Supplementation of Lycium barbarum residue increases the growth rate of Tan sheep by enhancing their feed intake and regulating their rumen microbiome and metabolome

doi:10.1016/j.jia.2023.10.008

Journal of Integrative Agriculture

2024, Vol. 23

Issue (9): 3129-3144 DOI: 10.1016/j.jia.2023.10.008

Animal Science · Veterinary Medicine

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Supplementation of Lycium barbarum residue increases the growth rate of Tan sheep by enhancing their feed intake and regulating their rumen microbiome and metabolome

Yajun Zhang¹, Xiao Chang², Bing Wang³, Dawei Wei⁴, Rongzhen Zhong², Yansheng Guo⁴, Min Du⁵, Guijie Zhang^{1, 4#}

¹ College of Forestry and Prataculture, Ningxia University, Yinchuan 750021, China
²Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agoecology, Chinese Academy of Sciences, Changchun 130102, China
³ State Key Laboratory of Animal Nutrition/College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
⁴College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China
⁵Nutrigenomics and Growth Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA

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摘要

本试验旨在研究枸杞渣对滩羊生长性能、瘤胃发酵参数、瘤胃微生物菌群及代谢组的影响。选取4月龄左右、体重[（20.8 ± 0.34）kg]相近和健康状况良好的滩羔羊16只，随机分为对照组和试验组，每组8只。对照组饲喂基础饲粮，试验组在基础饲粮中添加5%枸杞渣，每天饲喂2次。预试期10 d，正试期60 d。试验结束后，采集瘤胃液，测定发酵参数、瘤胃微生物群落结构及代谢产物。结果表明：1）与对照组相比，饲粮中添加5%枸杞渣显著增加了滩羊平均日采食量和平均日增重（P<0.05）。2）与对照组相比，饲粮中添加5%枸杞渣极显著增加了瘤胃液挥发性脂肪酸（P<0.01），显著降低了氨态氮含量和瘤胃液pH值（P<0.05）。3）多样性分析发现，2组间微生物Alpha多样性指数和Beta多样性差异显著（P<0.05）。微生物组成分析发现，在门水平上，试验组极显著增加了拟杆菌门、厚壁菌门、软壁菌门和蓝藻菌门的相对丰度（P<0.01），极显著降低了变形菌门、螺旋菌门和互养菌门的相对丰度（P<0.01）。在属水平上，添加枸杞渣显著增加了普雷沃菌属、琥珀酸菌属、瘤胃球菌属、粪球菌属、月形单胞菌属和丁酸弧菌属的相对丰度（P<0.05），显著降低了颤螺旋菌属和琥珀酸弧菌属的相对丰度（P<0.05）。4）通过代谢组学分析，共检测出431种差异代谢物。与对照组相比，试验组有287种差异代谢物显著上调，其中显著提高了氨基酸类（如L -脯氨酸、L-苯丙氨酸、L-赖氨酸和L -酪氨酸）、嘧啶代谢类（如尿嘧啶、尿嘧啶和胸腺嘧啶）和微生物蛋白合成类（如黄嘌呤和次黄嘌呤）等代谢产物的含量（P<0.05）。富集差异显著的代谢通路有嘧啶代谢，苯丙氨酸、酪氨酸和色氨酸的生物合成，精氨酸和脯氨酸代谢以及苯丙氨酸代谢。综上所述，饲粮中添加5%枸杞渣能改善滩羊生长性能，能够调节瘤胃菌群结构，影响瘤胃代谢物含量，改善瘤胃内环境。我们的研究结果表面了饲粮中添加枸杞渣对滩羊生长的促进作用，为其在反刍动物实际生产应用中提供了理论依据。

