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Journal of Integrative Agriculture  2022, Vol. 21 Issue (11): 3158-3168    DOI: 10.1016/j.jia.2022.07.055
Special Issue: 玉米遗传育种合辑Maize Genetics · Breeding · Germplasm Resources
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Cytological study on haploid male fertility in maize
YANG Ji-wei1, 2*, LIU Zong-hua1*, QU Yan-zhi1, ZHANG Ya-zhou1, LI Hao-chuan1
1 College of Agronomy, Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science/Henan Grain Crop Collaborative Innovation Center, Zhengzhou 450046, P.R.China
2 Institute of Cereal Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China
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Abstract  Doubled haploid (DH) breeding technology, which relies on haploid genome doubling, is widely used in commercial maize breeding.  Spontaneous haploid genome doubling (SHGD), a more simplified and straightforward method, is gaining popularity among maize breeders.  However, the cytological mechanism of SHGD remains unclear.  This study crossed inbred lines RL36 and RL7, which have differing SHGD abilities, with inducer line YHI-1 to obtain haploid kernels.  The meiotic processes of pollen mother cells (PMCs) in the haploid plants were compared with diploid controls.  The results suggested that three main pathways, the early doubling of haploid PMCs, the first meiotic metaphase chromosomal segregation distortion, and anomaly of the second meiosis, are responsible for SHGD.  Furthermore, flow cytometry analysis of ploidy levels in leaves and PMCs from haploids and diploid controls revealed that somatic cell chromosome doubling and germ cell chromosome doubling are independent processes.  These findings provide a foundation for further studies on the underlying mechanism of SHGD, aiding the application of DH technology in maize breeding practices.  
Keywords:  maize       haploid         male fertility        spontaneous genome doubling        meiosis  
Received: 31 March 2021   Accepted: 07 June 2021
Fund: This work was supported by the Agricultural Seed Joint Research Project of Henan Province, China (2022010202), the Science and Technology Project of Henan Province, China (222102110276) and the China Postdoctoral Science Foundation (2020M682031).
About author:  YANG Ji-wei, E-mail:; LIU Zong-hua, E-mail:; Correspondence LI Hao-chuan, Tel: +86-371-56990188, E-mail: * These authors contributed equally to this study.

Cite this article: 

YANG Ji-wei, LIU Zong-hua, QU Yan-zhi, ZHANG Ya-zhou, LI Hao-chuan. 2022. Cytological study on haploid male fertility in maize. Journal of Integrative Agriculture, 21(11): 3158-3168.

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