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Special Issue:
玉米遗传育种Maize Genetics · Breeding · Germplasm Resources
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| Cytological study on haploid male fertility in maize |
| YANG Ji-wei1, 2*, LIU Zong-hua1*, QU Yan-zhi1, ZHANG Ya-zhou1, LI Hao-chuan1 |
1 College of Agronomy, Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science/Henan Grain Crop Collaborative Innovation Center, Zhengzhou 450046, P.R.China
2 Institute of Cereal Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China
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摘要
玉米单倍体育种技术主要取决于单倍体基因组加倍，并已广泛应用于商业育种之中。单倍体基因组自然加倍（SHGD）是一种简单、快捷的方法，并在育种者中越来越受欢迎。但目前SHGD的细胞学机制仍不清楚。为此，我们利用诱导系YHI-1与两个极端SHGD能力的重组自交系RL36和RL7进行杂交，得到单倍体籽粒。对单倍体植物中花药减数分裂过程中花粉母细胞（PMC）与对应的二倍体花粉母细胞进行了比较。结果表明：早期加倍、第一次减数分裂中期染色体偏分离及第二次减数分裂异常三个主要的途径与SHGD有关。此外，对单倍体和二倍体植株的叶片及PMC利用流式细胞仪进行倍性分析，结果表明：单倍体植株体细胞染色体加倍和生殖细胞染色体加倍是相对独立的过程。这些结果为进一步研究SHGD的可能机制提供了基础，将有助于单倍体育种技术在玉米育种实践中应用。
Abstract Doubled haploid (DH) breeding technology, which relies on haploid genome doubling, is widely used in commercial maize breeding. Spontaneous haploid genome doubling (SHGD), a more simplified and straightforward method, is gaining popularity among maize breeders. However, the cytological mechanism of SHGD remains unclear. This study crossed inbred lines RL36 and RL7, which have differing SHGD abilities, with inducer line YHI-1 to obtain haploid kernels. The meiotic processes of pollen mother cells (PMCs) in the haploid plants were compared with diploid controls. The results suggested that three main pathways, the early doubling of haploid PMCs, the first meiotic metaphase chromosomal segregation distortion, and anomaly of the second meiosis, are responsible for SHGD. Furthermore, flow cytometry analysis of ploidy levels in leaves and PMCs from haploids and diploid controls revealed that somatic cell chromosome doubling and germ cell chromosome doubling are independent processes. These findings provide a foundation for further studies on the underlying mechanism of SHGD, aiding the application of DH technology in maize breeding practices.
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Received: 31 March 2021
Accepted: 07 June 2021
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| Fund: This work was supported by the Agricultural Seed Joint Research Project of Henan Province, China (2022010202), the Science and Technology Project of Henan Province, China (222102110276) and the China Postdoctoral Science Foundation (2020M682031). |
| About author: YANG Ji-wei, E-mail: yangjiwei2009@126.com; LIU Zong-hua, E-mail: zhliu100@163.com; Correspondence LI Hao-chuan, Tel: +86-371-56990188, E-mail: lihaochuan1220@163.com
* These authors contributed equally to this study. |
Cite this article:
YANG Ji-wei, LIU Zong-hua, QU Yan-zhi, ZHANG Ya-zhou, LI Hao-chuan.
2022.
Cytological study on haploid male fertility in maize. Journal of Integrative Agriculture, 21(11): 3158-3168.
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