Abstract

Lycium barbarum residue (LBR), a by-product of L. barbarum processing, is packed with bioactive components and can be potentially utilized as a feed additive in animal husbandry. However, the fundamental understanding of its effectiveness on livestock animals is still lacking, particularly in ruminants. To explore the effects of LBR on the growth performance, rumen fermentation parameters, ruminal microbes and metabolites of Tan sheep, sixteen fattening rams (aged 4 mon) were fed a basal diet (CON, n=8) or a basal diet supplemented with 5% LBR (LBR, n=8). The experiment lasted for 70 d, with 10 d adaptation period and 60 d treatment period. The results showed that the LBR enhanced the average daily feed intake, average daily gain (P<0.05), and ruminal total volatile fatty acids (P<0.01) while decreasing ammonia-nitrogen concentration and rumen pH value (P<0.05). Additionally, the LBR improved the relative abundances of Prevotella, Succiniclasticum, Ruminococcus, Coprococcus, Selenomonas, and Butyrivibrio (P<0.05) and reduced the relative abundances of Oscillospira and Succinivibrio (P<0.05). The LBR altered the ruminal metabolome (P<0.01) by increasing the abundances of ruminal metabolites involved in amino acids (e.g., L-proline, L-phenylalanine, L-lysine, and L-tyrosine), pyrimidine metabolism (e.g., uridine, uracil, and thymidine), and microbial protein synthesis (e.g., xanthine and hypoxanthine). In conclusion, LBR had positive effects on the growth rate of Tan sheep as well as on rumen fermentation parameters, rumen microbiome and rumen metabolome.

Keywords: Lycium barbarum residue rumen microbiome metabolomics growth performance Tan sheep

Received: 17 February 2023 Accepted: 27 July 2023

Fund:

This study was supported by the National Key Research and Development Program of China (2022YFD1300905), the National Natural Science Foundation of China (31960672), the Key Research and Development Program of Ningxia Hui Autonomous Region, China (2021BEF02020), the Top Discipline Construction Project of Pratacultural Science (NXYLXK2017A01), the Science and Technology Development Project of Jilin Province, China (20200201140JC), and the Technology Cooperation High-Tech Industrialization Project of Jilin Province, China and the Chinese Academy of Sciences, (2022SYHZ0020).

About author: Yajun Zhang, E-mail: zhangyajun1106@126.com; #Correspondence Guijie Zhang, Tel: +86-951-2062861, E-mail: guijiezhang@nxu.edu.cn

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Yajun Zhang, Xiao Chang, Bing Wang, Dawei Wei, Rongzhen Zhong, Yansheng Guo, Min Du, Guijie Zhang. 2024. Supplementation of Lycium barbarum residue increases the growth rate of Tan sheep by enhancing their feed intake and regulating their rumen microbiome and metabolome. Journal of Integrative Agriculture, 23(9): 3129-3144.

Ametaj B N, Zebeli Q, Saleem F, Psychogios N, Lewis M J, Dunn S M, Xia J, Wishart D S. 2010. Metabolomics reveals unhealthy alterations in rumen metabolism with increased proportion of cereal grain in the diet of dairy cows. Metabolomics, 6, 583–594.

Andries J I, Buysse F X, De Brabander D L, Cottyn B G. 1987. Isoacids in ruminant nutrition: Their role in ruminal and intermediary metabolism and possible influences on performances – A review. Animal Feed Science and Technology, 18, 169–180.

AOAC (Association of Official Analytical Chemists). 2000. Official Methods of Analysis. 17th ed. The Association of Official Analytical Chemists, International, Arlington.

Bach A, Calsamiglia S, Stern M D. 2005. Nitrogen metabolism in the rumen. Journal of Dairy Science, 88, E9–E21.

Bokulich N A, Kaehler B D, Rideout J R, Dillon M, Bolyen E, Knight R, Huttley G A ,Gregory Caporaso J. 2018. Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin. Microbiome, 6, 1–17.

Brulc J M, Antonopoulos D A, Berg Miller M E, Wilson M K, Yannarell A C, Dinsdale E A, Edwards R E, Frank E D, Emerson J B, Wacklin P, Coutinho P M, Henrissat B, Nelson K E, White B A. 2009. Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. Proceedings of the National Academy of Sciences of the United States of America, 106, 1948–1953.

Carberry C A, Kenny D A, Han S, McCabe M S, Waters S M. 2012. Effect of phenotypic residual feed intake and dietary forage content on the rumen microbial community of beef cattle. Applied and Environmental Microbiology, 78, 4949–4958.

Castanon J I R. 2007. History of the use of antibiotic as growth promoters in European poultry feeds. Poultry Science, 86, 2466–2471.

