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Journal of Integrative Agriculture  2025, Vol. 24 Issue (7): 2575-2588    DOI: 10.1016/j.jia.2024.02.015
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Effect of nitrogen fertilizer on proso millet starch structure, pasting, and rheological properties

Honglu Wang1, Hui Zhang1, Qian Ma1, Enguo Wu1, Aliaksandr Ivanistau2, Baili Feng1#

1 College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100, China

2 Belarusian State Agricultural Academy, Gorki 213407, Belarus

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摘要  
氮肥作为重要的农艺措施,对作物产量和品质有很大影响。本研究旨在探索四种氮肥水平对糜子淀粉的形态、片层和结晶结构、糊化和流变特性的影响,并揭示糜子的潜在食品应用价值。研究结果表明,随着施氮量增加,淀粉颗粒表面呈现凹凸不平状,淀粉颗粒的结构趋于复杂。施氮后提高了糜子淀粉的相对结晶度、有序结构和平均重复距离,使淀粉结构更加稳定,从而提高了淀粉的糊化焓。此外,施氮明显提高淀粉峰值粘度、崩解值、回生值和最终粘度,但降低了表观直链淀粉的含量,从而导致糜子淀粉基食品在加工和运输过程中抗剪切力和储存能力下降。流变学分析表明,糜子淀粉具有典型的假塑性流体特征。施氮后,糜子淀粉凝胶具有较强的抗剪切变稀能力、凝胶强度和屈服应力,在3D打印产品中具有良好的应用前景,这可归因于氮肥处理下糜子淀粉G′明显高于G′′,以及低直链淀粉含量可在水中形成稳定的网络结构以固定更多水分。我们的研究为糜子淀粉在食品工业中的应用以及糜子优质栽培提供参考。


Abstract  

Nitrogen fertilizer is an important agronomic measure significantly affecting crop yield and grain quality.  This two-year study aimed to explore the effects of four nitrogen levels on the morphology, lamellar and crystalline structure, pasting, and rheological properties of proso millet (PM) starch and to investigate potential food applications of PM.  The results showed that the starch surface became uneven, and the structure of the starch granules shifted towards greater complexity with increasing nitrogen levels.  Nitrogen increased the relative crystallinity, ordered structure, and average repeat distance, leading to a stable starch structure and a higher gelatinization enthalpy.  Furthermore, nitrogen significantly increased peak, breakdown, setback, and final viscosities but decreased apparent amylose content, which caused the shear resistance and storage capacity of PM starch-based foods to deteriorate during processing and transport.  Rheological analysis showed that PM starch exhibited the typical characteristics of a pseudoplastic fluid.  Under nitrogen treatment, PM starch gels showed high potential for application in 3D printing products due to high resistance to shear thinning, gel strength, and yield stress, presumably owing to the significantly higher G´ than G´´, and the lower amylose content, which in turn promoted the formation of a stable network structure in water to immobilize more water.  Our study provides a reference for applying PM starch in the food industry and for developing PM cropping-management programs to improve quality.


Keywords:  proso millet       nitrogen fertilizer        starch        amylose        structure  
Received: 18 September 2023   Online: 29 February 2024   Accepted: 03 January 2024
Fund: 
This work was supported by the Postdoctoral Fellowship Program of China Postdoctoral Science Foudation (CPSF) (GZC20241394), the Shaanxi Province Natural Science Basic Research Program-Youth Project, China (2025JC-YBQN271), the National Natural Science Foundation of China (31371529), the Shaanxi Province “Two Chains” Integrated Crop Breeding Key Project, China (2021LLRH-07), the Minor Grain Crops Research and Development System of Shaanxi Province, China (NYKJ-2021-YL(XN)40).

About author:  #Correspondence Baili Feng, E-mail: fengbaili@nwsuaf.edu.cn

Cite this article: 

Honglu Wang, Hui Zhang, Qian Ma, Enguo Wu, Aliaksandr Ivanistau, Baili Feng. 2025. Effect of nitrogen fertilizer on proso millet starch structure, pasting, and rheological properties. Journal of Integrative Agriculture, 24(7): 2575-2588.

