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PpMAPK6 regulates peach bud endodormancy release through interactions with PpDAM6
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ZHANG Yu-zheng1, 2, 3, XU Chen1, 2, 3, LU Wen-li1, 2, 3, WANG Xiao-zhe1, 2, 3, WANG Ning1, 2, 3, MENG Xiang-guang1, 2, 3, FANG Yu-hui1, 2, 3, TAN Qiu-ping1, 2, 3, CHEN Xiu-de1, 2, 3, FU Xi-ling1, 2, 3, LI Ling1, 2, 3
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1 College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
3 Shandong Collaborative Innovation Center for Fruit & Vegetable Production with High Quality and Efficiency, Tai’an 271018, P.R.China
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摘要
休眠相关的MADS-box (DAM)基因PpDAM6在芽休眠解除过程中起关键作用，其在休眠解除过程中表达量降低。然而，其在调控桃花芽内休眠解除中的互作网络仍不够完善。在本研究中，我们使用酵母双杂交(Y2H)技术，从桃休眠相关SSHcDNA文库中鉴定到了一种丝裂原活化蛋白激酶PpMAPK6，它与PpDAM6相互作用。PpMAPK6主要位于细胞核中。进一步的Y2H和双分子荧光互补(BiFC)实验验证了PpMAPK6通过结合PpDAM6的MADS-box结构域与PpDAM6相互作用。实时荧光定量PCR (qRT-PCR)分析结果表明，在3个不同需冷量的桃品种(春捷，中油5号，青州蜜桃)中PpMAPK6的表达趋势与PpDAM6相反。此外，ABA抑制PpMAPK6在花芽中的表达，促进PpDAM6在花芽中的表达。结果表明，PpMAPK6可能通过与PpDAM6相互作用使其磷酸化，从而加速其降解。在桃花芽内休眠解除过程中随着ABA含量的降低，PpMAPK6的表达量增加，进而降低了PpDAM6的表达量，促进桃花芽内休眠解除。
Abstract
The MADS-box (DAM) gene PpDAM6, which is related to dormancy, plays a key role in bud endodormancy release, and the expression of PpDAM6 decreases during endodormancy release. However, the interaction network that governs its regulation of the endodormancy release of flower buds in peach remains unclear. In this study, we used yeast two-hybrid (Y2H) assays to identify a mitogen-activated protein kinase, PpMAPK6, that interacts with PpDAM6 in a peach dormancy-associated SSHcDNA library. PpMAPK6 is primarily located in the nucleus, and Y2H and bimolecular fluorescence complementation (BiFC) assays verified that PpMAPK6 interacts with PpDAM6 by binding to the MADS-box domain of PpDAM6. Quantitative real-time PCR (qRT-PCR) analysis showed that the expression of PpMAPK6 was opposite that of PpDAM6 in the endodormancy release of three cultivars with different chilling requirements (Prunus persica ‘Chunjie’, Prunus persica var. nectarina ‘Zhongyou 5’, Prunus persica ‘Qingzhou peach’). In addition, abscisic acid (ABA) inhibited the expression of PpMAPK6 and promoted the expression of PpDAM6 in flower buds. The results indicated that PpMAPK6 might phosphorylate PpDAM6 to accelerate its degradation by interacting with PpDAM6. The expression of PpMAPK6 increased with decreasing ABA content during endodormancy release in peach flower buds, which in turn decreased the expression of PpDAM6 and promoted endodormancy release.
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Received: 07 February 2022
Accepted: 25 August 2022
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Fund: This work was partially supported by the National Key Research and Development Plan (2018YFD1000104), the National Natural Science Foundation of China (318720415) and the Agricultural Improved Seed Project Grant of Shandong, China (2020LZGC007 and 2020LZGC00702) and the Fruit Industry Technology System Project of Shandong, China (SDAIT-06-04).
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| About author: ZHANG Yu-zheng, E-mail: 2227144914@qq.com; Correspondence FU Xi-ling, E-mail: xilingfu@sdau.edu.cn; LI Ling, E-mail: liling217@sdau.edu.cn |
Cite this article:
ZHANG Yu-zheng, XU Chen, LU Wen-li, WANG Xiao-zhe, WANG Ning, MENG Xiang-guang, FANG Yu-hui, TAN Qiu-ping, CHEN Xiu-de, FU Xi-ling, LI Ling.
2023.
PpMAPK6 regulates peach bud endodormancy release through interactions with PpDAM6
. Journal of Integrative Agriculture, 22(1): 139-148.
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