Ceconi I, Ruiz-Moreno M J, Dilorenzo N, Dicostanzo A, Crawford G I. 2015. Effect of urea inclusion in diets containing corn dried distillers grains on feedlot cattle performance, carcass characteristics, ruminal fermentation, total tract digestibility, and purine derivatives-to-creatinine index. Journal of Animal Science, 93, 357–369.

Chen H, Guo B, Yang M, Luo J, Hu Y, Qu M, Song X. 2021. Response of growth performance, blood biochemistry indices, and rumen bacterial diversity in lambs to diets containing supplemental probiotics and Chinese medicine polysaccharides. Frontiers in Veterinary Science, 8, 681389.

Chen J, Long L, Jiang Q, Kang B, Li Y, Yin J. 2020. Effects of dietary supplementation of Lycium barbarum polysaccharides on growth performance, immune status, antioxidant capacity and selected microbial populations of weaned piglets. Journal of Animal Physiology and Animal Nutrition, 104, 1106–1115.

Cheng D, Kong H. 2011. The effect of Lycium barbarum polysaccharide on alcohol-induced oxidative stress in rats. Molecules, 16, 2542–2550.

Clemmons B A, Powers J B, Campagna S R, Seay T B, Embree M M, Myer P R. 2020. Rumen fluid metabolomics of beef steers differing in feed efficiency. Metabolomics, 16, 1–9.

Cremonesi P, Curone G, Biscarini F, Cotozzolo E, Menchetti L, Riva F, Marongiu M L, Castiglioni B, Barbato O, Munga A, Castrica M, Vigo D, Sulce M, Quattrone A, Agradi S, Brecchia G. 2022. Dietary Supplementation with Goji Berries (Lycium barbarum) modulates the microbiota of digestive tract and caecal metabolites in rabbits. Animals, 12, 121.

Dijkstra J, Ellis J L, Kebreab E, Strathe A B, López S, France J, Bannink A. 2012. Ruminal pH regulation and nutritional consequences of low pH. Animal Feed Science and Technology, 172, 22–33.

Dunn W B, Broadhurst D, Begley P, Zelena E, Francis-Mcintyre S, Anderson N, Brown M, Knowles J D, Halsall A, Haselden J N, Nicholls A W, Wilson I D, Kell D B, Goodacre R. 2011. Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nature Protocols, 6, 1060–1083.

Emerson E L, Weimer P J. 2017. Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures. Applied Microbiology and Biotechnology, 101, 4269–4278.

Farghaly M M, Abdullah M A M, Youssef I M I, Abdel-Rahim I R, Abouelezz K. 2019. Effect of feeding hydroponic barley sprouts to sheep on feed intake, nutrient digestibility, nitrogen retention, rumen fermentation and ruminal enzymes activity. Livestock Science, 228, 31–37.

Fernando S C, Purvis H T, Najar F Z, Sukharnikov L O, Krehbiel C R, Nagaraja T G, Roe B A, De Silva U. 2010. Rumen microbial population dynamics during adaptation to a high-grain diet. Applied and Environmental Microbiology, 76, 7482–7490.

Flint H J, Scott K P, Duncan S H, Louis P, Forano E. 2012. Microbial degradation of complex carbohydrates in the gut. Gut Microbes, 3, 289–306.

Gao Y, Wei Y, Wang Y, Gao F, Chen Z. 2017. Lycium barbarum: A traditional Chinese herb and a promising anti-aging agent. Aging and Disease, 8, 778–791.

He Y, Qiu Q, Shao T, Niu W, Xia C, Wang H, Li Q, Gao Z, Yu Z, Su H, Cao B. 2017. Dietary alfalfa and calcium salts of long-chain fatty acids alter protein utilization, microbial populations, and plasma fatty acid profile in Holstein freemartin heifers. Journal of Agricultural and Food Chemistry, 65, 10859–10867.

Hippe B, Remely M, Aumueller E, Pointner A, Haslberger A G. 2015. SCFA producing gut microbiota and its effects on the epigenetic regulation of inflammation. Beneficial Microorganisms in Medical and Health Applications, 28, 181–197.