Almeida V O, Batista K A, Di-Medeiros M C B, Moraes M G, Fernandes K F. 2019. Effect of drought stress on the morphological and physicochemical properties of starches from Trimezia juncifoliaCarbohydr Polymers212, 304–311.

Bangar S P, Ashogbon A O, Dhull S B, Thirumdas R, Kumar M, Hasan M, Chaudhary V, Pathem S. 2021. Proso-millet starch: Properties, functionality, and applications. International Journal of Biological Macromolecules190, 960–968.

Cai C, Lin L, Man J, Zhao L, Wang Z, Wei C. 2014. Different structural properties of high-amylose maize starch fractions varying in granule size. Journal of Agricultural and Food Chemistry62, 11711–11721.

Chao G, Gao J, Liu R, Wang L, Li C, Wang Y, Qu Y, Feng B. 2014. Starch physicochemical properties of waxy proso millet (Panicum miliaceum L.). Starch-Stärke66, 1005–1012.

Cheng Y, Liang K, Chen Y, Gao W, Kang X, Li T, Cui B. 2023. Effect of molecular structure changes during starch gelatinization on its rheological and 3D printing properties. Food Hydrocolloids137, 108364.

Deng F, Li Q, Chen H, Zeng Y, Li B, Zhong X, Wang L, Ren W. 2021. Relationship between chalkiness and the structural and thermal properties of rice starch after shading during grain-filling stage. Carbohydr Polymers252, 117212.

Gao L, Van Bockstaele F, Lewille B, Haesaert G, Eeckhout M. 2023. Characterization and comparative study on structural and physicochemical properties of buckwheat starch from 12 varieties. Food Hydrocolloids137, 108320.

Gao L, Wan C, Wang J, Wang P, Gao X, Eeckhout M, Gao J. 2022. Relationship between nitrogen fertilizer and structural, pasting and rheological properties on common buckwheat starch. Food Chemistry389, 132664.

Gulati P, Li A, Holding D, Santra D, Zhang Y, Rose D J. 2017. Heating reduces proso millet protein digestibility via formation of hydrophobic aggregates. Journal of Agricultural and Food Chemistry65, 1952–1959.

Jiang F, Feng J, Hu W, Ren Y, Ma C, Chang L, Liu Y, Lv X, Yu X, Du S K. 2022. Effects of morphology and rheology of starch nanoparticles prepared from various coarse cereals on emulsifying ability. Carbohydr Polymers298, 120137.

Lan X, Liu X, Yang Y, Wu J, Wang Z. 2017. The effect of lamellar structure ordering on the retrogradation properties of canna starch subjected to thermal and enzymatic degradation. Food Hydrocolloids69, 185–192.

Li B, Zhu L, Wang Y, Zhang Y, Huang C, Zhao Y, Xu F, Zhu K, Wu G. 2022. Multi-scale supramolecular structure of Pouteria campechiana (Kunth) Baehni seed and pulp starch. Food Hydrocolloids124, 107284.

Mirzababaee S M, Ozmen D, Hesarinejad M A, Toker O S, Yeganehzad S. 2022. A study on the structural, physicochemical, rheological and thermal properties of high hydrostatic pressurized pearl millet starch. International Journal of Biological Macromolecules223, 511–523.

Przetaczek-Roznowska I. 2017. Physicochemical properties of starches isolated from pumpkin compared with potato and corn starches. International Journal of Biological Macromolecules101, 536–542.

Singh N, Pal N, Mahajan G, Singh S, Shevkani K. 2011. Rice grain and starch properties: Effects of nitrogen fertilizer application. Carbohydrate Polymers86, 219–225.

Sun X, Sun Z, Saleh A S M, Zhao K, Ge X, Shen H, Zhang Q, Yuan L, Yu X, Li W. 2021. Understanding the granule, growth ring, blocklets, crystalline and molecular structure of normal and waxy wheat A- and B-starch granules. Food Hydrocolloids121, 107034.

