >Journal of Integrative Agriculture, 2013, 12(12): 2310-2320.
[14] HAN Yu, XU Xue-liang, MA Wei-hua, YUAN Ben-qi, WANG Hui, LIU Fang-zhou, WANG Man-qun, WU . The Influence of Transgenic cry1Ab/cry1Ac, cry1C and cry2A Rice on Non- Target Planthoppers and Their Main Predators Under Field Conditions [J]. >Journal of Integrative Agriculture, 2011, 10(11): 1739-1747.
[15] SONG Yu-feng, LU Xiao, REN Feng-shan. Variability of Pesticide Residues in Vegetables from the Marketplaces in Jinan City[J]. >Journal of Integrative Agriculture, 2011, 10(10): 1646-1652.
No Suggested Reading articles found!