Hu W L, Liu J X, Ye J A, Wu Y M, Guo Y Q. 2005. Effect of tea saponin on rumen fermentation in vitro. Animal Feed Science and Technology, 120, 333–339.

Ji Y, Guo Q, Yin Y, Blachier F, Kong X. 2018. Dietary proline supplementation alters colonic luminal microbiota and bacterial metabolite composition between days 45 and 70 of pregnancy in Huanjiang mini-pigs. Journal of Animal Science and Biotechnology, 9, 1–11.

Kajikawa H, Mitsumori M, Ohmomo S. 2002. Stimulatory and inhibitory effects of protein amino acids on growth rate and efficiency of mixed ruminal bacteria. Journal of Dairy Science, 85, 2015–2022.

Klevenhusen F, Petri R M, Kleefisch M T, Khiaosa-ard R, Metzler-Zebeli B U, Zebeli Q. 2017. Changes in fibre-adherent and fluid-associated microbial communities and fermentation profiles in the rumen of cattle fed diets differing in hay quality and concentrate amount. FEMS Microbiology Ecology, 93, fix100.

Kopečný J, Zorec M, Mrázek J, Kobayashi Y, Marinšek-Logar R. 2003. Butyrivibrio hungatei sp. nov. and Pseudobutyrivibrio xylanivorans sp. nov., butyrate-producing bacteria from the rumen. International Journal of Systematic and Evolutionary Microbiology, 53, 201–209.

Li F, Li Z, Li S, Ferguson J D, Yang T. 2014. Effect of dietary physically effective fiber on ruminal fermentation and the fatty acid profile of milk in dairy goats. Journal of Dairy Science, 97, 2281–2291.

Lian Y Z, Lin I H, Yang Y C, Chao J C J. 2020. Gastroprotective effect of Lycium barbarum polysaccharides and C-phyocyanin in rats with ethanol-induced gastric ulcer. International Journal of Biological Macromolecules, 165, 1519–1528.

Liu Y L, Yin R Q, Liang S S, Duan Y L, Yao J H, Duan Y L, Yang X J. 2017. Effect of dietary Lycium barbarum polysaccharide on growth performance and immune function of broilers. Journal of Applied Poultry Research, 26, 200–208.

Long L N, Kang B J, Jiang Q, Chen J S. 2020. Effects of dietary Lycium barbarum polysaccharides on growth performance, digestive enzyme activities, antioxidant status, and immunity of broiler chickens. Poultry Science, 99, 744–751.

Lu Y, Guo S, Zhang F, Yan H, Qian D W, Wang H Q, Jin L, Duan J A. 2019. Comparison of functional components and antioxidant activity of lycium barbarum L. fruits from different regions in China. Molecules, 24, 2228.

Ma X K, Shang Q H, Wang Q Q, Hu JX, Piao X S. 2019. Comparative effects of enzymolytic soybean meal and antibiotics in diets on growth performance, antioxidant capacity, immunity, and intestinal barrier function in weaned pigs. Animal Feed Science and Technology, 248, 47–58.

Mariz L D S, Amaral P M, Valadares Filho S C, Santos S A, Detmann E, Marcondes M I, Pereira J M V, Silva Júnior J M, Prados L F, Faciola A P. 2018. Dietary protein reduction on microbial protein, amino acid digestibility, and body retention in beef cattle: 2. amino acid intestinal absorption and their efficiency for whole-body deposition. Journal of Animal Science, 96, 670–683.

Martin M. 2011. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet Journal, 17, 10–12.

Menchetti L, Curone G, Andoni E, Barbato O, Troisi A, Fioretti B, Polisca A, Codini M, Canali C, Vigo D, Brecchia G. 2020. Impact of goji berries (Lycium barbarum) supplementation on the energy homeostasis of rabbit does: Uni- and multivariate approach. Animals, 10, 2000.

Menchetti L, Vecchione L, Filipescu I, Petrescu V F, Fioretti B, Beccari T, Ceccarini M R, Codini M, Quattrone A, Trabalza-Marinucci M, Barbato O, Brecchia G. 2019. Effects of Goji berries supplementation on the productive performance of rabbit. Livestock Science, 220, 123–128.