Wang H, Li D, Ma Q, Wu E, Gao L, Yang P, Gao J, Feng B. 2023. Nitrogen fertilizer affects starch synthesis to define non-waxy and waxy proso millet quality. Carbohydrate Polymers302, 120423.

Wang H, Li Y, Wang L, Wang L, Li Z, Qiu J. 2022. Multi-scale structure, rheological and digestive properties of starch isolated from highland barley kernels subjected to different thermal treatments. Food Hydrocolloids129, 107630.

Wang H, Yang Q, Ferdinand U, Gong X, Qu Y, Gao W, Ivanistau A, Feng B, Liu M. 2020. Isolation and characterization of starch from light yellow, orange, and purple sweet potatoes. International Journal of Biological Macromolecules160, 660–668.

Xiong R, Tan X, Yang T, Pan X, Zeng Y, Huang S, Shang Q, Zhang J, Zeng Y. 2022. Relation of cooked rice texture to starch structure and physicochemical properties under different nitrogen managements. Carbohydrate Polymers295, 119882.

Yang Q, Zhang W, Li J, Gong X, Feng B. 2019. Physicochemical properties of starches in proso (non-waxy and waxy) and foxtail millets (non-waxy and waxy). Molecules24, 1743.

Yao D, Wu J, Luo Q, Li J, Zhuang W, Xiao G, Deng Q, Lei D, Bai B. 2020. Influence of high natural field temperature during grain filling stage on the morphological structure and physicochemical properties of rice (Oryza sativa L.) starch. Food Chemistry310, 125817.

Yuan Y, Liu C, Gao Y, Ma Q, Yang Q, Feng B. 2021. Proso millet (Panicum miliaceum L.): A potential crop to meet demand scenario for sustainable saline agriculture. Journal of Environmental Management296, 113216.

Zhang W, Yang Q, Xia M, Bai W, Wang P, Gao X, Gong X, Feng B, Gao L, Zhou M, Gao J. 2020. Effects of phosphate fertiliser on the physicochemical properties of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) starch. Food Chemistry307, 125543.

Zhang X, Feng J, Wang H, Zhu J, Zhong Y, Liu L, Xu S, Zhang R, Zhang X, Xue J, Guo D. 2018. Bivariate flow cytometric analysis and sorting of different types of maize starch grains. Cytometry (A), 93, 213–221.

Zhong Y, Qu J, Li Z, Tian Y, Zhu F, Blennow A, Liu X. 2022. Rice starch multi-level structure and functional relationships. Carbohydr Polymers275, 118777.

Zhou D, Yang G, Tian Y, Kang J, Wang S. 2023. Different effects of radio frequency and heat block treatments on multi-scale structure and pasting properties of maize, potato, and pea starches. Food Hydrocolloids136, 108306.

Zhou T, Zhou Q, Li E, Yuan L, Wang W, Zhang H, Liu L, Wang Z, Yang J, Gu J. 2020. Effects of nitrogen fertilizer on structure and physicochemical properties of ‘super’ rice starch. Carbohydr Polymers239, 116237.

Zhou T Y, Li Z K, Li E P, Wang W L, Yuan L M, Zhang H, Liu L J, Wang Z Q, Gu J Q, Yan J C. 2022. Optimization of nitrogen fertilization improves rice quality by affecting the structure and physicochemical properties of starch at high yield levels. Journal of Integrative Agriculture21, 1576–1592.

Zhu D, Zhang H, Guo B, Xu K, Dai Q, Wei C, Wei H, Gao H, Hu Y, Cui P, Huo Z. 2016. Effect of nitrogen management on the structure and physicochemical properties of rice starch. Journal of Agricultural and Food Chemistry64, 8019–8025.

Zhu D, Zhang H, Guo B, Xu K, Dai Q, Wei C, Zhou G, Huo Z. 2017. Effects of nitrogen level on structure and physicochemical properties of rice starch. Food Hydrocolloids63, 525–532.

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