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Journal of Integrative Agriculture  2016, Vol. 15 Issue (9): 2059-2069    DOI: 10.1016/S2095-3119(16)61414-4
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Thrips-mediated impacts from transgenic rice expressing Cry1Ab on ecological fitness of non-target predator Orius tantilus (Hemiptera: Anthocoridae)
Akhtar Zunnu Raen1, 2, 3, DANG Cong1, WANG Fang1, PENG Yu-fa4, YE Gong-yin1
1 State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture/Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, P.R.China
2 Department of Entomology, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan                                                    
3 U.S. Pakistan Centre for Advance Studies in Agriculture Food Security (U.S.PCAS-AFS), University of Agriculture Faisalabad, Faisalabad 38000, Pakistan
4 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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Abstract      Various rice lines have been genetically modified with genes from Bacillus thuringiensis (Bt) to continuously produce Bt insecticidal proteins against lepidopteran larvae. The Bt insecticidal protein constantly expresses in the plants to create an opportunity for non-target herbivores to acquire and convey the protein to their predators or parasitoids across trophic levels. This paper evaluates the effects of Bt rice (namely, Kemindao 1 (KMD1) and Kemindao 2 (KMD2)) expressing Cry1Ab as compared to its non-Bt control line, Xiushui 11 on non-target predator Orius tantilus (a generalist predatory anthocorid of thrips) under laboratory and field conditions. To measure several biological parameters such as total nymphal duration and fecundity of this bug, it was reared on thrips and pollens of KMD1 and KMD2 as compared to their control under laboratory conditions. By comparison with the control, Bt rice did not significantly affect main life-history characteristics (total nymphal duration, female adult longevity, oviposition period and fecundity) of this anthocorid preying on Bt rice-fed thrips along with Bt rice pollens, except that the fecundity of this predator for KMD1 was distinctly lower as compared with KMD2 or the control. Enzyme-linked immunosorbent assay (ELISA) results showed that no Cry1Ab protein was detected in this predator fed on thrips or rice pollen from Bt rice but was in Bt rice pollens. With the beat plate, plastic bag and color trap sampling methods, two-year field monitoring of O. tantilus abundance demonstrated that Bt rice had no significant detrimental effects on the population dynamics and seasonal average densities of this predatory anthocorid as compared with the control. Thus, it is suggested that growing our tested Bt rice (KMD1 and KMD2) producing Cry1Ab will pose a negligible risk to the anthocorid, O. tantilus.
Keywords:  Orius tantilus        Bt rice        Cry1Ab        risk assessment        non-target effects  
Received: 25 October 2015   Accepted:

Financial supports were provided from the Special Research Projects for Developing Transgenic Plants, China (2014ZX08011-001) and the National Natural Science Fundation of China for Innovative Research Group (31321063), as well as the Rice Pests Management Research Group of the Agricultural Science and Technology Innovation Program, China.

Corresponding Authors:  YE Gong-yin, Tel: +86-571-88982696, Fax: +86-571-86049815, E-mail:   

Cite this article: 

Akhtar Zunnu Raen, DANG Cong, WANG Fang, PENG Yu-fa, YE Gong-yin. 2016. Thrips-mediated impacts from transgenic rice expressing Cry1Ab on ecological fitness of non-target predator Orius tantilus (Hemiptera: Anthocoridae). Journal of Integrative Agriculture, 15(9): 2059-2069.

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