Qiao J, Li H H, Zheng C J, Feng Z Y, Wang W. 2013. Dietary supplementation with Aloe vera polysaccharide enhances the growth performance and immune function of weaned piglets. Journal of Animal and Feed Sciences, 22, 329–334.

Ruíz-Salinas A K, Vázquez-Roque R A, Díaz A, Pulido G, Treviño S, Floran B, Flores G. 2020. The treatment of Goji berry (Lycium barbarum) improves the neuroplasticity of the prefrontal cortex and hippocampus in aged rats. The Journal of Nutritional Biochemistry, 83, 108416.

Saleem F, Bouatra S, Guo A C, Psychogios N, Mandal R, Dunn S M, Ametaj B N, Wishart D S. 2013. The bovine ruminal fluid metabolome. Metabolomics, 9, 360–378.

Shen J, Chen Y, Moraes L E, Yu Z, Zhu W. 2018. Effects of dietary protein sources and nisin on rumen fermentation, nutrient digestion, plasma metabolites, nitrogen utilization, and growth performance in growing lambs. Journal of Animal Science, 96, 1929–1938.

Singh K M, Reddy B, Patel A K, Panchasara H, Parmar N, Patel A B, Shah T M, Bhatt V D, Joshi C G. 2014. Metagenomic analysis of buffalo rumen microbiome: Effect of roughage diet on Dormancy and Sporulation genes. Meta Gene, 2, 252–268.

Smith C A, Want E J, O’Maille G, Abagyan R, Siuzdak G. 2006. XCMS: Processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. Analytical Chemistry, 78, 779–787.

Van Soest P J, Robertson J B, Lewis B A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597.

Sóki J, Wybo I, Hajdú E, Toprak N U, Jeverica S, Stingu C S, Tierney D, Perry J D, Matuz M, Urbán E, Nagy E. 2020. A Europe-wide assessment of antibiotic resistance rates in Bacteroides and Parabacteroides isolates from intestinal microbiota of healthy subjects. Anaerobe, 62, 102182.

Tu Y, Zhang G F, Deng K D, Zhang N F, Diao Q Y. 2015. Effects of supplementary bee pollen and its polysaccharides on nutrient digestibility and serum biochemical parameters in Holstein calves. Animal Production Science, 55, 1318–1323.

Wang B, Ma M P, Diao Q Y, Tu Y. 2019. Saponin-induced shifts in the rumen microbiome and metabolome of young cattle. Frontiers in Microbiology, 10, 356.

Wang Y, Nan X, Zhao Y, Jiang L, Wang H, Hua D, Zhang F, Wang Y, Liu J, Yao J, Xiong B. 2021. Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows. Animal Nutrition, 7, 1189–1204.

Yi R, Liu X M, Dong Q. 2013. A study of Lycium barbarum polysaccharides (LBP) extraction technology and its anti-aging effect. African Journal of Traditional, Complementary and Alternative Medicines, 10, 171–174.

Yin F, Yin Y, Kong X, Liu Y, He Q, Li T, Huang R, Hou Y, Shu X, Tan L, Chen L, Gong J, Kim S W, Wu G. 2008. Dietary supplementation with Acanthopanax senticosus extract modulates gut microflora in weaned piglets. Asian-Australasian Journal of Animal Sciences, 21, 1330–1338.

Yin F G, Liu Y L, Yin Y L, Kong X F, Huang R L, Li T J, Wu G Y, Hou Y. 2009. Dietary supplementation with Astragalus polysaccharide enhances ileal digestibilities and serum concentrations of amino acids in early weaned piglets. Amino Acids, 37, 263–270.

Yu S, Shi W, Yang B, Gao G, Chen H, Cao L, Yu Z, Wang J. 2020. Effects of repeated oral inoculation of artificially fed lambs with lyophilized rumen fluid on growth performance, rumen fermentation, microbial population and organ development. Animal Feed Science and Technology, 264, 114465.

Zhang J, Shi H, Wang Y, Li S, Cao Z, Ji S, He Y, Zhang H. 2017. Effect of dietary forage to concentrate ratios on dynamic profile changes and interactions of ruminal microbiota and metabolites in Holstein heifers. Frontiers in Microbiology, 8, 2206.

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