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Journal of Integrative Agriculture  2019, Vol. 18 Issue (4): 893-899    DOI: 10.1016/S2095-3119(17)61887-2
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No detrimental effect of Bt maize pollen containing Cry1Ab/2Aj or Cry1Ac on adult green lacewings Chrysoperla sinica Tjeder
LIU Yan-min1, LI Yun-he1, CHEN Xiu-ping1, SONG Xin-yuan2, SHEN Ping3, PENG Yu-fa1
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 Jilin Academy of Agricultural Sciences, Changchun 130124, P.R.China
3 Science and Technology Development Center, Ministry of Agriculture, Beijing 100122, P.R.China
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Adult Chrysoperla sinica Tjeder is a common pollen feeder in maize fields.  They are thus directly exposed to insecticidal proteins by consumption of genetically engineered maize pollen containing Bacillus thuringiensis (Bt) proteins.  Here we assessed the potential effects of Cry1Ab/2Aj- or Cry1Ac-containing Bt maize pollen on the fitness of adult C. sinica via a dietary-exposure assay under laboratory conditions.  Survival, pre-oviposition, fecundity and adult dry weight did not differ between adult C. sinica consuming Bt or the corresponding non-Bt maize pollen.  The stability of the Cry protein in the food sources and uptake of the Cry protein by adult C. sinica during the feeding experiment were confirmed by ELISA.  These results demonstrate that adult C. sinica are not affected by the consumption of Cry1Ab/2Aj- or Cry1Ac-containing maize pollen, suggesting that production of Bt maize expressing cry1Ab/2Aj or cry1Ac genes will pose a negligible risk to adult C. sinica.
Keywords:  non-target effect        environmental risk assessment        ELISA        dietary exposure assay  
Received: 14 September 2017   Accepted: 01 April 2019
Fund: The study was supported by the National GMO New Variety Breeding Program of China (2015ZX08013-003).
Corresponding Authors:  Corrrespondence LI Yun-he, Tel: +86-10-62815947, Fax: +86-10-62896114, E-mail:   
About author:  LIU Yan-min, E-mail:;

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LIU Yan-min, LI Yun-he, CHEN Xiu-ping, SONG Xin-yuan, SHEN Ping, PENG Yu-fa. 2019. No detrimental effect of Bt maize pollen containing Cry1Ab/2Aj or Cry1Ac on adult green lacewings Chrysoperla sinica Tjeder. Journal of Integrative Agriculture, 18(4): 893-899.

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